Detection of mRNAs associated with a eIF2α-P immunoprecipitated protein fraction. The translation initiation factor eIF2α-P was immunoprecipitated from whole cell protein extracts of wild type (wt) and myo1Δ strains. Total RNA was extracted from the immunoprecipitated fractions and RT-PCR analysis of mRNAs for RPS8A, RPL7B, RPL3 (left panels) and ACT1, CHS4, PIR3 (right panels) was performed. Mock immunoprecipitations were performed as a negative control for each experiment as described in Figure 7.
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