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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
From:
J Immunol. Author manuscript; available in PMC 2011 July 15.
Published in final edited form as:
J Immunol. 2010 July 15; 185(2): 818–821.
Published online 2010 June 18. doi: 10.4049/jimmunol.1000724

FIGURE 3

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L. monocytogenes DNA colocalizes with ASC specks. NLRP3/NLRC4DKO macrophages expressing ASC-V5 were infected with L. monocytogenes. A, Hoechst-stained WT or ΔLLO L. monocytogenes DNA (blue) and ASC (green). B, DAPI-stained or unstained L. monocytogenes DNA (blue) and ASC (green). C, DAPI-stained L. monocytogenes DNA (blue), L. monocytogenes cell wall (red), and ASC (green). Enlarged merged images of L. monocytogenes DNA with cell wall from (1) an intact bacterium and (2) a lysed bacterium are shown without ASC green overlay. Corresponding phase images are shown. Scale bars, 15 μm. Original magnification ×60.

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