Effect of purine nucleosides on cell viability of hypoxic cerebellar granule neurons: Cerebellar granule neurons from 7 day old rats were isolated and after 3 days in culture cells were exposed to normoxia and hypoxia (1% oxygen) for 6-8 hours. Prior to hypoxic insult cells were treated without purine nucleosides (Co) or with 500μM adenosine (Ado) and inosine (Ino). (A) Cells were stained with Hoechst (blue) and propidium iodide (PI, pink). (B) The percent of cell death is shown for normoxia (grey) and hypoxia (black). (C) The percent of cells protected from hypoxia by purine nucleosides is compared to the percent of cell death in controls (Co). Values represent the mean ± S.E.M., n=4. Differences were analyzed using unpaired one-tailed t-test: (B) *p<0.05 , (C) ***p<0.001. Scale bar: (A) 20 μm.