Geminin deletion affects all 3 hematopoietic lineages.
(A) Kaplan-Meier survival curve of pIpC-treated Mx1-Cre/Gemfl/fl mice (red) and control littermates (black). (B–F) Total marrow cells and complete blood counts after pIpC induction. Red circles, pIpC-treated Mx1-Cre/Gemfl/fl mice; black circles, pIpC-treated control littermates. Each circle represents a single mouse, and the line graph represents the average value at each time point. (G–J) Bone Marrow sections (G and H), blood smears (I), and spleen sections (J) from pIpC-treated Mx1-Cre/Gmnnfl/fl mice (GmnnΔ/Δ) and pIpC-treated control littermates (control). Original magnification, ×400 (G, H, and J); ×1000 (I). (K–M) Typical flow cytometry profiles of marrow cells from pIpC-treated Mx1-Cre/Gemfl/fl mice (GemΔ/Δ) and pIpC-treated control littermates (control). Quantification of data from several mice is shown in the right panels. The rectangles indicate the mean value and the error bars indicate the standard deviation of the mean. (N and O) EPO levels (N) and TPO levels (O) for control and pIpC-treated Mx1-Cre/Gemfl/fl mice. The horizontal bar indicates the median, box indicates 25th to 75th percentiles, and whiskers indicate extremes. P values calculated from Student’s t test.