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J Membr Biol. Author manuscript; available in PMC 2010 December 9.
Published in final edited form as:
PMCID: PMC2999834
EMSID: UKMS33624

Measuring the Osmotic Water Permeability of the Plant Protoplast Plasma Membrane: Implication of the Nonosmotic Volume

Abstract

Starting from the original theoretical descriptions of osmotically induced water volume flow in membrane systems, a convenient procedure to determine the osmotic water permeability coefficient (Pos) and the relative nonosmotic volume (β) of individual protoplasts is presented. Measurements performed on protoplasts prepared from pollen grains and pollen tubes of Lilium longiflorum cv. Thunb. and from mesophyll cells of Nicotiana tabacum L. and Arabidopsis thaliana revealed low values for the osmotic water permeability coefficient in the range 5–20 μm · s−1 with significant differences in Pos, depending on whether β is considered or not. The value of β was determined using two different methods: by interpolation from Boyle-van’t Hoff plots or by fitting a solution of the theoretical equation for water volume flow to the whole volume transients measured during osmotic swelling. The values determined with the second method were less affected by the heterogeneity of the protoplast samples and were around 30% of the respective isoosmotic protoplast volume. It is therefore important to consider nonosmotic volume in the calculation of Pos as plant protoplasts behave as nonideal osmometers.

Keywords: Nonosmotic volume, Osmotic swelling, Pollen, Water permeability coefficient, Water transport

Introduction

Since the discovery of the first aquaporins (AQPs) in the 1980s (Denker et al., 1988), the number of identified AQPs has increased almost exponentially due to progress in molecular biology techniques (Quigley et al., 2001). AQP sequences from plants have been reported too, and their water transport activity was demonstrated by the usual functionality test developed for animal AQPs (Zhang, Logee & Verkman, 1990; Zhang & Verkman, 1991; Preston et al., 1992), e.g., expression of the AQP mRNA in Xenopus laevis oocytes (Maurel et al., 1993; Daniels, Mirkov & Chrispeels, 1994; Kamerloher et al., 1994).

Due to the growing interest in the identification and physiological characterization of plant AQPs in their natural environment, protoplast swell assays were performed similar to the oocyte swell assay to determine the value of the water permeability coefficient (Pos) of the plasma membrane. Besides molecular biological experiments (overexpression, knockout), Pos and Arrhenius activation energy are among the few experimentally measurable, physical parameters that indicate the contribution of AQPs to water transport, thus playing an important role in the debate about the physiological function of specific AQPs in plant cells and their regulation (Chaumont, Moshelion & Daniels, 2005; Hill, Shacher-Hill & Shacher-Hill, 2004). Therefore, a precise determination of the Pos value is necessary.

For plant cells, mainly two strategies to determine Pos were used: (1) volume changes of membrane vesicles or of whole protoplasts were recorded until a steady state was reached, and the whole transient, Vt, was used to fit the experimental data to a theoretical function or approximated forms of it (membrane vesicles: Niemietz & Tyerman, 1997; Maurel et al., 1997; protoplasts: Suga et al., 2003; Moshelion, Moran & Chaumont, 2004); (2) measurements on isolated protoplasts were performed over short time periods, assuming that during the initial phase of osmotic swelling the volume changes are linear (“initial rate” approach), and Pos was calculated according to Zhang et al. (1990).

In most reports (see references in Table 1), Pos of individual protoplasts was determined by the second method, essentially similar to that used for Xenopus oocytes, assuming an instantaneous exchange of bath solutions of different osmolalities and a neglectable nonosmotic volume, meaning that the total volume of the protoplast is osmotically active. A problem with the first assumption is the effect of the unstirred layers (Dainty, 1963), which introduces a temporal delay in the exchange of solutions with different osmolalities. This effect depends on the cell size and is not significant for oocytes but could “mask” or bias the initial phase of the volume change of plant protoplasts, leading to underestimation of Pos (Ramahaleo et al., 1999; Moshelion et al., 2004). On the other hand, there is no general agreement about the optimal duration of the measurements, which also depends on the cell properties (e.g., size, water permeability) and on the particular experimental conditions (e.g., magnitude of the osmotic step). For instance, the swelling of oocytes takes a couple of minutes, which enables the sampling of sufficient experimental data points for a reliable linear plot of the volume vs. time. Plant protoplasts are usually smaller and often have higher Pos values than oocytes; therefore, they swell much faster and the duration of the linear volume increase is just several seconds. Additionally, to our knowledge, the nonosmotic volume of plant protoplasts (as well as that of oocytes) has never been considered in calculations of Pos. However, the theoretical function used to describe the time course of water volume fluxes, and thus for calculating Pos, was initially developed by Kedem & Katchalsky (1958) starting from “a thermodynamic description of non-equilibrium systems in which two solutions of the same solvent and solute are separated by a membrane.” The solution of this equation considers only the osmotically active volume of the two compartments, whereas the total measured volume of a cell, especially in plant cell protoplasts, consists of the osmotic and nonosmotic volumes (Wiest & Steponkus, 1978; Dowgert & Steponkus, 1983).

Table 1
Osmotic water permeability coefficient (Pos) values for different plant tissues. Note that the calculation method predominantly used is the initial rate method

Following the reflections above, there is a need for a new, accurate method to calculate the water permeability of plant protoplasts considering their specific properties, e.g., size and nonosmotic volume.

In this study, starting from the original theoretical descriptions of osmotically induced water flow across a membrane system (Kedem & Katchalski, 1958; Dainty, 1963), a convenient procedure to calculate simultaneously both Pos and the nonosmotic volume of individual protoplasts from the time course of their total volume change is presented and the effect of the nonosmotic volume in the calculation of Pos is discussed.

Materials and Methods

Isolation of Plant Protoplasts

Protoplasts from pollen grains and tubes from Lilium longiflorum Thunb. were isolated as described previously (Griessner & Obermeyer, 2003). For preparing mesophyll protoplasts, upper leaves from healthy greenhouse Arabidopsis thaliana and Nicotiana tabacum L. plants were used. Strips, 1 mm wide, were cut and vacuum-infiltrated with isolation medium containing 10 mM KCl, 1 mM CaCl2, 10 mM morpholinoethanesulfonic acid and 500 mM mannitol adjusted to pH 5.5 with tris(hydroxymethyl)aminomethane (TRIS). The strips were then incubated for up to 2 h at room temperature in a medium of the same composition as above, containing cell wall-digesting enzymes: cellulase (1%, w/v) and pectinase (0.02%, w/v). The protoplasts were released by gently shaking the tissue strips after replacing the digestion medium with cold isolation medium adjusted to pH 6 and collected manually by aspiration with a Pasteur pipette. They were subsequently washed three times by centrifugation (80 × g, 5 min) in isolation medium and kept on ice. Only protoplasts of a spherical shape with randomly distributed chloroplasts and cytoplasmic streaming were used for measurements.

Determination of Nonosmotic Volume by Boyle-van’t Hoff Plots

Lily pollen grain and pollen tube protoplasts were incubated for 20–30 min at room temperature in isolation medium (Griessner & Obermeyer, 2003) with osmolalities in the range 288–1,483 mOsm adjusted with mannitol. For each osmolality value, the diameter of 15–80 protoplasts was measured with an inverted microscope equipped with a video system and the corresponding volume was calculated assuming that the protoplasts had a spherical shape. For monitoring the equilibrium volume of mesophyll protoplasts, aliquots of each protoplast sample were resuspended in the isolation medium with various amounts of mannitol, giving an osmolality range of 240–1,280 mOsm. After 20–30 min, the diameters of 12–113 protoplasts were measured for each osmolality value. A freezing point osmometer (OSMOMAT 030; Gonotec, Berlin, Germany) was used to measure the osmolality of the media. The average protoplast volumes were plotted vs. the corresponding reciprocal of the external osmolalities. A straight line was fitted to the data points using the least-square fit method (SigmaPlot; Jandel Scientific, Erkrath, Germany, Corte Madera, CA), and the nonosmotic volume was taken as the intercept of the regression line with the volume axis.

Swelling Experiments and Image Acquisition

Transient volume changes of single protoplasts exposed to hypoosmolar solutions were monitored in a measuring chamber mounted on the stage of an inverted microscope (Axiovert 135; Zeiss, Oberkochen, Germany) equipped with a video camera (CCD-IRIS; Sony, Tokyo, Japan) and a Zeiss 40 × 0.6 Achroplan objective lens. Two identical measuring chambers with a volume of 500 μl each were mounted in parallel, adjacent to each other on the stage of an inverted microscope. One chamber contained a protoplast suspension in isoosmotic medium and the other, a hypoosmotic solution. After a single protoplast was chosen and its initial diameter (D0) was measured, it was transferred with a microcapillary (200–250 μm tip diameter, nanoliter injector; WPI, Berlin, Germany) into the other chamber containing the hypoosmotic solution. Before releasing the protoplast, the microscope was focused on the tip of the microcapillary near the bottom of the chamber so that the subsequent focusing of the protoplast itself could proceed immediately. Image capture was started simultaneously by releasing the protoplast into the hypotonic solution. The osmolality change in the hypotonic medium was negligible since the amount of isotonic medium released with the protoplast was less than 500 nl. In order to insure that the protoplast kept its spherical symmetry during the measurements, the ratio of two perpendicular diameters was measured at the beginning and at the end of the experiment. Only protoplasts with a constant ratio of the diameters were accepted for analysis. All experiments were performed at room temperature.

Images were acquired every 3 s and digitized using a frame grabber (MV Sigma; Matrix Vision, Oppenweiler, Germany). For each protoplast, 150 frames were recorded, resulting in a volume transient of 450 s. The focal plane was manually adjusted if necessary. The 150 frames corresponding to a swelling protoplast were merged into a single file and analyzed using Image Compact 4.0 software (Matrix Vision), which allows measurement of cell diameter (D). The brightness and contrast of the digitized two-dimensional images were adjusted off-line in order to increase the sharpness of the protoplast contour. The scaling factor was 3 pixels · μm−1; thus, the precision of the measurements was 0.33 μm. The corresponding protoplast volumes were calculated as V = (π D3)/6.

Calculation of Pos and β from Single-Cell Volume Transients

In order to calculate Pos, the following data-processing steps were applied. First, the value of the relative nonosmotic volume, β, was calculated from relation (10) (see below). Vfmeasured is the value where the volume relaxation curve reaches a steady maximum. Calculation of β allowed the transformation of the measured volume into the osmotic volume using relation (9). Subsequently, the calculated values of the osmotic volume were fitted with Eq. 4 and the regression parameter k was used to calculate the osmotic water permeability coefficient Pos with relation (5).

Statistical comparisons between different samples were performed using Student’s t-test, whereby a value of p < 0.05 was considered statistically significant. All data transformation, fitting routines and statistical analyses were performed using SigmaPlot version 8.0 software.

Results

Theory

Basic theoretical assumptions

When protoplasts behave as linear osmometers within a given range of osmolalities, the following relation (Boyle-van’t Hoff) is fulfilled:

Π=(nRT)(Vb)=RTc

which is equivalent to

cVos=n

where Π is the osmotic pressure; n and c are the mole number and the concentration of the impermeant solutes in the cell, respectively; and V is the protoplast volume, which consists of an osmotically responsive part (Vos) and an osmotically inactive part, the nonosmotic volume (b).

V=Vos+b

It is further assumed that at equilibrium there is no difference in osmotic as well as hydrostatic pressures across the plasma membrane and that the protoplasts have a spherical shape. According to Kedem & Katchalsky (1958), the bulk flow of water across the plasma membrane of a protoplast suddenly transferred from a solution c0 to a solution with an osmolality cfc0 can be described by the following equation:

dVos(t)dt=PosVwA[cfc(t)]
(1)

with c0Vos0 = c(t)Vos(t) = cfVosf. Vos(t) and c(t) are the time-dependent values of the osmotic volume and internal osmolality, respectively; c0 and V0os are the protoplast initial (t = 0) internal osmolality and osmotic volume, respectively; Vfos is the final (equilibrium) osmotic volume, A is the surface area at t = 0 and Vw is the molar volume of water (18 cm3 · mol−1). It should be kept in mind that in the original theoretical derivation of Eq. 1 (Kedem & Katchalsky, 1958) the volume flux of water across a surface or membrane area A, which in a first approximation was taken to be constant (Dainty, 1963), is described. Using Eq. 1 for the description of volume changes of protoplasts upon hypoosmolar shock therefore presupposes a constant water permeability coefficient and a constant surface area that contribute to the water flux. However, the total surface area of a protoplast will change during the volume increase; but with the assumption that the initial surface area contributing to the water flux (“osmotic active surface”)1 stays constant or that the fraction of the “new” surface area contributes much less to the water flux (in other words, that the osmotic permeability coefficient Pos is constant during the osmotic swelling and, thus, independent from the increase in surface), Eq. 1 can still be used for the description of volume transients of protoplasts and, thus, for the calculation of Pos (see also Discussion).

Rearranging the above equation results in the following differential equation for Vos(t):

dVos(t)d(t)=PosVWAcf[1VfosVos(t)]
(2)

This equation can be integrated, giving the following expression:

InVos(t)VfosV0osVfos+Vos(t)V0osVfos=PosVWAV0oscf2c0t
(3)

During the last three decades, several equivalent forms of Eq. 3 have been reported (Terwilliger & Solomon, 1981; Mlekoday, Moore & Levitt, 1983; van Heeswijk & van Os, 1986; Olbrich et al., 2000).

In order to simplify the mathematical expressions, we introduced the following notations:

c0cf=VfosV0os=α

Vos(t)V0os=Vrelos(t)

k=PosVWAcfαV0os

Eq. 3 now becomes

InαVrelos(t)α1+Vrelos(t)1α=kt
(4)

By fitting experimental values of Vrelos(t) and t with Eq. 4, the parameter k can be determined and Pos can be calculated as follows:

Pos=kαV0osVWAcf
(5)

A single exponential function is not appropriate for calculating correct Pos values

Equation 4 obviously does not have a simple mathematical form; therefore, it would be reasonable to look for an “easier to manage” equivalent expression. For this purpose, a function of the “exponential rise to maximum” type would seem to be a good candidate:

Vrelos(t)=α(α1)eKt
(6)

This phenomenological function fits well the experimental data from a typical osmotic swelling experiment; however, it has to be clarified how the time constant K in function (6) relates to k in equation (4), and eventually one should be able to interpret K in terms of Pos. As an approximated form of Eq. 4, the following function was proposed (van Heeswijk & van Os, 1986), which is practically equivalent to the phenomenological function (6):

InαVrelosα1=Kt
(7)

with K = αk (k and α as previously defined; van Heeswijk & van Os, 1986, denoted K as ke). Consequently, Pos was calculated as follows:

Pos=KV0osVWAcf
(8)

However, the following example demonstrates that although function (7) can be used to fit experimental data, the calculated water permeability coefficient deviates considerably from the true value. In Figure 1, data points corresponding to a hypothetical protoplast with a diameter of 40 μm and a Pos of 14.9 μm · s−1 exposed to an osmolality change from 600 to 300 mOsm were generated with Eq. 4. The corresponding rate constant for this simulation was k = 0.006. The simple exponential function (6) was fitted to these data, and the value obtained for the fit parameter K was 0.0093, giving for Pos a value of 23.13 μm · s−1, which means an overestimation of the true Pos by >50%. Supposing now the rate constant is defined as K = αk (van Heeswick & van Os, 1986), k takes the value 0.00465 and consequently Pos (calculated with relation [8]) is 11.56 μm · s−1, a value that underestimates the true Pos by 23%. Function (7) proves thus to be a poor approximation for function (4), and calculation of Pos with relation (8), on the basis of a k value derived from function (7), can lead to erroneous values.

Fig. 1
Single exponential functions do not describe adequately the osmotic swelling data. The plot shows the first 200 s from a simulated 800 s volume transient. The entire transient is shown in the inset. Continuous line represents data simulated with theoretical ...

To sum up, although functions (6) and (7) describe roughly the experimental data, there is no theoretically founded, straightforward relationship between K and Pos as defined in Eq. 1. Therefore, the most accurate method for determining Pos is to fit function (4) to the measured data and finally to calculate Pos with relation (5) using the fit parameter k.

The nonosmotic volume

It is commonly accepted that just a part of the cell volume (the so-called osmotic volume, Vos) responds to an external osmotic challenge and that within a cell there are osmotically inactive components such as cell solids or other cell contents (endomembranes, starch grains, lipid drops, etc.), representing the nonosmotic volume, b. Very frequently, b is normalized to the isoosmotic volume, Viso, giving the fractional nonosmotic volume β (β = b/Viso). The value of β depends on the cell type and on the physiological state of the cell (Dowgert & Steponkus, 1983; Reed et al., 1987). In plant cells and algae, β values range from ca. 8% (nonacclimated rye protoplasts: Dowgert & Steponkus, 1983) to 60–80% (Dunaliella parva: Rabinowitch, Grover & Ginzburg, 1975), whereas in animal cells β values from 14% (J774 macrophages: Echevarria & Verkman, 1992) to 57% (Madin-Darby canine kidney [MDCK] cells: Zelenina & Brismar, 2000) have been reported. In the field of cryopreservation research, the importance of considering nonosmotic volume was recognized very early (see review by Steponkus, 1984). It seems necessary to take β into consideration in protoplast swelling experiments, too, since it is the osmotic volume, Vos, and not the entire cell volume that obeys the theoretical Eq. 1. Therefore, corrections are needed to the measured cell volume in order obtain a correct value for Pos.

Starting from the definition of β and assuming V0 = Viso, a mathematical expression can be found that relates the relative osmotic volume to the relative measured volume:

Vrelos(t)=Vrelmeasured(t)β1β
(9)

with

Vrelmeasured(t)=Vmeasured(t)V0measured

and

Vrelos(t)=Vos(t)V0os

In practice, by introducing β in relation (9), the measured volume data Vrelmeasured can be transformed into osmotic volume data Vrelos, which can then be fitted with function (4), in order to determine the rate constant k and eventually to calculate Pos.

There are two possibilities to infer the value of the nonosmotic volume b (and consequently β) for a certain cell type: (1) the standard procedure of measuring the steady-state volume of the cell at several external osmolalities and interpolate b as the volume at infinite osmolality from the Boyle-van’t Hoff linear plot and (2) monitoring a single transient volume increase upon a hypoosmotic challenge (β > 1) on a time scale which is long enough to allow the cell to reach the final steady-state value Vfmeasured. The following formula can then be used to calculate β:

β=αVfmeasuredV0measuredα1
(10)

Relation (10) is easily derived from relation (9), considering initial and final values for the protoplast volume and using the usual notations. An equivalent expression was used in osmotic water permeability experiments on MDCK cells, giving a β of 57% (Zelenina & Brismar, 2000).

Experimental Determination of Pos and β

After isolation from their respective tissues, the protoplasts were incubated in an appropriate isoosmolar medium and their diameters determined. Usually, the majority of lily pollen grain protoplast diameters ranged from 40–60 μm, with a peak around 80 μm, at an osmolality of ca. 800 mosmol · kg−1. At the same osmolar conditions, the size of pollen tube protoplasts showed a wider distribution of 45–85 μm, peaking at around 60 μm. The peak diameter of the size distribution of mesophyll protoplasts was around 50 μm for N. tabacum (range 45–70 μm) and ca. 60 μm for A. thaliana (range 50–80 μm) in an isoosmolar medium of ca. 450 mosmol · kg−1.

Determination of nonosmotic volume from Boyle-van’t Hoff plots

Incubation of protoplasts under non-isoosmolar conditions resulted in a change of the protoplast volume (Fig. 2). Using the intercepts of the regression lines in the Boyle-van’t Hoff plots (Fig. 2a–d), values for b were determined. For lily pollen grain protoplasts, b was 146,000 μm3, corresponding to a relative nonosmotic volume β of 42%; for lily pollen tube protoplasts, b was 11,600 μm3, corresponding to β of 4.7%. Mesophyll protoplasts showed much lower values for b: for N. tabacum protoplasts, the Boyle-van’t Hoff plot gave a nonosmotic volume of 3,214 μm3, and for A. thaliana protoplasts, b was 643 μm3. The corresponding β values were 1.7% and 0.4%. Note that at lower osmolalities the range covered by the measured volumes was in all samples wider than at higher osmolalities. Consequently, the standard deviations from the mean values were considerably higher at lower osmolalities, limiting the accuracy of the fit with a regression line, from which eventually the value of the nonosmotic volume, b, was extrapolated.

Fig. 2
Boyle-van’t Hoff plots for protoplasts isolated from (a) Lilium longiflorum pollen grains and (b) tubes and protoplasts from mesophyll cells of (c) Nicotiana tabacum and (d) Arabidopsis thaliana show that these protoplasts behave as linear osmometers ...

Simultaneous determination of the osmotic parameters Pos and β

Starting from isoosmotic conditions, single protoplasts were exposed to hypoosmotic solutions that gave α values (α = c0/cf) ranging 1.38–1.89 for N. tabacum, 1.41–1.81 for A. thaliana, 1.7–2.3 for lily pollen grains and 1.88–2.1 for lily pollen tube protoplasts. In order to establish an optimal sampling rate, we adapted the image acquisition speed to the swelling speed of the protoplasts, considering our resolution limits. We noticed that in the most rapid phase of volume change the protoplasts increased their volume by ca. 20% within the first 30–60 s. A 20% volume increase corresponds to a 6.3% increase in protoplast diameter. For a representative protoplast with a diameter of 60 μm, this means an increase of 3.78 μm, which on a digital image gives 10 pixels (our scaling factor was 3 pixels · μm−1). It follows that acquiring one frame every 3 s gives a reasonable sampling rate. A whole volume transient extended over 450 s.

The first step of the data processing was to use the swelling curve for the calculation of the relative nonosmotic volume, β. The raw volume data (Vmeasured) were then transformed into osmotic volume data (Vos), giving a corrected swelling curve which was fitted with Eq. 4 in order to calculate the osmotic water permeability coefficient Pos. Thus, the parameters β and Pos were determined from the volume transient of the same protoplast, reflecting the osmotic properties of this single protoplast. In Figure 3, typical swelling curves (volume transients) are shown for the different protoplast types. Note that the Arabidopsis protoplast in Figure 3d has a lower initial swelling rate than the lily pollen tube protoplast, although their Pos values are practically equal. In addition, the initial swelling rates of the Arabidopsis and the pollen grain protoplasts look very similar, though the Pos value of the pollen grain protoplast (Fig. 3a) is only half that of the Arabidopsis protoplasts (Fig. 3d). These apparent discrepancies can be explained if one writes Eq. 1 for the relative osmotic volume Vrelos at t = 0, which gives the initial protoplast swelling rate:

dVrelosdt(t0)=Pos6D0(1β)VWΔc

where D0 is the initial diameter of the protoplast and Δc = cfc0 is the osmotic step, with the other symbols as described in the theory section. It can be seen that besides the value of the osmotic permeability coefficient Pos, also the initial size of the protoplast, the relative nonosmotic volume β and the height of the osmotic step determine the rate of volume change in the initial phase of the swelling process.

Fig. 3
Typical volume transients measured for protoplasts isolated from lily pollen grains (a), pollen tubes (b), Nicotiana tabacum mesophyll (c) and Arabidopsis thaliana mesophyll (d). Measured data were fitted with the theoretical function (4), and the resulting ...

More than 90% of the protoplasts survived the hypoosmotic challenge and reached an equilibrium size. A small percentage of the protoplasts burst within the first 100 s of the recordings and were not analyzed, whereas analysis of protoplasts that burst shortly before reaching an equilibrium (t = 300–400 s) revealed an abnormally high Pos, usually one order of magnitude higher than in individuals from the same sample and species that did not burst until the end of the measurements. These bursting protoplasts were excluded from further processing; yet, it is interesting to note that measurements over shorter time spans (<100 s) would have most probably included contributions from such protoplasts, which would have considerably broadened the range of the calculated Pos values. Our reason to exclude these protoplasts from further processing was the assumption that the integrity of their plasma membrane was affected at a submicroscopic level undetectable by light microscopy, rather than having higher membrane permeability. In our experiments, the samples were exposed to relatively moderate osmotic stress, which most of the protoplasts survived. Therefore, the bursting protoplasts were rejected and not treated as individuals with high water permeability coefficients of physiological relevance.

The mean values of the osmotic parameters β and Pos for different protoplast preparations are listed in Table 2. For lily pollen grain protoplasts, we obtained Pos values ranging 2.2–10.4 μm · s−1, whereas for pollen tube protoplasts the values were twice as large, 7.6–21 μm · s−1. The difference between these two samples is statistically significant (p < 0.05, Student’s t-test). N. tabacum protoplasts showed values in the range 4.0–13.9 μm · s−1; values obtained for A. thaliana protoplasts extended over a broader range, 3.8–20.6 μm · s−1.

Table 2
Values of the osmotic parameters Pos and β obtained for different protoplast types. The water permeability coefficient was calculated by three methods: (a) fitting the transient osmotic volume with Eq. 4, (b) using the “initial rate” ...

The same experimental data were also used to determine Pos by two other methods: the “initial rate” method and by fitting Eq. 4 to volume data which were not corrected for the nonosmotic volume. Both analysis strategies resulted in Pos values which were lower than those obtained by fitting osmotic volume data to Eq. 4 (Table 2). In addition, a difference between the values of β can be observed depending on the method used to obtain them. β values can be calculated from the single volume transients that were also used to calculate Pos of the same protoplast or by interpolation from the Boyle-van’t Hoff plots, giving lower values for the respective protoplast, except for the pollen grain protoplasts (Table 2).

In Figure 4, the calculated Pos value (Eq. 4) was plotted against the relative increase in the surface area ([SfinalS0]/S0) for all individual protoplasts measured in this study. No significant correlation between Pos and the corresponding surface increase was noticed (r2 values of regression lines <0.5, data not shown) for the different protoplast species, indicating that a large surface increase is not caused by a high Pos. On the other hand, a positive correlation of Pos with surface enlargement would indicate an active contribution of the new membrane area to the water transport, e.g., by incorporation of AQPs into the plasma membrane during swelling. Therefore, the data of Figure 4 do not contradict the prerequisite of a constant water permeability coefficient of the Kedem-Katchalsky model or our assumption of a constant “osmotically active” surface.

Fig. 4
Scatter plot of individual Pos values of all four protoplast types were plotted against the respective relative surface increase. Each data point in the plot represents a single protoplast, with Pos and ΔS/S0 calculated from its volume transient. ...

Discussion

This theoretical and experimental study shows that the accuracy of calculating the protoplast osmotic water permeability Pos can be considerably improved by taking into account the nonosmotic volume and by analyzing the whole swelling curve.

Previous reports on water channels in plant cells used stopped flow measurements on membrane vesicle suspensions (Niemietz & Tyerman, 1997; Maurel et al., 1997), heterologous expression in oocytes (e.g., Maurel et al., 1993; Daniels et al., 1994; Kamerloher et al., 1994; Chaumont et al., 1998) and measurements on whole isolated protoplasts (see Table 1 for references). For the calculation of Pos, most authors have used a method based on the “initial rate” of volume change and reported values that largely ranged 1–500 μm · s−1, depending on the tissue type and age of the plants used. In contrast to experiments that were performed only during the initial linear phase of the volume increase, we used volume transients from the onset of the swelling until a steady state was reached and the volume data were corrected for the contribution of the nonosmotic volume. This is, in our opinion, a necessary step since the theoretical Eq. 1 that describes the time course of the volume change applies only to the osmotic volume (Kedem & Katchalski, 1958).

Using the described approach for the calculation of Pos, the values of the water permeability coefficient of mesophyll protoplasts are within the range common for cells of similar origin (see Table 1 and Table 1 in Chaumont et al., 2005), 5–20 μm · s−1. These rather low values may indicate a lower AQP expression level in leaf mesophyll cells than in other parts of the plant, a lower activity of AQPs or both. The osmotic water permeability coefficient for lily pollen grains showed values in the same range as for mesophyll cells. Interestingly, the values corresponding to the pollen tube protoplasts were twice as high as those for pollen grains, suggesting a possible physiological role of AQPs in water uptake during pollen tube elongation. The data of Table 2 clearly show that the values of the osmotic water permeability coefficient are underestimated when they are calculated with the “initial rate” method or when the nonosmotic volume is ignored. Note that no significant differences in the water permeability coefficient were observed between pollen grains and tubes when neglecting the nonosmotic volume.

The Nonosmotic Volume

We determined the nonosmotic volume of protoplasts by two different methods: (1) by interpolation from linear Boyle-van’t Hoff plots and (2) by fitting whole volume transients measured during osmotic swelling. The values obtained with the first method are lower than those calculated with the second one, except for pollen grain protoplasts. However, when the upper limit of the regression line at 95% confidence is considered in the Boyle-van’t Hoff plots, the discrepancies are less considerable. For the tobacco protoplasts, the intercept of the upper regression line (upper dotted line in Fig. 2) gives a value of 35,000 μm3, which yields a β of 21%, which is not very different from the calculated value using the second method (Table 2). For Arabidopsis protoplasts, the same procedure gives an upper estimate of the nonosmotic volume of 15,000 μm3, indicating a β of 14.4%, also closer to the calculated β value in Table 2. For pollen grain protoplasts, the upper estimate of β gives 52% and for pollen tube protoplasts, 40.3%. To obtain Vos from the measured volume transients (Vmeasured), the β value determined from the volume transient of the same, single protoplast, also used to calculate Pos, was used. These β values are much less affected by the size heterogeneity of the protoplast population as indicated by the standard deviation (graphs in Fig. 2). In addition, the regression line in the Boyle-van’t Hoff plots can be biased by the less reliable value at the lowest medium osmolality, probably leading to underestimated β values. In general, β is thought to be correlated with different cell structures such as organelles, starch grains, hydration water, etc. (Zimmermann, 1978); and β values in the same range have been measured in other organisms, e.g., 40–50% for the freshwater alga Ochromonas malhamensis (Zimmermann, 1978) and 60–80% for the halophilic alga Dunaliella parva (Rabinowitch et al., 1975). Changes in the relative nonosmotic volume are considered to be a possible mechanism of adaptation to water stress. For example, in Platymonas subcordiformis, the absolute value, b, of the nonosmotic volume is reported to be constant over a large range of salinities, β being found to be shifted from 45% at low salinity to 80% at high salinity (see Zimmermann, 1978, and references therein). Furthermore, the osmoregulation in cotton in response to water stress has been correlated to a starch content-induced increase in the cellular nonosmotic volume (Ackerson & Hebert, 1981).

The relative homogeneity in the β values of our samples is not easy to interpret, given the important structural differences between mesophyll protoplasts, which have a large central vacuole with osmotically active solutes, and pollen protoplasts, which lack a central vacuole but contain many starch grains, liposomes and large generative or sperm cells. One would expect that mesophyll cells have a lower β than pollen protoplasts. Nevertheless, for Commelina communis guard cells, where the vacuoles dominate the cell volume, high values for the relative nonosmotic volume, up to 45%, were reported (Weyers & Fitzsimons, 1982) and it was hypothesized that a large proportion of the guard cell β is due to chloroplast starch. Also, algae, although having a vacuole, show large values of the nonosmotic volume (see citations above). One may, therefore, speculate that not only do specific cellular structures contribute to β but β includes all cell properties accounting for the nonideal osmotic behavior of protoplasts (e.g., stiffness of membrane, rigidity of cytoskeleton) as suggested to explain the resistance in the volume reduction of the hardy dogwood parenchymal cells (β = 48%) at high external osmotic pressures (Williams & Williams, 1976). The detection of deviations from the ideal osmometer behavior depends on the chosen experimental conditions, e.g., the height of the osmolality step and the precision in detecting protoplast volume changes. Using the notations introduced here and the Boyle-van’t Hoff law, we remind that for ideal osmometers cfVf = c0V0, whereas for real protoplasts cfVfc0V0 = b(c0 − cf). Let us suppose that a protoplast with a nonosmotic volume b = 0.3V0 (corresponding to β = 30%) experiences a small osmotic step of just 10% (c0 − cf = 0.1c0). The deviation from an ideal osmometer is then c0V0 − cfVf = 0.03c0V0, or, rearranging, cfVf = 0.97 c0V0. This 3% deviation from the expected value of the volume corresponds to a deviation of 1% in the diameter of the protoplast (e.g., 0.5 μm for diameters of 50 μm). This is definitely too small to be detectable in a swell assay under such experimental conditions.

The Osmotic Active Surface

In the Kedem-Katchalsky model, the water permeability coefficient is a constant and, in a first approximation, the assumption of a constant surface area, A, is made (Dainty, 1963). In our study, in particular we suggested an “osmotic active” surface area of the protoplast plasma membrane that does not change during the measurement, although the whole membrane surface area changes along with the volume. The assumption of a constant “osmotic active” surface area is fulfilled if the new incorporated membrane material contributes much less to the water transport properties of the membrane than the already existing membrane, also meaning a constant osmotic permeability coefficient, Pos, during the swelling of the protoplast. Typically, the intrinsic elastic stretching of the protoplast membrane can only account for a 2% increase in surface area (Wolfe, Dowgert & Steponkus, 1986), which is completed in usually <10 s. Further changes in the membrane area occurring during a swelling episode do not conserve the amount of material in the membrane. It is hypothesized that in plant protoplasts the plasma membrane exchanges material with an internal membrane reservoir in order to maintain a certain resting tension (Wolfe et al., 1986) and that this is a general mechanism used by animal and plant cells to regulate their surface area (Raucher & Sheetz, 1999; Morris & Homann, 2001). In guard cell protoplasts, it was shown that osmotically induced changes in the surface area are accomplished by reversible incorporation of membrane material during shrinking or stretching (Shope, DeWald & Mott, 2003), more precisely by fission and fusion, respectively, of vesicles from a preplasma membrane pool (Homann, 1998; Kubitscheck, Homann & Thiel, 2000). The question if the additional membrane material has the same characteristic composition as the actual plasma membrane, in other words if new transporters are incorporated or not during swelling, is still open. Some information concerning ion transporters comes from patch-clamp measurements in the whole-cell configuration. By doing simultaneous capacitance and conductance measurements, it was shown that in guard cell protoplasts K+ channels are added to the plasma membrane during swelling (Hurst et al., 2004), while addition of membrane material in maize coleoptile protoplasts was not accompanied by incorporation of new ion transporters (Thiel, Sutter & Homann, 2000). However, one cannot extrapolate easily these results to the actual situation in intact swelling protoplasts since patched cells were shown to swell considerably more than intact cells do because they are internally dialyzed against the filling solution of the patch pipette (Doroshenko, 1999). There are also reports showing that AQPs cycle between cellular internal membrane compartments during osmotic and salt stress in ice plant (Kirch et al., 2000; Vera-Estrella et al., 2004), and the picture that emerges is that under stress conditions the tonoplast AQPs are redistributed to endosomal compartments, while plasma membrane-located AQPs are not. Owing to its higher water membrane permeability compared to the plasma membrane (e.g., 100 times higher in tobacco suspension cells: Maurel et al., 1997), the vacuole buffers the cytoplasm rapidly against damaging, large volume changes (Tyerman et al., 1999). It is therefore intuitively reasonable to assume that for efficient cell homeostasis, in a hypoosmotic milieu more AQPs would be incorporated in the tonoplast and not in the plasma membrane, even if its area increases. The data of Figure 4 show no correlation between Pos and the increase in membrane area for the individual protoplasts investigated, implying that additional membrane material does not necessarily mean increased water permeability of the membrane. We remind that Pos reflects a whole-membrane property, being actually a measure of the speed of water movement across the plasma membrane (its measuring unit is μm · s−1). Therefore, one might assume that the total amount of active AQPs depends upon the size of Pos and not their density. Considering that the new membrane incorporated during protoplast swelling would have contained additional water transport units (AQPs), Pos would increase continually and proportionally to the surface increase. This has at least two immediate consequences: (1) function (4) fails to fit the experimental data because in the Kedem-Katchalski model Pos is constant and (2) if one still calculates Pos on the basis of the Kedem-Katchalski model despite a “bad fit,” the resulting Pos values would be high for those protoplasts that experienced a high relative surface increase. In other words, one could notice a positive correlation between ΔS/S0 and Pos, which is not seen in Figure 4.

Recently, Moshelion et al. (2004) reported water permeability measurements on whole protoplasts isolated from cultured maize cells and evidenced dynamic changes of plasma membrane water permeability occurring upon swelling. They used a numerical solution of the theoretical equation describing the volume transients and tested several models of surface area increase. For their data, the best fit was achieved by a model in which membrane surface area increase is paralleled by an increase of Pos. We cannot confirm such dynamic changes of Pos since our data on pollen and mesophyll protoplasts could be well fitted by function (4) with the assumption that the osmotic active surface area and the water permeability coefficient stay constant during the swelling.

In conclusion, when experimental protoplast swelling data are fitted by a function based on the Kedem-Katchalsky model, the prerequisites of the model (osmotic active volume and a constant water permeability coefficient) have to be taken into account or at least one has to be aware of its limitations. Considering the nonosmotic volume of plant protoplasts was crucial for correct calculation of Pos in whole protoplast swell assays when using the equation derived by Kedem & Katchalsky (1958). Otherwise, an incorrect calculation of Pos may mask differences with physiological relevance as shown between the water permeability coefficients of pollen grain and tube protoplasts.

Acknowledgements

We are grateful to Prof. Dr. Steve Tyerman (Adelaide University, Australia) and Prof. Dr. Anton Schäffner (GSF-Forschungszentrum für Umwelt und gesundheit, Neuherberg, Germany) for valuable discussions. This work was partially financed by grants from the Austrian Research Foundation FWF-Fond FW Förderung des Wissenschaftlichen Forschung (P 13064, P 17227).

Footnotes

1The term “osmotic active surface” is introduced in accordance to the term “osmotic volume,” although we are aware that a surface area is not osmotically active in a physical sense.

References

  • Ackerson RC, Hebert RR. Osmoregulation in cotton in response to water stress. I. Alterations in photosynthesis, leaf conductance, translocation and ultrastructure. Plant Physiol. 1981;67:487–488. [PubMed]
  • Chaumont F, Barrieu F, Herman EM, Chrispeels MJ. Characterisation of a maize tonoplast aquaporin expressed in zones of cell division and elongation. Plant Physiol. 1998;117:1143–1152. [PubMed]
  • Chaumont F, Moshelion M, Daniels MJ. Regulation of plant aquaporin activity. Biol Cell. 2005;97:749–764. [PubMed]
  • Comparot S, Morillon R, Badot P-M. Water permeability and revolving movement in Phaseolus vulgaris L. twinning shoots. Plant Cell Physiol. 2000;41:114–118. [PubMed]
  • Dainty J. Water relations in plant cells. Adv Bot Res. 1963;1:279–326.
  • Daniels MJ, Mirkov TE, Chrispeels MJ. The plasma membrane of Arabidopsis thaliana contains a mercury-insensitive aquaporin that is a homolog of the tonoplast water channel protein TIP. Plant Physiol. 1994;106:1325–1333. [PubMed]
  • Denker BM, Smith BL, Kuhaida FP, Agre P. Identification, purification and partial characterization of a novel Mr 28,000 integral membrane protein from erythrocytes and renal tubules. J Biol Chem. 1988;263:15634–15642. [PubMed]
  • Ding X, Iwasaki I, Kitagawa Y. Overexpression of a lily PIP1 gene in tobacco increased the osmotic water permeability of leaf cells. Plant Cell Environ. 2004;27:177–186.
  • Doroshenko P. High intracellular chloride delays the activation of the volume-sensitive chloride conductance in mouse L-fibroblasts. J Physiol. 1999;514.2:437–446. [PubMed]
  • Dowgert MF, Steponkus PL. Effect of cold acclimation on intracellular ice formation in isolated protoplasts. Plant Physiol. 1983;72:978–988. [PubMed]
  • Echevarria M, Verkman AS. Optical measurement of osmotic water transport in cultured cells. Role of glucose transporters. J Gen Physiol. 1992;99:573–89. [PMC free article] [PubMed]
  • Griessner M, Obermeyer G. Characterization of whole-cell K+ currents across the plasma membrane of pollen grain and tube protoplasts of Lilium longiflorum. J Membr Biol. 2003;193:99–108. [PubMed]
  • Hill AE, Shachar-Hill B, Shachar-Hill Y. What are aquaporins for? J Membr Biol. 2004;197:1–32. [PubMed]
  • Homann U. Fusion and fission of plasma membrane material accommodates for osmotically induced changes in the surface area of guard-cell protoplasts. Planta. 1998;206:329–333.
  • Hurst AC, Meckel T, Tayefeh S, Thiel G, Homann U. Trafficking of the plant potassium inward rectifier KAT1 in guard cell protoplasts of Vicia faba. Plant J. 2004;37:391–397. [PubMed]
  • Kaldenhoff R, Grote K, Zhu J-J, Zimmermann U. Significance of plasmalemma aquaporins for water transport in Arabidopsis thaliana. Plant J. 1998;14:121–128. [PubMed]
  • Kamerloher W, Fisher U, Piechottka GP, Schäffner AR. Water channels in the plant plasma membrane cloned by immunoselection from a mammalian expression system. Plant J. 1994;6:187–199. [PubMed]
  • Kedem O, Katchalsky A. Thermodynamic analysis of the permeability of biological membranes to non-electrolytes. Biochim Biophys Acta. 1958;27:229–246. [PubMed]
  • Kirch H-H, Vera-Estrella R, Golldack D, Quigley F, Michalowski B, Barkla BJ, Bohnert HJ. Expression of water channel proteins in Mesembryanthemum crystallinum. Plant Physiol. 2000;123:111–124. [PubMed]
  • Kubitscheck U, Homann U, Thiel G. Osmotically evoked shrinking of guard-cell protoplasts causes vesicular retrieval of plasma membrane into the cytoplasm. Planta. 2000;210:423–431. [PubMed]
  • Maurel C, Reizer J, Schroeder JI, Chrispeels MJ. The vacuolar membrane protein γ-TIP creates water specific channels in Xenopus oocytes. EMBO J. 1993;12:2241–2247. [PubMed]
  • Maurel C, Tacnet F, Güclü J, Guern J, Ripoche P. Purified vesicles of tobacco cell vacuolar and plasma membranes exhibit dramatically different water permeability and water channel activity. Proc Natl Acad Sci USA. 1997;94:7103–7108. [PubMed]
  • Mlekoday HJ, Moore R, Levitt DG. Osmotic water permeability of human red cell. Dependence on direction of water flow and cell volume. J Gen Physiol. 1983;81:213–220. [PMC free article] [PubMed]
  • Morillon R, Chrispeels MJ. The role of ABA and the transpiration stream in the regulation of the osmotic water permeability of leaf cells. Proc Natl Acad Sci USA. 2001;98:14138–14143. [PubMed]
  • Morillon R, Lassalles J-P. Water deficit during root development: effects on the growth of roots and on water permeability of isolated root protoplasts. Planta. 2002;214:392–399. [PubMed]
  • Morris CE, Homann U. Cell surface area regulation and membrane tension. J Membr Biol. 2001;179:79–102. [PubMed]
  • Moshelion M, Becker D, Biela A, Uehlein N, Hedrich R, Otto B, Levi H, Moran N, Kaldenhoff R. Plasma membrane aquaporins in the motor cells of Samanea saman: diurnal and circadian regulation. Plant Cell. 2002;14:727–739. [PubMed]
  • Moshelion M, Moran N, Chaumont F. Dynamic changes in the osmotic water permeability of protoplast plasma membrane. Plant Physiol. 2004;135:2301–2317. [PubMed]
  • Niemietz CM, Tyerman SD. Characterization of water channels in wheat root membrane vesicles. Plant Physiol. 1997;115:561–567. [PubMed]
  • Ohshima Y, Iwasaki I, Suga S, Murakami M, Inoue K, Maeshima M. Low aquaporin content and low osmotic water permeability of the plasma and vacuolar membranes of a CAM plant Graptopetalum paraguayense: comparison with radish. Plant Cell Physiol. 2001;42:1119–1129. [PubMed]
  • Olbrich K, Rawicz W, Needham D, Evans E. Water permeability and mechanical strength of polyunsaturated lipid bilayers. Biophys J. 2000;79:321–327. [PubMed]
  • Preston GM, Carroll TP, Guggino WB, Agre P. Appearance of water channels in Xenopus oocytes expressing red cell CHIP28 protein. Science. 1992;256:385–387. [PubMed]
  • Quigley F, Rosenberg JM, Shachar-Hill Y, Bohnert HJ. From genome to function: the Arabidopsis aquaporins. Genom Biol. 2001;3:1–17. [PMC free article] [PubMed]
  • Rabinowitch S, Grover NB, Ginzburg BZ. Cation effects on volume and water permeability in the halophilic algae Dunaliella parva. J Membr Biol. 1975;22:211–230. [PubMed]
  • Ramahaleo T, Morillon R, Alexandre J, Lasalles J-P. Osmotic water permeability of isolated protoplasts. Modifications during development. Plant Physiol. 1999;119:885–896. [PubMed]
  • Raucher D, Sheetz M. Characteristics of a membrane reservoir buffering membrane tension. Biophys J. 1999;77:1992–2002. [PubMed]
  • Reed RH, Chudek JA, Foster R, Gadd GM. Osmotic significance of glycerol accumulation in exponentially growing yeasts. Appl Environ Microbiol. 1987;53:2119–2123. [PMC free article] [PubMed]
  • Shope JC, DeWald DB, Mott KA. Changes in surface area of intact guard cells are correlated with membrane internalization. Plant Physiol. 2003;133:1314–1321. [PubMed]
  • Siefritz F, Biela A, Eckert M, Otto B, Uehlein N, Kaldenhoff R. The tobacco plasma membrane aquaporin NtAQP1. J Exp Bot. 2001;52:1953–1957. [PubMed]
  • Steponkus PL. Role of the plasma membrane in freezing injury and cold acclimation. Annu Rev Plant Physiol. 1984;35:543–584.
  • Suga S, Murai M, Kuwagata T, Maeshima M. Differences in aquaporin levels among cell types of radish and measurement of osmotic water permeability of individual protoplasts. Plant Cell Physiol. 2003;44:277–286. [PubMed]
  • Terwilliger TC, Solomon AK. Osmotic water permeability of human red cells. J Gen Physiol. 1981;77:549–570. [PMC free article] [PubMed]
  • Thiel G, Sutter J-U, Homann U. Ca2+-sensitive and Ca2+-insensitive exocytosis in maize coleotile protoplasts. Pflugers Arch. 2000;439:R152–R153. [PubMed]
  • Tyerman SD, Bohnert HJ, Maurel C, Steudle E, Smith JAC. Plant aquaporins: their molecular biology, biophysics and significance for plant water relations. J Exp Bot. 1999;58:1055–1071.
  • van Heeswijk MPE, van Os CH. Osmotic water permeabilities of brush border and basolateral membrane vesicles from rat renal cortex and small intestine. J Membr Biol. 1986;92:183–193. [PubMed]
  • Vera-Estrella R, Barkla BJ, Bohnert HJ, Pantoja O. Novel regulation of aquaporins during osmotic stress. Plant Physiol. 2004;135:2318–2329. [PubMed]
  • Weyers JDB, Fitzsimons PJ. The nonosmotic volume of Commelina guard cells. Plant Cell Environ. 1982;5:417–421.
  • Wiest SC, Steponkus PL. Freeze-thaw injury to isolated spinach protoplasts and its simulation at above freezing temperatures. Plant Physiol. 1978;62:699–705. [PubMed]
  • Williams JM, Williams RJ. Osmotic factors of dehardening in Cornus florida L. Plant Physiol. 1976;58:243–247. [PubMed]
  • Wolfe J, Dowgert MF, Steponkus PL. Mechanical study of the deformation and rupture of the plasma membranes of protoplasts during osmotic expansions. J Membr Biol. 1986;93:63–74.
  • Zelenina M, Brismar H. Osmotic water permeability measurements using confocal laser scanning microscopy. Eur Biophys J. 2000;29:165–171. [PubMed]
  • Zhang R, Logee KA, Verkman AS. Expression of mRNA coding for kidney and red cell water channels in Xenopus oocytes. J Biol Chem. 1990;265:15375–15378. [PubMed]
  • Zhang R, Verkman AS. Water and urea permeability properties of Xenopus oocytes: expression of mRNA from toad urinary bladder. Am J Physiol. 1991;260:C26–C34. [PubMed]
  • Zimmermann U. Physics of turgor- and osmoregulation. Annu Rev Plant Physiol. 1978;29:121–148.