PMCCPMCCPMCC

Search tips
Search criteria 

Advanced

 
Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
Front Neuroendocrinol. Author manuscript; available in PMC 2012 January 1.
Published in final edited form as:
PMCID: PMC3012750
NIHMSID: NIHMS227401

THE NEUROBIOLOGY OF PAIR BONDING: INSIGHTS FROM A SOCIALLY MONOGAMOUS RODENT

Abstract

The formation of enduring relationships between adult mates (i.e., pair bonds) is an integral aspect of human social behavior and has been implicated in both physical and psychological health. However, due to the inherent complexity of these bonds and the relative rarity with which they are formed in other mammalian species, we know surprisingly little about their underlying neurobiology. Over the past few decades, the prairie vole (Microtus ochrogaster) has emerged as an animal model of pair bonding. Research in this socially monogamous rodent has provided valuable insights into the neurobiological mechanisms that regulate pair bonding behaviors. Here, we review these studies and discuss the neural regulation of three behaviors inherent to pair bonding: the formation of partner preferences, the subsequent development of selective aggression toward unfamiliar conspecifics, and the bi-parental care of young. We focus on the role of vasopressin, oxytocin, and dopamine in the regulation of these behaviors, but also discuss the involvement of other neuropeptides, neurotransmitters, and hormones. These studies may not only contribute to the understanding of pair bonding in our own species, but may also offer insight into the underlying causes of social deficits noted in several mental health disorders.

Keywords: Social attachment, aggression, paternal behavior, vasopressin, oxytocin, dopamine, prairie vole, monogamy

1. Introduction

Intense attraction between mates, often referred to as romantic or passionate love, is one of the most powerful forces driving human social behavior, and often precedes the formation of enduring, selective attachments between sexual partners (i.e., pair bonds). Although such sociosexual attachments are most prevalent in industrialized cultures with a monogamous social organization, they occur in nearly all human societies, regardless of subsistence mode (e.g., pastoralist, agriculturalist, etc.) or mating strategy (e.g., polygamy and monogamy), and are therefore an intrinsic part of human social behavior. While the definition of a pair bond varies throughout the literature, it is typically described, across species, as an enduring preferential association formed between two sexually mature adults, and is characterized by selective contact, affiliation, and copulation with the partner over a stranger (partner preference) [105]. In addition to a preference for a partner, a variety of other behaviors are intrinsically involved in this complex social bond. For example, pair bonds in humans, as well as in other mammalian species, are regularly associated with mate-guarding (e.g., highly aggressive behavior towards sexual competitors) and the bi-parental care of young [32, 86, 136]. The co-occurrence of these behaviors in pair-bonded individuals makes sense when viewed through the lens of evolutionary theory, which suggests, in part, that pair bonding became adaptive under conditions in which additional parental investment was required to ensure the successful rearing of young [45, 85, 89, 105, 208]. Indeed, the same selection pressures that necessitated the presence of both parents for offspring survival would likely facilitate the formation of a partnership between mates [86] and mechanisms through which to maintain this partnership (e.g., mate-guarding).

The functional significance of pair bonding in humans has been documented cross-culturally. Paired individuals, particularly those in stable marital relationships, live longer than their unpaired counterparts, a finding noted across demographic groups [116, 144]. Additionally, high levels of intimacy between pairs has been inversely correlated with negative psychological states, such as depressed mood, and positively correlated with immune function and cardiovascular health [131, 212]. Another widely acknowledged benefit of pair bonding in humans, as in other species, is the physical and psychological well-being of children, an effect likely due to the co-occurrence of pair bonding with the bi-parental care of young. Indeed, paternal involvement in childcare has become increasingly recognized as equally important as maternal influences on successful childhood development. In preindustrial societies and developing countries, for example, where food and healthcare are not readily available, children of monogamously married women have lower mortality rates than children of women who are not married or who are in a polygynous union [206]. In industrialized societies, the presence of caring fathers improves the emotional and cognitive health and development of children, as indicated by higher levels of child success on various indices, including academic achievement [41, 71, 83, 88, 181, 191] and the prevention and treatment of anxiety problems [28], attention-deficit/hyperactivity disorder (ADHD) [75], substance use, and criminal behavior [200].

Although enduring bonds between adult mates are important for the physical and mental health of individuals and their children, and may also influence societal stability, we know surprisingly little about the neurobiology of pair bonding. This is partly due to the fact that traditional laboratory rodents used in the study of behavioral neuroendocrinology generally do not display behavioral characteristics of a pair bond, and thus cannot be used as model systems for the study of pair bonding. While a variety of nontraditional animal models have emerged to study this rare behavior, including marmoset and titi monkeys [15, 197] and California mice [2426, 59, 189], we will focus on one that has become increasingly popular; the prairie vole (Microtus ochrogaster). We will begin by describing field and laboratory studies that document prairie vole pair bonding behavior. Then we will discuss early work in the laboratory that described the neural correlates of pair bonding behavior in prairie voles. Next, we will discuss the neurobiological mechanisms involved in three separate behaviors associated with pair bonding; the formation of partner preferences, the development of selective aggression toward unfamiliar conspecifics, and the bi-parental care of young—concentrating primarily on paternal care as maternal care is common to all mammalian species and has been extensively reviewed elsewhere [31, 170, 171, 199]. We will focus on the involvement of the neuropeptides arginine vasopressin (AVP) and oxytocin (OT) and the neurotransmitter dopamine (DA) in these behaviors, but will also review other neurochemicals that have been implicated in pair bonding. Finally, we will explore how these neurochemicals may work together to regulate the formation and maintenance of pair bonds.

2. The prairie vole model

2.1. Field studies of behavior

The prairie vole is a socially monogamous rodent species that lives primarily in the grasslands of the central United States [106]. It has been suggested that adaptation to this harsh environment, with limited food sources and scarce water supplies [27, 92, 159], may have contributed to the evolution of a socially monogamous life strategy in this species [38, 218].1 Early field studies using multiple-capture traps offered evidence that prairie voles form long-term bonds and travel together in the wild, as male and female pairs were repeatedly captured together [94]. Further, the use of radiotelemetry combined with repeat-trapping allowed for the observation that male and female pairs co- occupy nests and share home ranges during both breeding and nonbreeding seasons [69, 94, 95]. Additional studies demonstrated that such breeding pairs typically remain together until one member dies, and in many cases, the surviving partner does not pair with a new mate [38, 96, 97]. Further, male prairie voles contribute to nest guarding, by excluding unfamiliar males and females from the vicinity of the nest and home range, and also contribute to nest building [97, 205]. Although male parental behaviors were difficult to observe in natural conditions, due to the findings described above and the high degree of paternal investment found in other monogamous species, it was predicted that male prairie voles were highly paternal [205, 230], and this prediction was confirmed in subsequent behavioral studies under laboratory conditions.

2.2. Laboratory studies of behavior

Prairie vole pair bonding behaviors have been extensively characterized in the laboratory. Sexually naïve prairie voles are highly social and display nonselective affiliative behavior toward conspecifics [194]. Following extended cohabitation and/or mating, prairie voles develop social and sexual preferences for their familiar partner [68, 69, 102, 229]. This selective affiliation (Fig 1A) is accompanied by selective aggression toward unfamiliar conspecifics [8, 99, 100, 124, 223, 224, 231]. Additionally, the mated pair shares a nest, remains together during gestation, and displays bi-parental care throughout lactation [158, 174]. Below, we describe in detail these behaviors and the behavioral paradigms used to measure them.

Figure 1
Laboratory characterization of behaviors associated with pair bonding. (A) Photo illustrates a male and female prairie vole displaying side-by-side contact. (B) Three-chamber apparatus used to test for partner preferences. Three identical cages are connected ...

Partner preference formation is a reliable index of pair bonding, and is characterized by selective contact, affiliation, and copulation with the partner over a stranger [105]. In a controlled environment, this behavior is studied using a three-chamber partner preference test first developed in the laboratory of Dr. Sue Carter [229] and subsequently adopted by many other laboratories. The testing apparatus consists of a central cage that is connected by hollow tubes to two identical cages, one containing a familiar animal (partner) and the other an unfamiliar animal (stranger) (Fig 1B). These two stimulus animals are loosely tethered into their respective cages and are not allowed to interact with one another. During a 3-hr partner preference test, the subject is placed into the central chamber and allowed to move freely throughout the testing apparatus. In some laboratories, a customized computer program—in conjunction with photobeam light sensors placed across the hollow tubes that connect the cages—is used to monitor the amount of time that the subject spends in each cage and the frequency of cage entries. Social behaviors, including mating and side-by-side contact, are videotaped during this test and subsequently quantified. Partner preference formation is inferred when the subject spends significantly more time in side-by-side contact with the partner than with the stranger. In both male and female prairie voles, 24 hours of cohabitation with mating reliably induces partner preference formation, whereas 6 hours of social cohabitation in the absence of mating does not induce this behavior [124, 125, 229] (Fig 1C). This behavioral paradigm has been successfully used in neuroanatomical, neurochemical, and pharmacological studies to examine the neurobiology of partner preference formation [216, 237, 245].

Another behavior that emerges after mating in prairie voles is aggression toward conspecific strangers. This aggression is directed toward unfamiliar males and females, but not the familiar partner, and has therefore been termed ‘selective aggression’. Selective aggression in prairie voles is assessed in the laboratory using a resident-intruder paradigm similar to that used in mice [162, 231]. In this paradigm, an unfamiliar conspecific animal (intruder) is placed into the home cage of the subject (resident). Behavioral interactions between the resident and intruder are videotaped during a 6–10 minute test, and the frequency and duration of a variety of aggressive behaviors are subsequently quantified. Studies using this paradigm have demonstrated that sexually naïve male prairie voles display very low levels of aggression toward intruders [124, 224, 231]. However, after 24 hours of cohabitation with mating, aggressive behaviors toward intruders are dramatically increased [124, 224, 231]. While this aggression is directed at both males and females, intense offensive attack behaviors are only noted toward stranger males, but not stranger females, at this time point [224]. Selective aggression is also enduring; it lasts for at least two weeks after partner preference formation [8, 99, 100]. Further, males paired for this extended period of time (i.e. pair-bonded males), in contrast to those paired for only 24 hours, show intense attack behaviors toward stranger females, even those that are sexually receptive, thereby rejecting potential new mates (Fig 1D) [8, 99, 100]. It has therefore been suggested that selective aggression not only plays an important role to guard mate and territory [37, 38], but may also function to maintain the existing pair bond [8, 10] and to limit extra-pair copulations. Although selective aggression has only been systematically tested in male prairie voles, evidence exists to suggest that females may also display this behavioral pattern [94]. The reliable expression of both partner preference and selective aggression by prairie voles in carefully controlled laboratory conditions highlights the utility of this animal model in behavioral neuroendocrine studies.

Prairie voles, similar to most species that form pair bonds between adult mates [86], display bi-parental care of young (i.e., both the mother and father help to rear offspring) (Fig 1E). As maternal care is ubiquitous across mammalian species, we will focus our discussion of bi-parental care on the role of the father (i.e., paternal care). Paternal behavior in prairie voles has been observed in the laboratory using semi-naturalistic enclosures [104, 158, 174]. After litter birth, fathers display all patterns of parental behaviors exhibited by females, except nursing [174, 205]. These include direct parental behaviors, such as huddling over (i.e., crouching), grooming, contacting, and retrieving pups as well as indirect behaviors, such as nest building and food hoarding [104, 174, 205, 230]. Fathers even continue to display paternal care toward their juvenile offspring after the birth of subsequent litters [218, 220]. However, in the presence of juveniles, prairie vole fathers spend less time in the natal nest displaying paternal behavior and more time foraging [93, 218]. The presence of juveniles may reduce the need for direct paternal care by the father, as juveniles that remain in the natal nest beyond weaning often contribute to the care of subsequent litters—a behavior called ‘alloparenting’ [104, 198, 218, 220, 222]. Alloparental behavior in juvenile and sexually naïve adult male prairie voles qualitatively resembles paternal care in fathers [198, 218, 220], and these paternal behaviors are enhanced by social/sexual experience with a nonrelated female [18]. Importantly, the presence of the father and the display of paternal behavior have been shown to facilitate the physical and behavioral development of offspring [4, 218, 220], a finding similar to the aforementioned beneficial effects of paternal care on our own children. Thus, understanding the mechanisms regulating paternal behaviors could provide important information about optimal parental care in mammalian species that form pair bonds, including our own.

3. Neural correlates of prairie vole pair bonding

Early studies investigating the neural correlates of pair bonding compared neuropeptide and neurotransmitter systems between vole species that displayed disparate life strategies. The four species used were prairie, pine (M. pinetorum), meadow (M. pennsylvanicus) and montane (M. montanus) voles. Monogamous prairie and pine voles form pair bonds between adult mates and show bi-parental care of offspring while promiscuous meadow and montane voles do not form pair bonds and display only maternal care [37, 82, 91, 95, 104, 124, 126, 127, 154, 155, 158, 174, 230]. The close taxonomic relationship shared by these species, coupled with their differences in life strategy make these rodents ideal for comparative studies investigating social behavior (for review, see [237]).

As AVP and OT were known to regulate species-specific social behaviors, including sexual behavior (for review, see [11]), aggression [79], and maternal care [129, 176, 177], it was predicted that these neuropeptide systems would differ between monogamous and promiscuous species [17, 122]. To test this hypothesis, the distribution patterns of AVP and OT cells, fibers, and receptors were mapped in the vole brain. In all vole species examined, regardless of life strategy, AVP-immunoreactive (AVP-ir) neurons were found in several brain regions, including the paraventricular (PVN) and supraoptic (SON) nuclei of the hypothalamus, the bed nucleus of the stria terminalis (BNST), medial amygdala (MeA), anterior hypothalamus (AH), and preoptic area (POA) [17, 221, 223]. AVP-ir fibers were found in the lateral septum (LS), lateral habenular nucleus (LHN), diagonal band (DB), BNST, medial preoptic area (MPOA), and MeA [17, 223]. OT-immunoreactive (OT-ir) cells and fibers were located in several brain areas in each species, including the PVN, SON, MPOA, and BNST [223], and OT-ir fibers were also found in the nucleus accumbens (NAcc) [187]. Although subtle species differences were found, in general, the distribution patterns of AVP-ir and OT-ir neurons and fibers are highly conserved between monogamous and promiscuous vole species [187, 221, 223].

Remarkable species differences are noted, however, in the distribution patterns and regional densities of AVP and OT receptors (OTRs). Prairie voles, for example, have higher densities of AVP-V1a receptors (V1aRs) in the BNST, ventral pallidum (VP), central (CeA) and basolateral (BLA) nuclei of the amygdala, and accessory olfactory bulb (AOB), among other regions, than montane voles, whereas higher densities of V1aRs are noted in the LS and medial prefrontal cortex (mPFC) of montane voles than prairie voles [123, 145, 196, 225, 241] (Fig 2A). Interestingly, when multiple vole species were compared, monogamous prairie and pine voles showed a similar pattern of V1aR binding, and this pattern differed from that of promiscuous meadow and montane voles [123, 145], indicating a potential involvement of region-specific V1aRs in cognitive and behavioral functions associated with different life strategies in voles [123, 145, 196, 241]. Similarly, the distribution patterns and regional densities of OTRs also differ between monogamous and promiscuous vole species. Monogamous prairie and pine voles, for example, have higher OTR densities in the BNST, mPFC, and NAcc than promiscuous meadow and montane voles (Fig 2D), whereas the opposite pattern is found in the levels of OTR binding in the ventromedial hypothalamus (VMH), LS, and anterior cortical amygdala (AcA) [122, 196, 239]. Species differences in V1aR and OTR distribution are stable across the lifespan [215, 225] and are receptor-specific, as no such differences exist in benzodiazepine or opiate receptor systems [122]. Therefore, given the role of AVP and OT in social behaviors, species differences in V1aRs and OTRs are thought to be specifically related to species differences in social behaviors associated with different life strategies in voles [107].

Figure 2
Vasopressin (AVP) and oxytocin (OT) regulation of partner preference formation. (A) Species differences in vasopressin receptor (V1aR) binding in the ventral pallidum (VP) of prairie and montane voles. Higher densities of receptors are indicated by more ...

The drastic species differences in neuropeptide receptor distribution described above may be due to the subtle species differences noted in the promoter regions of the V1aR and OTR [239, 240, 242, 243]. Although the genetic structure of the V1aR and OTR coding regions are strikingly similar across vole species [239, 240, 242, 243], prairie and pine voles carry several repetitive microsatellite DNA sequences in the promoter region of the V1aR gene that are not found in meadow or montane voles, and these sequence changes may underlie species differences in receptor expression [107, 108, 242, 243]. In support of this idea, mice carrying a transgene coding for the prairie vole V1aR, exhibited central V1aR patterns similar to prairie voles [243]. Interestingly, when injected with AVP, these transgenic mice displayed enhanced social affiliation, indicating that receptor distribution patterns may influence brain responsiveness to endogenous neuropeptides and, in this way, may modulate social behaviors [243].

More recently, comparative studies have investigated central DA systems in vole species, because DA, like AVP and OT, plays a well-known role in processes and behaviors associated with pair bonding, including learning and memory [1, 23, 141, 234], olfaction [164], sexual behavior [22, 117], and parental behavior [170, 171]. These studies have noted differences in both DA cell and receptor distribution patterns, as well as differences in their regional densities, between monogamous and promiscuous voles that may be related to social behavior.

Consistent with findings in other rodent species [44, 114, 167, 210], DA cells—those that label for tyrosine hydroxylase (TH; the rate limiting enzyme in catecholamine synthesis) in the absence of DA beta hydroxylase (the enzyme that converts DA to norepinephrine)—have been found in multiple regions in the monogamous prairie vole brain, including the principal nucleus of the BNST (pBNST), posterodorsal MeA (MeApd), and ventral tegmental area (VTA) [99, 168]. Additionally, a high density of DA terminal innervation is present in the NAcc and caudate putamen (CP) [7], and recent tract tracing experiments in males have demonstrated that these terminals arise from projection neurons in the VTA [101], as has been demonstrated in other species [34, 128, 193]. However, promiscuous meadow voles contain very few, if any, DAergic cells in the pBNST and MeApd [168], further demonstrating neuroanatomical differences between monogamous and promiscuous vole species.

Dopamine receptor (DAR) distributions in the vole brain have also been characterized. DARs can be classified into two main families, D1-like (D1R) and D2-like (D2R) receptors, that are differentiated by their molecular structures, pharmacological affinities, and effects on intracellular signaling pathways [163, 166]. In prairie voles, D1Rs are found in the NAcc, CP, and mPFC, as well as other brain regions [8, 196] (B.J. Aragona, Y. Liu, and Z.X. Wang, unpublished data). D2Rs, while present in these same regions, can also be found in the VTA and substantia nigra (SN) [8, 196] (B.J. Aragona, Y. Liu, and Z.X. Wang, unpublished data). Although these receptor distributions are similar to those found in other rodent species, their relative densities are species-specific and may correlate with species differences in social behavior [8, 196]. For example, monogamous prairie voles have higher densities of D2Rs and lower levels of D1Rs in the mPFC than promiscuous meadow voles [196]. Further, meadow voles have a significantly higher density of D1Rs in the NAcc than prairie voles, a finding thought to be related to the relatively low degree of social affiliation noted in meadow voles [8]. Indeed, pharmacological blockade of D1Rs in the NAcc increased affiliative behaviors in meadow voles [8].

Taken together, these studies have demonstrated differences in AVP, OT, and DA systems between vole species with distinct life strategies. As a result, researchers have focused on these systems in the prairie vole brain to systematically examine the neurobiology of behaviors intrinsically associated with pair bonding, including partner preference formation, selective aggression, and paternal behavior. We will discuss the neurobiological regulation of each of these behaviors, in turn, in the following sections.

4. Neurobiology of partner preference formation

4.1 Brain activation associated with partner preference formation

One commonly used approach in the study of interactions between the brain and behavior is to map immediate early gene expression in the brain following a behavioral test. For example, Fos is the protein product of the immediate early gene, fos, that is rapidly expressed in neurons following activation and can be easily visualized by immunocytochemistry. Therefore, Fos immunoreactive (Fos-ir) staining has been used in behavioral neuroendocrine experiments to identify regional neuronal activation in the brain associated with the display of specific behaviors.

In prairie voles, heterosexual pairing, cohabitation, and/or mating induced Fos-ir staining in several brain areas including the MeA, BNST, and MPOA in both males and females [56, 169]. Mating, in particular, was related to increased Fos-ir levels in the MeA, BNST, MPOA, and gracile nucleus of the medulla oblongata, implicating these brain areas as functional components of a mating circuitry that may contribute to partner preference formation [50, 51, 169]. A role for the MeA in prairie vole partner preference has been further implied by lesion studies, as axon-sparing lesions of the MeA in male prairie voles decreased their affiliative behavior toward a familiar female but had no effect on exploratory behavior, locomotion, or olfactory investigation [133].

4.2. Neuropeptide regulation of partner preference formation

The first evidence indicating that AVP and OT may play an important role in partner preference formation came from studies investigating the effects of social and sexual experience—prerequisites for naturally induced partner preference formation—on these neuropeptide systems in the prairie vole brain. In male prairie voles, cohabitation with mating increased the number of AVP mRNA-labeled cells in the BNST [214] and decreased the density of AVP-ir fibers in the LS [18]. As BNST-AVP neurons project to the LS [60], these data suggest that mating facilitates AVP synthesis in the BNST and AVP release in the LS of male prairie voles [216]. Since mating is essential for partner preference formation in males [124], these data offer correlative evidence of the involvement of AVP in partner preference formation. In females, instead, exposure to male chemosensory cues altered OTR density in the AOB, indicating that OT may play a role in partner preference formation in female prairie voles [233].

Direct evidence of a role for AVP and OT in partner preference formation was provided by pharmacological manipulation of these systems. Intracerebroventricular (icv) administration of a V1aR antagonist blocked partner preference formation in male prairie voles, while central AVP administration induced partner preferences in the absence of mating [43, 231]. Similarly, icv administration of AVP induced partner preferences in female prairie voles after just 1 hour of cohabitation with a male, and this effect was blocked by concurrent administration of a V1aR antagonist, indicating that AVP regulates partner preference formation in both sexes [43]. OT treatment also influenced partner preference formation in both sexes. Specifically, icv OT administration induced partner preferences in both males and females and these effects were blocked by concurrent administration with an OTR antagonist [43]. While these data indicate that both AVP and OT regulate partner preference formation in both sexes, it is important to note that the effective doses of neuropeptides differ between males and females [43].

Site-specific manipulations have since demonstrated several brain regions important for the AVP and OT regulation of partner preference formation. In males, administration of a V1aR antagonist directly into the LS or VP, but not several other brain regions, inhibited the formation of mating-induced partner preferences, whereas administration of AVP directly into the LS induced partner preferences in the absence of mating (Fig 2B) [146, 149]. Further, administration of an OTR antagonist into the LS of male prairie voles also prevented mating-induced partner preference formation [149]. In females, instead, the prelimbic cortex (PLC; a part of the mPFC) and the NAcc have been implicated in the neuropeptidergic regulation of partner preference formation [150, 244]. OT levels increased in the NAcc during sociosexual experience with a male [187]. Additionally, OT injection directly into the NAcc induced partner preferences in the absence of mating, while blockade of OTRs in this region or the PLC prevented the formation of mating-induced partner preferences (Fig 2E) [150, 244].

Several studies utilizing viral vector-mediated gene transfer to deliver and regulate the expression of genes of interest to specific brain regions have supported the findings that AVP neurotransmission in the VP and OT neurotransmission in the NAcc regulate partner preferences in male and female prairie voles, respectively. In males, for example, an adeno-associated viral vector was used to deliver the V1aR gene into the VP [183]. As expected, this manipulation resulted in an increased density of V1aRs in this region. Interestingly, these males formed partner preferences in the absence of mating, supporting the findings that enhanced AVP neurotransmission in the VP can facilitate partner preference formation in male prairie voles [183] (Fig 2C). Further, V1aR overexpression in the VP of male meadow voles, induced partner preference formation in this socially promiscuous species [145]. Similarly, OTR overexpression in the NAcc of sexually naïve female prairie voles accelerated partner preference formation as compared to controls (Fig 2F), but this treatment did not alter partner preference formation in female meadow voles [188]. Taken together, these studies highlight the importance and site-specific effects of AVP and OT on partner preference formation in male and female prairie voles.

4.3. DA regulation of partner preference formation

Recent work has demonstrated that partner preference formation in prairie voles is also regulated by central DA, particularly the mesolimbic DA system—a group of DA producing cells that originate in the VTA and project to the NAcc, mPFC, and other forebrain regions. This neural circuit is thought to be integrally involved in the assignment of motivational value to environmental stimuli, resulting in the generation of adaptive goal-directed behaviors [120, 232]. For example, mesolimbic DA has long been implicated in assigning salience to incentives such as food and receptive mates, thereby mediating behaviors such as feeding and reproduction that are essential for survival [120, 232]. Similarly, mesolimbic DA has been proposed to facilitate mate choice, enabling mating effort to be focused on preferred conspecifics [80], a hypothesis supported by the data described below. The involvement of this system in partner preference formation makes sense in an evolutionary context, as selection pressures that necessitate the formation of a partnership between mates would likely lead to an increased motivational value assigned to one’s partner, and the selective affiliation that is characteristic of a pair bond.

Early experimental evidence suggesting the involvement of DA in partner preference formation came from peripheral pharmacological manipulations. Recall that 24 hours of cohabitation with mating reliably induces partner preferences in male and female prairie voles. While partner preference formation was not affected by saline injection prior to pairing, treatment with the nonselective DAR antagonist, haloperidol, blocked mating-induced partner preferences in both sexes [7, 217]. Further, treatment with low doses of apomorphine, a nonselective DAR agonist, facilitated the formation of partner preferences after only 6 hours of cohabitation in the absence of mating [7, 217]. Taken together, these findings suggest that DAR activation is essential for partner preference formation in prairie voles.

The first functional evidence to implicate mesolimbic DA in partner preference formation was the finding that mating increases DA activity in the NAcc of both male and female prairie voles [7, 98]. In females, for example, extracellular DA levels increased nearly 51% above baseline during mating [98]. Similarly, mated males had 33% more DA turnover in this region compared to non-mated males [7]. Direct evidence for the role of NAcc DA in partner preference formation came from site-specific pharmacological manipulations of DA neurotransmission. Microinjection of haloperidol into the NAcc prevented the formation of mating-induced partner preferences, while microinjection of apomorphine into this region facilitated partner preference formation in the absence of mating [7]. These effects were site-specific, as DAR manipulation in the CP, a region adjacent to the NAcc that also receives DAergic innervation from midbrain regions, did not alter partner preference formation [7].

Additional experiments used receptor specific agonists/antagonists to demonstrate that D1Rs and D2Rs in the NAcc differentially regulate partner preference formation (Fig 3A&B). Specifically, NAcc D2R activation facilitated, and D2R blockade prevented, partner preference formation in both male and female prairie voles, indicating that NAcc D2R activation is both necessary and sufficient for partner preference formation [8, 98]. In contrast, NAcc D1R activation prevented mating- and D2R agonist-induced partner preference formation in male prairie voles, indicating an inhibitory role of NAcc D1Rs on this behavior [8]. Importantly, these manipulations were only effective when delivered into the NAcc shell, but not the core, indicating a subregional regulation of partner preferences within the NAcc [8].

Figure 3
Dopamine (DA) in the nucleus accumbens (NAcc) regulates partner preference formation in prairie voles. (A) Cartoon illustrates the mesolimbic DA circuit. DA-ergic cells in the ventral tegmental area (VTA) project to the NAcc and prefrontal cortex (PFC), ...

The DAR-specific regulation of partner preference formation in the NAcc has recently been examined on an intracellular level. D2Rs and D1Rs are both 7-transmembrane receptors whose intracellular effects are mediated by heterotrimeric GTP-binding proteins (G-proteins) (for reviews, see [163, 166]). While D2Rs and D1Rs have similar effects on some signaling pathways, they differentially regulate the intracellular cyclic adenosine 3′, 5′-monophosphate (cAMP) signaling cascade through the alpha subunit of the G-proteins with which they interact [163, 166](Fig 3C). D2Rs bind to inhibitory G-proteins (Gαi and Gαo). When D2Rs are activated, the alpha subunit of Gαi/o inhibits adenylate cyclase (AC) activity, leading to the inhibition of cAMP production and a decrease in the activity of protein kinase A (PKA) [163, 166]. D1Rs, instead, bind to stimulatory G-proteins (Gαs and Gαolf). D1R activation leads to an increase in AC activity, cAMP production and PKA activation [163, 166]. As D1R and D2R activation differentially affect cAMP signaling, it has been suggested that this signaling pathway may underlie the DAR-specific regulation of partner preference formation [9]. In support of this hypothesis, reduction of PKA activity within the NAcc shell, but not core, facilitated partner preference formation in male prairie voles, a result consistent with the effects of D2R activation [8, 9] (Fig 3D). Further, in two separate experiments, activation of stimulatory G-proteins and activation of PKA in the NAcc shell each prevented the formation of mating-induced partner preferences, consistent with the effects of D1R activation [8, 9] (Fig 3D). Importantly, these manipulations did not alter mating or the duration of contact during the 24 hours of pairing, suggesting that increased cAMP signaling directly interferes with partner preference formation. Taken together, these experiments demonstrate that cAMP intracellular signaling in the NAcc shell regulates partner preference formation, and may underlie the DAR-specific effects on this behavior.

5. Neurobiology of selective aggression

As previously mentioned, after 24 hours of mating and the formation of partner preferences, male prairie voles display high levels of aggression toward conspecific strangers, particularly male strangers, but not toward their partners [124, 224, 231]. Additionally, after one to two weeks of extended cohabitation and mating with their partner, pair-bonded male prairie voles display intense aggression toward both male and female intruders, including sexually receptive females, thereby rejecting potential new mates [8, 99, 100, 231]. This selective aggression is thought to be essential for mate guarding, nest defense, and the maintenance of the existing bond between the male and his partner [8, 37, 38, 99, 100, 231]. As described below, studies have indicated multiple brain regions, and the involvement of both AVP and DA in this behavior.

5.1. Brain activation associated with selective aggression

A variety of brain regions have been implicated in selective aggression. For example, display of this behavior has been associated with elevated Fos-ir in the MeA, BNST, MPOA, LS, and AH (Fig 4A) [99, 224]. In one of these regions, the AH, differential activation was noted between exposure to the familiar partner and an unfamiliar stranger [99]. Specifically, male prairie voles that were paired with a female for two weeks (i.e., pair-bonded) and were then exposed to a conspecific male or female stranger had a significantly higher density of Fos-ir cells in the AH than pair-bonded males re-exposed to their partner. Interestingly, males exposed to either male or female intruders also had a significantly higher density of cells double-labeled for AVP-ir and Fos-ir in this brain region than males re-exposed to their partners, suggesting that AH AVP may regulate selective aggression [99] (Fig 4B).

Figure 4
Vasopressin (AVP) and dopamine (DA) involvement in selective aggression in male prairie voles. (A) Photomicrograph shows AVP-immunoreactive (AVP-ir) cell bodies and fibers (brown cytoplasmic staining), Fos-immunoreactive (Fos-ir) staining (dark nuclear ...

5.2. Neuropeptide regulation of selective aggression

Due to the known role of AVP in territorial displays [79], and the differences in AVP receptor distribution in forebrain regions between monogamous and polygamous voles [123, 225], AVP was hypothesized to be involved in the regulation of selective aggression. In the first experiment to test this hypothesis, Winslow et al. (1993) found that injection of a V1aR antagonist, but not cerebrospinal fluid (CSF), into the lateral ventricle during 24 hours of mating prevented the subsequent display of mating-induced selective aggression in male prairie voles. Additionally, infusion of AVP into the lateral ventricles induced aggression toward an intruder in sexually naïve, non-female exposed males. Similar manipulations of the OT system did not alter aggressive behaviors, indicating that central AVP, but not OT, neurotransmission regulates selective aggression in male prairie voles [231].

Site-specific manipulations in the AH have further supported this hypothesis [100]. Sexually naïve males that received AVP infusion directly into the AH showed significantly higher levels of aggression toward a novel female than males treated with vehicle or with both AVP and a V1aR antagonist, indicating that AVP neurotransmission in the AH can induce aggression in prairie voles (Fig 4E). Further, in pair-bonded male prairie voles, AVP release in the AH was significantly higher in subjects exposed to a stranger animal than those exposed to their partners (Fig 4C). Interestingly, the magnitude of AVP release in these animals was correlated positively with their frequency of aggression and negatively with the duration of affiliation. Additionally, blockade of V1aRs in the AH, but not other brain regions, prevented the display of selective aggression in pair-bonded males, directly implicating AH AVP in this behavior (Fig 4E). In the same study, it was found that pair-bonded males had significantly higher densities of V1aRs, but not OTRs, in the AH than sexually naïve males (Fig 4D), suggesting that pair bonding experience may cause neuroplastic changes in the AH AVP system that underlie the emergence of selective aggression [100]. This hypothesis was supported by the finding that artificial overexpression of the V1aR by viral vector-mediated gene transfer, in sexually naïve prairie voles enhanced aggression toward novel females (Fig 4F)[100]. Taken together, these data indicate that AVP in the AH plays an integral role in the regulation of selective aggression in male prairie voles.

5.3. DA regulation of selective aggression

Mesolimbic DA has also been implicated in selective aggression, particularly, the aggression displayed by pair-bonded males toward stranger females [8]. In two separate experiments, DAR densities in the brains of male prairie voles that were sexually naïve were compared to that of males either paired with a female for 24 hours or two weeks (i.e. pair-bonded) [8]. Although no differences in DAR densities were noted between sexually naïve males and those that had mated with a female for 24 hours, pair-bonded males had significantly higher levels of D1Rs, but not D2Rs, in the NAcc, but not CP, than their sexually naïve counterparts (Fig 4G and H). As two weeks, but not 24 hours, of cohabitation and mating increased NAcc D1Rs, these results indicate that this neuroplastic change is not necessary for the initial formation of partner preferences—a result that is consistent with the D2R, but not D1R, regulation of partner preference formation aforementioned—but is instead indicative of extended sociosexual experience with the partner (i.e. the full establishment of a pair bond) [8]. Interestingly, this increase in D1R levels in pair-bonded males coincides with the behavioral emergence of offensive aggression toward a stranger female (males that are paired with a female for two weeks display robust offensive aggression toward stranger females [8, 99, 100], whereas males that are sexually naïve or allowed to mate with a female for 24 hours do not [224]). Therefore, it was hypothesized that increased D1R levels in the NAcc of pair-bonded males may regulate selective aggression toward stranger females. Site-specific pharmacological blockade of NAcc D1Rs was used to test this hypothesis. While pair-bonded males treated with CSF displayed robust offensive aggression toward a female intruder, intra-NAcc injection of a D1R antagonist abolished this aggression (Fig 4I). Taken together, these data suggest that NAcc D1R upregulation may underlie the important behavioral transition that occurs in male prairie voles as they progress from the state of being sexually naïve to being fully pair-bonded, leading to offensive aggression toward stranger females and the maintenance of the established pair bond [8]. In an interesting parallel to this finding, repeated exposure to a common drug of abuse, amphetamine, increased aggression toward conspecifics and prevented the formation of partner preferences [100, 151]. Importantly, these behavioral changes coincided with an upregulation of D1Rs in the NAcc and V1aRs in the AH, indicating that drugs of abuse can hijack natural forms of neuroplasticity that evolved to maintain pair bonds [100, 151].

6. Neurobiology of Paternal Behavior

Paternal behavior has been reported in several nonhuman monogamous mammalian species including tamarins [246], marmosets [5], titis [160, 161], hamsters [118], gerbils [182], mice [24] and voles [174, 205]. Studies in nonhuman primates have focused on the characterization of paternal behaviors and the effects that manipulations of the social environment have on the display of these behaviors, and have provided important translational information for human health. Studies in rodents, instead, have focused on the central regulation of paternal behaviors and have provided valuable information concerning the neural mechanisms underlying paternal behavior. Although the California mouse has proven a useful rodent model for this purpose [2426, 59], the vole model has perhaps been used most extensively in studies of the neurobiology of paternal behavior, and data from these studies are summarized below.

6.1. Brain activation associated with paternal behavior

As in the study of partner preference formation and selective aggression, early studies examining vole paternal behavior used Fos-ir to map brain areas that are activated by pup exposure and the display of paternal behavior. After exposure to a conspecific pup, male prairie voles displayed increased Fos-ir staining in some forebrain areas including the AOB, MeA, BNST, MPOA, and LS, implicating the involvement of these regions in processing pup-associated cues and/or in the regulation of paternal behavior [134, 222]. The role of the olfactory system and MeA in paternal behavior was further confirmed by lesion studies in prairie voles. Males that received bilateral bulbectomy showed a significant decrease in paternal behavior along with other social behaviors, compared to males that received a sham-operation [135]. Further, axon-sparing lesions of the MeA decreased paternal behavior in male prairie voles without affecting other behaviors such as exploration, locomotion, and olfactory investigation [133]. Finally, unlike prairie voles, pup exposure did not significantly elevate Fos-ir labeling in the MeA, BNST, MPOA, or LS of male meadow voles, further suggesting the importance of these brain regions in the regulation of male parental care [222].

6.2. Neuropeptide regulation of paternal behavior

In addition to those behaviors previously mentioned, central AVP and OT have also been implicated in parental behaviors, particularly in females. Injections of AVP into the lateral ventricle of female rats induces persistent parental behavior [177]. Further, Long-Evans rats display superior parental behavior compared to their closely related AVP-deficient mutant variants, Brattleboro rats [227]. OT, both in the periphery and brain, also plays an important role in behaviors associated with maternal care, including uterine contraction at parturition, milk ejection during lactation [87, 211] and the regulation of maternal behavior in females [142, 177]. As OT and AVP have therefore been implicated in parental behavior in females, and other social behaviors in both females and males, researchers began evaluating the role that these neuropeptides played in the regulation of male parental behavior.

The first evidence to suggest a role for these neuropeptides in paternal behavior was provided by studies investigating the relationship between paternal experience and AVP-ir fiber density or AVP/OT mRNA expression in the brain. Prairie voles that had been paired with a female for two weeks or were first-time fathers demonstrated significantly more paternal behaviors and had lower densities of AVP-ir fibers in the LS, but not the MPOA, than their sexually naïve counterparts [17, 18]. Interestingly, this alteration in LS AVP-ir fiber density was not found in meadow vole fathers—that naturally show little to no paternal behaviors toward pups—suggesting that changes in LS AVP may indeed play a role in prairie vole paternal behavior [17]. AVP in the PVN has also been implicated in prairie vole paternal behaviors, as AVP mRNA labeling in this region was increased in male prairie voles that had recently become fathers, but did not change in naturally non-paternal male montane vole fathers [226]. Although little is known about the role of OT in paternal behavior in prairie voles, there exists some evidence that this neuropeptide may be involved. For example, prairie vole pups reared by mothers-only received less licking/grooming and matured more slowly, compared to pups reared by both parents. In adulthood, the former showed less pup-directed parental behavior and increased OT mRNA expression in the hypothalamus than the latter, but such effects were mainly noted in females [4]. Taken together, these data indicate that AVP and OT in various brain regions may regulate paternal behavior.

Few studies have directly assessed the functional significance of central AVP and OT in paternal behavior. In one of these studies, subtle changes were noted in the paternal behaviors of sexually naïve males after icv administration of AVP or OT, whereas combined icv treatment of an OTR and V1aR antagonist affected paternal behaviors in a dose-dependent manner [13]. At low doses (1ng each), OTR/V1aR antagonists tended to increase the latency for pup approach and huddling, while at high doses (10ng each) paternal behavior was significantly reduced and the occurrence of pup attacks was significantly increased [13]. While this study demonstrates that central AVP and OT indeed have functional effects on paternal behavior, further experimentation is required to further understand the role of each neuropeptide on specific paternal behaviors and their sites of action within the brain. In the only study to do so to date, Wang et al (1994) examined the effects of AVP manipulation in the LS on four of the most common paternal behaviors, including licking/grooming, crouching/huddling over, contacting and retrieving pups. Sexually naïve male prairie voles injected with AVP directly into the LS spent significantly more time displaying paternal activities, specifically contacting and crouching over pups, than voles injected with saline. These effects were blocked by pre-injection of a V1aR antagonist in the LS, suggesting that LS AVP is both necessary and sufficient in the regulation of paternal behavior [213].

Although site-specific effects of OT on paternal behavior have never been tested, there is evidence to suggest that NAcc OT may be involved. This evidence stems from comparative studies demonstrating species differences in NAcc OTR densities that correlate with species differences in paternal behavior [122], the importance of OTR activation in other behaviors associated with pair bonding in males (e.g., partner preference formation) [150, 244], and various studies documenting a role of NAcc OTRs in female parental behavior. For example, NAcc OTR densities have been related to spontaneous maternal behavior in sexually naïve adult female prairie voles. Specifically, females that displayed maternal behavior had higher densities of OTRs in the NAcc than females that did not display maternal behaviors or attacked pups [173]. A similar positive correlation was noted between NAcc OTR density and alloparental care in juvenile female prairie voles [172]. Further, OTR density in the NAcc has been positively associated with other affiliative behaviors, including partner preference formation [188]. Although never directly tested in males, these data indicate the potential for NAcc OT to be involved in paternal behavior.

6.3. DA regulation of paternal behavior

While a great deal of research has documented the importance of central DA in maternal behaviors (see [171] for review), fewer studies have investigated a role for central DA in paternal behavior. Although limited in number, these studies have provided compelling preliminary evidence that DA is also involved in male parental care.

In the only pharmacological experiment to examine the DAergic regulation of paternal behavior in prairie voles, Lonstein (2002) illustrated that DAR blockade has differential effects on distinct aspects of paternal behavior (e.g. contacting, licking, and huddling over pups). Specifically, blockade of DARs with the nonselective DAR antagonist, haloperidol, impaired some paternal behaviors—including contacting and licking pups—yet enhanced others, such as huddling over pups. Although haloperidol disrupts general motor activity at some doses [195], the effects of haloperidol on some paternal behaviors, specifically pup licking, were noted at doses that did not alter total activity scores, indicating that DAR activation has primary effects on paternal behavior. Therefore, these data not only demonstrate a role for DA in paternal behavior but also illustrate that the DAergic regulation of paternal behavior is behavior-specific [153]. No site-specific manipulations have yet been used to reveal brain regions involved in the DAergic regulation of paternal behavior. However, an experiment mapping neuronal activation in response to pups has offered some insight into this matter. Recall that the prairie vole brain contains a group of DAergic cells in the pBNST and MeApd that is sexually dimorphic—males have more DAergic cells in these areas than females [168]—and these cells are potentially sensitive to androgens and estrogens [40]. Interestingly, these cell populations are activated (indicated by Fos/TH double-labeling) in the male prairie vole brain after interactions with conspecific pups [168], and may therefore be involved in paternal behavior.

Although no studies have yet been conducted to this end, it is suggested that NAcc DA may also play a role in paternal behavior. As described previously, the vole NAcc contains dense DA terminals and receptors and NAcc DA plays an important role in the regulation of other social behaviors associated with pair bonding, including partner preference formation and selective aggression in male prairie voles [7, 8]. Additionally, NAcc DA plays a well-known role in maternal behavior in other rodent species. In rats, for example, DA is released in the NAcc in response to pup stimuli [109] and alterations in NAcc DA activity across postpartum periods are correlated with changes in a variety of parental behaviors ranging from pup retrieval, nursing, licking/grooming, and maternal memory [3]. It may therefore be worthwhile for future investigations of male parental behavior to examine the possibility that NAcc DA plays an important role.

7. Other neurochemicals/hormones implicated in pair bonding

In addition to AVP, OT and DA, several other neurotransmitters and hormones have been implicated in social behaviors associated with pair bonding in prairie voles. One interesting example involves neurochemicals associated with the hypothalamic-pituitary-adrenal (HPA) axis, the system that mediates stress responses. Briefly, during a stressor, corticotrophin-releasing factor (CRF) released from the hypothalamus binds to CRF receptors in the anterior pituitary leading to the synthesis of adrenocorticotrophic hormone (ACTH) [143]. ACTH is then released into the bloodstream and acts on the adrenal cortex to produce glucocorticoids, such as corticosterone (CORT), which can then act on glucocorticoid receptors (GR) in the brain to mediate responses to stress [143]. Prairie voles are considered to be glucocorticoid resistant rodents as they have about 5- to 10-fold greater basal plasma CORT and 3-fold greater basal levels of ACTH, along with 10-fold lower affinity GRs, especially the type-I GR, that are expressed in lower densities in the brain, compared to rats and promiscuous voles [110, 204].

Data from behavioral experiments indicate that the effect of CORT on pair bonding is sexually dimorphic. In female prairie voles, cohabitation with a male, which led to partner preference formation, significantly decreased serum CORT levels [63]. Further, reduction in GR activity, either by decreasing circulating CORT through adrenalectomy [63] or by treating animals with a GR antagonist [52], facilitated partner preference formation. In contrast, CORT injections or a stressful swim test, which increased circulating CORT [66], prevented the development of partner preference formation [63]. Together, these data suggest that a decrease in HPA axis activity facilitates partner preference formation in female prairie voles. In males, on the other hand, adrenalectomy inhibited partner preference formation and this effect was reversed by CORT replacement [64], indicating that CORT is necessary for partner preference formation in males. Additionally, in a more recent study, loss of a bonded partner significantly increased circulating CORT levels and adrenal gland weight in male prairie voles, suggesting that HPA axis activity may mediate the aversive effects of partner separation and thus, play a role in the preservation and maintenance of existing pair bonds [29]. The HPA axis has also been implicated in paternal behavior. Males exposed to a swimming stress spent significantly more time huddling over pups and a trend toward more time licking and grooming pups than unstressed controls [14]. These behavioral effects were not found in female prairie voles, indicating that the effects of stress on parental behavior—like partner preference formation—may be sexually dimorphic [14].

CRF has also been implicated in pair bonding behaviors. Male prairie voles that received CRF injections displayed partner preferences in the absence of mating, and this induced behavior was blocked by co-administration of a CRF receptor antagonist [67]. Brain areas involved in the CRF mediation of partner preferences have also been identified. Local CRF injections into the NAcc facilitated, whereas CRF receptor antagonists inhibited, partner preference formation in male prairie voles [148]. Further, pairing with a female elicited an increase in CRF mRNA in the BNST of male prairie voles [29]. Finally, icv administration of urocortin-II, a member of the CRF peptide family, increased passive parental behavior in both male and female prairie voles, but this treatment had no effects on anxiety or locomotor behaviors [190].

Several other neurochemicals are also involved in social bonding in prairie voles. For example, in male prairie voles, intra-VTA administration of NBQX, an AMPA receptor antagonist, or bicuculline, a GABA receptor antagonist, induced partner preference formation, implicating these amino acids in selective affiliation [53]. Administration of the selective serotonin reuptake inhibitor, fluoxetine, increased the latency to engage in parental behavior in both male and female prairie voles, decreased aggression in males, and had no effects on nonsocial behaviors [209], indicating that serotonin may also mediate social behaviors associated with pair bonding. Gonadal steroids can also be added to this list. Manipulation of testosterone or estrogen during the first or second week of life significantly altered subsequent affiliative and/or alloparental behaviors in juvenile prairie voles [138, 184]. Estrogen receptor alpha (ERα) may mediate some of the effects of gonadal steroids on pair bonding behaviors in prairie voles. Studies have demonstrated that females have more ERα-ir cells in several brain areas including the MeA, BNST, MPOA, and VMH than males, and a decrease in ERα-ir staining in the BNST, MPOA, and VMH of females was associated with induction of sexual receptivity [113]. In males, enhanced ERα expression in the MeA, by transfection of an adenoassociated viral vector, disrupted the display of alloparental behavior and impaired partner preference formation [58]. Similarly, males with enhanced ERα expression in the BNST displayed decreased social affiliation [140]. These data indicate an inverse relationship between regional ERα expression and social behavior in prairie voles. Taken together, the studies described above highlight the involvement of multiple neurochemicals and hormones in the regulation of pair bonding behaviors in both male and female prairie voles.

8. Neurochemical/hormone interactions

As reviewed above, a variety of neurochemical, neurotransmitter, and hormone systems have been implicated in pair bonding. However, it is unlikely that these systems act independently to regulate this complex social behavior. In the following section, we will review studies documenting known interactions between some of these systems, including CRF, OT, AVP, DA, glutamate (GLU), gamma-aminobutyric acid (GABA), and gonadal steroid hormones, in the regulation of pair bonding behaviors, primarily partner preference formation.

Two of the first neurochemicals suggested to interact with one another in the regulation of partner preference formation in prairie voles were CORT and OT. Recall that in sexually-naïve females, exposure to an unfamiliar male significantly increased central OT release [187] and decreased serum CORT levels [63], effects thought to facilitate partner preference formation. Interestingly, icv injections of OT produced a comparable decrease in serum CORT levels, suggesting that OT may interact with the HPA axis to regulate partner preference formation [65] — an idea that may warrant future investigation given the suggested interaction between OT and CORT in other social behaviors [6, 39, 42, 132].

OT has also been shown to interact with AVP in the regulation of partner preferences, a finding that is not surprising given that these two neuropeptides are closely related to one another and not only share similar chemical structures—differing by only two amino acids—but can also interact with each other’s receptors [19]. As previously described, icv injection of AVP or OT can induce partner preferences in both male and female prairie voles after as little as 1 hour of cohabitation with an opposite sex conspecific animal. Interestingly, the effects of AVP on partner preference formation are abolished in the presence of an OTR antagonist, and the effects of OT are abolished in the presence on an V1aR antagonist, indicating that AVP and OT can interact to mediate partner preferences [43]. Further, these results indicate that the facilitation of partner preference formation may require simultaneous activation of both V1aR and OTRs [43]. Site-specific manipulation in the LS of male prairie voles has since supported this hypothesis. Partner preference formation induced by AVP microinjection into the LS was blocked by simultaneous administration of an OT receptor antagonist [149]. Taken together, these studies suggest that central OT and AVP systems may work in concert with one another to mediate partner preference formation.

OT and AVP have also been shown to interact with other neurotransmitter systems, such as DA, to mediate partner preferences. In female prairie voles, for example, partner preferences induced by NAcc D2R activation were prevented by concurrent administration of an OTR antagonist [150]. Conversely, partner preferences induced by central OT administration were blocked by concurrent administration of a D2R antagonist in the NAcc [150]. These results suggest that simultaneous activation of both D2Rs and OTRs in the NAcc are required for the facilitation of partner preferences in female prairie voles. AVP-DA interactions have also been implicated in partner preference formation. In a recent study, naturally promiscuous male meadow voles—that would not otherwise form partner preferences with a mate—received viral vector-mediated transfer of the prairie vole V1aR gene into the VP, resulting in upregulation of the V1aR in this region and the formation of partner preferences after 24 hours of mating [145]. In a second experiment, these viral vector-induced preferences were blocked by administration of a D2R antagonist prior to mating, suggesting that AVP and DA may interact to mediate pair bond formation [145]. This hypothesis is supported by the well-known neuroanatomical connection between these two regions, as D2R expressing medium spiny neurons in the NAcc project directly to the VP [90].

GLU, GABA, and CRF have also been suggested to interact with DA in the regulation of partner preferences [52, 53]. Blockade of AMPA GLU or GABA receptors, via injection of NBQX or bicuculline, respectively, into the VTA induced partner preferences in the absence of mating. As the VTA provides the major source of DAergic afferents to mesolimbic brain regions, including the NAcc, it has been suggested that the effects of these antagonists on partner preference formation may have been mediated by their effects on NAcc DAergic neurotransmission [53]. In a separate study, peripheral administration of RU-486, a GR antagonist, induced partner preferences in female prairie voles in the absence of mating [52]. These effects were blocked by coadministration of either a D1R or D2R antagonist into the lateral ventricle, suggesting that the effects of GR antagonism on partner preference formation may be mediated through an interaction with central DA systems [52]. Further experimentation is needed to detail the nature of interactions between GLU, GABA, CRF, and DA in partner preference formation.

Finally, gonadal steroids play an important role in pair bonding and are thought to interact with a variety of neuropeptide and neurochemical systems implicated in this behavior. For example, extended exposure to a male (or male chemosensory signals) increases circulating estradiol levels and subsequently, behavioral estrus, or sexual receptivity in female prairie voles [35, 36, 47, 203]. Sexual receptivity can also be induced in ovariectomized females through estrogen administration alone [35]. Interestingly, increased serum estrogen levels, induced by exposure to male chemosensory signals or by exogenous estrogen administration, significantly increased OTR binding in the prairie vole brain [233], indicating that estrogen and OT may interact to regulate mating—which facilitates partner preference formation in females [228]. In males, testosterone has been found to influence the effects of AVP on partner preference formation. Recall, that icv administration of AVP facilitates the formation of partner preferences in male prairie voles following only 1 hour of cohabitation [43]. Interestingly, AVP administration does not induce partner preferences in adult male prairie voles that were castrated on the day of their birth, suggesting that the organizational effects of testosterone are required for the effects of AVP on partner preference formation [57]. Testosterone and AVP may interact in the regulation of paternal behavior as well, as male prairie voles that were castrated in adulthood had a reduced density of AVP-ir fibers in the LS and displayed less paternal behavior than controls with intact gonads [219] (cf. [152, 153]).

9. Summary and additional considerations

Pair bonding in prairie voles is a complex social behavior that involves the coordination of several distinct behaviors, including selective affiliation, selective aggression, and parental care. The appropriate expression of these behaviors requires a variety of cognitive functions, including sensory processing, memory formation, and individual recognition, as well as motor output. Multiple brain regions, such as the MeA, BNST, LS, NAcc, PFC, and AH, known to mediate these processes, have been implicated in pair bonding behaviors in prairie voles, as described above. Further, many of the neurochemicals that have been implicated in pair bonding behaviors, including AVP, OT, DA, CRF, and GLU are known to act in these brain regions to mediate processes essential for pair bonding. For example, AVP in the LS [78] and OT in the MeA [77] mediate social recognition in other species, a process inherent to the formation and display of partner preferences in prairie voles. As another example, DA in the NAcc plays an important role in conditioned reward learning [23, 119], a process that likely contributes to partner preference formation by mediating a learned association between the reinforcing properties of mating and the specific olfactory signature of the mate [8, 10, 245].

Although careful experimentation has revealed the importance of each of these separate brain regions and neurochemicals for pair bonding behaviors, each behavior tested is undoubtedly regulated by a larger circuitry involving multiple brain regions and neurochemicals. While not yet fully examined in voles, data from other rodent species have demonstrated clear anatomical connections between the brain regions noted above, and these connections form neural circuits with discrete functions relevant to pair bonding [245]. For example, connections between the vomeronasal organ (VNO), AOB, and MeA play an important role in processing chemosensory cues [30, 72, 121, 130, 192], and not surprisingly, activation of both the VNO and MeA during social and sexual experience (when discrete conspecific olfactory cues are present) are essential for the formation of partner preferences [49, 133]. Connections between the MeA and AH regulate aggression [33, 202], and activation of this pathway likely regulates selective aggression in prairie voles [99, 224]. Additionally, the well-characterized mesolimbic DA system, which consists of DAergic cells in the VTA that project to the NAcc and mPFC, is regulated by reciprocal GLUergic projections from the mPFC to the NAcc and VTA [34, 193]. This neural circuit assigns motivational salience to environmentally relevant stimuli [120, 232], facilitating adaptive goal-directed behaviors, such as copulation with a receptive mate [22, 74, 179, 180] and the retrieval of pups [2, 112, 171]. It is important to note that just as the same system can mediate more than one behavior, as evidenced by the latter example, the same neurochemical can regulate more than one behavior. For instance, AVP neurotransmission, in the LS, VP, and/or AH plays an important role in partner preference formation, selective aggression, and paternal behavior. Further, one behavioral event can be mediated by more than one neural circuit. For example, sociosexual experience – a prerequisite for partner preference formation – induces both DA and OT release in the NAcc [98, 187], AVP release in both the LS [216] and VP [245], and has also been suggested to release OT in the PFC [245]. Thus, multiple neural circuits and neurochemicals work in concert with one another to regulate behaviors associated with pair bonding.

Finally, in the discussion of pair bonding in the prairie vole, sex differences should not be overlooked. As discussed above, the neural regulation of pair bonding behaviors is in some cases sexually dimorphic (e.g., CORT regulation of partner preference formation [63, 64]). Further, some neuropeptides, such as AVP and OT, may have gender specific roles in certain behaviors related to pair bonding: AVP regulates selective aggression [99, 100] and paternal behavior [149, 213] in males, while OT regulates maternal care in females [16, 76, 165, 173, 178]. Additionally, males and females seem to differ in their sensitivities to AVP and OT. Although both neuropeptides have been implicated in partner preference formation in both sexes [43], lower doses of AVP were sufficient to induce partner preferences in males than females and peripheral OT administration was effective to induce partner preference formation only in female, but not male, prairie voles [54]. Sexual dimorphisms in physiology and neural substrates may underlie these differences in behavior. For example, as found in other rodent species [48, 61, 207], male voles have more AVP mRNA labeled cells in the BNST and MeA and a higher density of AVP-ir fibers in the LS (most likely projections from AVP producing cells in the BNST and MeA) than female voles [17, 221]. Interestingly, 3 days of cohabitation with an opposite sex conspecific induces an increase in the number of AVP mRNA labeled cells in BNST and a decrease in the density of AVP-ir staining in the LS in male, but not female, prairie voles, indicating a sexually-dimorphic effect of sociosexual experience on AVP activity, which, in turn, may play a role in the regulation of pair bonding behavior in male prairie voles. Sex differences have also been found in a population of TH synthesizing cells in the anteroventral periventricular preoptic area [139] and the extended amygdala [168] in the prairie vole brain, however the functional role of these cells in pair bonding still needs to be examined. It is well recognized that sexual dimorphisms in physiology and neural substrates may underlie sex differences in behavior. In addition, sex differences in the brain may allow for the display of similar behaviors between males and females despite their different physiologies [62]. In other words, sexually dimorphic neurochemical systems may allow males and females to have compensatory mechanisms that work in concert with their physiologies to produce similar behavioral outcomes. This suggestion is consistent with studies in the prairie vole demonstrating that sexually dimorphic systems, such as the AVP pathways from the MeA and BNST to the LS [17], enable the display of parental behaviors in males [216] while OT systems enable the same behaviors in females [16, 173].

10. Conclusions and future directions

Although study of the bonds formed between prairie vole pairs cannot possibly allow us to fully understand the intricacies of human relationships, they can certainly offer insights into the basic neural mechanisms underlying adult attraction and attachment. The literature reviewed above has implicated a variety of neuropeptide, neurotransmitter, and hormonal systems in the regulation of pair bonding in prairie voles. Accordingly, preliminary work in humans has implicated many of these same systems in human social behaviors. For example, recent research utilizing functional magnetic resonance imaging to measure real-time brain activation in humans has suggested that DA neurotransmission may underlie human mate choice and attachment [81]. These studies found that DA-rich areas, such as the VTA, were activated when participants in either early stages of intense romantic relationships or in long-term deeply-loving relationships viewed a photograph of their beloved but not when they viewed pictures of other familiar individuals [12, 20, 21]. Recent studies have also implicated the OT system in human couple interactions. In a placebo controlled experiment, for example, intranasal administration of OT—a method of delivery that readily allows the neuropeptide access to the brain—significantly increased positive communication between couples, as indexed by eye-contact, curiosity/care, and agreement scores [70]. Additionally, OT has been found to increase trust among humans—a prerequisite to social affiliation [137]. Further, AVP has been implicated in human aggressive behavior, as levels of AVP in the cerebrospinal fluid of men and women were positively correlated with a life history of aggressive behaviors [46]. Taken together, these studies highlight the possibility that similar neural mechanisms may mediate social behaviors in humans and nonhuman mammals.

A relatively high degree of conservation between the behavioral and neurobiological aspects of prairie vole and human social behaviors suggests that the prairie vole model may be ideal for basic and translational research investigating the neurobiology of social behavior. Accordingly, research in prairie voles may not only allow us to learn more about the factors that underlie normal social behaviors, but may also enable us to explore the underlying causes of social deficits noted in several mental health disorders. A noteworthy example involves the use of the prairie vole model for the study of autism spectrum disorders [147, 157], in which AVP, OT, and DA have already been implicated [111, 115, 156, 236]. Additionally, the prairie vole has recently been established as an animal model for depression, specifically depression induced by social loss in adulthood [29, 103]. Finally, prairie voles have most recently been utilized to study the effects of drugs of abuse on pair bonding, and these studies have demonstrated that dysregulation of the mesolimbic DA system may be involved in the drug-induced impairment of social behavior [151, 238]. These and other studies [84] demonstrate the utility of this animal model for the investigation of neural mechanisms underlying normal and abnormal social behaviors, and their related processes.

Acknowledgments

We would like to thank C. Lieberwirth, K. Lei, M. M. Martin and A. S. Smith for their critical reading of the manuscript. We also thank A. S. Smith for his helpful discussions during the writing of this manuscript and for his photographic contributions. We gratefully acknowledge C. Badland and J. Chalcraft for their assistance with the figures. Funding for this work was provided by National Institutes of Health grants DAF31-25570 to KAY, MHF31-79600 to KLG, and MHR01-58616, MHR21-83128, DAR01-19627, and DAK02-23048, to ZW.

Footnotes

1While prairie voles that originated from Illinois display behaviors indicative of a monogamous life strategy in the field, and reliably display mating-induced pair bonding behaviors in laboratory conditions, it is important to note that prairie voles from Kansas [55, 186] and Tennessee [175, 235] show subtle differences in some aspects of their behavior and physiology [185] These differences support the theory that a variety of ecological conditions may influence animal behavior and mating strategies between populations within the same species [73]. As a result of this variation, prairie voles from Illinois are most commonly used in laboratory study of the neurobiology of pair bonding. Data from those studies are the focus of the current review.

Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

References

1. Abel T, Lattal KM. Molecular mechanisms of memory acquisition, consolidation and retrieval. Curr Opin Neurobiol. 2001;11:180–187. [PubMed]
2. Afonso VM, Sison M, Lovic V, Fleming AS. Medial prefrontal cortex lesions in the female rat affect sexual and maternal behavior and their sequential organization. Behav Neurosci. 2007;121:515–526. [PubMed]
3. Afonso VM, King S, Chatterjee D, Fleming AS. Hormones that increase maternal responsiveness affect accumbal dopaminergic responses to pup- and food-stimuli in the female rat. Horm Behav. 2009;56:11–23. [PubMed]
4. Ahern TH, Young LJ. The impact of early life family structure on adult social attachment, alloparental behavior, and the neuropeptide systems regulating affiliative behaviors in the monogamous prairie vole (Microtus ochrogaster) Front Behav Neurosci. 2009;3:17. [PMC free article] [PubMed]
5. Almond RE, Brown GR, Keverne EB. Suppression of prolactin does not reduce infant care by parentally experienced male common marmosets (Callithrix jacchus) Horm Behav. 2006;49:673–680. [PubMed]
6. Amico JA, Johnston JM, Vagnucci AH. Suckling-induced attenuation of plasma cortisol concentrations in postpartum lactating women. Endocr Res. 1994;20:79–87. [PubMed]
7. Aragona BJ, Liu Y, Curtis JT, Stephan FK, Wang Z. A critical role for nucleus accumbens dopamine in partner-preference formation in male prairie voles. J Neurosci. 2003;23:3483–3490. [PubMed]
8. Aragona BJ, Liu Y, Yu YJ, Curtis JT, Detwiler JM, Insel TR, Wang Z. Nucleus accumbens dopamine differentially mediates the formation and maintenance of monogamous pair bonds. Nat Neurosci. 2006;9:133–139. [PubMed]
9. Aragona BJ, Wang Z. Opposing regulation of pair bond formation by cAMP signaling within the nucleus accumbens shell. J Neurosci. 2007;27:13352–13356. [PubMed]
10. Aragona BJ, Wang Z. Dopamine regulation of social choice in a monogamous rodent species. Front Behav Neurosci. 2009;3:15. [PMC free article] [PubMed]
11. Argiolas A, Melis MR. Central control of penile erection: role of the paraventricular nucleus of the hypothalamus. Prog Neurobiol. 2005;76:1–21. [PubMed]
12. Aron A, Fisher H, Mashek DJ, Strong G, Li H, Brown LL. Reward, motivation, and emotion systems associated with early-stage intense romantic love. J Neurophysiol. 2005;94:327–337. [PubMed]
13. Bales KL, Kim AJ, Lewis-Reese AD, Sue Carter C. Both oxytocin and vasopressin may influence alloparental behavior in male prairie voles. Horm Behav. 2004;45:354–361. [PubMed]
14. Bales KL, Kramer KM, Lewis-Reese AD, Carter CS. Effects of stress on parental care are sexually dimorphic in prairie voles. Physiol Behav. 2006;87:424–429. [PubMed]
15. Bales KL, Mason WA, Catana C, Cherry SR, Mendoza SP. Neural correlates of pair-bonding in a monogamous primate. Brain Res. 2007;1184:245–253. [PMC free article] [PubMed]
16. Bales KL, van Westerhuyzen JA, Lewis-Reese AD, Grotte ND, Lanter JA, Carter CS. Oxytocin has dose-dependent developmental effects on pair-bonding and alloparental care in female prairie voles. Horm Behav. 2007;52:274–279. [PMC free article] [PubMed]
17. Bamshad M, Novak MA, deVries AC. Sex and species differences in the vasopressin innervation of sexually naive and parental prairie voles (Microtus ochrogaster) and meadow voles (Microtus pennsylvanicus) J Neuroendocrinol. 1993;5:247–255. [PubMed]
18. Bamshad M, Novak MA, De Vries GJ. Cohabitation alters vasopressin innervation and paternal behavior in prairie voles (Microtus ochrogaster) Physiol Behav. 1994;56:751–758. [PubMed]
19. Barberis C, Tribollet E. Vasopressin and oxytocin receptors in the central nervous system. Crit Rev Neurobiol. 1996;10:119–154. [PubMed]
20. Bartels A, Zeki S. The neural basis of romantic love. Neuroreport. 2000;11:3829–3834. [PubMed]
21. Bartels A, Zeki S. The neural correlates of maternal and romantic love. Neuroimage. 2004;21:1155–1166. [PubMed]
22. Becker JB, Rudick CN, Jenkins WJ. The role of dopamine in the nucleus accumbens and striatum during sexual behavior in the female rat. J Neurosci. 2001;21:3236–3241. [PubMed]
23. Berke JD, Hyman SE. Addiction, dopamine, and the molecular mechanisms of memory. Neuron. 2000;25:515–532. [PubMed]
24. Bester-Meredith JK, Young LJ, Marler CA. Species differences in paternal behavior and aggression in peromyscus and their associations with vasopressin immunoreactivity and receptors. Horm Behav. 1999;36:25–38. [PubMed]
25. Bester-Meredith JK, Marler CA. Vasopressin and aggression in cross-fostered California mice (Peromyscus californicus) and white-footed mice (Peromyscus leucopus) Horm Behav. 2001;40:51–64. [PubMed]
26. Bester-Meredith JK, Marler CA. Vasopressin and the transmission of paternal behavior across generations in mated, cross-fostered Peromyscus mice. Behav Neurosci. 2003;117:455–463. [PubMed]
27. Birney EC, Grant WE, Baird DD. Importance of vegetative cover to cycles of Microtus populations. Ecology. 1976;57:1043–1051.
28. Bogels S, Phares V. Fathers’ role in the etiology, prevention and treatment of child anxiety: a review and new model. Clin Psychol Rev. 2008;28:539–558. [PubMed]
29. Bosch OJ, Nair HP, Ahern TH, Neumann ID, Young LJ. The CRF system mediates increased passive stress-coping behavior following the loss of a bonded partner in a monogamous rodent. Neuropsychopharmacology. 2009;34:1406–1415. [PMC free article] [PubMed]
30. Brennan PA, Hancock D, Keverne EB. The expression of the immediate-early genes c-fos, egr-1 and c-jun in the accessory olfactory bulb during the formation of an olfactory memory in mice. Neuroscience. 1992;49:277–284. [PubMed]
31. Bridges RS. Neurobiology of the Parental Brain. Elsevier Inc; New York: 2008.
32. Buss DM. From vigilance to violence: tactics of mate retention in American undergraduates. Ethology and Sociobiology. 1988;9:291–317.
33. Canteras NS. The medial hypothalamic defensive system: hodological organization and functional implications. Pharmacol Biochem Behav. 2002;71:481–491. [PubMed]
34. Carr DB, Sesack SR. Terminals from the rat prefrontal cortex synapse on mesoaccumbens VTA neurons. Ann NY Acad Sci. 1999;877:676–678. [PubMed]
35. Carter CS, Witt DM, Auksi T, Casten L. Estrogen and the induction of lordosis in female and male prairie voles (Microtus ochrogaster) Horm Behav. 1987;21:65–73. [PubMed]
36. Carter CS, Witt DM, Schneider J, Harris ZL, Volkening D. Male stimuli are necessary for female sexual behavior and uterine growth in prairie voles (Microtus ochrogaster) Horm Behav. 1987;21:74–82. [PubMed]
37. Carter CS, Getz LL. Monogamy and the prairie vole. Sci Am. 1993;268:100–106. [PubMed]
38. Carter CS, DeVries AC, Getz LL. Physiological substrates of mammalian monogamy: the prairie vole model. Neurosci Biobehav Rev. 1995;19:303–314. [PubMed]
39. Carter CS, Altemus M. Integrative functions of lactational hormones in social behavior and stress management. In: Carter CS, Lederhendler II, Kirkpatrick B, editors. The Integrative Neurobiology of Affiliation. The MIT Press; Cambridge: 1999. pp. 361–371.
40. Cavanaugh BL, Lonstein JS. Androgenic and oestrogenic influences on tyrosine hydroxylase-immunoreactive cells of the prairie vole medial amygdala and bed nucleus of the stria terminalis. J Neuroendocrinol. 2010 [PMC free article] [PubMed]
41. Cherlin AJ, Furstenberg FF, Jr, Chase-Lansdale L, Kiernan KE, Robins PK, Morrison DR, Teitler JO. Longitudinal studies of effects of divorce on children in Great Britain and the United States. Science. 1991;252:1386–1389. [PubMed]
42. Chiodera P, Salvarani C, Bacchi-Modena A, Spallanzani R, Cigarini C, Alboni A, Gardini E, Coiro V. Relationship between plasma profiles of oxytocin and adrenocorticotropic hormone during suckling or breast stimulation in women. Horm Res. 1991;35:119–123. [PubMed]
43. Cho MM, DeVries AC, Williams JR, Carter CS. The effects of oxytocin and vasopressin on partner preferences in male and female prairie voles (Microtus ochrogaster) Behav Neurosci. 1999;113:1071–1079. [PubMed]
44. Ciliax BJ, Heilman C, Demchyshyn LL, Pristupa ZB, Ince E, Hersch SM, Niznik HB, Levey AI. The dopamine transporter: immunochemical characterization and localization in brain. J Neurosci. 1995;15:1714–1723. [PubMed]
45. Clutton-Brock TH. Mammalian mating systems. Proc R Soc Lond B Biol Sci. 1989;236:339–372. [PubMed]
46. Coccaro EF, Kavoussi RJ, Hauger RL, Cooper TB, Ferris CF. Cerebrospinal fluid vasopressin levels: correlates with aggression and serotonin function in personality-disordered subjects. Arch Gen Psychiatry. 1998;55:708–714. [PubMed]
47. Cohen-Parsons M, Carter CS. Males increase serum estrogen and estrogen receptor binding in brain of female voles. Physiol Behav. 1987;39:309–314. [PubMed]
48. Crenshaw BJ, De Vries GJ, Yahr P. Vasopressin innervation of sexually dimorphic structures of the gerbil forebrain under various hormonal conditions. J Comp Neurol. 1992;322:589–598. [PubMed]
49. Curtis JT, Liu Y, Wang Z. Lesions of the vomeronasal organ disrupt mating-induced pair bonding in female prairie voles (Microtus ochrogaster) Brain Res. 2001;901:167–174. [PubMed]
50. Curtis JT, Berkley KJ, Wang ZX. Neuronal activation in the caudal brainstem associated with mating by voles. Neurosci Lett. 2003;341:115–118. [PubMed]
51. Curtis JT, Wang Z. Forebrain c-fos expression under conditions conducive to pair bonding in female prairie voles (Microtus ochrogaster) Physiol Behav. 2003;80:95–101. [PubMed]
52. Curtis JT, Wang Z. Glucocorticoid receptor involvement in pair bonding in female prairie voles: the effects of acute blockade and interactions with central dopamine reward systems. Neuroscience. 2005;134:369–376. [PubMed]
53. Curtis JT, Wang Z. Ventral tegmental area involvement in pair bonding in male prairie voles. Physiol Behav. 2005;86:338–346. [PubMed]
54. Cushing BS, Carter CS. Peripheral pulses of oxytocin increase partner preferences in female, but not male, prairie voles. Horm Behav. 2000;37:49–56. [PubMed]
55. Cushing BS, Martin JO, Young LJ, Carter CS. The effects of peptides on partner preference formation are predicted by habitat in prairie voles. Horm Behav. 2001;39:48–58. [PubMed]
56. Cushing BS, Mogekwu N, Le WW, Hoffman GE, Carter CS. Cohabitation induced Fos immunoreactivity in the monogamous prairie vole. Brain Res. 2003;965:203–211. [PubMed]
57. Cushing BS, Okorie U, Young LJ. The effects of neonatal castration on the subsequent behavioural response to centrally administered arginine vasopressin and the expression of V1a receptors in adult male prairie voles. J Neuroendocrinol. 2003;15:1021–1026. [PubMed]
58. Cushing BS, Perry A, Musatov S, Ogawa S, Papademetriou E. Estrogen receptors in the medial amygdala inhibit the expression of male prosocial behavior. J Neurosci. 2008;28:10399–10403. [PMC free article] [PubMed]
59. de Jong TR, Chauke M, Harris BN, Saltzman W. From here to paternity: neural correlates of the onset of paternal behavior in California mice (Peromyscus californicus) Horm Behav. 2009;56:220–231. [PubMed]
60. De Vries GJ, Buijs RM. The origin of the vasopressinergic and oxytocinergic innervation of the rat brain with special reference to the lateral septum. Brain Res. 1983;273:307–317. [PubMed]
61. De Vries GJ, Buijs RM, Van Leeuwen FW. Sex differences in vasopressin and other neurotransmitter systems in the brain. Prog Brain Res. 1984;61:185–203. [PubMed]
62. De Vries GJ, Villalba C. Brain sexual dimorphism and sex differences in parental and other social behaviors. Ann NY Acad Sci. 1997;807:273–286. [PubMed]
63. DeVries AC, DeVries MB, Taymans S, Carter CS. Modulation of pair bonding in female prairie voles (Microtus ochrogaster) by corticosterone. Proc Natl Acad Sci USA. 1995;92:7744–7748. [PubMed]
64. DeVries AC, DeVries MB, Taymans SE, Carter CS. The effects of stress on social preferences are sexually dimorphic in prairie voles. Proc Natl Acad Sci USA. 1996;93:11980–11984. [PubMed]
65. DeVries AC, Cho MM, Cardillo S, Carter CS. Society for Neuroscience Abstract. 1997. Oxytocin can suppress the HPA axis in prairie voles.
66. DeVries AC, Taymans SE, Carter CS. Social modulation of corticosteroid responses in male prairie voles. Ann NY Acad Sci. 1997;807:494–497. [PubMed]
67. DeVries AC, Guptaa T, Cardillo S, Cho M, Carter CS. Corticotropin-releasing factor induces social preferences in male prairie voles. Psychoneuroendocrinology. 2002;27:705–714. [PubMed]
68. Dewsbury DA. Diversity and adaptation in rodent copulatory behavior. Science. 1975;190:947–954. [PubMed]
69. Dewsbury DA. The comparative psychology of monogamy. Nebraska Symposium on Motivation. 1987;35:1–50. [PubMed]
70. Ditzen B, Schaer M, Gabriel B, Bodenmann G, Ehlert U, Heinrichs M. Intranasal oxytocin increases positive communication and reduces cortisol levels during couple conflict. Biol Psychiatry. 2009;65:728–731. [PubMed]
71. Dubois D, Eitel S, Felner R. Effects of family environment and parent-child relationships on school adjustment during the transition to early adolescence. J Marr Fam. 1994;56:405–414.
72. Dudley CA, Rajendren G, Moss RL. Induction of FOS immunoreactivity in central accessory olfactory structures of the female rat following exposure to conspecific males. Mol Cell Neurosci. 1992;3:360–369. [PubMed]
73. Emlen ST, Oring LW. Ecology, sexual selection, and the evolution of mating systems. Science. 1977;197:215–223. [PubMed]
74. Everitt BJ. Sexual motivation: a neural and behavioural analysis of the mechanisms underlying appetitive and copulatory responses of male rats. Neurosci Biobehav Rev. 1990;14:217–232. [PubMed]
75. Fabiano GA. Father participation in behavioral parent training for ADHD: review and recommendations for increasing inclusion and engagement. J Fam Psychol. 2007;21:683–693. [PubMed]
76. Fahrbach SE, Morrell JI, Pfaff DW. Oxytocin induction of short-latency maternal behavior in nulliparous, estrogen-primed female rats. Horm Behav. 1984;18:267–286. [PubMed]
77. Ferguson JN, Aldag JM, Insel TR, Young LJ. Oxytocin in the medial amygdala is essential for social recognition in the mouse. J Neurosci. 2001;21:8278–8285. [PubMed]
78. Ferguson JN, Young LJ, Insel TR. The neuroendocrine basis of social recognition. Front Neuroendocrinol. 2002;23:200–224. [PubMed]
79. Ferris CF, Albers HE, Wesolowski SM, Goldman BD, Luman SE. Vasopressin injected into the hypothalamus triggers a stereotypic behavior in golden hamsters. Science. 1984;224:521–523. [PubMed]
80. Fisher H. Lust, attraction, and attachment in mammalian reproduction. Human Nature. 1998;9:23–52.
81. Fisher H, Aron A, Brown LL. Romantic love: an fMRI study of a neural mechanism for mate choice. J Comp Neurol. 2005;493:58–62. [PubMed]
82. FitzGerald RW, Madison DM. Social organization of a free-ranging population of pine volesMicrotus pinetorum. Behav Ecol Sociobiol. 1983;13:183–187.
83. Florsheim P, Tolan P, Gorman-Smith D. Family relationships, parenting practices, the availability of male family members, and the behavior of inner-city boys in single-mother and two-parent families. Child Dev. 1998;69:1437–1447. [PubMed]
84. Fowler CD, Liu Y, Ouimet C, Wang Z. The effects of social environment on adult neurogenesis in the female prairie vole. J Neurobiol. 2002;51:115–128. [PubMed]
85. Fraley RC, Shaver PR. Adult romantic attachment: theoretical developments, emerging controversies, and unanswered questions. Rev Gen Psych. 2000;4:132–154.
86. Fraley RC, Brumbaugh CC, Marks MJ. The evolution and function of adult attachment: a comparative and phylogenetic analysis. J Pers Soc Psychol. 2005;89:731–746. [PubMed]
87. Fuchs A-R, Saito S. Pituitary oxytocin and vasopressin content of pregnant rats before, during, and after parturition. Endocrinology. 1971;88:574–578. [PubMed]
88. Furstenberg FF, Jr, Teitler JO. Reconsidering the effects of marital disruption. J Fam Iss. 1994;15:173–190.
89. Geary DC. Evolution and proximate expression of human paternal investment. Psychol Bull. 2000;126:55–77. [PubMed]
90. Gerfen CR. The neostriatal mosaic: multiple levels of compartmental organization. Trends Neurosci. 1992;15:133–139. [PubMed]
91. Getz LL. Social structure and aggressive behavior in a population of Microtus pennsylvanicus. J Mammal. 1972;53:310–317.
92. Getz LL. Speculation on social structure and population cycles of Microtine rodents. The Biologist. 1978;60:134–147.
93. Getz LL, Cater CS. Social organizations in Microtus ochrogaster populations. The Biologist. 1980;62:1–4.
94. Getz LL, Carter SC, Gavish L. The mating system of the prairie vole, Microtus ochrogaster: field and laboratory evidence for pair-bonding. Behav Ecol Sociobiol. 1981;8:189–194.
95. Getz LL, Hofmann JE. Social organizations in free-living prairie voles Microtus ochrogaster. Behav Ecol Sociobiol. 1986;18:275–282.
96. Getz LL, Mc Guire B. A comparison of living singly and in male-female pairs in the prairie voleMicrotus ochrogaster. Ethology. 1993;94:265–278.
97. Getz LL, Carter CS. Prairie-vole partnerships. American Scientist. 1996;84:56–62.
98. Gingrich B, Liu Y, Cascio C, Wang Z, Insel TR. Dopamine D2 receptors in the nucleus accumbens are important for social attachment in female prairie voles (Microtus ochrogaster) Behav Neurosci. 2000;114:173–183. [PubMed]
99. Gobrogge KL, Liu Y, Jia X, Wang Z. Anterior hypothalamic neural activation and neurochemical associations with aggression in pair-bonded male prairie voles. J Comp Neurol. 2007;502:1109–1122. [PubMed]
100. Gobrogge KL, Liu Y, Young LJ, Wang Z. Anterior hypothalamic vasopressin regulates pair-bonding and drug-induced aggression in a monogamous rodent. Proc Natl Acad Sci USA. 2009;106:19144–19149. [PubMed]
101. Gobrogge KL, Wang ZX. Society for Neuroscience Abstract Poster #3774. 2009. Neural circuitry underlying pair-bonding-induced aggression in monogamous male prairie voles.
102. Gray G, Dewsbury D. A quantitative description of copulatory behavior in prairie voles (Microtus ochrogaster)Brain. Behav Evol. 1973;8:437–452. [PubMed]
103. Grippo AJ, Cushing BS, Carter CS. Depression-like behavior and stressor-induced neuroendocrine activation in female prairie voles exposed to chronic social isolation. Psychosom Med. 2007;69:149–157. [PMC free article] [PubMed]
104. Gruder-Adams S, Getz LL. Comparison of the mating system and paternal behavior in Microtus ochrogaster and Mictrotus pennsylvanicus. J Mammology. 1985;66:165–167.
105. Gubernick DJ. Biparental care and male-female relations in mammals. In: Parmigiani S, vom Saal FS, editors. Infanticide and Parental Care. Harwood Academic Publishers; Chur, Switzerland: 1994. pp. 427–463.
106. Hall ER. The Mammals of North America. John Wiley; New York: 1981.
107. Hammock EA, Young LJ. Variation in the vasopressin V1a receptor promoter and expression: implications for inter- and intraspecific variation in social behaviour. Eur J Neurosci. 2002;16:399–402. [PubMed]
108. Hammock EA, Young LJ. Functional microsatellite polymorphism associated with divergent social structure in vole species. Mol Biol Evol. 2004;21:1057–1063. [PubMed]
109. Hansen S, Bergvall AH, Nyiredi S. Interaction with pups enhances dopamine release in the ventral striatum of maternal rats: a microdialysis study. Pharmacol Biochem Behav. 1993;45:673–676. [PubMed]
110. Hastings NB, Orchinik M, Aubourg MV, McEwen BS. Pharmacological characterization of central and peripheral type I and type II adrenal steroid receptors in the prairie vole, a glucocorticoid-resistant rodent. Endocrinology. 1999;140:4459–4469. [PubMed]
111. Heinrichs M, von Dawans B, Domes G. Oxytocin, vasopressin, and human social behavior. Front Neuroendocrinol. 2009;30:548–557. [PubMed]
112. Hernandez-Gonzalez M, Navarro-Meza M, Prieto-Beracoechea CA, Guevara MA. Electrical activity of prefrontal cortex and ventral tegmental area during rat maternal behavior. Behav Processes. 2005;70:132–143. [PubMed]
113. Hnatczuk OC, Lisciotto CA, DonCarlos LL, Carter CS, Morrell JI. Estrogen receptor immunoreactivity in specific brain areas of the prairie vole (Microtus ochrogaster) is altered by sexual receptivity and genetic sex. J Neuroendocrinol. 1994;6:89–100. [PubMed]
114. Hokfelt T, Martensson R, Bjorklund A, Kleinau S, Goldstein M, Bjorklund A, Hokfelt T. Handbook of Chemical Neuroanatomy. Elsevier; Amsterdam: 1984. Distributional maps of tyrosine-hydroxylase-immunoreactive neurons in the rat brain.
115. Hollander E, Bartz J, Chaplin W, Phillips A, Sumner J, Soorya L, Anagnostou E, Wasserman S. Oxytocin increases retention of social cognition in autism. Biol Psychiatry. 2007;61:498–503. [PubMed]
116. House JS, Landis KR, Umberson D. Social relationships and health. Science. 1988;241:540–545. [PubMed]
117. Hull EM, Rodriguez-Manzo G. Male sexual behavior, Horm. Brain and Behav. 2009;1:5–65.
118. Hume JM, Wynne-Edwards KE. Castration reduces male testosterone, estradiol, and territorial aggression, but not paternal behavior in biparental dwarf hamsters (Phodopus campbelli) Horm Behav. 2005;48:303–310. [PubMed]
119. Hyman SE. Addiction: a disease of learning and memory. Am J Psychiatry. 2005;162:1414–1422. [PubMed]
120. Ikemoto S, Panksepp J. The role of nucleus accumbens dopamine in motivated behavior: a unifying interpretation with special reference to reward-seeking. Brain Res Brain Res Rev. 1999;31:6–41. [PubMed]
121. Inamura K, Kashiwayanagi M, Kurihara K. Regionalization of Fos immunostaining in rat accessory olfactory bulb when the vomeronasal organ was exposed to urine. Eur J Neurosci. 1999;11:2254–2260. [PubMed]
122. Insel TR, Shapiro LE. Oxytocin receptor distribution reflects social organization in monogamous and polygamous voles. Proc Natl Acad Sci, USA. 1992;89:5981–5985. [PubMed]
123. Insel TR, Wang ZX, Ferris CF. Patterns of brain vasopressin receptor distribution associated with social organization in microtine rodents. J Neurosci. 1994;14:5381–5392. [PubMed]
124. Insel TR, Preston S, Winslow JT. Mating in the monogamous male: behavioral consequences. Physiol Behav. 1995;57:615–627. [PubMed]
125. Insel TR, Young LJ. The neurobiology of attachment. Nat Rev Neurosci. 2001;2:129–136. [PubMed]
126. Jannett FJ. Social dynamics of the montane vole, Microtus montanus, as a paradigm. The Biologist. 1980;62:3–19.
127. Jannett FJ. Nesting patterns of adult vole, Microtus montanus, in field population. J Mammal. 1982;63:495–498.
128. Jansson A, Goldstein M, Tinner B, Zoli M, Meador-Woodruff JH, Lew JY, Levey AI, Watson S, Agnati LF, Fuxe K. On the distribution patterns of D1, D2, tyrosine hydroxylase and dopamine transporter immunoreactivities in the ventral striatum of the rat. Neuroscience. 1999;89:473–489. [PubMed]
129. Kendrick KM, Keverne EB, Baldwin BA. Intracerebroventricular oxytocin stimulates maternal behaviour in the sheep. Neuroendocrinology. 1987;46:56–61. [PubMed]
130. Kevetter GA, Winans SS. Connections of the corticomedial amygdala in the golden hamster. I. Efferents of the “vomeronasal amygdala” J Comp Neurol. 1981;197:81–98. [PubMed]
131. Kiecolt-Glaser JK, Newton TL. Marriage and health: his and hers. Psychol Bull. 2001;127:472–503. [PubMed]
132. Kikusui T, Winslow JT, Mori Y. Social buffering: relief from stress and anxiety. Philos Trans R Soc Lond B Biol Sci. 2006;361:2215–2228. [PMC free article] [PubMed]
133. Kirkpatrick B, Carter CS, Newman SW, Insel TR. Axon-sparing lesions of the medial nucleus of the amygdala decrease affiliative behaviors in the prairie vole (Microtus ochrogaster): behavioral and anatomical specificity. Behav Neurosci. 1994;108:501–513. [PubMed]
134. Kirkpatrick B, Kim JW, Insel TR. Limbic system fos expression associated with paternal behavior. Brain Res. 1994;658:112–118. [PubMed]
135. Kirkpatrick B, Williams JR, Slotnick BM, Carter CS. Olfactory bulbectomy decreases social behavior in male prairie voles (M. ochrogaster) Physiol Behav. 1994;55:885–889. [PubMed]
136. Kleiman DG. Monogamy in mammals. Q Rev Biol. 1977;52:39–69. [PubMed]
137. Kosfeld M, Heinrichs M, Zak PJ, Fischbacher U, Fehr E. Oxytocin increases trust in humans. Nature. 2005;435:673–676. [PubMed]
138. Kramer KM, Perry AN, Golbin D, Cushing BS. Sex steroids are necessary in the second postnatal week for the expression of male alloparental behavior in prairie voles (Microtus ochragaster) Behav Neurosci. 2009;123:958–963. [PubMed]
139. Lansing SW, Lonstein JS. Tyrosine hydroxylase-synthesizing cells in the hypothalamus of prairie voles (Microtus ochrogaster): sex differences in the anteroventral periventricular preoptic area and effects of adult gonadectomy or neonatal gonadal hormones. J Neurobiol. 2006;66:197–204. [PubMed]
140. Lei K, Cushing BS, Musatov S, Ogawa S, Kramer KM. Estrogen receptor-alpha in the bed nucleus of the stria terminalis regulates social affiliation in male prairie voles (Microtus ochrogaster) PLoS One. 2010;5:e8931. [PMC free article] [PubMed]
141. Lemon N, Manahan-Vaughan D. Dopamine D1/D5 receptors gate the acquisition of novel information through hippocampal long-term potentiation and long-term depression. J Neurosci. 2006;26:7723–7729. [PubMed]
142. Leng G, Meddle SL, Douglas AJ. Oxytocin and the maternal brain. Curr Opin Pharmacol. 2008;8:731–734. [PubMed]
143. Lightman SL. The neuroendocrinology of stress: a never ending story. J Neuroendocrinol. 2008;20:880–884. [PubMed]
144. Lillard LA, Waite LJ. ‘Til death do us part: marital disruption and mortality. American Journal of Sociology. 1995;100:1131–1156.
145. Lim MM, Wang Z, Olazabal DE, Ren X, Terwilliger EF, Young LJ. Enhanced partner preference in a promiscuous species by manipulating the expression of a single gene. Nature. 2004;429:754–757. [PubMed]
146. Lim MM, Young LJ. Vasopressin-dependent neural circuits underlying pair bond formation in the monogamous prairie vole. Neuroscience. 2004;125:35–45. [PubMed]
147. Lim MM, Bielsky IF, Young LJ. Neuropeptides and the social brain: potential rodent models of autism. Int J Dev Neurosci. 2005;23:235–243. [PubMed]
148. Lim MM, Liu Y, Ryabinin AE, Bai Y, Wang Z, Young LJ. CRF receptors in the nucleus accumbens modulate partner preference in prairie voles. Horm Behav. 2007;51:508–515. [PMC free article] [PubMed]
149. Liu Y, Curtis JT, Wang Z. Vasopressin in the lateral septum regulates pair bond formation in male prairie voles (Microtus ochrogaster) Behav Neurosci. 2001;115:910–919. [PubMed]
150. Liu Y, Wang ZX. Nucleus accumbens oxytocin and dopamine interact to regulate pair bond formation in female prairie voles. Neuroscience. 2003;121:537–544. [PubMed]
151. Liu Y, Aragona BJ, Young KA, Dietz DM, Kabbaj M, Mazei-Robison M, Nestler EJ, Wang Z. Nucleus accumbens dopamine mediates amphetamine-induced impairment of social bonding in a monogamous rodent species. Proc Natl Acad Sci USA. 2010;107:1217–1222. [PubMed]
152. Lonstein JS, De Vries GJ. Sex differences in the parental behaviour of adult virgin prairie voles: independence from gonadal hormones and vasopressin. J Neuroendocrinol. 1999;11:441–449. [PubMed]
153. Lonstein JS. Effects of dopamine receptor antagonism with haloperidol on nurturing behavior in the biparental prairie vole. Pharmacol Biochem Behav. 2002;74:11–19. [PubMed]
154. Madison DM. Movement indications of reproductive events among female meadow voles as revealed by radiotelemetry. J Mammol. 1978;59:835–843.
155. Madison DM. An integrated view of the social biology of Microtus pennsylvanicus. The Biologist. 1980;62:20–30.
156. McDougle CJ, Erickson CA, Stigler KA, Posey DJ. Neurochemistry in the pathophysiology of autism. J Clin Psychiatry 66 Suppl. 2005;10:9–18. [PubMed]
157. McGraw LA, Young LJ. The prairie vole: an emerging model organism for understanding the social brain. Trends Neurosci. 2010;33:103–109. [PMC free article] [PubMed]
158. McGuire B, Novak MA. A comparison of maternal behaviour in the meadow vole (Microtus pennsylvanicus), prairie vole (M. ochrogaster) and pine vole (M. pinetorum) Anim Behav. 1984;32:1132–1141.
159. McGuire B, Getz LL, Hofmann J, Pizzuto T, Frase B. Natal dispersal and philopatry in prairie voles (Microtus ochrogaster) in relation to population density, season, and natal social environment. Behav Ecol Sociobiol. 1993;32:293–302.
160. Mendoza SP, Mason WA. Parental division of labour and differentiation of attachments in a monogamous primate (Callicebus moloch) Anim Behav. 1986;34:1336–1347.
161. Mendoza SP, Mason WA. Parenting within a monogamous society. In: Else JG, Lee PC, editors. Primate Ontogeny, Cognition and Social Behavior. Cambridge University Press; Cambridge: 1986. pp. 255–266.
162. Miczek KA, Winslow JT. Psychopharmacologic research on aggressive behavior. In: Greenshaw AJ, Dourish CT, editors. Experimental Psychopharmacology. Humana Press; Clifton, NJ: 1987. pp. 27–113.
163. Missale C, Nash SR, Robinson SW, Jaber M, Caron MG. Dopamine receptors: from structure to function. Physiol Rev. 1998;78:189–225. [PubMed]
164. Mitchell JB, Gratton A. Mesolimbic dopamine release elicited by activation of the accessory olfactory system: a high speed chronoamperometric study. Neurosci Lett. 1992;140:81–84. [PubMed]
165. Neumann ID. Brain oxytocin: a key regulator of emotional and social behaviours in both females and males. J Neuroendocrinol. 2008;20:858–865. [PubMed]
166. Neve KA, Seamans JK, Trantham-Davidson H. Dopamine receptor signaling. J Recept Signal Transduct Res. 2004;24:165–205. [PubMed]
167. Nirenberg MJ, Chan J, Pohorille A, Vaughan RA, Uhl GR, Kuhar MJ, Pickel VM. The dopamine transporter: comparative ultrastructure of dopaminergic axons in limbic and motor compartments of the nucleus accumbens. J Neurosci. 1997;17:6899–6907. [PubMed]
168. Northcutt KV, Wang Z, Lonstein JS. Sex and species differences in tyrosine hydroxylase-synthesizing cells of the rodent olfactory extended amygdala. J Comp Neurol. 2007;500:103–115. [PubMed]
169. Northcutt KV, Lonstein JS. Social contact elicits immediate-early gene expression in dopaminergic cells of the male prairie vole extended olfactory amygdala. Neuroscience. 2009;163:9–22. [PMC free article] [PubMed]
170. Numan M, Insel TR. The Neurobiology of Parental Behavior. Springer-Verlag; New York, USA: 2003.
171. Numan M, Stolzenberg DS. Medial preoptic area interactions with dopamine neural systems in the control of the onset and maintenance of maternal behavior in rats. Front Neuroendocrinol. 2009;30:46–64. [PubMed]
172. Olazabal DE, Young LJ. Species and individual differences in juvenile female alloparental care are associated with oxytocin receptor density in the striatum and the lateral septum. Horm Behav. 2006;49:681–687. [PubMed]
173. Olazabal DE, Young LJ. Oxytocin receptors in the nucleus accumbens facilitate “spontaneous” maternal behavior in adult female prairie voles. Neuroscience. 2006;141:559–568. [PubMed]
174. Oliveras D, Novak MA. A comparison of paternal behavior in the meadow vole, Microtus pennsylvanicus, the pine vole, Microtus pinetorum, and prairie vole, Microtus ochrogaster. Animal Behavior. 1986;34:519–526.
175. Ophir AG, Phelps SM, Sorin AB, Wolff JO. Morphological, genetic, and behavioral comparisons of two prairie vole populations in the field and laboratory. J Mammal. 2007;88:989–999.
176. Pedersen CA, Prange AJJ. Induction of maternal behavior in virgin rats after intracerebroentricular administraion of oxytocin. Proc Natl Acad Sci USA. 1979;76:6661–6665. [PubMed]
177. Pedersen CA, Ascher JA, Monroe YL, Prange AJ., Jr Oxytocin induces maternal behavior in virgin female rats. Science. 1982;216:648–650. [PubMed]
178. Pedersen CA, Vadlamudi SV, Boccia ML, Amico JA. Maternal behavior deficits in nulliparous oxytocin knockout mice. Genes Brain Behav. 2006;5:274–281. [PubMed]
179. Pfaus JG, Mendelson SD, Phillips AG. A correlational and factor analysis of anticipatory and consummatory measures of sexual behavior in the male rat. Psychoneuroendocrinology. 1990;15:329–340. [PubMed]
180. Pfaus JG, Damsma G, Wenkstern D, Fibiger HC. Sexual activity increases dopamine transmission in the nucleus accumbens and striatum of female rats. Brain Res. 1995;693:21–30. [PubMed]
181. Phares V, Compas BE. The role of fathers in child and adolescent psychopathology: make room for daddy. Psychol Bull. 1992;111:387–412. [PubMed]
182. Piovanotti MR, Vieira ML. Presence of the father and parental experience have differentiated effects on pup development in Mongolian gerbils (Meriones unguiculatus) Behav Processes. 2004;66:107–117. [PubMed]
183. Pitkow LJ, Sharer CA, Ren X, Insel TR, Terwilliger EF, Young LJ. Facilitation of affiliation and pair-bond formation by vasopressin receptor gene transfer into the ventral forebrain of a monogamous vole. J Neurosci. 2001;21:7392–7396. [PubMed]
184. Roberts RL, Zullo A, Gustafson EA, Carter CS. Perinatal steroid treatments alter alloparental and affiliative behavior in prairie voles. Horm Behav. 1996;30:576–582. [PubMed]
185. Roberts RL, Carter CS. Intraspecific variation and the presence of a father can influence the expression of monogamous and communal traits in prairie voles. Ann NY Acad Sci. 1997;807:559–562. [PubMed]
186. Roberts RL, Williams JR, Wang AK, Carter CS. Cooperative breeding and monogamy in prairie voles: influence of the sire and geographical variation. Anim Behav. 1998;55:1131–1140. [PubMed]
187. Ross HE, Cole CD, Smith Y, Neumann ID, Landgraf R, Murphy AZ, Young LJ. Characterization of the oxytocin system regulating affiliative behavior in female prairie voles. Neuroscience. 2009;162:892–903. [PMC free article] [PubMed]
188. Ross HE, Freeman SM, Spiegel LL, Ren X, Terwilliger EF, Young LJ. Variation in oxytocin receptor density in the nucleus accumbens has differential effects on affiliative behaviors in monogamous and polygamous voles. J Neurosci. 2009;29:1312–1318. [PMC free article] [PubMed]
189. Rowley MH, Christian JJ. Intraspecific aggression of Peromyscus leucopus. Behav Biol. 1976;17:249–253. [PubMed]
190. Samuel PA, Hostetler CM, Bales KL. Urocortin II increases spontaneous parental behavior in prairie voles (Microtus ochrogaster) Behav Brain Res. 2008;186:284–288. [PMC free article] [PubMed]
191. Sarkadi A, Kristiansson R, Oberklaid F, Bremberg S. Fathers’ involvement and children’s developmental outcomes: a systematic review of longitudinal studies. Acta Paediatr. 2008;97:153–158. [PubMed]
192. Schellinck HM, Smyth C, Brown R, Wilkinson M. Odor-induced sexual maturation and expression of c-fos in the olfactory system of juvenile female mice. Brain Res Dev Brain Res. 1993;74:138–141. [PubMed]
193. Sesack SR, Carr DB, Omelchenko N, Pinto A. Anatomical substrates for glutamate-dopamine interactions: evidence for specificity of connections and extrasynaptic actions. Ann NY Acad Sci. 2003;1003:36–52. [PubMed]
194. Shapiro LE, Dewsbury DA. Differences in affiliative behavior, pair bonding, and vaginal cytology in two species of vole (Microtus ochrogaster and M. montanus) J Comp Psychol. 1990;104:268–274. [PubMed]
195. Silva MR, Bernardi MM, Felicio LF. Effects of dopamine receptor antagonists on ongoing maternal behavior in rats. Pharmacol Biochem Behav. 2001;68:461–468. [PubMed]
196. Smeltzer MD, Curtis JT, Aragona BJ, Wang Z. Dopamine, oxytocin, and vasopressin receptor binding in the medial prefrontal cortex of monogamous and promiscuous voles. Neurosci Lett. 2006;394:146–151. [PubMed]
197. Smith AS, Agmo A, Birnie AK, French JA. Manipulation of the oxytocin system alters social behavior and attraction in pair-bonding primatesCallithrix penicillata. Horm Behav. 2010;57:255–262. [PMC free article] [PubMed]
198. Solomon NG. Current indirect fitness benefits associated with philopatry in juvenile prairie voles. Behav Ecol Sociobiol. 1991;29:277–282.
199. Solomon NG, French JA. The study of mammalian cooperative breeding. In: Solomon NG, French JA, editors. Cooperative Breeding in Mammals. Cambridge University Press; New York: 1997. pp. 1–10.
200. Stein JA, Milburn NG, Zane JI, Rotheram-Borus MJ. Paternal and maternal influences on problem behaviors among homeless and runaway youth. Am J Orthopsychiatry. 2009;79:39–50. [PMC free article] [PubMed]
201. Stribley JM, Carter CS. Developmental exposure to vasopressin increases aggression in adult prairie voles. Proc Natl Acad Sci USA. 1999;96:12601–12604. [PubMed]
202. Swanson LW. Cerebral hemisphere regulation of motivated behavior. Brain Res. 2000;886:113–164. [PubMed]
203. Taylor SA, Salo AL, Dewsbury DA. Estrus induction in four species of voles (Microtus) J Comp Psychol. 1992;106:366–373. [PubMed]
204. Taymans SE, DeVries AC, DeVries MB, Nelson RJ, Friedman TC, Castro M, Detera-Wadleigh S, Carter CS, Chrousos GP. The hypothalamic-pituitary-adrenal axis of prairie voles (Microtus ochrogaster): evidence for target tissue glucocorticoid resistance. Gen Comp Endocrinol. 1997;106:48–61. [PubMed]
205. Thomas J, Birney EC. Parental care and mating systems of the prairie vole, Microtus orchogaster. Behav Ecol Sociobiol. 1979;5:171–186.
206. United Nations, Department of International Economic and Social Affairs. Socioeconomic differentials in child mortality in developing countries. United Nations; New York: 1985.
207. van Leeuwen FW, Caffe AR, De Vries GJ. Vasopressin cells in the bed nucleus of the stria terminalis of the rat: sex differences and the influence of androgens. Brain Res. 1985;325:391–394. [PubMed]
208. Van Schaik CP, Dunbar RIM. The evolution of monogamy in large primates: a new hypothesis and some critical tests. Behavior. 1990;115:30–62.
209. Villalba C, Boyle PA, Caliguri EJ, De Vries GJ. Effects of the selective serotonin reuptake inhibitor fluoxetine on social behaviors in male and female prairie voles (Microtus ochrogaster) Horm Behav. 1997;32:184–191. [PubMed]
210. Voorn P, Jorritsma-Byham B, Van Dijk C, Buijs RM. The dopaminergic innervation of the ventral striatum in the rat: a light- and electron-microscopical study with antibodies against dopamine. J Comp Neurol. 1986;251:84–99. [PubMed]
211. Wakerley JB, Clarke G, Summerlee AJS. Milk ejection and its control. In: Knobil E, Neill JD, editors. The Physiology of Reproduction. Raven Press; New York: 1994. pp. 1131–1177.
212. Waltz M, Badura B, Pfaff H, Schott T. Marriage and the psychological consequences of a heart attack: a longitudinal study of adaptation to chronic illness after 3 years. Soc Sci Med. 1988;27:149–158. [PubMed]
213. Wang Z, Ferris CF, De Vries GJ. Role of septal vasopressin innervation in paternal behavior in prairie voles (Microtus ochrogaster) Proc Natl Acad Sci USA. 1994;91:400–404. [PubMed]
214. Wang Z, Smith W, Major DE, De Vries GJ. Sex and species differences in the effects of cohabitation on vasopressin messenger RNA expression in the bed nucleus of the stria terminalis in prairie voles (Microtus ochrogaster) and meadow voles (Microtus pennsylvanicus) Brain Res. 1994;650:212–218. [PubMed]
215. Wang Z, Liu Y, Young LJ, Insel TR. Developmental changes in forebrain vasopressin receptor binding in prairie voles (Microtus ochrogaster) and montane voles (Microtus montanus) Ann NY Acad Sci. 1997;807:510–513. [PubMed]
216. Wang Z, Young LJ, De Vries GJ, Insel TR. Voles and vasopressin: a review of molecular, cellular, and behavioral studies of pair bonding and paternal behaviors. Prog Brain Res. 1998;119:483–499. [PubMed]
217. Wang Z, Yu G, Cascio C, Liu Y, Gingrich B, Insel TR. Dopamine D2 receptor-mediated regulation of partner preferences in female prairie voles (Microtus ochrogaster): a mechanism for pair bonding? Behav Neurosci. 1999;113:602–611. [PubMed]
218. Wang ZX, Novak MA. Influence of the social environment on parental behavior and pup development of Meadow voles (Microtus pennsylvanicus) and prairie voles (Microtus ochrogaster) J Comp Psychol. 1992;106:163–171.
219. Wang ZX, De Vries GJ. Testosterone effects on paternal behavior and vasopressin immunoreactive projections in prairie voles (Microtus ochrogaster) Brain Res. 1993;631:156–160. [PubMed]
220. Wang ZX, Novak MA. Alloparental care and the influence of father presence on juvenile prairie voles, Microtus ochrogaster. Anim Behav. 1994;47:281–288.
221. Wang ZX. Species differences in the vasopressin-immunoreactive pathways in the bed nucleus of the stria terminalis and medial amygdaloid nucleus in prairie voles (Microtus ochrogaster) and meadow voles (Microtus pennsylvanicus) Behav Neurosci. 1995;109:305–311. [PubMed]
222. Wang ZX, Insel TR. Parental behavior in voles. Adv Study Behav. 1996;25:361–384.
223. Wang ZX, Zhou L, Hulihan TJ, Insel TR. Immunoreactivity of central vasopressin and oxytocin pathways in microtine rodents: a quantitative comparative study. J Comp Neurol. 1996;366:726–737. [PubMed]
224. Wang ZX, Hulihan TJ, Insel TR. Sexual and social experience is associated with different patterns of behavior and neural activation in male prairie voles. Brain Res. 1997;767:321–332. [PubMed]
225. Wang ZX, Young LJ, Liu Y, Insel TR. Species differences in vasopressin receptor binding are evident early in development: comparative anatomic studies in prairie and montane voles. J Comp Neurol. 1997;378:535–546. [PubMed]
226. Wang ZX, Liu Y, Young LJ, Insel TR. Hypothalamic vasopressin gene expression increases in both males and females postpartum in a biparental rodent. J Neuroendocrinol. 2000;12:111–120. [PubMed]
227. Wideman CH, Murphy HM. Vasopressin, maternal behavior, and pup well-being. Cur Psych: Res Rev. 1990;9:285–295.
228. Williams JR, Carter CS, Insel T. Partner preference development in female prairie voles is facilitated by mating or the central infusion of oxytocin. Ann NY Acad Sci. 1992;652:487–489. [PubMed]
229. Williams JR, Catania KC, Carter CS. Development of partner preferences in female prairie voles (Microtus ochrogaster): the role of social and sexual experience. Horm Behav. 1992;26:339–349. [PubMed]
230. Wilson SC. Parent-Young contact in prairie and meadow voles. J Mammal. 1982;63:301–305.
231. Winslow JT, Hastings N, Carter CS, Harbaugh CR, Insel TR. A role for central vasopressin in pair bonding in monogamous prairie voles. Nature. 1993;365:545–548. [PubMed]
232. Wise RA. Dopamine, learning and motivation. Nat Rev Neurosci. 2004;5:483–494. [PubMed]
233. Witt DM, Carter CS, Lnsel TR. Oxytocin receptor binding in female prairie voles: endogenous and exogenous oestradiol stimulation. J Neuroendocrinol. 1991;3:155–161. [PubMed]
234. Wolf ME, Mangiavacchi S, Sun X. Mechanisms by which dopamine receptors may influence synaptic plasticity. Ann NY Acad Sci. 2003;1003:241–249. [PubMed]
235. Wolff JO, Dunlap AS. Multi-male mating, probability of conception, and litter size in the prairie vole (Microtus ochrogaster) Behav Processes. 2002;58:105–110. [PubMed]
236. Yirmiya N, Rosenberg C, Levi S, Salomon S, Shulman C, Nemanov L, Dina C, Ebstein RP. Association between the arginine vasopressin 1a receptor (AVPR1a) gene and autism in a family-based study: mediation by socialization skills. Mol Psychiatry. 2006;11:488–494. [PubMed]
237. Young KA, Liu Y, Wang Z. The neurobiology of social attachment: A comparative approach to behavioral, neuroanatomical, and neurochemical studies. Comp Biochem Physiol C Toxicol Pharmacol. 2008;148:401–410. [PMC free article] [PubMed]
238. Young KA, Gobrogge KL, Wang ZX. The role of mesocorticolimbic dopamine in regulating interactions between drugs of abuse and social behavior. Neurosci Biobehav Rev. 2010 doi: 10.1016/j.neubiorev.2010.06.004. [PMC free article] [PubMed] [Cross Ref]
239. Young LJ, Huot B, Nilsen R, Wang Z, Insel TR. Species differences in central oxytocin receptor gene expression: comparative analysis of promoter sequences. J Neuroendocrinol. 1996;8:777–783. [PubMed]
240. Young LJ, Waymire KG, Nilsen R, Macgregor GR, Wang Z, Insel TR. The 5′ flanking region of the monogamous prairie vole oxytocin receptor gene directs tissue-specific expression in transgenic mice. Ann NY Acad Sci. 1997;807:514–517. [PubMed]
241. Young LJ, Winslow JT, Nilsen R, Insel TR. Species differences in V1a receptor gene expression in monogamous and nonmonogamous voles: behavioral consequences. Behav Neurosci. 1997;111:599–605. [PubMed]
242. Young LJ, Frank A. Beach Award. Oxytocin and vasopressin receptors and species-typical social behaviors. Horm Behav. 1999;36:212–221. [PubMed]
243. Young LJ, Nilsen R, Waymire KG, MacGregor GR, Insel TR. Increased affiliative response to vasopressin in mice expressing the V1a receptor from a monogamous vole. Nature. 1999;400:766–768. [PubMed]
244. Young LJ, Lim MM, Gingrich B, Insel TR. Cellular mechanisms of social attachment. Horm Behav. 2001;40:133–138. [PubMed]
245. Young LJ, Wang Z. The neurobiology of pair bonding. Nat Neurosci. 2004;7:1048–1054. [PubMed]
246. Ziegler TE, Jacoris S, Snowdon CT. Sexual communication between breeding male and female cotton-top tamarins (Saguinus oedipus), and its relationship to infant care. Am J Primatol. 2004;64:57–69. [PMC free article] [PubMed]