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Contributions: IC and ATV contributed equally to this research and should both be considered first author.
A procedure that could allow an early in vivo and non-invasive detection of vulvar lesions would be extremely useful. We tested an innovative optical method (Optiprobe), which uses a harmless, visible light source for the in vivo, on-line detection of minimal alterations in the structure of vulvar epithelium. A group of 3 female volunteers without gynecological symptoms were first screened to evaluate optical properties of normal vulvar tissue. Next, a group of 16 patients undergoing gynecological examination for vulvar lesions was evaluated by the Optiprobe at suspected sites before these sites were biopsied for histological analysis. Adjacent, non-involved sites were also measured to provide internal controls. Histological analysis of the biopsies identified one case that did not show obvious alterations, 4 cases of high-grade vulvar intraepithelial neoplasia (VIN), 5 cases of vulvitis, and 6 cases of lichen sclerosis (LS).
The optical properties of the VIN cases were significantly different from those of controls, due to a decrease in the absorption spectra and an increase in the scattering spectra. In contrast, a significant increase in the absorption spectra and a decrease in the scattering spectra were observed in the cases of vulvitis. In the LS cases, the absorption spectra were as in controls, whereas the scattering spectra were significantly decreased. We conclude that the Optiprobe provides a useful tool for a rapid and non-invasive detection of vulvar alterations. The method should contribute to reduce the number of biopsies and to facilitate the long-term follow-up of vulvar lesions.
Vulvar squamous cell carcinoma of the vulva (SCC) accounts for about 4% of all gynecological malignancies.1 It has an incidence of about 1.8 per 100,000, which peaks at approximately 20 per 100,000 after the age of 75, making it as common as cervical carcinoma.2,3 SCC is usually slow growing and begins as a pre-malignant lesion, referred to as vulvar intraepithelial neoplasia (VIN).4 This lesion actually comprises a spectrum of epithelial changes that range from mild to severe dysplasia, which immediately precede invasive carcinoma. Although advanced VIN lesions may appear as variably pigmented patches under vulvoscopy investigation, they may be difficult to distinguish from a variety of benign diseases. This difficulty often leads to delayed diagnosis, until the obvious stage of invading squamous cell carcinoma.5 Presently, the standard procedure for definitive diagnosis consists in performing a biopsy of all suspected areas, even though only a fraction of these are at risk of neoplastic transformation.6 Because vulvar sampling is painful and bears infectious risks for the patient, an alternative method that would allow for a non-invasive diagnosis would be of great value in the screening programs of vulvar cancer.
Light-scattering spectroscopy is a recently developed optical method that relies on the in situ measurement of the light back-scattered (reflectance) from an illuminated tissue. The two underlying ideas are that light propagation, and thus reflectance, is modified under conditions altering the structure and/or composition of a tissue,7–11 and that optical coefficients can be derived from reflectance values, which provide information on these alterations.12–14 Thus, the absorption coefficient, which is thought to reflect the concentration of chromophores, and the scattering coefficient, which is thought to reflect the size and numerical density of cells and organelles, significantly differ in normal and pathological tissues.15–18
We have built an optical set-up, referred to as Optiprobe, which comprises a multi-fiber mini-probe to screen small tissue volumes of about 1 mm3 .17,19 We have found that this equipment allows for an in vivo, real-time detection of minimal tissue lesions, like those expected at the beginning of inflammatory and cancerous alterations.15,20 Here we have assessed whether the method has a sufficient sensitivity to identify alterations of vulvar tissues in patients undergoing vulvoscopy. We report that the Optiprobe method discriminated normal and pathological cases, with a sensitivity and specificity that compared well with those of histological analysis, while avoiding the need of invasive biopsies.
The study was approved by the Committee for Ethics in Research of the University of Geneva Hospitals, and conducted according to the guidelines of this Committee. After informed consent, a first group of 3 healthy volunteers were screened with the Optiprobe technology to determine the optical properties of normal vulvar tissue. A second group of 16 patients, undergoing vulvoscopic examination for suspicion of vulvar lesions, were similarly evaluated.
The very same sites were then sampled for standard histological analysis. Sites of non-involved tissue from nearby regions were also measured to provide an internal control. The optical-biopsy combined procedure did not imply additional discomfort for the patients, inasmuch as the fiber-optic probe was gently applied on vulvar surfaces for illumination with a harmless visible light source. On average, the duration of the combined vulvoscopicoptical-biopsy procedure was about 10 min longer than that of the standard vulvoscopicbiopsy examination.
The optical mini-probe set-up, and the theory of the method used to collect reflectance signals and to derive optical coefficients, have been previously described.17,19,21,22 Briefly, the tip of the hand-held optical probe was applied on the vulvar epithelium. Once in place, a 400 msec pulse of white-light (wavelength range 480–950 nm) provided by a halogen lamp (Ocean Optics LS-1) was delivered through the source fiber of the probe. Intensity of reflectance, i.e. light backscattered from the illuminated tissue, was simultaneously recorded at various distances from the source, using the 10 collecting fibers of the probe. Collected reflectance was brought to a spectroscope (Jobin-Yvon CP 200), focused on a cooled CCD camera (Hamamatsu C7041) during an exposition time controlled by a mechanical shutter, and computer digitized in 16 bits (National Instruments PCI-MIO-16E-4). Reflectance measurements were repeated 6–8 times per site and averaged. In each patient, at least one suspicious and one control site were probed before the surgical biopsy.
Once the gynecological examination was completed, the acquired reflectance spectra were analyzed using a Monte Carlo simulation and a modified form of the Henyey-Greenstein phase function to compute an absorption and a scattering coefficient. These optical parameters are thought to reflect the interaction of light with chromophores and the changes of light propagation in small tissue volumes, respectively.17,19,21
Biopsies of the vulvar tissue, taken at the very same sites that were probed optically, were fixed in 10% formol and processed for standard histological analysis by an expert pathologist. In this study, each sample was assigned to a normal, lichen sclerosis, VIN or a vulvitis group.
Optical measurements from altered and control sites were compared using appropriate non-parametric statistics, as provided by the SPSS program (SPSS Inc., Chicago, USA). Sensitivity of optical analysis (hereafter referred to as tests) was estimated by dividing the number of true positive cases (i.e. cases showing positive tests in patients with abnormal histology) by the number of true plus false negative cases (i.e. cases showing normal tests in patients with abnormal histology). Specificity of tests was evaluated by dividing the number of true negative cases (i.e. cases showing normal tests in patients with normal histology) by the number of true negative cases plus false positive cases (i.e. cases showing altered tests in patients with normal histology).
The optical properties of normal vulvar tissue were first evaluated by recording the absorption and scattering spectra in 3 healthy volunteers, whose vulva was clinically normal. In each case, maximal light absorption was observed between 400 and 500 nm (Figure 1A), reflecting the normal presence of chromophores oxy and deoxy-hemoglobin within surface tissues. In the same volunteers, the scattering coefficient decreased as a function of increasing wavelength (Figure 1B), consistent with the light-scattering characteristics of surface tissues. Comparison between the recordings from the 3 volunteers revealed significant (p<0.001) differences in both absorption (Figure 1A) and scattering (Figure 1B) coefficients. These differences were represented by a variation coefficient varying from 25–140% for the absorption spectra (Figure 1C), and from 5–20% for the scattering spectra (Figure 1D).
Immediately after the optical measurements, a biopsy was taken at each of the 16 suspected sites that had been investigated. The histopathological scoring of these samples resulted in the identification of one site showing no detectable alteration, 4 sites showing high-grade VIN, 5 sites with vulvitis and 6 sites with lichen sclerosis.
Absorption and scattering coefficients were determined for wavelengths between 400 and 700 nm, in 16 sites suspicious of vulvar lesion and in nearby sites that appeared phenotypically not involved and were used as controls. Non-parametric statistics confirmed that the optical properties of VIN sites were significantly (p<0.001) different from those of controls, due to a decrease in the absorption coefficient (Figure 2A) and an increase in the scattering coefficient (Figure 3A). Thus, on average, the absorption and scattering coefficients of VIN cases were 50% and 40% those of controls (Figures 2D and and3D).3D). Taking these values as optical threshold for diagnosis, we calculated that the Optiprobe would detect VIN cases with a sensitivity of 75% (absorption) - 50% (scattering).
Compared to controls, sites of vulvitis featured significantly (p<0.001) increased absorption values (Figure 2B) and decreased scattering values (Figure 3B). When expressed as percentage of the corresponding control values, the average absorption and scattering values of vulvitis cases represented 100% and 20% of control values, respectively (Figures 2E and and3E).3E). Using these values, the test discriminated normal from vulvitis cases with a sensitivity of 80% for both absorption and scattering coefficients.
The absorption coefficients evaluated at sites of lichen sclerosis were not significantly different from controls for all investigated light wavelengths (Figure 2 C,F). In contrast, a significant (p<0.001) decrease in scattering spectra was observed (Figure 3C), resulting, on average, in a 20% of variation from control values (Figure 3F). Using this scattering variation as threshold optical limit, the diagnostic test differentiated lichen sclerosis from normal cases with a sensitivity of 66%.
We have developed a new optical procedure and equipment for a non-invasive, in vivo analysis of small tissue volumes. The procedure is based on the spectroscopic measurement of the reflectance of white light, combining wavelengths of 400–700 nm, from which various optical parameters can be derived.28,32,33 Among these parameters, scattering and absorption coefficients are of particular interest for medical diagnosis inasmuch as they are thought to provide information about changes in cell structure and organization within tissues, and in content of various molecules, respectively.35–38 We have already shown hat the determination of these optical coefficients enable minimal alterations of skin surface to be detected in an experimental murine model,26 and can also correctly evaluate human mucosa in patients undergoing gastroscopy for suspected gastritis alterations.20 Here, we have tested whether the procedure can also be used to detect alterations of the vulvar epithelium, including VIN, vulvitis and lichen sclerosis. We have further assessed whether the method has a sufficient sensitivity to become a useful complement of standard clinical diagnosis, while limiting the need for an invasive biopsy for histological diagnosis.
To address these questions, we have first studied the optical properties of normal vulvar tissue in 3 volunteer women. The major light absorption for 420–480 nm wavelengths featured the presence of hemoglobin, which corresponds to the main chromophore encountered by probing photons.23,24 Scattering spectra revealed a slow decrease correlated to the increase of wavelengths, as is usually observed with surface biological tissues.23,25 Although both absorption and scattering measurements had a comparable shape in all volunteers, we observed significant differences in their absolute values. These differences could not be attributed to variations of the optical set-up, which probed stable standards with a variation coefficient of less than 5% for absorption and scattering measurement. Thus, the variation coefficients observed for absorption (30–140%) and scattering (5–20%) measurements of normal vulvar tissue clearly resulted from inter-individual variation. While the reason of this variation remains to be determined (could this be due to hormonal status?) the data imply the necessity of having a proper internal control for each individual undergoing the Optiprobe analysis. We have followed this strategy to investigate a series of 16 patients undergoing vulvoscopy for evaluation of benign or pre-malignant vulvar lesions by comparing the measurements made at suspected sites to those of phenotypically not-involved sites that were used as internal controls. We found significant variations in scattering and/or absorption coefficients in all the three types of lesions that were investigated. While the nature of the biological parameters that affected the optical coefficients remains to be determined, this method provides a new tool for a rapid distinction between pathological and normal sites. The changes in absorption and scattering appeared specific for a given type of lesion, inasmuch as they were decreased or increased differentially in cases of VIN, vulvitis, and lichen sclerosis. The observation of these combined variations in a clinical setting should indicate the type of lesion. Our analysis of spectral variations between pathological sites and control counterparts also enabled us to determine the wavelengths allowing for the best detection of each type of lesion. The absorption and scattering coefficients estimated at these wavelengths determined a sensitivity of the approach of more than 75% for the detection of both VIN and vulvitis cases, and of 66% for the detection of lichen sclerosis. These values show that the Optiprobe instrument can detect a majority of vulvar lesions. Further refinements of the set-up, based on a combined analysis by spectroscopic and fluorescence methods or tissue imaging, should further improve its performance. Our analysis shows promising results for a future application of optical methods in the diagnosis of vulvar lesions. Improvement in the mathematical processing of the optical coefficients from the reflectance measurements may facilitate the routine, clinical use of the method.
To conclude, we report here on a novel optical method that efficiently detects vulvar lesions with a sensitivity that compares well with the standard biopsy-histology approach, while avoiding the tissue invasion required by the latter approach. This method should prove a useful complement to clinical diagnosis of vulvar lesion in directing the biopsy procedure, as well as in guiding invasive therapeutic acts (e.g. laser irradiation, excision) to only relevant areas of the vulvar surface.
the authors would like to gratefully thank Dr. Michele Follen at The University of Texas M.D. Anderson Cancer Center for her helpful comments about this manuscript.