Dietary insulin load, dietary insulin index, and colorectal cancer

doi:10.1158/1055-9965.EPI-10-0833

Cancer Epidemiol Biomarkers Prev. Author manuscript; available in PMC 2011 December 1.

Published in final edited form as:

Cancer Epidemiol Biomarkers Prev. 2010 December; 19(12): 3020–3026.

Published online 2010 October 5. doi: 10.1158/1055-9965.EPI-10-0833

PMCID: PMC3005557

NIHMSID: NIHMS242699

Dietary insulin load, dietary insulin index, and colorectal cancer

Ying Bao,¹ Katharina Nimptsch,² Jeffrey A. Meyerhardt,⁴ Andrew T. Chan,⁵ Kimmie Ng,⁴ Dominique S. Michaud,^3,⁶ Jennie C. Brand-Miller,⁷ Walter C. Willett,^2,³ Edward Giovannucci,^1,^2,³ and Charles S. Fuchs^1,⁴

¹Channing Laboratory, Department of Medicine, Brigham and Women’s Hospital and Harvard Medical School, Boston, MA

²Departments of Nutrition, Harvard School of Public Health, Boston, MA

³Departments of Epidemiology, Harvard School of Public Health, Boston, MA

⁴Department of Adult Oncology, Dana-Farber Cancer Institute, Boston, MA

⁵Massachusetts General Hospital and Harvard Medical School, Boston

⁶Department of Epidemiology and Public Health, Imperial College London, UK

⁷Institute of Obesity, Nutrition, and Exercise, University of Sydney, Sydney, Australia

Requests for reprints and correspondence to: Ying Bao, MD, ScD, Channing Laboratory, Department of Medicine, Brigham and Women’s Hospital and Harvard Medical School, 181 Longwood Avenue, Boston, MA 02115. Phone: (617) 525-2099; Fax: (617) 525-2008. Email: ying.bao/at/channing.harvard.edu

The publisher's final edited version of this article is available free at Cancer Epidemiol Biomarkers Prev

Abstract

Background

Circulating insulin levels have been positively associated with risk of colorectal cancer; however, it remains unclear whether a diet inducing an elevated insulin response influences colorectal cancer risk. Based on a novel insulin index for individual foods, we estimated insulin demand for overall diets and assessed its association with colorectal cancer in the Nurses’ Health Study and Health Professionals Follow-up Study.

Methods

We followed 86,740 women and 46,146 men who were free of cancer and diabetes at baseline and identified a total of 2,481 colorectal cancer cases during up to 26 years of follow-up. Dietary insulin load was calculated as a function of food insulin index and the energy content of individual foods reported on food frequency questionnaires. Average dietary insulin index was calculated by dividing the dietary insulin load by the total energy intake.

Results

Dietary insulin load and dietary insulin index were not associated with risk of colorectal cancer. Comparing the highest with the lowest quintiles, the pooled multivariate relative risks (RRs) of colorectal cancer were 0.91 (95% confidence interval [CI] = 0.79–1.05) for dietary insulin load and 0.93 (95% CI = 0.81–1.08) for dietary insulin index. Body mass index and physical activity did not modify the association of dietary insulin load or index with colorectal cancer.

Conclusion

A diet high in foods that increase postprandial insulin levels did not increase risk of colorectal cancer in this large prospective study.

Impact

This study is the first to investigate insulin index and load in relation to colorectal cancer.

INTRODUCTION

In many observational studies individuals with type 2 diabetes mellitus have had increased risk of colorectal cancer (1). As patients with type 2 diabetes usually have hyperinsulinemia in the early stage of their disease, and insulin has growth-promoting effects, increased insulin exposure has been hypothesized as a biological mechanism whereby diabetes may be related to colorectal carcinogenesis (2, 3). Also, chronic exogenous insulin therapy significantly increases risk of colorectal cancer among type 2 diabetes patients (4). Additionally, common risk factors for type 2 diabetes and colorectal cancer, such as physical inactivity, obesity, and visceral adiposity, have been related to insulin resistance and hyperinsulinemia (2, 3).

Higher circulating insulin or C-peptide (a marker of insulin resistance and long-term insulin secretion) has been associated with increased risk of colorectal cancer in many studies (5–12). However, whether a diet inducing an elevated insulin response influences colorectal cancer risk remains unclear. Previous studies have used glycemic load and glycemic index as indicators for insulin response, most of which found no association with colorectal cancer (13). Nonetheless, glycemic load and glycemic index only characterize the influence of carbohydrate on blood glucose, which may limit their capacity for accurate estimation of insulin response because in addition to carbohydrate, protein and fat can induce insulin secretion (14).

A novel insulin index may more directly address the insulin hypothesis because it quantifies postprandial insulin response for various food items, including those with low or no carbohydrate content (14). Based on this new concept, the insulin response to overall diets, represented by dietary insulin load and dietary insulin index, can be further calculated. A recent study evaluated the validity of dietary insulin load in predicting the actual insulin response to a composite meal among young healthy subjects (mean age 24; n = 10 or 11 for each meal) who consumed 13 different meals of varying macronutrient content (15). They found that dietary insulin load was strongly correlated with observed postprandial insulin responses (r = 0.78, P = 0.002), and it provided a more accurate prediction of insulin demand than carbohydrate content or glycemic load.

In the present study, we examined the associations between these two insulin scores and colorectal cancer risk. To our knowledge, this study is the first to use insulin index to investigate the effect of consuming a high insulinogenic diet on risk of colorectal cancer.

PARTICIPANTS AND METHODS

Study Population

The Nurses’ Health Study (NHS) enrolled 121,700 U.S. female nurses aged 30–55 years in 1976. The Health Professionals Follow-up Study (HPFS) enrolled 51,529 U.S. male health professionals aged 40–75 years in 1986. Participants completed a baseline questionnaire and biennial follow-up questionnaires; in the NHS diet was first assessed in 1980. The overall follow-up rate was over 90% in the NHS and 94% in the HPFS. At baseline for the dietary analyses, we excluded participants who had cancer, left an extensive number of items blank (>9 items on the 61-item food frequency questionnaire in 1980 for the women and >=70 items on the 131-item food frequency questionnaire in 1986 for the men), or reported implausible energy intake (<500 or >3,500 kilocalories per day for women and <800 or >4,200 kilocalories per day for men). Because diabetic patients usually change their diet, we also excluded individuals who had diabetes before baseline. This left a cohort of 132,886 participants eligible (86,740 women and 46,146 men). This study was approved by the Human Subjects Committee at Brigham and Women’s Hospital and the Harvard School of Public Health.

Assessment of Dietary and Nondietary Factors

Dietary information was collected at baseline and every 2 to 4 years thereafter. Insulin index values for individual food were obtained from published estimates (31 foods) (14, 16) or provided by Dr. Jennie Brand-Miller of the University of Sydney, Australia (73 foods). U.S. food samples were shipped to the laboratory in Sydney for testing. The testing procedure has been described in detail previously (14): each person consumed a variety of test foods on separate days, with insulin measured every 15 minutes for 2 hours after consumption. The food insulin index value was calculated by dividing the area under the insulin response curve for 1000 kilojoules of a test food by the area under the insulin response curve for 1000 kilojoules of the reference food (glucose). The insulin index value for each test food represented the mean responses of 11–13 subjects. Based on these new analytical data and the previously published estimates, we built an insulin index database for a large number of foods listed on the food frequency questionnaires.

Using the food insulin index, we calculated the average dietary insulin load for each participant by multiplying the insulin index value of each food by its energy content, and summing values for all food items reported [Σ(food insulin index × kilocalories per serving × servings per day)]. Each unit of dietary insulin load represents the equivalent insulin response generated by 1 kilocalorie of glucose.

The dietary insulin index for the overall diet, which is the weighted mean of the insulin index values for each of the component foods, was calculated by dividing the dietary insulin load by the total energy intake [Σ (kilocalories per serving × servings per day)].

Validation studies have shown that the food-frequency questionnaire is a reasonably accurate measure of a person’s food intake (17, 18). For food items that have high insulin index values, the correlation coefficients between the food frequency questionnaire and 1-week diet records were: 0.46 (NHS) and 0.66 (HPFS) for meat, 0.79 and 0.86 for cold breakfast cereal, 0.81 and 0.88 for skimmed milk, 0.77 and 0.37 for dark bread, and 0.71 and 0.45 for white bread.

Information on smoking, body mass index (BMI), physical activity, family history of colorectal cancer, diabetes (incident cases during follow-up), ulcerative colitis, polyps, lower endoscopy, aspirin use, and multivitamin use were updated every 2 to 4 years.

Case Ascertainment

We obtained self-reported information on the occurrence of colorectal cancer on each follow-up questionnaire and asked participants for permission to access medical records to confirm diagnosis. The National Death Index was also used to identify fatalities. A total of 2,481 (1,420 in the NHS and 1,061 in the HPFS) colorectal cancer cases were indentified. Among them, 1,761 (1,067 in the NHS and 694 in the HPFS) were colon cancer and 545 (323 in the NHS and 222 in the HPFS) were rectal cancer, the rest were not clearly classified for sub-site.

Statistical Analysis

The follow-up started from1980 in the NHS and 1986 in the HPFS, and ended with colorectal cancer diagnosis, death, or on June 30, 2006 in the NHS and January 31, 2006 in the HPFS. Dietary insulin load and dietary insulin index were energy-adjusted by the residual method (19). We first analyzed dietary insulin scores derived from baseline questionnaires and then did three alternative analyses: using the 1984 dietary questionnaire as baseline for the NHS (because the 1984 questionnaire had more food items), updating the scores every 4 years (simple update), and updating the scores cumulatively (cumulative update). In multivariate analyses, we adjusted for BMI, physical activity, family history of colorectal cancer, lower endoscopy, diabetes (incident cases during follow-up), ulcerative colitis, history of polyps, aspirin use, multivitamin use, smoking, alcohol, and energy intake. Quintiles of main exposures and covariates were based on the cohort-specific intake distributions. Tests for trend were performed using continuous variables of dietary insulin load and dietary insulin index. Results from the two cohorts were pooled to compute a summary risk estimate using a random effects model (20).

To examine whether the associations of interest were modified by the preexisting insulin resistance, we stratified analyses by BMI (above or below 27.5 kg/m²) and physical activity (above or below median). BMI of 27.5 kg/m² was used as the cutoff point because colon cancer risk mainly increased among more severe overweight or obese participants in our two cohorts (21, 22). Because high fiber intake may reduce insulin demand (23), we also examined the joint effect of insulin load and fiber intake by cross classifying participants by both variables. Tests for interaction were performed by the Wald test using cross-product terms.

RESULTS

At baseline, men and women with higher insulin load were less likely to smoke and consumed less alcohol (Table 1). Although dietary insulin load and dietary insulin index were inversely associated with colorectal cancer risk in age-adjusted models, multivariate analyses showed no association in men or women or men and women combined (Table 2). The pooled multivariate RRs of colorectal cancer for the highest versus the lowest quintile were 0.91 (95% CI = 0.79–1.05) for dietary insulin load and 0.93 (95% CI = 0.81–1.08) for dietary insulin index, and the pooled RRs did not differ greatly across quintiles. Separate analyses of colon and rectal cancer revealed no material differences in the associations with dietary insulin load or dietary insulin index (Table 2). A further division of colon cancer into proximal and distal colon cancer also did not alter the findings (data not shown).

Table 1

Baseline characteristics of participants by quintiles of energy-adjusted dietary insulin load^*

Table 2

Dietary insulin load, dietary insulin index, and risk of colorectal cancer

Dietary insulin load and dietary insulin index were not associated with colorectal cancer risk for individuals who were overweight, less active or both (Table 3). None of the P-values for the interactions was statistically significant (data not shown). Similarly, the association of insulin load with colorectal cancer risk did not vary by fiber intake: the pooled multivariate RRs for the combination of a high insulin load and a low fiber intake compared with the opposite extreme was 1.01 (95% CI=0.83–1.22) for total fiber and 1.03 (95% CI=0.72–1.46) for cereal fiber.

Table 3

Dietary insulin load, dietary insulin index, and risk of colorectal cancer, stratified by BMI and physical activity^*

We observed no associations when we used the 1984 dietary questionnaire as baseline for the NHS, or when we used simple or cumulative updating of the dietary insulin scores, or when dietary insulin load and index were not energy-adjusted by the residual method, or after excluding the first 2 years of follow-up, restricting to those without ulcerative colitis, or further adjusting for dietary glycemic load, dietary glycemic index, and intakes of red meat, fruit and vegetable, fiber, folate, calcium and vitamin D (data not shown).

DISCUSSION

We found little evidence that a diet with high insulin load or insulin index is related to colorectal cancer risk. Imprecise measurement of dietary insulin load and index could bias the results towards the null; however, the food insulin index, on which dietary insulin load and index were based, was developed under highly standardized conditions (14): the insulin index value for each food represented the mean insulin responses of 11–13 subjects who consumed the test food on separate days. In a validation study, dietary insulin load has been shown to be an accurate measure of actual postprandial insulin responses (15). Furthermore, in the NHS and HPFS, insulin scores was correlated with plasma triglycerides levels (a marker of insulin production), confirming that the estimation of dietary insulin load and index is able to predict an expected biological response (Dr. Katharina Nimptsch, personal communication).

The lack of association in the present study is consistent with most previous studies that examined glycemic load and glycemic index in relation to colorectal cancer. A recent meta-analysis of studies up to 2008 showed that the pooled RRs of colorectal cancer were 1.06 (95% CI=0.95–1.17; n=8 cohort studies) for glycemic load and 1.04 (95% CI=0.92–1.12; n=7 cohort studies) for glycemic index (13). In contrast, high blood insulin levels have been associated with increased risk of colorectal cancer in a number of serologic studies (5–12). A recent meta-analysis which summarized epidemiological studies up to 2007 (24) showed that the pooled RR of colorectal cancer was 1.35 (95% CI=1.13–1.61; n=10 prospective studies and 1 case-control study) comparing the highest versus lowest category of insulin or C-peptide.

One explanation for the disparate findings with serum insulin and insulinogenic diets is that long-term insulin levels may not be greatly influenced by the consumption of insulinogenic foods, because food intake increases postprandial insulin demand and therefore affects insulin levels only temporarily (2, 3). As insulin resistance greatly upregulates the long-term secretory tone and causes a compensatory increase in both basal insulin secretion and postload insulin responses, it is possible that insulin resistance, instead of insulinogenic food intake, is the primary contributor to the sustained hyperinsulinemia that is relevant to cancer development. In the prospective Northern Sweden Health and Disease Cohort, fasting insulin (which mainly reflects the degree of insulin resistance) was positively associated with colorectal cancer and no association was observed for a mix of fasting and nonfasting samples (which reflects both insulin resistance and the influence of insulinogenic foods) (7); in a subcohort of that study, C-peptide levels were positively associated with colorectal cancer risk among fasting women but not among nonfasting women (25). Several other studies found similar increased risk of colorectal cancer for both fasting C-peptide and a mix of fasting and nonfasting C-peptide (8, 12, 26); observed positive association with postprandial hyperinsulinemia may principally be due to underlying insulin resistance as well. These findings and our results suggest that high intake of insulinogenic foods alone might not be enough to induce sustained hyperinsulinemia and therefore less likely to influence colorectal cancer risk.

The insulin scores have limitations. They were developed to assess total quantity of insulinogenic food intake, but were not designed to measure meal frequency and food combinations which might also affect insulin response. Another concern is that the food insulin index values were derived from lean university students (14) whose absolute insulin response is likely to be different from that of the older and heavier subjects; however, the method is valid if the increase in insulin levels induced by a food, i.e., the relative insulin response, is comparable between the two groups. Actually, in the biomarker validation study (Dr. Katharina Nimptsch, personal communication), we observed that the positive association between the insulin index and triglycerides was much stronger among overweight individuals, indicating that the general method used to develop the insulin index works among heavier subjects.

In summary, our data suggest that high intake of foods that increase postprandial insulin levels may not play a major part in colorectal cancer development. Further studies should focus on the role of insulin resistance to provide a more precise and thorough understanding of the insulin-colorectal cancer hypothesis.

Acknowledgments

Funding: This study was supported by grants from the National Cancer Institute, National Institutes of Health, Bethesda, MD (P01 CA87969, P01 CA55075, and P50 CA127003).

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