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Logo of bmcpharBioMed Centralsearchsubmit a manuscriptregisterthis articleBMC Pharmacology
 
BMC Pharmacol. 2010; 10(Suppl 1): A17.
Published online 2010 November 16. doi:  10.1186/1471-2210-10-S1-A17
PMCID: PMC3016526

A biological target for antiplatelet therapy: the prostaglandin E2 receptor EP4

Background

Acute myocardial infarction is one of the leading causes of death in the world which is caused by coronary artery thrombosis. Platelets play a central role in cardiovascular thrombosis. Platelet aggregation caused due to a ruptured artherosclerotic plaque could eventually lead to vascular occlusion. Another important component of vascular diseases is inflammation. During inflammation, prostaglandins (PG) like PGI2, PGE2 and PGD2 are released which are also involved in thrombosis. Lower concentrations of PGE2 enhance platelet aggregation whereas higher concentrations inhibit aggregation. PGE2 acts via 4 receptors: EP1, EP2, EP3 and EP4 (Gs signalling). The role of the EP3 receptor in enhancing platelet activation and aggregation has been looked at in detail but the role of the EP4 receptor is largely unknown. We were interested in how this receptor modulates platelet aggregation and what are the signalling mechanisms involved in this process.

Methods

Platelet aggregation assays were performed ex vivo using a platelet aggregation analyser (Aggregometer II). Blood from healthy human donors was used to obtain platelet-rich plasma. Aggregation was induced using ADP or collagen. Different agonists and antagonists were added to investigate their effects on platelet aggregation. Ca2+ flux changes caused by addition of agonists were also examined using a fluorescent Ca2+ dye (Fluo-3) by flow cytometry. Expression of the EP4 receptor on the surface of platelets was established using indirect flow cytometry whereas expression of CD62P, PAC1 and CD41 was examined using direct flow cytometry. In vitro thrombus formation was assessed by flowing whole blood on collagen-coated Cellix biochips at −30 dyne/cm2 using the Mirus nanopump.

Results

We observed that human platelets express EP4 receptors. A selective EP4 agonist potently inhibited the platelet aggregation as induced by ADP or collagen. This effect could be completely reversed by using an EP4 antagonist, but not by PGI2, PGD2 TXA2 receptor antagonists. Moreover, an EP4 antagonist enhanced the PGE2-induced stimulation of platelet aggregation, indicating a potent anti-aggregatory activity of the EP4 receptors. Interestingly, the inhibitory effect of the EP4 agonist was brought about by protein kinase C but not adenylyl cyclase, accompanied by attenuated Ca2+ flux, decreased activation of glycoprotein IIb/IIIa and down-regulation of P-selectin. Most importantly, in vitro thrombus formation was effectively reduced by the EP4 agonist and this effect was reversed using the EP4 antagonist.

Conclusions

These findings indicate that the EP4 receptor is a potential biological drug target in anti-platelet therapy.


Articles from BMC Pharmacology are provided here courtesy of BioMed Central