Visualization of P-bodies in vivo. Wild type (wt) and myo1Δ yeast strains were transformed with a plasmid encoding a recombinant form of decapping enzyme Dcp2p tagged with the fluorescent probe mCherry (Dcp2p-mCh). Transformed strains were grown in CSM broth with glucose (+ GLU) and in CSM broth without glucose (-GLU). Upper panels represent light micrographs and lower panels represent the corresponding u.v. fluorescence micrographs for each condition. All images were captured at the same exposure time and magnification (100×). The graph below shows a quantification of the average number of P- bodies per 100 cells. See Methods for details.
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