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Biochem Res Int. 2011; 2011: 850924.
Published online 2010 November 30. doi:  10.1155/2011/850924
PMCID: PMC2997495

The Role of System-Specific Molecular Chaperones in the Maturation of Molybdoenzymes in Bacteria

Abstract

Biogenesis of prokaryotic molybdoenzymes is a complex process with the final step representing the insertion of a matured molybdenum cofactor (Moco) into a folded apoenzyme. Usually, specific chaperones of the XdhC family are required for the maturation of molybdoenzymes of the xanthine oxidase family in bacteria. Enzymes of the xanthine oxidase family are characterized to contain an equatorial sulfur ligand at the molybdenum center of Moco. This sulfur ligand is inserted into Moco while bound to the XdhC-like protein and before its insertion into the target enzyme. In addition, enzymes of the xanthine oxidase family bind either the molybdopterin (Mo-MPT) form of Moco or the modified molybdopterin cytosine dinucleotide cofactor (MCD). In both cases, only the matured cofactor is inserted by a proofreading process of XdhC. The roles of these specific XdhC-like chaperones during the biogenesis of enzymes of the xanthine oxidase family in bacteria are described.

1. Introduction

Molybdenum is a transition metal that is incorporated as a biologically active cofactor (molybdenum cofactor, Moco) in a class of widely distributed proteins collectively known as molybdoenzymes [1]. Moco is associated with a wide range of redox enzymes and is found in most organisms from bacteria to humans. The metal in Moco is coordinated to a pterin derivative called molybdopterin to form the molybdenum-containing molybdopterin (Mo-MPT) cofactor [2]. A wide variety of transformations are catalyzed by these enzymes at carbon, sulfur, and nitrogen atoms, which include the transfer of an oxygen group or two electrons to or from the substrate. The mononuclear molybdenum enzymes are categorized on the basis of the structures of their molybdenum centers, dividing them into three families, each with a distinct active site structure and a distinct type of reaction catalyzed: the xanthine oxidase family, the sulfite oxidase family, and the DMSO reductase family [1] (Figure 1). The xanthine oxidase family is characterized by an LMoVIOS(OH) core in the oxidized state, with one equivalent of the pterin cofactor (designated L) coordinated to the metal. These enzymes (including xanthine dehydrogenase [XDH] and xanthine oxidase [XO]) typically catalyze the hydroxylation of carbon centers [1]. Enzymes of the sulfite oxidase family coordinate a single equivalent of the pterin cofactor with an LMoVIO2(S-Cys) core in its oxidized state (the cysteine ligand is provided by the polypeptide) [1]. Members of this family (including sulfite oxidase and plant nitrate reductase) catalyze the transfer of an oxygen atom either to or from the substrate. The DMSO reductase family is diverse in both structure and function, but all members have two equivalents of the pterin cofactor bound to the metal. The molybdenum coordination sphere is usually completed by a single M=O group with a sixth ligand in the L2MVIO(X) core [1]. The sixth ligand, X, can be a serine, a cysteine, a selenocysteine, or a hydroxide and/or water molecule. The reactions catalyzed by members of this family frequently involve oxygen-atom transfer, but dehydrogenation reactions also occur. While in bacteria, molybdoenzymes of all three families are present, eukaryotes only harbour molybdoenzymes of the xanthine oxidase or sulfite oxidase family. In bacteria, the basic form of Mo-MPT is generally modified by the attachment of CMP or GMP to Mo-MPT, forming the molybdopterin cytosine dinucleotide cofactor (MCD) or the bis-molybdopterin guanine dinucleotide cofactor (bis-MGD) (Figure 1) [3]. While the bis-MGD form of Moco is exclusively distributed in enzymes of the DMSO reductase family, the MCD cofactor was identified in bacterial enzymes of the xanthine oxidase family. In this family, enzymes contain either Mo-MPT or MCD with an equatorial sulfur ligand coordinated to the molybdenum center [4].

Figure 1
The biosynthesis of Moco and the families of molybdoenzymes. Shown is a scheme of the biosynthetic pathway of Moco from MPT in bacteria. The proteins involved in the reactions are colored red. In bacteria, Mo-MPT can be further modified by the MobA function ...

Enzymes of the xanthine oxidase family are the best characterized mononuclear molybdenum-containing enzymes. With a few exceptions, they catalyze the hydroxylation of different types of substrates according to the following reaction:

RH+H2O=ROH+2H++2e.
(1)

This reaction occurs at the molybdenum center, which is, after interaction with substrate, reduced from Mo(VI) to Mo(IV). The two reducing equivalents generated in the course of the reaction are then transferred to an external electron acceptor by means of an electron transfer process mediated by other redox cofactors present in the structure of the protein. The crystal structure of the eight members of this family has been reported which are Desulfovibrio gigas aldehyde oxidoreductase (DgAOR) [5], Desulfovibrio desulfuricans aldehyde oxidoreductase (DdAOR) [6], Bos taurus xanthine oxidoreductase (bXOR) [7], Rhodobacter capsulatus xanthine dehydrogenase (RcXDH, Figure 2) [8], Pseudomonas putida Quinoline 2-oxidoreductase (PpQOR) [9], Thauera aromatica 4-hydroxylbenzoyl-CoA reductase (Ta 4-HBCR) [10] and the carbon monoxide dehydrogenase (CODH) from Oligotropha carboxidovorans [11] and Hydrogenophaga pseudoflava [12]. While bXOR and RcXDH contain Mo-MPT, all other crystallized bacterial enzymes were identified to bind MCD [13]. The majority of these enzymes (with the exception of 4-Hydroxybenzoyl-CoA reductase) are complex metalloflavoproteins that contain two nonidentical [2Fe2S] clusters, FAD, and the Moco as catalytically acting units (Figure 2) [14]. By contrast, CODH, which catalyzes a different reaction to that shown in (1), the conversion of CO to CO2 without cleavage of the CH bond, shows an active site that has never been observed in molybdoenzymes containing the pterin cofactor [15]. This comprises a dinuclear heterometal [CuSMoO2] cluster in which Mo and Cu ions are bridged by a sulfur ligand. CO oxidation is a source of energy for a wide diversity of prokaryotes and is an important process within the global carbon cycle. The enzyme CODH catalyzes the oxidation of CO and water to produce carbon dioxide, two electrons, and two protons [15]. The electrons are transferred to an electron transfer chain and used to generate a proton gradient across the membrane.

Figure 2
Overall structure and cofactor arrangement of R. capsulatus XDH. R. capsulatus XDH forms an (αβ)2 heterotetramer. The XdhA subunits are drawn in light green and light blue and the XdhB subunits in dark green and dark blue. The [2Fe-2S] ...

Xanthine oxidoreductase (XOR; which can be splitted into XDH, EC 1.17.1.4, and XO, EC 1.17.3.2) and aldehyde oxidase (AO; EC 1.2.3.1) are the best characterized members of the xanthine oxidase family [14, 16]. XOR has been the object of many reports and has long been recognized as the key enzyme in the catabolism of purines (Figure 2), oxidizing hypoxanthine to xanthine and xanthine to the terminal catabolite, uric acid, with the concomitant reduction of NAD+ (XDH) or O2 (XO) [17, 18]. XORs are of considerable medical interest, since this enzyme system in humans is implicated in gout and hyperuricemia, and its activity is responsible for postischaemic reperfusion injury. In bacteria, uric acid in most cases is further degraded to urea which is hydrolyzed to NH4 + and CO2 and thus can serve as a nitrogen and/or carbon source [19]. It was shown that R. capsulatus has the ability to utilize purines as sole nitrogen source [19]. While the biochemical function of XOR is well established, the biochemical and physiological function of AO is still largely obscure. The overall level of similarity between AO and XOR proteins is approximately 50%, which clearly indicates that the two proteins originated from a common ancestral precursor [14]. In humans, single monogenic deficits for any AO have not been described yet. AO is characterized by broad substrate specificity, and this makes it an important enzyme in the metabolism of drugs and xenobiotics. The physiological role of DgAOR was proposed to be linked to the degradation of polyglucose [5]. Also, in contrast to the other molybdo-flavoenzymes, DgAOR lacks the FAD domain [5]. Bacterial AOR can have a preference for aromatic aldehydes, as shown for the aldehyde oxidoreductase PaoABC from E. coli [20]. The physiological role of PaoABC in E. coli has been suggested to catalyze the detoxification of aromatic aldehydes to their corresponding less toxic acids in the periplasm [20].

The crystal structures of several molybdoenzymes revealed that Moco is deeply buried inside the proteins, at the end of a funnel-shaped passage giving access only to the substrate [21] (Figure 2). This implied the requirement of specific chaperones for each molybdoenzyme, to facilitate the insertion of Moco. For R. capsulatus XDH, the XdhC protein has not only been identified to be involved in Moco binding and formation of the terminal sulfur ligand, but was also shown to be involved in the final folding and maturation of apo-XDH after Moco insertion [22]. In prokaryotes, a number of specific chaperones were identified, also for members of the DMSO reductase family, like TorD for E. coli and trimethylamine oxide (TMAO) reductase (TorA) or NarJ for E. coli nitrate reductase A (NarGHI) [2326]. This paper will focus on the molecular chaperones involved in the maturation of enzymes from the xanthine oxidase family in bacteria. At the end, a short comparison to the eukaryotic system is presented.

2. In Bacterial Genomes, Structural Genes Encoding for Members of the Xanthine Oxidase Family and Molecular Chaperones Are Clustered

For R. capsulatus XDH, two genes, xdhA and xdhB, encode the polypeptides of the active enzyme [19]. XdhA was shown to bind the FAD cofactor and two [2Fe2S] clusters, and XdhB was shown to bind Mo-MPT (Figure 2) [8]. Immediately downstream of xdhB, a third gene was identified, designated xdhC, which is cotranscribed with xdhAB (Figure 3). Interposon mutagenesis revealed that the xdhC gene product is required for XDH activity [22]. However, XdhC is not a subunit of active XDH, which forms an (αβ)2 heterodimer in R. capsulatus (Figure 2). It was shown that XdhC neither is a transcriptional regulator for xdh gene expression nor influences XDH stability. The absence of Mo-MPT from XDH isolated from an R. capsulatus xdhC mutant strain indicated that XdhC might be a specific chaperone facilitating the insertion of Mo-MPT into XDH [22].

Figure 3
Schematic overview and organisation of operons including xdhC-like genes. Shown is the operon organization of different operons encoding for molybdoenzymes of the xanthine oxidase family of different bacteria. Operons of characterized molybdoenzymes are ...

Genes similar to R. capsulatus xdhC have also been identified in a number of other prokaryotes. In some cases, a similar operon organization compared to the one present for R. capsulatus XDH was identified. However, so far, R. capsulatus XdhC is the only member of this family which has been characterized both on the genetic level and on the protein level [28].

Pseudomonas aeruginosa contains an xdh operon consisting of the genes xdhABC, like the one identified in R. capsulatus (Figure 3). Here, also xdhAB code for the structural genes of XDH with a similar subunit composition as identified for R. capsulatus XDH. An essential role of P. aeruginosa XdhC for XDH has not been demonstrated yet, for example, by interposon mutagenesis or on the purified proteins. However, for P. aeruginosa XdhC, it was shown that coexpression with the xdhAB structural genes from Comamonas acidovorans in a heterologous system in E. coli resulted in the production of an active XDH [29]. In the absence of P. aeruginosa XdhC, a lower Mo-MPT content was identified in the purified protein [29]. This suggested a similar role for P. aeruginosa XdhC in Mo-MPT insertion and XDH maturation as shown for R. capsulatus XdhC.

In E. coli, the gene cluster for a periplasmic aldehyde oxidoreductase consists of paoABCD (Figure 3). The paoABC genes code for the three subunits of the trimeric aldehyde oxidoreductase, with PaoA binding two distinct [2Fe2S] clusters, PaoB binding FAD and PaoC containing MCD [20]. PaoD is predicted to be involved in MCD binding, maturation, and insertion into PaoC, since expression of paoABC in the absence of the paoD gene leads to inactive and instable PaoABC protein devoid of MCD [20]. Two additional open reading frames have been identified in E. coli, coding for XDH homologues, xdhABC and xdhD [19, 30, 31]. While both are predicted to have XDH activities, an XdhC homologue is not cotranscribed with these genes (Figure 3). However, a second XdhC homologue has been identified in E. coli, designated YqeB, which is organized in a single transcriptional unit in the genome. It remains to be elucidated whether YqeB is the XdhC-like chaperone shared by E. coli XdhD and XdhABC.

Three genes, coxL, coxM, and coxS (for large, medium, and small subunits) encode the polypeptides for the CODH enzyme in O. carboxidovorans OM5 (Figure 3) [27]. Two heterotrimers, each composed of one CoxL, CoxM, and CoxS subunit, combine to form a functional aerobic CODH enzyme. The large subunit contains Moco, the medium subunit binds FAD, and the small subunit has two [2Fe2S] clusters [11]. In addition to these three genes, a number of other accessory genes have also been identified (CoxB, CoxC, CoxH, CoxD, CoxE, CoxF, CoxG, CoxI, and CoxK) that are believed to be required in the processes of regulation, posttranslational modification, and anchorage of the CODH complex to the cytoplasmic membrane. A number of these accessory genes are membrane-bound proteins themselves (CoxB, CoxC, CoxH, and CoxK), containing several transmembrane helices, and indeed, in O. carboxidovorans OM5, the CODH enzyme itself has been observed to be associated with the inner cytoplasmic membrane [27]. By sequence analysis, several promoters were annotated within the gene region containing 12 genes essential for carboxidotrophic utilization (Figure 3) [27]. CoxD was shown to be a MoxR-like AAA+ ATPase with a predicted function in the stepwise introduction of sulfur and copper in the [CuSMoO2] center of the enzyme [32]. The role of CoxF and CoxI was not characterized yet, but both proteins share amino acid homologies to R. capsulatus XdhC (Figure 4).

Figure 4
Amino acid sequence alignment of XdhC-like proteins from different bacteria. Shown is an amino acid sequence alignment of the XdhC-like proteins organized in operon structures (from Figure 1) from R. capsulatus (RcXdhC), E. coli (EcPaoD), P. aeruginosa ...

In contrast, the organization of the B. subtilis puc operon differs as the gene for the XdhC homologue, pucA, is located upstream of the structural genes pucCDE encoding for an XDH-like protein (Figure 3) [33]. Downstream of pucA, the pucB gene is located which encodes for a homologue to the CTP: molybdopterin cytidylyltransferase MocA involved in MCD biosynthesis (addition of the CMP moiety to Mo-MPT, [34]) (Figure 1). A knockout of pucA and pucB in B. subtilis decreased growth on hypoxanthine or guanosine as nitrogen source without affecting growth on ammonia or uric acid [33]. PucA has not been purified or characterized on the molecular level so far.

In Arthrobacter nitroguajacolicus, a linear catabolic plasmid containing a total of 103 open reading frames was shown to be responsible for quinaldine degradation [35]. The plasmid contains the operon qoxMLS encoding for the MCD-containing quinaldine dehydrogenase (QoxM-binding MCD, QoxL-binding FAD, and QoxS-binding 2 [2Fe2S] cluster). An XdhC homologue is located immediately downstream of QoxMLS [35]. The qoxMLS genes for quinaldine dehydrogenase were cloned into an expression vector and introduced into P. putida KT2440, resulting in the production of an active quinaldine dehydrogenase [35]. This showed that the P. putida XdhC homologue was functionally capable to insert the MCD cofactor into A. nitroguajacolicus QoxM. Neither the P. putida nor the A. nitroguajacolicus XdhC homologues were characterized on the molecular level so far.

Figure 4 shows an amino acid sequence alignment of the XdhC-like proteins encoded by these operon structures. From the amino acid sequence alignment, it becomes clear that the overall sequence identity of the XdhC-like proteins from different bacteria is not high (~15%–30%). There are only very few highly conserved amino acids which are present in all XdhC-like proteins. However, one cysteine residue is conserved through almost all XdhC-like proteins present in the database, which corresponds to cysteine 82 in R. capsulatus XdhC (highlighted in red in Figure 4).

3. A Phylogenetic Point of View of the XdhC Family

The XdhC family of proteins contains hundreds of members that are bacterial and archaeal proteins, but no homologous sequences are present in eukaryotic genomes. While several organisms such as R. capsulatus contain only a single XdhC homologue, a large number of organisms like Rhodobacter sphaeroides or E. coli contain two or more homologues to XdhC. XdhC homologues are also present on strain-specific megaplasmids required for metabolic pathways like the one identified for quinaldine degradation in A. nitroguajacolicus Rü61a [35]. As discussed above, xdhC genes can be present in operons structures in conjunction with their respective structural genes encoding a member of the xanthine oxidase family (Figure 5, marked in bold). In some organisms, a gene organization was identified where genes encoding for XdhC homologues are cotranscribed with genes essential for Moco biosynthesis (Figure 5, underlined).

Figure 5
Phylogenetic tree of XdhC homologues. Protein phylogeny of XdhC homologues based on a full length sequence alignment. The tree was constructed by the neighbor-joining method from a matrix of estimated numbers of amino acid substitutions per site calculated ...

As obvious from the phylogenetic tree, XdhC homologues being part of XOR operons in different Proteo- and Actinobacteria are clustered. These include XdhC of R. capsulatus XDH and P. aeruginosa XDH, which both contain the Mo-MPT form of Moco. In contrast, PucA, the chaperone of B. subtilis XDH [33], forms a cluster with distinct XdhC homologues in different Bacillus strains. Sequence alignments and phylogenetic analysis of the Moco-containing subunits indicate that these XDH homologues likely contain the MCD form of Moco. Thus, Mo-MPT and MCD-containing XDHs and their respective chaperones might have originated from a common ancestor but have evolved separately during evolution.

The operon organization of the Moco chaperone and the molybdoenzyme partner, for example, like in R. capsulatus xdhABC [19, 22] or E. coli paoABCD [20], might indicate that XdhC and PaoD are the system-specific molecular chaperones for R. capsulatus XDH and E. coli AOR, respectively. Other XdhC homologues, including E. coli YqeB, which are not organized in an operon structure with a specific molybdoenzyme, might represent examples for Moco-inserting chaperones responsible for several molybdoenzyme partners. Examples include the flavobacterium Gramella forsetii where an XdhC homologue is a part of an operon containing two putative molybdoenzymes and several putative Moco biosynthesis proteins. A similar organization is present on the megaplasmid pA01 of Arthrobacter nicotinovorans which includes genes for nicotine dehydrogenase and the ketone dehydrogenase [36]. This megaplasmid contains a single xdhC gene which is cotranscribed with a mocA gene [37]. Since detailed analyses of the role of these XdhC homologues for several molybdoenzymes have not been analyzed in detail yet, a shared role of these chaperones for several molybdoenzymes remains speculative.

A more complex organization is present in O. carboxidovorans. The cox gene cluster of O. carboxidovorans megaplasmid pHCG3 encodes for two XdhC homologues, coxF and coxI (Figure 3) [27]. While the exact role of these homologues remains to be elucidated [38], phylogenetic analysis shows that CoxF is more similar to XdhC homologues found in other putative CODH operons and CoxI shows the highest similarity to E. coli PaoD. Moreover, the genome of O. carboxidovorans contains two additional genomic homologues of XdhC, one cotranscribed with the genes for a putative XDH and the other located in an operon with a MocA homologue.

4. The Absence of XdhC Affects the Maturation of the Molybdopartner

To date, a detailed characterization has only been performed for R. capsulatus XdhC [22, 28]. To analyze the function of XdhC for XDH in R. capsulatus, inactive XDH was purified from an R. capsulatus xdhC mutant strain [22, 28]. Analysis of the molybdenum cofactor content of this enzyme demonstrated that in the absence of XdhC, no Mo-MPT cofactor was present in the XdhAB heterotetramer. In contrast, absorption spectra of inactive XDH isolated from the xdhC mutant revealed the presence of iron-sulfur clusters and flavin adenine dinucleotide, demonstrating that XdhC is not required for the insertion of these cofactors. In the absence of Mo-MPT, XdhC remained associated with the inactive XDH heterodimer devoid of Mo-MPT [22, 28]. In addition, a heterologous system for the expression of R. capsulatus XDH in E. coli was established [39]. Here, the R. capsulatus xdhABC genes were coexpressed in E. coli, which resulted in a production of a 100% active XDH containing a full complement of the equatorial Mo=S ligand [40]. In the absence of XdhC, Mo-MPT was inserted into XDH in E. coli; however, the content of the equatorial sulfur ligand of molybdenum was drastically reduced [28]. This implied that XdhC is involved in the maturation of Mo-MPT by the addition of the equatorial sulfur ligand to the cofactor prior to the insertion into the XDH apoenzyme (in case of R. capsulatus).

The E. coli strain used in this study contained a deletion of the mobAB genes involved in bis-MGD formation [41], which resulted in an accumulation of Mo-MPT to unphysiological high concentrations. Thus, it is believed that Mo-MPT is unspecifically inserted into R. capsulatus XDH in E. coli during expression likely due to the accumulation of Mo-MPT. Whether one of the E. coli XdhC-like proteins is capable of inserting Mo-MPT into R. capsulatus XDH has not been investigated so far. In E. coli, the level of Moco sulfuration drastically depended on the oxygen concentration, since the amount of sulfurated Moco in XDH largely decreased with a high aeration of the expression cultures [28]. This implied a protective role of XdhC during Moco sulfuration. Similar results were also reported for C. acidovorans XDH expressed in E. coli [29].

For the characterization of XdhC, the protein was purified after heterologous expression in E. coli [28]. Characterization of the purified protein showed that XdhC is a dimer in solution and the purified protein is able to bind stoichiometric amounts of Mo-MPT or MPT, with dissociation constants of 3.6 ± 0.1 μM and 3.5 ± 0.3 μM, respectively [28]. It was also shown that XdhC-bound Mo-MPT can be inserted into Moco-free apo-XDH. Analysis of protein-protein interactions showed that XdhC specifically interacts with the XdhB subunit of XDH for Mo-MPT insertion [28]. The tight interaction of XdhC with XdhB underlined the specific role of XdhC in XDH maturation. For analysis of the role of the highly conserved cysteine residue Cys82 in R. capsulatus XdhC, site-directed mutagenesis was performed to exchange Cys82 by alanine. Unfortunately, all attempts to purify the XdhC-C82A variant failed, since this protein variant was highly unstable and precipitated completely during purification (unpublished results).

5. Formation of Sulfurated Moco

The investigations showed that XdhC binds Mo-MPT, which is produced by MoeA/MogA in the cell [42, 43] and protects it from oxidation until the terminal sulfur ligand is inserted. So far, proteins with a specific Moco sulfurase activity were identified in a number of eukaryotes. The first report was given by Wahl et al. [44] describing a mutation in the Drosophila melanogaster maroon-like locus (ma-l) which impaired the activity of XDH and AO, while the activity of sulfite oxidase remained unaffected [45]. Eukaryotic Moco sulfurase are two-domain proteins with an N-terminal domain showing homologies to bacterial L-cysteine desulfurase (E.C. 2.8.1.7) of the NifS family and a C-terminal domain with Moco-binding properties [46, 47], however, without homologies to the bacterial XdhC family. In general, L-cysteine desulfurase are homodimeric proteins that utilize pyridoxal 5-phosphate (PLP) to catalyze the reductive elimination of sulfur from L-cysteine, resulting in the formation of alanine and an enzyme-bound cysteine persulfide intermediate [48]. R. capsulatus and E. coli contain several L-cysteine desulfurase which could act as a sulfur donor for the sulfuration of Moco in these bacterial systems. The E. coli L-cysteine desulfurase are IscS, CsdA, and SufS [49], and the R. capsulatus homologues were named NifS (specific for nitrogenase), NifS2, NifS3, and NifS4 [50].

A specific role for the three R. capsulatus L-cysteine desulfurase NifS2, NifS3, and NifS4 had not been assigned before. Interposon mutagenesis of the nifS2, nifS3, and nifS4 genes showed that nifS2 strains displayed no particular XDH phenotype and that nifS3 and nifS4 are apparently essential for the viability of R. capsulatus [50]. To identify the specific L-cysteine desulfurase for the sulfuration of Moco in R. capsulatus, a direct E. coli two-hybrid screen was applied and the detected interaction partners were verified by surface plasmon resonance (SPR) measurements using the purified proteins. The L-cysteine desulfurase NifS4 of R. capsulatus was identified to specifically interact with XdhC with a dissociation constant of 0.64  μM [50]. It was identified that NifS4 sulfurates Moco while it is bound to XdhC. An interaction of NifS4 with XDH was excluded. Thus, the XdhC-NifS4 pair can be considered as the prokaryotic counterpart to specific Moco sulfatases identified in eukaryotes. However, since interposon mutagenesis of nifS4 was not successful, the corresponding protein appears to have an additional role in another essential sulfur transfer pathway in the cell. NifS4 mobilizes sulfur from L-cysteine by formation of a protein-bound persulfide intermediate and transfers this sulfur further to XdhC-bound Moco. This reaction was shown to be more effective than the chemical sulfuration of Moco using sulfide as sulfur source. Further studies clearly showed that Moco is sulfurated before the insertion into XDH, while it is bound to XdhC.

6. A Model for the Insertion of Sulfurated Moco and Maturation of Molybdoenzymes

The assembly of XDH is a highly ordered process, which involves the synthesis of the XdhA and XdhB subunits, the dimerization of both subunits, the insertion of FeSI, FeSII, and FAD into the XdhA subunit, the dimerization of two (αβ) dimers via the XdhB subunit, and finally, the insertion of sulfurated Moco into XdhB, resulting in an active enzyme (Figure 6) [51]. The biosynthesis of Moco is additionally a complex process involving more than a dozen different proteins [52, 53], with the insertion of sulfurated Moco by the XdhC protein being the last step of XDH maturation [22].

Figure 6
Model for the role of XdhC in the assembly of R. capsulatus XDH. The assembly of R. capsulatus XDH involves the synthesis of the XdhA and XdhB subunits, the dimerization of both subunits, the insertion of FeSI, FeSII, and FAD into the XdhA subunit, dimerization ...

Moco is produced from MPT by the MogA-MoeA complex, catalyzing the ATP-dependent ligation of the molybdenum atom to MPT (Figure 6) [54, 55]. Synthesized Mo-MPT can subsequently be transferred either to MobA, converting it into bis-MGD (which is inserted into enzymes of the DMSO reductase family) [56], or to XdhC, forming the sulfurated form of Moco by exchange of an oxoligand with sulfur (which is inserted into XDH). XdhC interacts with MobA and thereby inhibits the transfer of Moco to MobA [43]. Further, it is believed that XdhC dissociates from this complex to interact with the L-cysteine desulfurase NifS4. NifS4 directly interacts with XdhC and exchanges the equatorial oxygen ligand specifically by a sulfur ligand. The sulfur for this reaction originated from L-cysteine and a persulfide group is formed on NifS4 in the course of the reaction. Further, XdhC has to dissociate from NifS4 after the sulfuration reaction to interact with XdhB. The present results for R. capsulatus XDH demonstrate that dimerization via the β-subunits is required to stabilize a structure of the protein that makes the protein suitable for Moco insertion [51]. Since XdhC is a dimer itself, it is suggested that the XdhC dimer interacts with the XdhB dimer simultaneously inserting matured and sulfurated Mo-MPT into both active sites of XdhB (Figure 6). The insertion of sulfurated Moco into XDH is strictly regulated in R. capsulatus, because in vivo dioxo-Moco is not inserted into XDH [28]. The mode of control of this step has not been identified to date. After this reaction, XDH is correctly folded and XdhC dissociates from the complex. Since XdhC is very labile in the absence of Moco, it is believed that after performing the insertion reaction, XdhC is degraded in the cell.

Thus, XdhC performs several control reactions: (i) to ensure that Moco is sulfurated by the interaction with the L-cysteine desulfurase NifS4 [50] before insertion into XDH and (ii) to insert the sulfurated Moco in the formed (αβ)2 heterotetramer of XDH. Because Moco is deeply buried in the protein, it is also believed that XdhC acts as a chaperone being involved in proper folding of XDH after Moco insertion [22]. The model shows that apo-XDH exists in a Moco competent “open” conformation until the insertion of sulfurated Moco and that after the insertion reaction, the protein adapts the final active “closed” conformation, which is incapable to accept Moco (Figure 6).

7. Functional XdhC Homologues in Eukaryotes

Eukaryotic Moco sulfurase are two-domain proteins containing one protein domain with homologies to L-cysteine desulfurase and a second protein domain belonging to a superfamily of β-strand-rich domains, named MOSC domains [57]. These MOSC domain proteins are characterized to contain an absolutely conserved cysteine residue. The MOSC domain was predicted to be a sulfur-carrier domain that receives the sulfur abstracted by the PLP-dependent L-cysteine desulfurase domain on its cysteine residue and delivers it for the formation of, for example, sulfurated Moco in case of the Moco sulfurase [57]. Bacterial homologues to the MOSC superfamily were also identified, like the E. coli YiiM protein [58], and one to four orthologs of YiiM per genome are present in diverse bacteria such as α- and γ-proteobacteria and gram-positive bacteria [57]. The E. coli YiiM protein was characterized to be involved in the detoxification of 6-N-hydroxylaminopurine to adenine, a pathway which depends on the presence of active Moco [58]. It could be clearly shown that the role of YiiM is distinct from the formation of sulfurated Moco for enzymes of the xanthine oxidase family [58]. However, the precise role and the involvement of Moco in the reaction remains to be elucidated for YiiM.

The currently best characterized eukaryotic Moco sulfurase is ABA3 from Arabidopsis thaliana [59, 60]. ABA3 is composed of an N-terminal NifS-like domain and a C-terminal MOSC domain. L-cysteine desulfurase activity and sulfur transferase activity of ABA3 were shown to be essential for the activity of AO, being involved in abscisic acid (ABA) biosynthesis in plants. It has been suggested that a persulfide sulfur is transferred from the NifS-like domain of ABA3 to the Moco in AO of plants [60]. More recent studies showed that the C-terminal MOSC domain is able to bind Moco in a 1 : 1 ratio with a dissociation constant of 0.55 ± 0.14 μM, implying that the C-terminus of ABA3 represents the functional homologue of XdhC in bacteria [61]. In addition, it was shown that the Moco bound on ABA3 existed in its sulfurated form. However, in contrast to the case in bacteria, Wollers et al. [61] proposed that the role of the ABA3 C-terminus in activation of XDH and AO occurs posttranslationally, after the insertion of Mo-MPT in these enzymes. Evidence for this assumption was obtained from experiments showing that the activity of these enzymes in ABA3 C-terminus mutant extracts (sir3-3) was increased by a chemical, nonenzymatical sulfuration procedure. This implied that AO in plants is present in both a sulfo and a desulfo form, which can posttranslationally be activated by ABA3. Thus, in plants, AO and XDH remain inactive until one of the two oxygen ligands is replaced by a sulfur atom delivered by ABA3. By this mechanism, it was suggested that the plant is able to rapidly increase the activities of AO and XDH without de novo synthesis of the apoproteins, representing a mechanism of rapid posttranslational regulation [59, 61]. Unfortunately, the authors did not consider the possibility that in the sir3-3 ABA3 mutant, the nonsulfurated Moco was inserted unspecifically into AO. Following the model by Bittner's group, it remains to be elucidated how AO and XDH receive their sulfurated Moco. In plant AO and XDH, this could occur either by an exchange of a sulfurated with a nonsulfurated Moco by aid of ABA3 or by exchange of solely the sulfide-ligand. Both models are largely different from the system in bacteria, where a quality control mechanism guarantees that only sulfurated Moco is inserted into enzymes of the xanthine oxidase family, and a posttranslational regulation of enzyme activity via its sulfuration level does not occur. In bacteria, after insertion of sulfurated Moco which is deeply buried into the enzyme, the enzyme is correctly folded. Thus, eukaryotic Moco sulfurase must have developed a system for the unfolding of the inactive holoenzyme to either extract the Moco from it or to sulfurate the Moco while bound to the target enzyme. This shows that for a similar function, the eukaryotic and the prokaryotic systems must have evolved independently.

8. Concluding Remarks

This paper highlights the key roles of the bacterial XdhC family of system-specific chaperones in Moco modification and insertion into enzymes of the xanthine oxidase family. Due to a lack of information on other XdhC-like proteins, this paper is mainly focussed on the role of R. capsulatus XdhC for XDH. However, on the basis of sequence similarities and gene organizations in other bacteria, we believe that R. capsulatus XdhC represents an example of a large family of XdhC-like proteins which perform the same function for their specific molybdoenzyme partners. These specific molecular XdhC-like chaperones are involved in the late steps of Moco biosynthesis by protecting Mo-MPT against oxidation for its final maturation step, the exchange of the equatorial oxygen ligand at the Mo center against a sulfide group. All XdhC-like proteins contain a highly conserved cysteine residue, which might perform a similar function as the conserved cysteine in the MOSC-like domains of eukaryotic Moco sulfatases. So far, the role of this cysteine residue is not completely clear in both systems. It could either act as a sulfur-transferring cysteine on which a persulfide is formed during the reaction, but it also could serve as a ligand to the protein-bound Mo-MPT, thus, either stabilizing the bound Moco or representing a ligand to generate an electron environment that facilitates the sulfur/oxygen exchange reaction. After synthesis of the mono-oxo Moco, the cofactor is specifically inserted into a target enzyme. Conclusively, the XdhC family of proteins must specifically recognize and interact with its target protein partner. During the maturation step of the apomolybdoenzyme, XdhC must stabilize a competent state of the apoenzyme for cofactor insertion. The matured Moco is inserted into the catalytic site of the enzyme which, in its final form, is deeply buried in the enzyme. After final folding of the molybdoenzyme, XdhC has performed its role and is no longer required for enzyme activity and consequently dissociates from the holomolybdoenzyme. In total, these XdhC proteins perform versatile roles for Moco and molybdoenzyme maturation, involving the interaction with different proteins, which has to be highly specific. Attempts to solve the crystal structure of XdhC are under investigation, which will shed light into the interaction sites and Moco binding site of this protein. In eukaryotes, functional homologues to XdhC exist, which have further evolved from this system and are fused to one interaction partner, the L-cysteine desulfurase which forms sulfurated Mo-MPT. Further investigations will clarify whether the C-terminal MOSC domain of eukaryotic Moco sulfurase performs a similar role like XdhC in bacteria. In total, the XdhC family represents an exquisite model to study the maturation of molybdoenzymes.

Acknowledgments

This work was supported by continuous Deutsche Forschungsgemeinschaft grants, by the DAAD PROCOPE Program, and the Cluster of Excellence “Unifying Concepts in Catalysis” coordinated by the Technische Universität Berlin. Authors thank K. V. Rajagopalan (Duke University) for his constant support.

References

1. Hille R. The mononuclear molybdenum enzymes. Chemical Reviews. 1996;96(7):2757–2816. [PubMed]
2. Rajagopalan KV, Johnson JL. The pterin molybdenum cofactors. Journal of Biological Chemistry. 1992;267(15):10199–10202. [PubMed]
3. Schwarz G. Molybdenum cofactor biosynthesis and deficiency. Cellular and Molecular Life Sciences. 2005;62(23):2792–2810. [PubMed]
4. Hille R. Molybdenum-containing hydroxylases. Archives of Biochemistry and Biophysics. 2005;433(1):107–116. [PubMed]
5. Romão MJ, Archer M, Moura I, et al. Crystal structure of the xanthine oxidase-related aldehyde oxido-reductase from D. gigas . Science. 1995;270(5239):1170–1176. [PubMed]
6. Rebelo J, Macieira S, Dias JM, et al. Gene sequence and crystal structure of the aldehyde oxidoreductase from Desulfovibrio desulfuricans ATCC 27774. Journal of Molecular Biology. 2000;297(1):135–146. [PubMed]
7. Enroth C, Eger BT, Okamoto K, Nishino T, Nishino T, Pai EF. Crystal structures of bovinemilk xanthine dehydrogenase and xanthine oxidase: structure-based mechanism of conversion. Proceedings of the National Academy of Sciences of the United States of America. 2000;97(20):10723–10728. [PubMed]
8. Truglio JJ, Theis K, Leimkühler S, Rappa R, Rajagopalan KV, Kisker C. Crystal structures of the active and alloxanthine-inhibited forms of xanthine dehydrogenase from Rhodobacter capsulatus . Structure. 2002;10(1):115–125. [PubMed]
9. Bonin I, Martins BM, Purvanov V, Fetzner S, Huber R, Dobbek H. Active site geometry and substrate recognition of the molybdenum hydroxylase quinoline 2-oxidoreductase. Structure. 2004;12(8):1425–1435. [PubMed]
10. Unciuleac M, Warkentin E, Page CC, Boll M, Ermler U. Structure of a xanthine oxidase-related 4-hydroxybenzoyl-CoA reductase with an additional [4Fe-4S] cluster and an inverted electron flow. Structure. 2004;12(12):2249–2256. [PubMed]
11. Dobbek H, Gremer L, Meyer O, Huber R. Crystal structure and mechanism of CO dehydrogenase, a molybdo iron-sulfur flavoprotein containing S-selanylcysteine. Proceedings of the National Academy of Sciences of the United States of America. 1999;96(16):8884–8889. [PubMed]
12. Hänzelmann P, Dobbek H, Gremer L, Huber R, Meyer O. The effect of intracellular molybdenum in Hydrogenophaga pseudoflava on the crystallographic structure of the seleno-molybdo-iron-sulfur flavoenzyme carbon monoxide dehydrogenase. Journal of Molecular Biology. 2000;301(5):1221–1235. [PubMed]
13. Brondino CD, Romão MJ, Moura I, Moura JJG. Molybdenum and tungsten enzymes: the xanthine oxidase family. Current Opinion in Chemical Biology. 2006;10(2):109–114. [PubMed]
14. Garattini E, Mendel R, Romão MJ, Wright R, Terao M. Mammalian molybdo-flavoenzymes, an expanding family of proteins: structure, genetics, regulation, function and pathophysiology. Biochemical Journal. 2003;372(1):15–32. [PubMed]
15. Dobbek H, Gremer L, Kiefersauer R, Huber R, Meyer O. Catalysis at a dinuclear [CuSMo(=O)OH] cluster in a CO dehydrogenase resolved at 1.1-Å resolution. Proceedings of the National Academy of Sciences of the United States of America. 2002;99(25):15971–15976. [PubMed]
16. Garattini E, Fratelli M, Terao M. Mammalian aldehyde oxidases: genetics, evolution and biochemistry. Cellular and Molecular Life Sciences. 2008;65(7-8):1019–1048. [PubMed]
17. Nishino T. The conversion of xanthine dehydrogenase to xanthine oxidase and the role of the enzyme in reperfusion injury. Journal of Biochemistry. 1994;116(1):1–6. [PubMed]
18. Nishino T, Okamoto K, Kawaguchi Y, et al. Mechanism of the conversion of xanthine dehydrogenase to xanthine oxidase: identification of the two cysteine disulfide bonds and crystal structure of a non-convertible rat liver xanthine dehydrogenase mutant. Journal of Biological Chemistry. 2005;280(26):24888–24894. [PubMed]
19. Leimkühler S, Kern M, Solomon PS, et al. Xanthine dehydrogenase from the phototrophic purple bacterium Rhodobacter capsulatus is more similar to its eukaryotic counterparts than to prokaryotic molybdenum enzymes. Molecular Microbiology. 1998;27(4):853–869. [PubMed]
20. Neumann M, Mittelstädt G, Iobbi-Nivol C, et al. A periplasmic aldehyde oxidoreductase represents the first molybdopterin cytosine dinucleotide cofactor containing molybdo-flavoenzyme from Escherichia coli . FEBS Journal. 2009;276(10):2762–2774. [PubMed]
21. Kisker C, Schindelin H, Rees DC. Molybdenum-cofactor-containing enzymes: structure and mechanism. Annual Review of Biochemistry. 1997;66:233–267. [PubMed]
22. Leimkühler S, Klipp W. Role of XDHC in molybdenum cofactor insertion into xanthine dehydrogenase of Rhodobacter capsulatus . Journal of Bacteriology. 1999;181(9):2745–2751. [PMC free article] [PubMed]
23. Blasco F, Dos Santos J-P, Magalon A, et al. NarJ is a specific chaperone required for molybdenum cofactor assembly in nitrate reductase A of Escherichia coli . Molecular Microbiology. 1998;28(3):435–447. [PubMed]
24. Ilbert M, Méjean V, Giudici-Orticoni M-T, Samama J-P, Iobbi-Nivol C. Involvement of a mate chaperone (TorD) in the maturation pathway of molybdoenzyme TorA. Journal of Biological Chemistry. 2003;278(31):28787–28792. [PubMed]
25. Tranier S, Iobbi-Nivol C, Birck C, et al. A novel protein fold and extreme domain swapping in the dimeric TorD chaperone from Shewanella massilia . Structure. 2003;11(2):165–174. [PubMed]
26. Genest O, Méjean V, Iobbi-Nivol C. Multiple roles of TorD-like chaperones in the biogenesis of molybdoenzymes. FEMS Microbiology Letters. 2009;297(1):1–9. [PubMed]
27. Santiago B, Schübel U, Egelseer C, Meyer O. Sequence analysis, characterization and CO-specific transcription of the cox gene cluster on the megaplasmid pHCG3 of Oligotropha carboxidovorans . Gene. 1999;236(1):115–124. [PubMed]
28. Neumann M, Schulte M, Jünemann N, Stöcklein W, Leimkühler S. Rhodobacter capsulatus XdhC is involved in molybdenum cofactor binding and insertion into xanthine dehydrogenase. Journal of Biological Chemistry. 2006;281(23):15701–15708. [PubMed]
29. Ivanov NV, Hubálek F, Trani M, Edmondson DE. Factors involved in the assembly of a functional molybdopyranopterin center in recombinant Comamonas acidovorans xanthine dehydrogenase. European Journal of Biochemistry. 2003;270(23):4744–4754. [PubMed]
30. Kozmin SG, Schaaper RM. Molybdenum cofactor-dependent resistance to N-hydroxylated base analogs in Escherichia coli is independent of MobA function. Mutation Research. 2007;619(1-2):9–15. [PMC free article] [PubMed]
31. Xi H, Schneider BL, Reitzer L. Purine catabolism in Escherichia coli and function of xanthine dehydrogenase in purine salvage. Journal of Bacteriology. 2000;182(19):5332–5341. [PMC free article] [PubMed]
32. Pelzmann A, Ferner M, Gnida M, Meyer-Klaucke W, Meyer O. The CoxD protein of Oligotropha carboxidovorans is a predicted AAA+ ATPase chaperone involved in the biogenesis of the CO dehydrogenase [CuSMoO2] cluster. Journal of Biological Chemistry. 2009;284(14):9578–9586. [PMC free article] [PubMed]
33. Schultz AC, Nygaard P, Saxild HH. Functional analysis of 14 genes that constitute the purine catabolic pathway in Bacillus subtilis and evidence for a novel regulon controlled by the PucR transcription activator. Journal of Bacteriology. 2001;183(11):3293–3302. [PMC free article] [PubMed]
34. Neumann M, Mittelstädt G, Seduk F, Iobbi-Nivol C, Leimkühler S. MocA is a specific cytidylyltransferase involved in molybdopterin cytosine dinucleotide biosynthesis in Escherichia coli . Journal of Biological Chemistry. 2009;284(33):21891–21898. [PMC free article] [PubMed]
35. Parschat K, Hauer B, Kappl R, Kraft R, Hüttermann J, Fetzner S. Gene cluster of Arthrobacter ilicis Ru61a involved in the degradation of quinaldine to anthranilate. Characterization and functional expression of the quinaldine 4-oxidase qoxLMS genes. Journal of Biological Chemistry. 2003;278(30):27483–27494. [PubMed]
36. Menéndez C, Igloi G, Henninger H, Brandsch R. A pAO1-encoded molybdopterin cofactor gene (moaA) of Arthrobacter nicotinovorans: characterization and site-directed mutagenesis of the encoded protein. Archives of Microbiology. 1995;164(2):142–151. [PubMed]
37. Baitsch D, Sandu C, Brandsch R, Igloi GL. Gene cluster on pAO1 of Arthrobacter nicotinovorans involved in degradation of the plant alkaloid nicotine: cloning, purification, and characterization of 2,6-dihydroxypyridine 3-hydroxylase. Journal of Bacteriology. 2001;183(18):5262–5267. [PMC free article] [PubMed]
38. Fuhrmann S, Ferner M, Jeffke T, Henne A, Gottschalk G, Meyer O. Complete nucleotide sequence of the circular megaplasmid pHCG3 of Oligotropha carboxidovorans: function in the chemolithoautotrophic utilization of CO, H2 and CO2 . Gene. 2003;322(1-2):67–75. [PubMed]
39. Leimkühler S, Hodson R, George GN, Rajagopalan KV. Recombinant Rhodobacter capsulatus xanthine dehydrogenase, a useful model system for the characterization of protein variants leading to xanthinuria I in humans. Journal of Biological Chemistry. 2003;278(23):20802–20811. [PubMed]
40. Leimkühler S, Stockert AL, Igarashi K, Nishino T, Hille R. The role of active site glutamate residues in catalysis of Rhodobacter capsulatus xanthine dehydrogenase. Journal of Biological Chemistry. 2004;279(39):40437–40444. [PubMed]
41. Palmer T, Vasishta A, Whitty PW, Boxer DH. Isolation of protein FA, a product of the mob locus required for molybdenum cofactor biosynthesis in Escherichia coli . European Journal of Biochemistry. 1994;222(2):687–692. [PubMed]
42. Leimkühler S, Angermüller S, Schwarz G, Mendel RR, Klipp W. Activity of the molybdopterin-containing xanthine dehydrogenase of Rhodobacter capsulatus can be restored by high molybdenum concentrations in a moeA mutant defective in molybdenum cofactor biosynthesis. Journal of Bacteriology. 1999;181(19):5930–5939. [PMC free article] [PubMed]
43. Neumann M, Stöcklein W, Leimkühler S. Transfer of the molybdenum cofactor synthesized by Rhodobacter capsulatus MoeA to XdhC and MobA. Journal of Biological Chemistry. 2007;282(39):28493–28500. [PubMed]
44. Wahl RC, Warner CK, Finnerty V, Rajagopalan KV. Drosophila melanogaster ma-l mutants are defective in the sulfuration of desulfo Mo hydroxylases. Journal of Biological Chemistry. 1982;257(7):3958–3962. [PubMed]
45. Finnerty V, McCarron M, Johnson GB. Gene expression in Drosophila: post-translational modification of aldehyde oxidase and xanthine dehydrogenase. Molecular and General Genetics. 1979;172(1):37–43. [PubMed]
46. Amrani L, Primus J, Glatigny A, Arcangeli L, Scazzocchio C, Finnerty V. Comparison of the sequences of the Aspergillus nidulans hxB and Drosophila melanogaster ma-I genes with nifS from Azotobacter vinelandii suggests a mechanism for the insertion of the terminal sulphur atom in the molybdopterin cofactor. Molecular Microbiology. 2000;38(1):114–125. [PubMed]
47. Mendel RR, Bittner F. Cell biology of molybdenum. Biochimica et Biophysica Acta. 2006;1763(7):621–635. [PubMed]
48. Zheng L, White RH, Cash VL, Dean DR. Mechanism for the desulfurization of L-cysteine catalyzed by the NIFs gene product. Biochemistry. 1994;33(15):4714–4720. [PubMed]
49. Kurihara T, Mihara H, Kato S-I, Yoshimura T, Esaki N. Assembly of iron-sulfur clusters mediated by cysteine desulfurases, IscS, CsdB and CSD, from Escherichia coli . Biochimica et Biophysica Acta. 2003;1647(1-2):303–309. [PubMed]
50. Neumann M, Stöcklein W, Walburger A, Magalon A, Leimkühler S. Identification of a Rhodobacter capsulatus L-cysteine desulfurase that sulfurates the molybdenum cofactor when bound to XdhC and before its insertion into xanthine dehydrogenase. Biochemistry. 2007;46(33):9586–9595. [PubMed]
51. Schumann S, Saggu M, Möller N, et al. The mechanism of assembly and cofactor insertion into Rhodobacter capsulatus xanthine dehydrogenase. Journal of Biological Chemistry. 2008;283(24):16602–16611. [PubMed]
52. Rajagopalan KV. Biosynthesis of the molybdenum cofactor in Escherichia coli and Salmonella . In: Neidhardt FC, editor. Cellular and Molecular Biology. Washington, DC, USA: ASM Press; 1996. pp. 674–679.
53. Schwarz G, Mendel RR, Ribbe MW. Molybdenum cofactors, enzymes and pathways. Nature. 2009;460(7257):839–847. [PubMed]
54. Nichols J, Rajagopalan KV. Escherichia coli MoeA and MogA: function in metal incorporation step of molybdenum cofactor biosynthesis. Journal of Biological Chemistry. 2002;277(28):24995–25000. [PubMed]
55. Nichols JD, Rajagopalan KV. In vitro molybdenum ligation to molybdopterin using purified components. Journal of Biological Chemistry. 2005;280(9):7817–7822. [PubMed]
56. Palmer T, Santini C-L, Iobbi-Nivol C, Eaves DJ, Boxer DH, Giordano G. Involvement of the narJ and mob gene products in distinct steps in the biosynthesis of the molybdoenzyme nitrate reductase in Escherichia coli . Molecular Microbiology. 1996;20(4):875–884. [PubMed]
57. Anantharaman V, Aravind L. MOSC domains: ancient, predicted sulfur-carrier domains, present in diverse metal-sulfur cluster biosynthesis proteins including Molybdenum cofactor sulfurases. FEMS Microbiology Letters. 2002;207(1):55–61. [PubMed]
58. Kozmin SG, Leroy P, Pavlov YI, Schaaper RM. YcbX and yiiM, two novel determinants for resistance of Escherichia coli to N-hydroxylated base analogues. Molecular Microbiology. 2008;68(1):51–65. [PMC free article] [PubMed]
59. Bittner F, Oreb M, Mendel RR. ABA3 is a molybdenum cofactor sulfurase required for activation of aldehyde oxidase and xanthine dehydrogenase in Arabidopsis thaliana . Journal of Biological Chemistry. 2001;276(44):40381–40384. [PubMed]
60. Heidenreich T, Wollers S, Mendel RR, Bittner F. Characterization of the NifS-like domain of ABA3 from Arabidopsis thaliana provides insight into the mechanism of molybdenum cofactor sulfuration. Journal of Biological Chemistry. 2005;280(6):4213–4218. [PubMed]
61. Wollers S, Heidenreich T, Zarepour M, et al. Binding of sulfurated molybdenum cofactor to the C-terminal domain of ABA3 from Arabidopsis thaliana provides insight into the mechanism of molybdenum cofactor sulfuration. Journal of Biological Chemistry. 2008;283(15):9642–9650. [PubMed]

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