Lipid–arrays and live-cell imaging identify new series of sphingolipid effectors. (A) The effect of the perturbation of sphingolipid metabolism on GFP-fusion localization was monitored in yeast cells treated with myriocin, myriocin and dihydrosphingosine (DHS) or untreated (Blank). (B) The proteins-binding sphingolipids in vitro are grouped according to their sphingolipid-binding specificities, and ordered by function. The symbol # points to proteins with a PH domain. Interactions are color coded as in Figure 2, but excluding live-cell imaging. The green boxes label the proteins selected for further analysis by live-cell imaging. Those sensitive to 2 h myriocin treatments are represented with filled green boxes. For each sphingolipid-binding group, the histogram next to the matrix summarizes the overall sensitivities to myriocin. For comparison, myriocin sensitivity of 32 controls is also shown in magenta. Non-physiological lipids in S. cerevisiae are in gray and italic. DHS-1P, dihydrosphingosine-1-phosphate; PHS-1P, phytosphingosine-1-phosphate; Sph-1P, sphingosine-1-phosphate; 3-ketoDHS, 3-ketodihydrosphingosine; DHS, dihydrosphingosine; PHS, phytosphingosine; Sph, sphingosine; DHCer, dihydroceramide; PHCer C8, phytoceramide C8; PHCer C18, phytoceramide C18.
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