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Rescue of a quadruple knockout E. coli by co-expression of 4 de novo proteins.
(A) Schematic of knockout rescue. Top: Wild-type E. coli transformed with empty vector forms colonies on M9/glucose minimal media. Middle: QUAD E. coli transformed with empty vector does not form colonies on minimal media. Bottom: QUAD transformed with a vector co-expressing four de novo sequences forms colonies on minimal media. (B) Colonies are not observed when QUAD is transformed with empty vector (top panel), but are observed when transformed with a vector expressing four artificial genes (bottom panel). Scale bar=1 mm.