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Mol Syst Biol. 2010; 6: 430.
Published online 2010 November 30. doi:  10.1038/msb.2010.87
PMCID: PMC3010107

A systematic screen for protein–lipid interactions in Saccharomyces cerevisiae

Abstract

Protein–metabolite networks are central to biological systems, but are incompletely understood. Here, we report a screen to catalog protein–lipid interactions in yeast. We used arrays of 56 metabolites to measure lipid-binding fingerprints of 172 proteins, including 91 with predicted lipid-binding domains. We identified 530 protein–lipid associations, the majority of which are novel. To show the data set's biological value, we studied further several novel interactions with sphingolipids, a class of conserved bioactive lipids with an elusive mode of action. Integration of live-cell imaging suggests new cellular targets for these molecules, including several with pleckstrin homology (PH) domains. Validated interactions with Slm1, a regulator of actin polarization, show that PH domains can have unexpected lipid-binding specificities and can act as coincidence sensors for both phosphatidylinositol phosphates and phosphorylated sphingolipids.


Articles from Molecular Systems Biology are provided here courtesy of The European Molecular Biology Organization