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HO-1 upregulation leads to less fibrotic and extracellular matrix gene expression as well as TGF-β protein production.
Relative mRNA expression (average ± s.e.m.) of PAI-1 (panel A), collagen types I and III (panels B and C), fibronectin (panel D) and TGF-β (panel E). Samples were obtained from obstructed kidneys of UUO and Hemin-treated groups. Gene expression from control animals was assigned a value of 1. * p<0,05 vs. UUO. (F) TGF-β protein, assessed by ELISA assay, in obstructed kidney samples from UUO and Hemin-treated animals. The amount of TGF-β quantified was divided by total tissue protein. * p<0,05 vs. Control and Hemin.