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Indian J Ophthalmol. 2010 Nov-Dec; 58(6): 549–550.
PMCID: PMC2993996

Sabouraud dextrose agar for the diagnosis of fungal keratitis

Dear Editor,

I read the recent publication on diagnosis of fungal keratitis with great interest.[1] Das et al. concluded that “This study recommends use of blood agar or chocolate agar for diagnosis of both bacterial and fungal keratitis in situations with limited resources.”[1] Omitting sabouraud dextrose agar (SDA) seems to be a challenge. In microbiology, SDA is used as a standard for fungus study.[2] The use of few samples in this study should be carefully considered. In addition, some fungi, especially for Histoplasma spp. cannot be successfully cultured via blood or chocolate agars.[34]


1. Das S, Sharma S, Kar S, Sahu SK, Samal B, Mallick A. Is inclusion of Sabouraud dextrose agar essential for the laboratory diagnosis of fungal keratitis? Indian J Ophthalmol. 2010;58:281–6. [PMC free article] [PubMed]
2. Odds FC. Sabouraud(’s) agar. J Med Vet Mycol. 1991;29:355–9. [PubMed]
3. Procop GW, Cockerill FR, 3rd, Vetter EA, Harmsen WS, Hughes JG, Roberts GD. Performance of five agar media for recovery of fungi from isolator blood cultures. J Clin Microbiol. 2000;38:3827–9. [PMC free article] [PubMed]
4. Roberts GD, Washington JA., 2nd Detection of fungi in blood cultures. J Clin Microbiol. 1975;1:309–10. [PMC free article] [PubMed]

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