L. monocytogenes DNA colocalizes with ASC specks. NLRP3/NLRC4DKO macrophages expressing ASC-V5 were infected with L. monocytogenes. A, Hoechst-stained WT or ΔLLO L. monocytogenes DNA (blue) and ASC (green). B, DAPI-stained or unstained L. monocytogenes DNA (blue) and ASC (green). C, DAPI-stained L. monocytogenes DNA (blue), L. monocytogenes cell wall (red), and ASC (green). Enlarged merged images of L. monocytogenes DNA with cell wall from (1) an intact bacterium and (2) a lysed bacterium are shown without ASC green overlay. Corresponding phase images are shown. Scale bars, 15 μm. Original magnification ×60.