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Rhesus monkeys (Macaca mulatta) are widely-used as experimental animals in biomedical research and are closely related to other laboratory macaques, such as cynomolgus monkeys (Macaca fascicularis), and to humans, sharing a last common ancestor from about 25 million years ago. Although rhesus monkeys have been studied extensively under field and laboratory conditions, research has been limited by the lack of genetic resources. The present study generated placenta full-length cDNA libraries, characterized the resulting expressed sequence tags, and described their utility for comparative mapping with human RefSeq mRNA transcripts.
From rhesus monkey placenta full-length cDNA libraries, 2000 full-length cDNA sequences were determined and 1835 rhesus placenta cDNA sequences longer than 100 bp were collected. These sequences were annotated based on homology to human genes. Homology search against human RefSeq mRNAs revealed that our collection included the sequences of 1462 putative rhesus monkey genes. Moreover, we identified 207 genes containing exon alterations in the coding region and the untranslated region of rhesus monkey transcripts, despite the highly conserved structure of the coding regions. Approximately 10% (187) of all full-length cDNA sequences did not represent any public human RefSeq mRNAs. Intriguingly, two rhesus monkey specific exons derived from the transposable elements of AluYRa2 (SINE family) and MER11B (LTR family) were also identified.
The 1835 rhesus monkey placenta full-length cDNA sequences described here could expand genomic resources and information of rhesus monkeys. This increased genomic information will greatly contribute to the development of evolutionary biology and biomedical research.
The rhesus monkey (Macaca mulatta) is one of the species of Macaca, an Old World monkey. On the basis of DNA sequence comparison complemented by fossil evidence, the divergence of humans and Old World monkeys is estimated at about 25 million years ago . The relationship between humans and rhesus monkeys is even more important because biomedical research has come to depend on these primates as experimental animal models . Due to their genetic, physiologic, and metabolic similarity to humans, this species serves as an essential research tool in neuroscience, behavioral biology, reproductive physiology, neuroendocrinology, endocrinology, cardiovascular studies, pharmacology and many other areas [3-5].
The draft sequence of the rhesus monkey genome, which has an important evolutionary position, was published in 2007 . The final challenge comes in the understanding of basic rhesus molecular biology through interpretation of the rhesus monkey genome. Transcriptome data could broaden the application of genome sequences. One of the most useful approaches obtaining large-scale sequence information is through the construction and sequencing of cDNA libraries . These libraries represent a collection of genes that have been expressed as mRNA in a given cell or tissue, and are especially useful for obtaining sequence information on the coding regions of the genome . Previous effort to catalogue the rhesus monkey transcriptome were based on expressed sequence tags (ESTs) used for the identification of genes, prediction of genes, and assessment of gene expression . Also, a comparative analysis of mRNA sequences may provide clues to the genetic information that affects the different phenotypes . However, the usefulness of EST clones is limited; because many EST clones lack the complete sequences of mRNAs, they cannot be used to reveal the primary structures of entire genes and encoded proteins . ESTs are useful for making a catalog of expressed genes, but not for further study of gene function. Consequently, genome-scale collections of the full-length cDNAs of expressed genes become important for the analysis of the structure and function of genes . In contrast to the great number of human full-length cDNA sequences in public databases such as the RefSeq mRNAs of the National Center for Biotechnology Information, only a small number of rhesus monkey mRNA sequences and ESTs have been deposited in public databases.
In this study, full-length-enriched cDNA libraries were constructed from rhesus monkey placenta using the oligo-capping method . This method can efficiently identify apparent rhesus monkey homologs of human RefSeq mRNA sequences and collect the full insert sequence. Therefore, the full-length cDNA strategy was adopted to sequence and analyze a collection of 2000 cDNA sequences from placental cDNA library of a rhesus monkey. Full-length cDNA of the rhesus monkey will be beneficial for performing future genetic and biomedical studies.
Rhesus monkey placenta was harvested and used to generate a normalized, directional cDNA library. Around 2000 clones were randomly picked from the cDNA library and subjected to single-pass 5' sequencing using the cytomegalovirus primer located up-stream of the vector backbone. After trimming low-quality and vector sequences and removing contaminant host sequences, a total number of 1835 high-quality (quality score > 20) ESTs were obtained with a mean length of 858 bp. The length distributions of the ESTs are shown in Figure Figure1.1. cDNA length ranged from 170-1174 bp with an average of 858 bp. These average lengths were likely limited by sequencing technology. All cDNA sequences have been deposited in GenBank with continuous accession number of FS722297-FS724151.
The cDNA library derived from rhesus monkey placenta was constructed by the oligo-capping method. Two thousand sequences of rhesus monkey placental cDNA were annotated by the BLAST program. Since RefSeq sequences contain partially overlapped isoforms, non-redundant RefSeq sequences were constructed based on the Entrez Gene database. Consequently, 165 vector sequences that were included in the raw sequence data were eliminated. To classify these rhesus monkey cDNAs and find their human homologues, BLAST searches were performed to human RefSeq databases. Of the 1835 cDNA sequences, 1648 were homologous to the human RefSeq gene set and were clustered into 1462 types of genes, indicating that the cDNA sequences would cover approximately 4.8% of the known human genes (Table (Table1).1). Using the protein coding gene set of the rhesus monkey cDNA as defined by human RefSeq mRNA database, the frequency of mapping in 5'untranslated repeat (UTR), 5'UTR_CDS, CDS, CDS_3'UTR, 3'UTR, and 5'UTR-CDS-3'UTR was investigated at various distances from genes. Of the annotated 1648 cDNA transcripts, only about 86% of these (1590 transcripts) were present within the known gene regions: 20 mapping in 5' UTR, 143 in 5'UTR_CDS, 142 in CDS, 549 in CDS_3'UTR, 532 in 3UTR, and 204 in 5'UTR-CDS-3'UTR (Table (Table1).1). Of those that matched human RefSeq mRNAs, 204 cDNA sequences contained the full coding region with 5'UTR and 3'UTR sequences. Although the oligo-capping cDNA library construction method was aimed to generate full-length cDNA sequences, the results indicated that only 367 transcripts were derived by 5'UTR sequences. This relative low percentage of full-length inserts was mainly due to the fact that the method used was not optimized to generate a full-insert cDNA library.
Another study reported the initial sequencing and comparative analysis of rhesus monkey cDNA sequences from 11 tissues. These 48,642 sequence data from three different macaque species represented an initial sampling of the putative rhesus orthologs for 6216 human genes, and the researchers focused on the genetic divergence between the human and non-human primate . Because their cDNA libraries were constructed from the poly (dT)-primed cDNA, their method for Uni-ZAP cDNA library construction could not aim at the full-length cDNA sequences. However, the present oligo-capping method with normalization could capture non-redundant full-length mRNA sequences. To declare the differences between the present and previous  data, the prior results were reanalyzed. Although a total of 48,642 sequence reads from 11 rhesus monkey tissues had been sequenced, the present reanalysis retrieved and analyzed placenta tissue data sets of 12,033 sequences. Of these sequences, 8340 cDNA sequences corresponding to 2390 human RefSeq genes (7.8% of all human RefSeq genes) were collected (Additional file 1). Intriguingly, only 284 genes overlapped with the previous analysis. Although the main target of placenta tissues is the same, their results showed quite different data sets. These different results reflect the gap in knowledge in the identification and analysis of rhesus monkey genes.
From the 1835 sequences, consensus sequences could be constructed to 138 rhesus monkey genes by aligning with at least two sequences of individual genes and the nucleotide sequence identity between humans and rhesus monkey. Sequence identities were calculated between 298 rhesus monkey consensus sequences and the corresponding human RefSeq mRNA sequences. These 298 consensus cDNA sequences of 138 rhesus monkey genes aligned with 5'UTR_CDS_3'UTR, 5'UTR-CDS, CDS, CDS_3'UTR and 3'UTR regions of human RefSeq mRNAs (Figure (Figure2).2). The average sequence identity of the 5'UTR_CDS in 14 cDNAs was 95.1%, 5'UTR_CDS_3'UTR in 42 cDNAs was 95.9%, CDS in 19 cDNAs was 96.8%, CDS_3'UTR in 104 cDNAs was 95.7%, and 3'UTR in 119 was 93.3%. The patterns of divergence were different from the 5'UTRs, CDSs, and 3'UTRs. The sequence divergence in the 5'UTRs and CDS region was significantly lower than that of 3'UTR sequences. These results coincided with a previous study indicating the sequence identity between human and rhesus monkey cDNA was 97.79% in coding sequence and 95.10% in 3'UTRs . Upstream and coding regions of functional genes are very important . Thus, these regions are evolutionary well-conserved in comparison with 3' UTR regions. Specifically, sequence substitution in the CDS region could disrupt the important functional domain or coding frames. Moreover, this substitution in the 5'UTR region also could interrupt the binding interaction between regulatory binding sites and regulatory elements.
Use of the present oligo-capping method enabled the construction of full-length-enriched cDNA libraries from rhesus monkey placenta tissue. Although various genomics projects have focused on sequencing of the genome or ESTs, full-length cDNA sequences are uniquely informative resources for accurately predicting the full spectrum of the transcriptome in specific species .
Of the 1835 placenta cDNA sequences, 187 sequences were not homologous to the human RefSeq mRNA sequences (Figure (Figure3).3). Although 66 transcripts are not annotated with human RefSeq mRNA sequences, their sequences matched with the non-RefSeq mRNA (human and rhesus) and ESTs (human and rhesus) sequences in the database. Finally, 121 transcripts were designated as unknown transcripts.
To characterize the 121 unknown transcripts, two individual strategies were established. One is the human genome aligning method with open reading frame (ORF) coding method (60 transcripts). The other uses only the ORF coding method (61 transcripts). Among the 121 transcripts, 60 transcripts matched with the human genome using BLAST (Table (Table2).2). Remarkably, these 60 transcripts also contained ORFs with lengths ranging from 44-198 amino acids (data not shown). These unknown transcripts were identified in known gene regions or intergenic region. Furthermore, their matching patterns in human genome were similar with splicing patterns. These matching regions were designated as "block". Although detailed characterization and experimental validation of these 60 transcripts was not done, they could be valuable sources for the novel gene candidates.
Among the 121 transcripts, 61 were analyzed only using ORF coding. Because these 61 transcripts did not share significant similarities with any human genome, they were anticipated to be rhesus monkey specific transcripts. However, due to the limitation of rhesus monkey genome availability, this analysis could not be done. These 61 unknown transcripts clearly contain ORF regions that average 106 amino acids in length (data not shown). These results should also prove interesting for the identification of lineage specific rhesus monkey transcripts.
The results of species specific transcripts indicated in the present analysis are consistent with recent comparative analysis between human and chimpanzee . These species specific transcripts could be informative clues for the explanation of different characters. The rhesus monkey is one of the most widely-used and valuable biomedical animal models for the investigation of numerous human diseases. This information would provide a better understanding of the genetic information of rhesus monkey species.
INDEL sequences of placenta expressed transcripts were analyzed between human and rhesus monkey. If a sequence was aligned in more than one place in a genome, only the high-scoring sequence pair alignments was kept to ensure that a sequence mapped to a single locus. We selected human and rhesus monkey gene regions bearing nucleotide INDELs when aligned to the genome. A total of 214 transcripts showed different transcript structures. Next, the frequency of INDELs was investigated in 5'UTR, 5'UTR_CDS, CDS, CDS_3'UTR, and 3'UTRs in putative rhesus monkey genes. Of the 214 INDELs regions, 200 were located in functional gene regions including 14 in 5'UTR, seven in 5'UTR_CDS, 70 in CDS, 11 CDS_3'UTR, and 98 in 3'UTR regions (Figure (Figure4).4). Moreover, the INDEL density in the 3'UTRs was significantly lower than the 5'UTR regions. INDELs could disrupt important motifs in regulatory regions and also alter the spacing between regulatory binding sites. The present analysis revealed low INDEL density in the 5'UTR region and high INDEL density in the 3'UTR region and, especially, the CDS region. These results may be explained by selection acting against INDELs in specific regions.
Presently, a screening procedure was developed to identify putative rhesus monkey specific exons with transposable elements in alternative splicing events. Exons were analyzed using rhesus monkey placenta cDNA sequences. The 214 candidates were manually inspected to narrow them to two highly plausible cases: exons that exhibited alternative splicing patterns in the rhesus monkey but not in humans. Moreover, some alternative splicing events were rhesus monkey specific: the rhesus monkey exon of the BCS1L gene was derived from rhesus monkey specific AluYRa2 elements (Figure (Figure5a).5a). AluYRa2 is commonly considered to be a rhesus monkey specific Alu element . In addition, the rhesus monkey specific exon of CCDC23 was derived from MER11B elements (Figure (Figure5b).5b). Intriguingly, two rhesus monkey specific exons were derived from the transposable elements of AluYRa2 (SINE family) and MER11B (LTR family). These two transposable elements are abundant in different genomic regions including intergenic regions and intron regions. The consensus TE element, specifically Alu elements, carries multiple sites that are similar, but not identical, to the real splice site . Therefore, these two elements could be frequently exonized as primate-specific exons and could thus be used to identify the BCS1L and CCDC23 genes showing rhesus monkey specific INDELs.
The present study demonstrated the evolutionary occurrence of species-specific exons by alternative splicing of transposable elements in the gene during evolution. Although an absolute determination of species specificity will require complete rhesus monkey genome sequencing or experimental validation, preliminary computational analysis could be done to test the presence or absence of these sequences in the human and other primate genomes. Several studies have characterized the series of mutations needed occur within a transposable element in order to generate a new exon . For example, it was described in the human RNA editing enzyme ADAR2, where exon 8 is a new Alu-derived primate-specific exon that is alternatively spliced in high inclusion levels  in a tissue-regulated manner. The new exon is inserted in the catalytic domain of ADAR2 and, while the exon-containing variant has the same substrate specificity as the original one, it has an altered catalytic activity . Moreover, 62% of new exons in human are associated with primate-specific Alu retroposons, and 28% of new exons in rodents are derived from rodent-specific SINEs . Therefore, it seems that, in primates, the transposable elements exonization mechanism is being used as a major source for acetated, lineage specific evolution, and is perhaps a key driving force to eventual speciation.
In the present study, a cDNA library was constructed using an oligo-capping method from the placenta of the rhesus monkey, and approximately 2,000 randomly picked normalized clones were sequenced. The 1835 rhesus monkey placenta full-length cDNA sequences described here significantly expands the molecular resources available for the genus. Further analysis revealed 121 transcripts in rhesus monkey cDNA, in which 61 did not share significant similarities with any human genome. Such sequence information was used in comparative analysis to identify novel genes specifically expressed in rhesus monkey. Moreover, some alternative splicing events are rhesus monkey specific: rhesus monkey exons of BCS1L and CCDC23 genes were derived from rhesus monkey specific AluYRa2 and MER11B elements, respectively. Increasing the genomic resources and information of rhesus monkeys will greatly contribute to the development of evolutionary biology and biomedical sciences. The construction of a large scale collection of full-length cDNA sequence from rhesus monkey placenta tissue and homology searches in databases would facilitate the discovery of novel genes.
The placenta sample was collected from an 8-year-old female rhesus monkey of Chinese origin during cesarean delivery. All animal housing and experiments were performed in accordance with Korea Research Institute of Bioscience and Biotechnology (KRIBB)Institutional Animal Care and Use Committee Guidelines (Accepted No. KRIBB-AEC-09017).
Collected placenta tissue was immediately frozen in liquid nitrogen and used for RNA extraction. Modified oligo-capped cDNA libraries were constructed according to a previously described method . After the construction of the full-length enriched cDNA library, normalization steps were carried out as previously described . During all steps for full-length cDNA library construction and normalization, a total of five steps were checked (RNA isolation step, first cDNA synthesis step, second cDNA synthesis step, transformation step, and normalization step). All these procedures were conducted by CoreBioSystem (Korea).
The cDNA clones were sequenced with ABI 3730 automated sequencers. Sequencing of size-selected 2000 clones was determined by a commercial sequencing company (Cosmo Genetech). For the sequencing of 5' region of full-length cDNA sequences, a cytomegalovirus primer (5' CGC AAA TGG GCG GTA GGC GTG 3') was used. The rhesus monkey cDNA sequences were deposited in the public DNA databases [DDBH/EMBL/GenBank: FS722297-FS724151].
Human genome build 37.1, NCBI RefSeq mRNA (12 March 2009), and the June 2006 NCBI rhesus build 1 genome were used as the reference databases for all analyses. Transposable elements in the rhesus monkey genome sequences were identified by RepeatMasker http://repeatmasker.genome.washington.edu, and transposable element consensus sequences were identified by Repbase Update .
A total of 2000 cDNA sequences were selected. cDNA sequences were first base-called using a modified version of the phred algorithm  and then screened for cloning vector, lambda-phage, and Escherichia coli contamination using the cross_match program . Sequences exhibiting multiple cloning sites or any contamination with lambda-phage or E. coli sequence were excluded from further analysis . The result was the generation of a clean, high-quality EST sequence set. After trimming low-quality and vector sequences and removing contaminant host sequences, a total number of 1835 high-quality ESTs were obtained with a mean length of 858 bp. The BLAST program was used to align each rhesus monkey cDNA sequence with the human RefSeq mRNA sequence. Whenever BLAST failed to align rhesus monkey cDNA sequence with human RefSeq mRNA sequence, it was compared to the rhesus monkey genome database using the BLASTN program . The rhesus monkey sequences that matched the sequences of human RefSeq mRNAs were defined as the sequences of putative rhesus monkey genes. The rhesus monkey cDNA sequences were then mapped on the draft genome sequence of the rhesus monkey.
CDS: coding sequence; EST: expressed sequence tag; ORF: open reading frame; UTR: untranslated region;
DS Kim analyzed contents of this paper and drafted the manuscript. JW Huh designed the experiment on the whole study and revised the manuscript. YH Kim and SJ Park performed the experiments and analyzed the data. KT Chang participated in its analysis and provided essential direction.
All authors read and approved the final manuscript.
Rhesus monkey placenta cDNA sequences mapping with human RefSeq mRNA transcripts.
This study was supported by a research grant from the Ministry of Education, Science and Technology (KBM4311022, KGC5401011), Republic of Korea.