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Ann Gen Psychiatry. 2010; 9(Suppl 1): S139.
Published online 2010 April 22. doi:  10.1186/1744-859X-9-S1-S139
PMCID: PMC2991824

Evaluating the effect of aquatic extraction of Cannabis sativa seed on spatial memory consolidation in rats

Background

The existence of an endocannabinoid system in the central nervous system that consists of G protein-coupled CB1 cannabinoid receptors and endocannabinoids, including arachidonylethanolamide and 2-arachidonoylglycerol, has gained general acceptance. Recent reports suggest that this system may serve several physiological functions including learing and memory functions

Materials and methods

40 male wistar rats (3-4 month, 320-260 g) were completely divided into 4 experimental groups and control group. Cannabis sativa seed was extracted with Soxhlet apparatus. To test spatial memory, Morris water mazemaze (7 days,4 trails) was used. Experimental groups with 50 mg.kg-1, 100 mg.kg-1, 150 mg.kg -1,210 mg.kg-1 were injected in the peritoneal (IP) orderly and after one hour of injection spatial memory was done.

Results

The result show that experimental groups (50 mg.kg-1,100 mg.kg-1,150 mg.kg -1 doses), for learning time have significant level deduction in the comparison of controlgroup (p < 0.05), but experimental group with 210 mg.kg-1 dose has not significant level in the comparison of control group (p < 0.05).

Conclusions

We demonstrate tetrahydrocannabinol can change brain function as learning and memory processes and probably was done with Depolarization-Induced Suppression of excitatory (DSE) mechanism in the CA1 area of Hippocamp that with neurotransmitter regulation cause to neuroplasticity.

Acknowledgements

We thank Dr Heravi and Mr Kazemi for money support and Azad University of Mashhad for prepare Lab for Test.

References

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  • Kawamura Y, Fukaya M, Maejima T, Yoshida T, Miura E, Watanabe M, Ohno-Shosaku T, Kano M. The CB1 Cannabinoid Receptor Is the Major Cannabinoid Receptor at Excitatory Presynaptic Sites in the Hippocampus and Cerebellum. Neuroscience. 2006;26:2991–3001. doi: 10.1523/JNEUROSCI.4872-05.2006. [PubMed] [Cross Ref]
  • Kyari MZ. Extraction and Charachterization of seed oils. Int Agrophysics. 2008;22:139–142.
  • Varvel SA, Litchman AH. Evaluation of CB1 receptor knockout mice in the Morris water maze. Pharmacol Exp Ther. 2002;301:915–24. doi: 10.1124/jpet.301.3.915. [PubMed] [Cross Ref]

Articles from Annals of General Psychiatry are provided here courtesy of BioMed Central