Analysis of polysome profiles. Equivalent amounts of total cell lysate from A) wild-type (wt) and B) myo1Δ strains were loaded onto 10-50% sucrose gradients. The u.v. absorbance of pooled sequential gradient fractions (numbered consecutively) was measured at 260 nm (see Methods for details). The 40 S, 60 S, and 80 S fractions were classified as non-polysome fractions. All subsequent fractions were classified as polysome fractions. Polysome peaks were numbered consecutively according to their fraction number (4-9). Total RNA extracted from each of the fractions was subjected to electrophoretic analysis of ribosomal RNAs (25 S and 18S).
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