|Home | About | Journals | Submit | Contact Us | Français|
Conceived and designed the experiments: BP CL TNP. Performed the experiments: BP CL TNP. Analyzed the data: BP CL TNP. Contributed reagents/materials/analysis tools: BP CL TNP. Wrote the paper: BP CL TNP.
β-turns are the most common type of non-repetitive structures, and constitute on average 25% of the amino acids in proteins. The formation of β-turns plays an important role in protein folding, protein stability and molecular recognition processes. In this work we present the neural network method NetTurnP, for prediction of two-class β-turns and prediction of the individual β-turn types, by use of evolutionary information and predicted protein sequence features. It has been evaluated against a commonly used dataset BT426, and achieves a Matthews correlation coefficient of 0.50, which is the highest reported performance on a two-class prediction of β-turn and not-β-turn. Furthermore NetTurnP shows improved performance on some of the specific β-turn types. In the present work, neural network methods have been trained to predict β-turn or not and individual β-turn types from the primary amino acid sequence. The individual β-turn types I, I', II, II', VIII, VIa1, VIa2, VIba and IV have been predicted based on classifications by PROMOTIF, and the two-class prediction of β-turn or not is a superset comprised of all β-turn types. The performance is evaluated using a golden set of non-homologous sequences known as BT426. Our two-class prediction method achieves a performance of: MCC =0.50, Qtotal=82.1%, sensitivity =75.6%, PPV =68.8% and AUC =0.864. We have compared our performance to eleven other prediction methods that obtain Matthews correlation coefficients in the range of 0.17 – 0.47. For the type specific β-turn predictions, only type I and II can be predicted with reasonable Matthews correlation coefficients, where we obtain performance values of 0.36 and 0.31, respectively.
The NetTurnP method has been implemented as a webserver, which is freely available at http://www.cbs.dtu.dk/services/NetTurnP/. NetTurnP is the only available webserver that allows submission of multiple sequences.
The secondary structure of a protein can be classified as local structural elements of α-helices, β-strands and coil regions. The latter is often thought of as unstructured regions, but do contain ordered local structures such as α-turns, γ-turns, δ-turns, π-turns, β-turns, bulges and random coil structures , . Turns are defined by a distance that is less than 7 Å between Cα-atoms i, i+2 for γ-turns, i, i+3 for β-turns, i, i+4 for α-turns and i, i+5 for π-turns. Within each turn class, a further classification can be made based on the backbone dihedral angles phi and psi.
β-turn types are classified according to the dihedral angles (Φ and ψ) between amino acid residues i+1 and i+2 , . The standard nomenclature for the β-turn types are: I, I', II, II', VIII, VIa1, VIa2, VIb and IV . The dihedral angles for the 9 turn types are shown in Table S1.
A β-turn thus involves four amino acid residues, where the two central residues, i+1 and i+2, cannot be helical. Occasionally β-turns are stabilized with a hydrogen bond between the N-H of residue i and the C=O of residue i+3. In cases where no hydrogen bond is found, the β-turn is referred to as an open β-turn .
β-turns are the most abundant type of turn structure found in proteins. They play an important role in the formation of compact shapes in proteins, and are often referred to as orienting structures due to the fact that they have the ability to reverse the direction of a protein chain. Approximately 25% of amino acids in protein structures are located in a β-turn and about 58% of all β-turns are composed of different overlapping β-turn types .
Prediction of β-turns started in the 1970s where the first β-turn prediction methods relied on statistical information derived from three-dimensional protein structures , , , , , . The method implemented by Zhang and Chou  considered the pairing of the first and the fourth residue, and of the second and the third residue in a β-turn, and the predictive performance reached a Matthews correlation coefficient of 0.17. The work by Fuchs and Alix  used statistical methods combined with information obtained from regular secondary structure prediction. Combined with propensity scores and use of evolutionary information, they achieved a Matthews correlation coefficient of 0.41.
The most accurate β-turn predictors today utilize machine-learning methods, although the first approaches did not reach the performance obtained by the best statistical methods. The first method that predicted β-turns by use of neural networks was implemented by McGregor et al.  achieving a Matthews correlation coefficient of 0.20. Ten years later Shepherd et al.  added secondary structure predictions and the use of a two-layered network architecture (BTPRED method) and obtained a Matthews correlation coefficient of 0.35. Using a k-nearest-neighbor approach, a method by Kim  reached a Matthews correlation coefficient of 0.40. Kaur et al. ,  further enhanced the performance of β-turn prediction by use of secondary structure predictions and evolutionary information in form of position specific scoring matrices as input to the neural networks (BetaTPred2 method) . Using a uniform dataset of 426 non-homologues proteins (BT426) they obtained a Matthews correlation coefficient of 0.43. Recently support vector machines have become more widely used in the field of β-turn prediction, which is seen by the work of Zhang et al.  and Liu et al. (E-SSpred method) . Using support vector machines with multiple alignments and secondary structure predictions from PSIPRED , Zhang et al. obtained a Matthews correlation coefficient of 0.45, which was slightly higher than the E-SSpred method. E-SSpred reached a Matthews correlation coefficient of 0.44, but they were the first to break the 80% accuracy (Qtotal) barrier and achieved a Qtotal of 80.9%, compared to 77.3% by Zhang et al.
Zheng and Kurgan  applied support vector machines using a feature space consisting of position specific scoring matrices and secondary structure predictions from four different methods. After feature reduction, using 90 features, they obtained a Matthews correlation coefficient of 0.47. A similar performance was reached by Hu and Li  with a method based on support vector machines using features from position conservation scoring functions. Their method obtained a Matthews correlation coefficient of 0.47 using 7-fold cross-validation on the BT426 dataset.
β-turns are often accessible and generally hydrophilic, two characteristics of antigenic regions . For this reason they are suitable candidates for being involved in molecular recognition processes. Pellequer et. al.  found that 50% of the linear B-cell epitopes in a small dataset of 11 proteins were located in turn regions. Thus prediction of β-turns could improve the prediction of epitopes. Krchnak et al.  found that the parts of a protein, which can induce protein-reactive anti-peptide anti-bodies, mostly reside in regions that have a high tendency to form β-turns. A more recent article by the same authors showed that peptide sequences including a β-turn conformation tended to induce antibodies that were able to cross-react with the parent protein . β-turn and coil conformations has also previously been used to predict linear epitopes . Furthermore, β-turn types I and II, are important for binding between phospho-peptides and SH2-domains .
NetTurnP is a new method trained to predict β-turns and the corresponding β-turn type using two layers of neural networks. An improved performance is shown compared to other prediction methods. It has been implemented as a webserver, which is freely accessible at http://www.cbs.dtu.dk/services/NetTurnP/.
A schematic overview of the final NetTurnP method is shown in Figure 1. The method consists of two artificial neural network layers. Several second layer network setups were tested in order to find the architecture with the highest cross-validated MCC value based on training set sequences. These different setups can be seen schematically in Table S1. The setups gave similar performances as seen in Figure 2, however, we chose the best setup (M) for the final NetTurnP method.
Classification artificial neural networks, β-turn-G, were trained to predict whether or not an amino acid was located in a β-turn. Input to the networks was sequence profiles in form of PSSM's, predicted secondary structure and surface accessibility. Using 10-fold cross validation spanning a series of different network architectures, an ensemble was constructed of the best 100 network architectures, determined by cross validation leave-out tests (see methods). A cross-validated test performance of Qtotal=77.8%, PPV =51.3%, Sens =73.1%, MCC =0.47 and an AUC of 0.846 was obtained.
Furthermore, position specific networks, β-turn-P as described in materials and methods, were also trained in order to increase the predictive performance of the second level networks. Test performances for these networks can be seen in Table S2.
The output from the first layer networks was used as an input to the second layer networks. The final method uses predictions from the β-turn-P and β-turn-G networks, including secondary structure and relative surface accessibility predictions from NetSurfP . An ensemble of 10 network architectures was selected corresponding to the top ranking network architecture within each of the subsets, based on the leave-out performance. Further increasing the number of architectures in the ensemble did not increase the performance (Figure S1). A cross-validated test performance of Qtotal =78.8%, PPV =53.0%, sensitivity =71.5% and an MCC of 0.48 with an AUC of 0.849 was obtained. Results for both the first and second layer network test performances are shown in Table S3. All performances increased from the first to the second layer networks, except for the sensitivity, which decreased 1.6 percentage points.
The neural network ensemble was also evaluated against the BT426 dataset. The performance values achieved were: Qtotal=78.2%, PPV =54.4%, sensitivity =75.6% and a MCC of 0.50 with an AUC of 0.864. The ROC curve for the evaluation of the NetTurnP is shown in Figure 3. A 7-fold cross validation performed on the BT426 dataset showed that the result obtained is very comparable to the general NetTurnP method as can be seen in Table 1.
The Qtotal measure can be optimized, but at the expense of a lower MCC and sensitivity. We analyzed this relationship by varying the cut-off for a positive prediction as seen in Figure 4. A cut-off of 0.61 gave the highest Qtotal of 82.5% and MCC of 0.46 on the test set, whereas using our default cut-off (0.50) gave a Qtotal of 78.8 and an MCC of 0.48.
Using this cut-off of 0.61 for the evaluation dataset resulted in a Qtotal of 82.1% and an MCC of 0.48 as shown in Table 1.
Predicted and assigned β-turns are illustrated on the PDB structure 2WNS:A in Figure 5. It is a transferase with 197 amino acids where 31 amino acids were assigned by PROMOTIF as being located in a β-turn. Prediction of β-turns was done using the NetTurnP and NetTurnP-tweak methods to show the effect of a tweaked Qtotal performance. The performance using NetTurnP on 2WNS:A gave Qtotal=87.3%, PPV =55.8%, sensitivity =93.6% with a MCC of 0.66 and an AUC of 0.955. Using NetTurnP-tweak the protein chain was predicted to a precision of Qtotal=89.3%, PPV =100%, sensitivity =32.3% with a MCC of 0.54. The AUC value was unchanged.
Classification networks were trained to predict whether an amino acid was located in one of the nine types of β-turn, as earlier defined. Networks were trained using the same method as described for β-turn-G i.e. an ensemble of 100 networks architectures for the first layer and 10 architectures for the second layer networks. Evaluation performances for the second layer β-turn-S networks are summarized in Table 2, along with a comparison against four other methods.
Sequences for each of the three datasets PLP399, PLP364 and PLP273 were submitted to the NetTurnP, NetTurnP-tweak and the BetaTPred2 webservers. Evaluation performances are summarized in Table 3.
Evaluating NetTurnP and NetTurnP-tweak showed that both methods are very stable over all three datasets, with only 0.22% difference in Qtotal for NetTurnP, and 0.15% for NetTurnP-tweak within the datasets. The same trend of stable prediction is seen for all other performance measures as well. NetTurnP and NetTurnP-tweak have a small decrease of 0.03 in MCC compared to the performance against BT426 (Table 1) whereas BetaTPred2 has an even bigger decrease of 0.06 in MCC. This indicates that both of the NetTurnP methods are still better than the BetaTPred2 method and now by an even bigger margin. Also, the slightly reduced MCC values indicate that the new PLP datasets contain more difficult targets compared to the original BT426 dataset,
In the work presented in this paper a neural-network method called NetTurnP was developed. It predicts β-turns in general and the specific type of β-turn. This work represents one of the few studies where an independent evaluation dataset was used in addition to cross-validation. The evaluation set was non-homologous to the training datasets used. NetTurnP reached a Qtotal of 78.2% with a MCC of 0.50, using a two-layered network structure, where the predictions from the first layer networks were used as input for the second layer.
β-turns tend to be located at solvent-exposed surfaces. Analyzing our training dataset (Cull-2220), we found that the most frequently observed amino acids in β-turns compared to the amino acid at any position were: Gly (11.6%/7.2%), Asp (8.9%/5.9%), Ser (7.1%/6.1%), Pro (7.0%/4.6%), Ala (6.4%/7.8%), Asn (6.3%/4.2%) and Glu (6.3%/7.0%). These amino acid residues are hydrophilic or small, where Pro is special due to its fixed and rigid structure making it suitable to reverse the direction of a protein chain. It is seen that Gly, Asp, Ser, Pro and Asn are occurring more often in β-turns than in general, and that Ala and Glu occur less frequently. A complete table of the frequencies for all amino acids is shown in Table S4.
For the second layer networks different setups were tested in order to find the highest test (MCC) performance. We found that it was most optimal to use predictions from the networks β-turn-G and β-turn-P and with inclusion of predicted secondary structure and relative surface accessibility predictions.
The second layer networks were found to filter out the noise and increase the AUC value from 0.846 to 0.849 in test performance. This increase was found to be a significant increase corresponding to a p-value << 0.001, using an unpaired test with two independent samples . For the evaluation dataset BT426 the AUC increased from 0.860 to 0.864 after primary and second level networks, respectively. (p-value << 0.001).
Because of the unbalanced dataset (25% β-turns), Qtotal is a poor measure by itself, as it is possible to achieve a Qtotal of 75% if all residues were predicted to be non-β-turns. Instead, NetTurnP was trained to achieve the best MCC, which will also balance the performance measured on sensitivity and specificity. The effect of a tweaked Qtotal performance (NetTurnP-tweak) showed that we could obtain a better Qtotal than any other method, but at the expense that more false and true positives are removed as seen in Table 1 and Figure 6. Therefore only the most confident predictions remain, but the method becomes less sensitive. NetTurnP, with tweaking Qtotal, achieves the best MCC performance of 0.48 compared to other methods.
For the prediction of specific β-turn types NetTurnP showed improved performance for four out of six β-turn types compared to other methods as seen in Table 2. We do provide a prediction via the webserver for the β-turn types VIb, VIa1 and Via2, even though the performances are quite low with MCC values of 0.11, 0.07 and 0.03 respectively. It is most likely due to the scarce number of these β-turn types.
Three new datasets were created with the purpose of evaluating NetTurnP and NetTurnP-tweak against a more recent set of sequences than the original dataset BT426. For the comparison NetTurnP/NetTurnP-tweak, DEBT, MOLEBRNN and BetaTPred2 were chosen. Due to errors in the DEBT and MOLEBRNN webservers, we were not able to obtain enough results for a comparison. MOLEBRNN never completed any calculations, and DEBT only succeeded to return a few results. All sequences were successfully submitted to NetTurnP/NetTurnP-tweak and BetaTpred2. Multiple sequences can be submitted to the NetTurnP webserver, which is a functionality that none of the other webservers provide.
For NetTurnP/NetTurnP-tweak the performance drops by 0.03 in terms of MCC compared to the performance obtained using the BT426 dataset. BetaTPred2 had an even bigger decrease in MCC of 0.06. This could indicate that the newer sequence data is a more challenging dataset.
A dataset of 75 experimentally determined antigen-antibody structures with predicted epitope residues was downloaded from the supplementary section of DiscoTope  in order to analyze the frequency of β-turns in discontinuous B-cell epitopes. We find that there is an overrepresentation with a factor 2 (data not shown) of β-turns in the discontinuous B-cell epitopes. We therefore believe that prediction of β-turns in general, can further improve immunological feature predictions.
To evaluate the NetTurnP method, a dataset of 426 non-homologous protein chains was used. The dataset, commonly known as BT426, was created by Guruprasad and Rajkumar  and consists of >94,800 amino acids. Several groups use it as a golden set of sequences upon which performance values are reported and compared. The dataset consists of protein chains whose structure has been determined by X-ray crystallography at a resolution of 2.0 Å or better. Each chain contains at least one β-turn region. In total 23,580 amino acids, corresponding to 24.9% of all amino acids, have been assigned to be located in β-turns. None of the sequences in the dataset share more than 25% sequence identity. The BT426 dataset was downloaded from the Raghava Group's website: http://www.imtech.res.in/raghava/bteval/dataset.html. Four sequences are obsolete from PDB and superceded by newer sequence data. Therefore 1GDO.A was replaced by 1XFF.A, 5ICB by 1IG5, 1ALO by 1VLB and 3B5C by 1CYO. This dataset was solely used for the final evaluation of our NetTurnP method.
Three new datasets were constructed with the purpose of evaluating the NetTurnP method against a more recent set of protein sequences. Protein sequence data was extracted from the RCSB (Research Collaboratory for Structural Bioinformatics) Protein Data Bank (PDB)  using the protein culling server PISCES . An initial dataset was created using the following criteria: Maximum sequence identify <=25%, Resolution <=2.0 Å, R-factor <=0.2, sequence in the range 25 -10,000 amino acids and including X-structures only. The resulting dataset contained 3,572 PDB protein chains before homology reduction. A Hobohm1 algorithm with a threshold as described previously  was used to create the final homology reduced dataset PLP399, containing 399 protein chains. No sequences in the dataset share more than 25% sequence identity to a sequence within the BT426 dataset, Cull-2220 dataset (described below) or the datasets used for training and evaluation of the NetSurfP method . The PLP399 dataset was further subdivided into PLP364 containing only sequences with deposition date from 2008 and newer and PLP273 containing sequences from 2009–2010. All three datasets are solely used to evaluate the NetTurnP method, and they are available for download at http://www.cbs.dtu.dk/services/NetTurnP/suppl/plp.php.
Protein sequence data was obtained from PDB using PISCES. A dataset was constructed in two steps, first an initial selection of potential sequences and later a more strict selection based on a Hobohm1  homology reduction algorithm. First PDB was culled using the following criteria: Maximum sequence percentage identity <=40%, Resolution <=3.0 Å, R-factor <=0.2, sequence length in the range 40–10,000 amino acids and including X-ray structures only. The resulting dataset contained 5,648 PDB protein chains before homology reduction. An empiric sorting function (1) was applied to rank the protein chains such that high-resolution structures with the most experimentally determined amino acids were preferred instead of the shorter low-resolution homologous protein sequence. A Hobohm1 algorithm with a threshold as described previously  was used to create the final homology reduced dataset (Cull-2220). No sequences in the dataset share more than 25% sequence identity to a sequence within the BT426 dataset.
Equation 1 – Ranking of experimentally determined protein sequences. The best rank is assigned to the protein sequence with the lowest score. “resolution” is the resolution in Ångstroms according to PDB, “sequence_length” is referring to the actual length of the sequence which may include amino acids for which there are no available coordinates in the PDB-file. “pdb_length” is the length of the sequence for which there are coordinates for the amino acids.
The program PROMOTIF  was used for assignment of β-turns and for the Cull-2200 dataset where 98,624 out of 451,812 amino acids were assigned to be inside a β-turn (21.8%) region.
According to restraints on phi(Φ) and psi(ψ) dihedral angles between residues i+1 and i+2, nine β-turn type specific datasets were created. The Φ, ψ restrains for each of the types (I, I', II, II', IV, VIII, VIb, VIa1 and VIab) are shown in Table S5. These angles are allowed to deviate ±30° from the defined angles, with the addition that one dihedral angle is allowed to deviate as much as ±40°. Type VIa1 and VIa2 also require a cis-proline at position i+2.
For the general prediction of β-turns, the positive set includes the amino acid residues that belong to any of the 9 β-turn types and the negative set include all other residues. For the type specific β-turn predictions, the positive sets were reduced to include only β-turns of one specific type whereas everything else comprised a negative dataset. The number and percentage of amino acids (positive sets) in each of the type specific datasets are: type I 40,482/9.0%, type I' 4,812/1.1%, type II 14,375/3.2%, type II' 3,124/0.7%, type IV 38,445/8.5%, type VIII 11,192/2.5%, type VIb 1,120/0.3%, type VIa1 736/0.2% and type VIa2 214/0.1%.
Sequence profiles i.e. Position-Specific Scoring Matrices (PSSM) were generated for all protein chains, using the iterative PsiBLAST program . Query sequences were blasted for four iterations against a local copy of the National Center for Biotechnology Information (NCBI) non-redundant (nr) sequence database, which for speed purposes had been homology-reduced using CDHIT  to less than 70% sequence identity. An E-value cut-off of 1×10-5 was used.
Secondary structure and surface accessibility predictions were generated for all protein chains, using the NetSurfP program .
A standard feed-forward procedure was utilized to train the neural networks , and a gradient descent method was used to back-propagate the errors where-after weights were updated . A sliding window of amino acids was presented to the neural network and predictions were made for the central position. The neural networks were trained using window sizes of 5, 7, 9, 11 and 13, the following number of hidden units: 50, 75, 100 and 125, and two output neurons. Altogether we used 20 different neural network architectures. A 10-fold cross-validation procedure was used, thus a total of 200 neural networks. Synapse weights were stored for the epoch where the best test set Matthews correlation coefficient was obtained.
Amino acids were encoded both using PSSM values, three neurons for predicted helix, strand and coil and one extra neuron for the relative surface accessibility, thus a total of 25 neurons were used to describe an amino acid.
Three different types of artificial neural networks have been trained:
The β-turn-G (G for general) method predicts if an amino acid is located in a β-turn region or not.
The β-turn-S (S for specific) method was trained to predict if an amino acid belongs to any of the nine β-turn classes: I, I', II, II', IV, VIII, VIb, VIa1 and VIab.
The method β-turn-P (P for position) is a combination of four sub-methods that have been trained to predict if an amino acid is located at position 1, position 2, position 3 or position 4 in a β-turn. Some amino acids can be assigned to multiple positions within a β-turn as shown in Figure 1. However, within each of the four sub-methods only one position was considered.
We found that the performance of the methods β-turn-G and β-turn-S could be improved by use of a second layer of neural networks where information from the β-turn-P method was included as input. A second layer is often used as some of false predictions can be corrected ,  and is due to the fact that new or enriched input data is provided for the second layer neural networks.
The quality of the predictions was evaluated using six measures; Matthews correlation coefficient  (MCC), QTotal, Predicted Positive Value (PPV), sensitivity, specificity and Area under the Receiver Operating Curve  (AUC). FP = False Positive, FN = False Negative, TP = True Positive, TN = True Negative.
Matthews correlation coefficient can be in the range of −1 to 1, where 1 is a perfect correlation and -1 is the perfect anti-correlation. A value of 0 indicates no correlation.
Qtotal is the percentage of correctly classified residues, also called the prediction accuracy.
PPV is the Predicted Positive Value, also called the precision or Qpred.
Sensitivity is also called recall or QObs, and is the fraction of the total positive examples that are correctly predicted.
Specificity is the fraction of total negative examples that are correctly predicted.
The above-mentioned performance measures are all threshold dependent and in this work a threshold of 0.5 was used, unless otherwise stated.
AUC is a threshold independent measure, and was calculated from the ROC curve which is a plot of the sensitivity against the False Positive rate = FP/(FP + TN). An AUC value above 0.7 is an indication of a useful prediction and a good prediction method achieves a value >0.85 .
setups tested for training in the second layer networks.
The table is listing the different setups tested for training in the second layer networks. In the table abbreviations are as follows: β-turn-G = β-turn/not-β-turn prediction from first layer networks, β-turn-P= position specific predictions from first layer networks, sec-rsa = secondary structure and surface accessibility predictions from NetSurfP , PSSM = Position Specific Scoring Matrices.
test performance for the first layer β-turn-P networks.
Test performances from the first layer β-turn-P networks using the Cull-2220 dataset. All performance measures have been explained in the methods section. All β-turn-P networks were trained using pssm + sec + rsa, where pssm = Position Specific Scoring Matrix, sec = Secondary structure predictions , rsa = Relative solvent accessibility predictions . The positions in the four network trainings are referring to the position in a β-turn.
Test performances from the first and second layer β-turn-G networks using the Cull-2220 dataset. All performance measures have been explained in the methods section. The first layer networks were using pssm + sec + rsa, and the secondary networks were using β-turn-P + β-turn-G + sec + rsa, where the used nomenclature are: pssm = Position Specific Scoring Matrix, sec = secondary structure predictions , rsa = relative solvent accessibility predictions . β-turn-G = β-turn/non-β-turn predictions, β-turn-P = predictions from the position specific networks.
Amino acid statistics in Cull-2200 dataset.
Frequencies for amino acids in β-turns and the Cull-2220 training set. The first part of the table ‘β-turn statistics’ shows the amount of residues, which have been assigned as β-turns and their percentage of the total amount of β-turn assigned residues in the Cull-2220 set. The second part of the table ‘Amino acid statistics’ shows the amount of residues and the percentage of the total Cull-2220 set.
Dihedral angles for the β-turn types as used by PROMOTIF
Dihedral angles for the β-turn types between residues two (i+1) and three (i+2) as used by PROMOTIF . These angles are allowed to deviate by ±30° from the defined angles, with the addition that one dihedral angle is allowed to deviate as much as ±40°. Type IV is used for all β-turns, which do not fall within the dihedral angle ranges for the eight defined types. Type VIa1, VIa2 also require a cis-proline at position i+2.
Matthews correlation using different setups and an increasing number of trained network architectures.
The figure shows test performances in Matthews's correlation coefficient when including an increasing number of trained networks architectures, named Top ranked network architectures, based on test set performance using different setups. Abbreviations for the setups are as follows: β-turn-P = position specific first layer predictions, β-turn-G = general β-turn/not-β-turn first layer predictions, sec-rsa = secondary structure and surface accessibility predictions from NetSurfP , PSSM = Position Specific Scoring Matrices. The setups are composed as follows: A= PSSM + sec-rsa, B=PSSM + β-turn-G+ sec-rsa, C= PSSM + β-turn-G, D= PSSM + β-turn-P, E= β-turn-P, F= β-turn-G + sec-rsa, G= β-turn-G, H= PSSM + β-turn-P + sec-rsa, I = β-turn-P + sec-rsa, J = PSSM + β-turn-P + β-turn-G + sec-rsa, K= PSSM + β-turn-P + β-turn-G, L= β-turn-P + β-turn-G, M= β-turn-P + β-turn-G + sec-rsa.
We would like to thank Ramneek Gupta with his help to proofread this manuscript.
Competing Interests: The authors have declared that no competing interests exist.
Funding: These authors have no support or funding to report.