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RalA expression in human prostate cancer is associated with cell migration and is necessary for bone metastasis. However, the downstream effectors of RalA that mediate these functions remain unclear. Here we examined cell migration after small interfering RNA-mediated depletion of Ral effectors Ral binding protein 1 (RalBP1/RLIP), exocyst complex component 2 (Sec5), and phospholipase D1 (PLD1) and found that RalBP1 and RalA depletion inhibited cell migration to a similar extent. Stable lentivirus short hairpin interfering RNA-mediated depletion of RalA and RalBP1 in PC3 human prostate cancer cells inhibited bone metastasis after intracardiac inoculation. Depletion of RalBP1 diminished orthotopic tumor growth of PC3 cells and inhibited spontaneous metastasis from this site. Interestingly, the expression of wild-type or RalA mutants deficient in RalBP1 binding was effective at rescuing the reduced metastatic capacity of RalA-depleted PC3 cells, suggesting that RalA depletion does not reduce this solely by diminished interaction with RalBP1. To determine whether the role of RalBP1 in metastasis is relevant beyond prostate cancer, we studied the requirement of RalBP1 expression in an experimental metastasis model of human bladder cancer, a tumor type with high RalBP1 expression. Depletion of RalBP1 in UMUC3 cells resulted in decreased lung colonization while having a minimal effect on subcutaneous tumor growth. Our studies are the first to suggest that the expression of RalBP1 is necessary for human cancer cell metastasis. Furthermore, we show that the requirement for RalA expression for manifestation of this phenotype is not entirely dependent on a RalA-RalBP1 interaction.
RalA and RalB GTPases are monomeric G proteins of the Ras superfamily with 82% amino acid identity . Ral proteins were initially implicated in Ras-mediated transformation of human cells  as well as in maintenance of the Ras-driven malignant phenotype . Recent evidence also indicates their essential role in cancer progression and metastasis. Both RalA and RalB have been shown to be involved in pancreatic cancer cell metastasis . In contrast, RalA but not RalB was necessary for prostate cancer PC3 cells to metastasize to bone after stable expression of RNA interference for Ral paralogs . We recently observed that RalA expression in bladder tumors is associated with higher stage . Experimentally, RalA and RalB depletion after transient small interfering RNA (siRNA)-mediated knockdown was associated with decreased cell growth and migration in both bladder and prostate cancer cells . However, the downstream signaling pathways that mediate Ral function in migration and metastasis are unclear.
On activation, Ral paralogs bind to several effectors including RalBP1 (RLIP), exocyst components (Sec5 and Exo84), filamin, phospholipase D1 (PLD1), and ZONAB, and this results in diverse Ral-associated functions . RalBP1 is the best-characterized Ral effector, and previous results suggested its association with Ral-mediated tumorigenesis . Furthermore, expression of RalBP1 is increased in human bladder cancer tissues compared with normal tissues, and its level was correlated with Ral expression . Depletion of RalBP1 by antisense or inhibition of its function by antibody decreased subcutaneous xenograft tumor growth in a number of cancer cell lines [8,9]. Similar to RalBP1, the exocyst complex component Sec5 has been shown to be involved in Ral-mediated transformation . PLD1 was also associated with cells transformation by oncogenes including Ras [10,11].
Although these Ral effectors have been implicated in transformation, their functional role in Ral-mediated cancer progression has not been clearly defined. Here, we begin by examining the function of Ral effectors on cell migration and find that RalBP1 depletion inhibited cell migration to a similar extent as RalA depletion did in human prostate cancer cells. Further examination of the consequences of stable depletion of RalBP1 in prostate and bladder cancer cells points to an important role for RalBP1 in cancer metastasis.
Human bladder cancer UMUC3 and prostate cancer PC-3 cell lines were obtained from American Type Culture Collection (Rockville, MD), and cultures were as described. For counting assays, cells were plated at a concentration of 1000 cells per well in 200 εl in 96-well tissue culture plates (Corning Incorporated, Corning, NY). Replicate plates were plated for each indicated time point. At each time point, 10 εl of Alamar blue was added to each well, and fluorescence was measured 4 hours later on a microplate reader (BioTek, Winooski, VT) with excitation at 520 nm and emission at 570 nm.
The NH2-terminally Flag-tagged RalA expression pFlag-CMV-4 vector was described previously . The RalA sequence 5′-CGATGAGTTTGTGGAGGACT-3′ was targeted by short hairpin interfering RNA (shRNA). Lentiviral shRNA expression vectors targeting RalA, RalB, and RalBP1 were purchased from Sigma (St Louis, MO). RalA-5 shRNA (5′-CCGGCGATGAGTTTGTGGAGGACTACTCGAGTAGTCCTCCACAAACTCATCGTTTTT-3′), RalB-4 shRNA (CCGGCCTTTACAGCAACTGCCGAATCTCGAGATTCGGCAGTTGCTGTAAAGGTTTTTG), RalBP1-1 shRNA (5′-CCGGCCAGAGAATTTGCTTACCAAACTCGAGTTTGGTAAGCAAATTCTCTGGTTTTTG-3′), and RalBP1-3 shRNA (5′-CCGGGCACAAGAGATAGCCAGTCTTCTCGAGAAGACTGGCTATCTCTTGTGCTTTTTG-3′) were encoded in a lentiviral expression vector, PLKO, which carries a puromycin selection marker. The same lentiviral vector containing a nontarget shRNA sequence that does not target any human gene was used as control. The SFGnesTGL vector expressing a thymidine kinase/green fluorescent protein/luciferase (TGL) fusion protein was described . All plasmid DNA used for transfection was prepared by the “EndoFree Plasmid Maxi kit“ from Qiagen (Valencia, CA).
siRNA duplexes were purchased from Dharmacon (Lafayette, CO). RalA targeting siRNA was described previously . RalBP1-targeting siRNA duplexes were a mixture of two individual duplex with the following targeting sequence: 5′-GAACGAAGAGCUGGAAAUAUU-3′ and 5′-GAAGGCAUCUACAGAGUAUUU-3′. siRNA duplexes targeting Sec5 and PLD1 were smart pools, and the siRNA duplex targeting luciferase (GL2), 5′-CGTACGCGGAATACTTCGA-3′, was used as control siRNA. Transfection of cells with siRNA duplexes (100 nM) was done using OligofectAMINE (Invitrogen, Carlsbad, CA).
Parental UMUC3 and PC3 cells were stably transfected with linearized SFGnesTGL vector, and luciferase-expressing cells were selected by fluorescence-activated cell sorting of green fluorescent protein. To generate stable RalA and RalBP1 knockdown cells, RalA and RalBP1 shRNA constructs were cotransfected with lentiviral package plasmids CMVΔR8.2 and pMD. G into 293T cells. Viral particles harvested 48 hours after transfection were used to infect luciferase-expressing UMUC3 and PC3 cells. Positive cells were selected with 2 εg/ml puromycin, and the knockdown of Ral and RalBP1 was confirmed by Western blots. To make stable RalA and RalBP1 expression PC3 cells, flag-tagged wild-type RalA, D49N mutant RalA, RalBP1, and flag vector control were stably transfected into luciferase-expressing PC3 cells. These constructs had silent mismatch mutations that allowed them be expressed despite the presence of shRNA. After 1 mg/ml G418 selection, pools of positive cells were confirmed with Western blots.
PC3 cells were transfected with siRNA oligo duplexes to transiently knockdown of RalA, RalBP1, Sec5, and PLD1. Cells were harvested 72 hours after transfection and counted in a hemacytometer and were resuspended in serum-free Dulbecco modified Eagle medium (DMEM)/F12 medium. Cells (20,000) were added in triplicate to the upper chamber of transwell filters (8.0 εmpores; BectonDickinson, Franklin Lakes, NJ) in a 24-well tissue culture plate. The lower chambers contained 0.65 ml of DMEM/F12 with 2% FBS. For plating control, same number of cells was added in duplicate in wells of 96-well plate (roughly the same growth area compared with the upper chamber of transwell) containing 50 εl of DMEM/F12 medium with 2% FBS. After 20 hours of plating, cells remaining on the upper surface of the filters were removed with cotton swabs, and cells on the lower surface were fixed with 100% methanol, stained with crystal violet, and counted with the aid of a grid coverslip (Bellco Biotechnology, Vineland, NJ). Cell numbers in plating control wells of 96-well plates were determined with CyQuant (Invitrogen) according to manufacturer's instructions.
Real-time reverse transcription-polymerase chain reaction (RT-PCR) was carried out on iCycler Optical Module (Bio-Rad, Hercules, CA) with IQ SYBR Green (Bio-Rad) fluorescent dye included in the PCR to determine the amount of messenger RNA (mRNA) level for RalA, RalBP1, Sec5, and PLD1. Primers used for RalA were forward 5′-CAGACAGCTATCGGAAGAAG-3′ and reverse 5′-AGAAAACACAGAGGAACCCC-3′. Primers for RalBP1 were forward 5′-ACCGGGAGGAGTCTACAAAC-3′ and reverse 5′-CCTCTTCAAATCTGGGCATA-3′. Primers for Sec5 were forward 5′-ATGTCCTCACTTGGGTCAT-3′ and reverse 5′-GCTCGCTCACGCCATCCAG-3′. Primers for PLD1 were forward 5′-CAGAGCTTGGTAATCAGTGG-3′ and reverse 5′-GCGGTCATTTATGTTGGCAG-3′. Real-time RT-PCR for glucuronidase β (Gusb) was used as an internal control, and primers were forward 5′-CCGACTTCTCTGACAACCGACG-3′ and reverse 5′-AGCCGACAAAATGCCGCAGACG-3′. Expression levels of RalA, RalBP1, Sec5, and PLD1 were normalized to the expression of glucuronidase β in each sample by calculating the ratio of individual gene expression to Gusb expression.
Protein extracts were prepared from stable Ral- and RalBP1-depleted UMUC3 and PC3 cells. Western blots were carried out as described  using commercial chemiluminescence reagents (SuperSignalWest Femto; Pierce, Rockford, IL) and a-imaging (Alpha Innotech, San Leandro, CA). The anti-RalA monoclonal antibody was purchased from BD Transduction Laboratory (Franklin Lakes, NJ) and used at 1:1000 dilution. The polyclonal anti-RalBP1 (Abnova, Walnut, CA) antibody was used at 1:2000 dilution. The monoclonal anti-α-tubulin antibody was purchased from Oncogene (San Diego, CA) and was diluted at 1:2000. The HRP-conjugated antimouse and antirabbit secondary antibodies were both from Pierce and were used at 1:2500 dilution. The Rac1 and Cdc42 activation assays were carried out according to the manufacturer's protocol (Upstate, Charlottesville, VA).
Five- to six-week-old male (for PC3 cell) or female (for UMUC3 cell) athymic nude mice (Ncr nu/nu) were obtained from the National Cancer Institute (Frederick, MD). Animals were maintained according to the University of Virginia ICUC guidelines. For subcutaneous or orthotopic tumorigenesis assays, 106 PC3 cells in 50 εl of serum-free medium was mixed 1:1 with Matrigel (Becton Dickinson) immediately before injection. UMUC3 cells were suspended in serum-free medium and injected at a concentration of 106 in 0.1 ml. PC3 and UMUC3 cells were injected bilaterally into the subcutaneous flanks, and tumors were evaluated as described . To evaluate the ability of UMUC3 for lung colonization, 4-week-old female mice were injected through the tail vein with 5 x 106 cells suspended in 0.1 ml of serumfree medium and evaluated by Xenogen bioluminescent imaging (Caliper Life Sciences, Hopkinton, MA) as described , and a modification of a quantitative and sensitive DNA PCR metastasis assay was described previously by our laboratory . We modified the assay by use of human chromosome 12p primers and TaqMan probes. The forward primer used for human 12p was 5′-TTCACTTTGGCAAATGTTTATCC-3′ and the reverse primer was 5′-GTGTGGGAAGGGATTAAACC-3′. The TaqMan probe was 5′-(Hex) CCACGCAACCAGGCAA (BHQ1)-3′. To evaluate the ability of PC3 cells for metastasis, 2 x 105 cells in serum-free medium were inoculated in the left ventricle, and bioluminescent in vivo imaging was used to monitor metastasis weekly. At necropsy, gross tumor areas were dissected en bloc and imaged with high-resolution computed tomography (CT) to examine the bone structure.
CT imaging of dissected tissues was performed on an open-barrel type gantry consisting of two 38-in steel wheels connected by aluminum profile pieces. The CTsubsystem built for small animal imaging consists of a SourceBlock SB-80-1k x-ray source (Source Ray, Bohemia, NY) and a Hamamatsu C7940DP-03CMOS flat panel image sensor. It was operated in 2 x 2 binning mode, resulting ina 1120 x 1172detector element matrix with a 100-εm pitch. Image acquisition and gantry rotation are controlled by a custom-written LabView program. A CT scan consisted of 400 projections, evenly spaced at 0.5-degree increments over 200 degrees. This arrangement results in a total acquisition time of approximately 5 minutes. These images were preprocessed using a custom-written IDL program and reconstructed with a Feldkamp back-projection algorithm (COBRA; Exxim, Inc, Pleasanton, CA).
Data shown are representative of two or more experiments carried out independently. Treatment group comparisons were evaluated using Student's t test. Error bars are SD of the means. Values of P < .05 were taken as a significant difference between means.
Previous studies indicated RalA but not RalB was essential for prostate cancer migration and metastasis to bone . To begin determining which RalA effector was likely involved in this process, we examined the role of RalA as well as their known effectors RalBP1, Sec5, and PLD1 in cell migration. Because Sec5 and Exo84 were both components within exocyst complex, here we only evaluated Sec5. Transient transfection of siRNA duplex specifically targeting RalA, RalBP1, Sec5, and PLD1 decreased their mRNA expression level approximately 60% to 80% (Figure 1A) as evaluated by real-time RT-PCR, which was chosen in lieu of Western analysis in view of its superior quantitative reporting ability and ability to carry out comparison across several targets obviating confounders such as antibody affinity. All knockdowns were associated with a decrease in migration (Figure 1B), whereas no effect on cell number was observed for any siRNAs during the time course of the migration assay. Interestingly, depletion of RalA or RalBP1 inhibited cell migration ~60%, whereas only ~20% inhibition of cell migration was observed with Sec5 and PLD1 reduction despite similar decreased RNA levels. Because RalBP1 knockdown inhibited cell migration to a similar extent as RalA depletion, we concluded that this effector may be an important contributor to RalA-mediated in vivo metastasis.
The similar effect of RalA and RalBP1 knockdown on cell migration prompted us to examine the consequences of such changes on PC3 tumor growth and metastasis. To do this, we created PC3 cells with stable knockdown of RalA and RalB (Figure 1C). Whereas depletion of either Ral protein alone had no effect on in vitro cell growth consistent with previous data , depletion of both RalA and RalB knockdown showed inhibition on subcutaneous tumor growth (Figure 1D). In contrast, RalA but not RalB significantly decreased PC3 cell bone metastasis consistent with previous reports  (Figure 1E).
We also evaluated the effect of RalBP1 depletion on in vivo tumor growth and metastasis. The protein level of RalBP1 was decreased by ~90% after the infection of two lentiviral-based shRNA constructs targeting RalBP1, sh-RalBP1-1, and sh-RalBP1-3 in PC3 cells (Figure 2A). Decreased expression of RalBP1 had no effect on in vitro cell growth (Figure 2Bi). In contrast to in vitro growth in monolayer cultures, depletion of RalBP1 significantly inhibited in vitro tumor growth after subcutaneous inoculation (Figure 2Bii). To assess the requirement for RalBP1 expression for PC3 metastasis, we inoculated cells infected with control and targeting shRNA constructs into the left ventricle of mice. All mice injected with nontarget shRNA cells developed metastasis 9 weeks after injection with most Xenogen signal concentrated in limbs, axial skeleton, and mandibles (Figure 2C). On necropsy, visible tumors were seen on mousemandibles (Figure 2D), and high-resolution CT on dissected mandibles further confirmed the osteolytic destruction typically produced by PC3 metastases (Figure 2E). In contrast, none of the mice injected with RalBP1-1 shRNA-infected cells developed metastatic tumor in the 9-week period, and only one mouse showed metastasis after injection with sh-RalBP1-3 infected cells (Figure 2C).
To determine the requirement for RalBP1 expression for orthotopic prostate cancer cell tumor growth and spontaneous metastasis, we inoculated PC3 cells into mouse prostate. Depletion of RalBP1 by lentiviral-based shRNA construct, sh-RalBP1-1, reduced orthotopic tumor growth as measured by Xenogen bioluminescent signal (Figure 2F). This imaging monitored the inhibition of orthotopic tumor growth as confirmed by actual measurement of the tumor at necropsy (Figure 2G). Mice injected with nontarget shRNA cells developed liver (33%), kidney (67%), and adrenal gland (50%) metastasis at the time of necropsy (Table 1). In contrast, none of the mice inoculated with RalBP1-1-targeting shRNA cells developed metastasis in these organs, indicating that RalBP1 was necessary for PC3 cell spontaneous metastasis from orthotopic tumors.
The necessary role of RalBP1 in local tumor growth and metastasis led us to determine whether RalBP1 overexpression was sufficient to further drive tumor growth and metastasis of PC3 cells. Stable RalBP1 overexpression in PC3 cells (Figure 3A) had no effect on in vitro cell growth (Figure 3B) or in vivo subcutaneous tumor growth (Figure 3C) and metastasis (Figure 3D), suggesting that overexpression of RalBP1 alone was not sufficient to further promote these characteristics in prostate cancer PC3 cells.
Because depletion of RalBP1 had a similar effect on in vivo tumor growth and metastasis of PC3 as RalA knockdown and the former is an effector of the latter, we sought to determine the importance of RalA to RalBP1 binding in regulating PC3 metastasis. To do this, we took advantage of the D49N mutant of RalA that lost most of its binding to RalBP1 (Figure 4A), and the wild-type counterpart both made shRNA-resistant as described in Materials andMethods. These vectors were then transfected into PC3 cells stably expressing either non-target shRNA or RalA shRNA the latter with stably reduced RalA (Figure 4B). Western blot for total cellular RalA showed similar expression levels between shRNA-resistant wild-type and D49N mutant RalA in both nontarget and RalA shRNA-transfected cells (Figure 4B). Overexpression of wild-type or D49N mutant RalA itself had no effect on PC3 in vivo metastasis after intracardic inoculation of nontarget transfected cells (Figure 4C). Depletion of RalA significantly decreased metastasis (Figure 4C) as we saw before (Figure 1E). Both wild-type and D49N mutant RalA were able to rescue the effect of RalA depletion on PC3 metastasis to a similar degree, suggesting that RalBP1 was not the primary downstream effector of RalA in maintaining metastatic competence of PC3. Supporting the notion that RalA and RalBP1 depletion may act on a different set of regulators of metastasis, such depletions led to different effects on Rac1 and Cdc42 activity. Whereas RalBP1 knockdown had no effect on Cdc42 levels or activity but increased Rac1 activity, depletion of RalA decreased both total and active Cdc42 levels and did not have a significant effect on Rac1 activity (Figure 4D).
The observed effect of RalBP1 on prostate cancer cell tumor growth and metastasis led us to ask if the expression of this protein is also necessary for tumor growth and metastasis in other tumor types such as bladder cancer, where RalA expression is associated with higher tumor stage . Bladder cancer is particularly relevant because RalBP1 is highly expressed in this tumor type at both protein  and RNA levels . We stably depleted RalBP1 in the bladder cancer UMUC3 cell line with lentiviral-based shRNA construct targeting RalBP1 (Figure 5A). Depletion of RalBP1 in UMUC3 cells had no effect on their in vitro growth (Figure 5B). After subcutaneous inoculation, tumors from RalBP1 knockdown UMUC3 cells displayed slower growth rate compared with that from nontarget shRNA UMUC3 cells, but this difference did not reach statistical significance (Figure 5C). Because the major metastatic site of bladder cancer is lung, we used the lung colonization assay following the tail vein inoculation of UMUC3 cells to study the role of RalBP1 in bladder cancer metastasis. Depletion of RalBP1 in UMUC3 cells decreased metastasis as assessed by Xenogen bioluminescent signal 6 weeks on both chest area and whole body area (Figure 5D). This finding was confirmed at necropsy using PCR for human-specific DNA in mouse lungs (Figure 5E).
Ral proteins were initially identified as Ras effectors of transformation. However, more recent studies have demonstrated their essential role in cancer migration and metastasis. In prostate cancer, RalA seems to be necessary for these processes , and the expression of this protein is associated with human tumor progression . Here, we evaluated common RalA effectors for their role in human prostate cancer cell migration and identified RalBP1 as an important mediator of this process. However, whereas siRNA-mediated knockdown of Sec5 and PLD1 inhibited cell migration to a lesser extent compared with that of RalBP1 depletion, we cannot exclude them from a role in Ral-mediated cancer metastasis because cell migration is only one aspect determining the metastatic potential of cancer cells.
The similar inhibition of cell migration by RalA and RalBP1 depletion prompted us to further characterize the function of RalBP1 in RalA-dependent metastasis. Although RalBP1 depletion significantly inhibited the in vivo growth and metastasis of PC3 prostate cancer cells, further expression of this protein in PC3 had no effect on these phenotypes. This finding corroborated that of Awasthi et al.  who found that RalBP1 was essential for subcutaneous tumor growth of B16 melanomas, H358 and H520 non-small cell lung cancer, and SW480 colon carcinomas. Such findings were analogous to those with RalA that was found to be necessary for PC3 metastasis but was not sufficient to make them more aggressive . This could occur if both RalA and RalBP1 are in excess compared with a limiting effector. RalBP1 depletion inhibited subcutaneous tumor growth of PC3 but not UMUC3 cells. This may be due to the different effects of this GTPase in different tumor types or may be a cell-specific effect. Similarly, RalA knockdown inhibited subcutaneous growth and metastasis of PC3, although a previous study showed that RalA depletion only had an effect on metastasis . This discrepancy could be due to the different cell numbers injected. The possibility that the decrease in metastasis could result fromdecreased tumor growth at the primary (orthotopic) site also exists, and to determine this, one would need to compare tumors of the same size in the orthotopic location and use this to interpret the metastatic load. Our tail vein injection does not address this issue because this is a measure of metastatic colonization.
Perhaps most provocatively, although RalBP1 and RalA depletion had a similar effect on tumor growth and metastasis in PC3, the result with the D49N mutant RalA experiment pointed out that binding of RalA to RalBP1 is not required for the RalA's effect in PC3 cell metastasis. We also noted that PC3 cells with chronically depleted RalA that were transfected with wild-typeRalA did not recover their full metastatic potential, although the total RalA levels were similar to cells before depletion. This finding may have several explanations. The first is that the RalA shRNA is still exerting an off-target effect on the PC3 cells that is affecting their metastatic competence without affecting other RalA-mediated in vitro phenotypes such as migration. Another possibility is that chronic depletion of RalA has selected cells that are not as susceptible to promotion of themetastatic phenotype by RalA overexpression. Finally, the FLAG tag may not produce a functionally equivalent RalA protein at least for themetastatic phenotype we are evaluating here.
Nevertheless, the conclusion that RalBP1 is not required for the RalA's effect on PC3 cell metastasis is further supported by the different effects of RalA and RalBP1 depletion on Rac1 and Cdc42 activity. Depletion of RalBP1 increased activity of Rac1 consistent with a previous study showing that RalBP1 has a GTPase-activating (GAP) activity toward Rac1 . In contrast, RalA depletion had no effect on Rac1 activity while significantly decreasing both total and active Cdc42 levels, indicating that the effect of RalA knockdown on these proteins may not be completely mediated through RalBP1. Suppression of TBK1 or RalB has recently been shown to lead to selective lethality in KRAS-dependent cell lines , consistent with a previous work linking RalB through Sec5 to TBK1 activation in the setting of tumor cell survival . Hence, paralog-specific effector use may be a possibility that can also explain our findings.
In contrast to these findings, there is evidence that RalBP1 is involved in transformation and tumorigenesis mediated by Aurora-A phosphorylation of RalA at S194 . In addition, both Ral and RalBP1 play important roles in ligand-mediated receptor endocytosis, and the interaction between RalA and RalBP1 and their subsequent translocation to membrane was essential for this process . Whereas the dependence of these processes on the Ral-RalBP1 interaction could potentially affect in vivo cancer cell growth and metastasis, itmay not be operative in all cases or systems (PC3) or tumor histologies (prostate).
In summary, our studies are the first to demonstrate an essential role of RalBP1 in spontaneous and experimental metastasis. This lays the foundation of RalBP1 as a promising target for therapy for this deadly form of cancer.
The authors thank Kathleen Kelly and Gelovani Tjuvajev for the SFGnesTGL vector andMark B. Williams for suggestions with animal imaging.
1This work was supported by a National Institutes of Health award CA104106 to D.T. The authors declare that they have no competing financial interests.