|Home | About | Journals | Submit | Contact Us | Français|
Deoxyribonucleic acid (DNA) integrity plays a significant role in cell function. There are limited studies with regard to the role of DNA damage in bipolar affective disorder (BP). In the present study, we have assessed DNA integrity, conformation, and stability in the brain region of bipolar depression (BD) patients (n=10) compared to age-matched controls (n=8). Genomic DNA was isolated from 10 postmortem BD patients’ brain regions (frontal cortex, Pons, medulla, thalamus, cerebellum, hypothalamus, Parietal, temporal, occipital lobe, and hippocampus) and from the age-matched control subjects. DNA from the frontal cortex, pons, medulla, and thalamus showed significantly higher number of strand breaks in BD (P<0.01) compared to the age-matched controls. However, DNA from the hippocampus region was intact and did not show any strand breaks. The stability studies also indicated that the melting temperature and ethidium bromide binding pattern were altered in the DNA of BD patients’ brain regions, except in the hippocampus. The conformation studies showed B-A or secondary B-DNA conformation (instead of the normal B-DNA) in BD patients’ brain regions, with the exception of the hippocampus. The levels of redox metals such as Copper (Cu) and Iron (Fe) were significantly elevated in the brain regions of the sufferers of BD, while the Zinc (Zn) level was decreased. In the hippocampus, there was no change in the Fe or Cu levels, whereas, the Zn level was elevated. There was a clear correlation between Cu and Fe levels versus strand breaks in the brain regions of the BD. To date, as far as we are aware, this is a new comprehensive database on stability and conformations of DNA in different brain regions of patients affected with BD. The biological significance of these findings is discussed here.
Bipolar depression (BD) is one of the major psychiatric disorders characterized by recurrent depressive and manic episodes. BD affects about 1% of the population and causes severe neuropsychological impairments. The illness is implicated in functional impairment and represents an important risk factor for suicidal behavior. Twin, adoption and family studies, show that genetic factors also contribute to the etiology of this disorder. More recently, remarkable progress has been made in identifying the changes in the brain, related to the pathophysiology of BD. The neurochemical and brain imaging studies have demonstrated volume loss in the brain, in BD.[3,4] Studies have also shown a reduction in the numerical density of neurons in several brain regions including the anterior cingulate cortex and the hippocampus of subjects with BD.[5,6] The above-mentioned studies indicate that cell death plays a significant role in BD. Furthermore, the studies suggest that oxidative stress plays a role in the etiology of BD.[7–12]
The apoptotic cell death of neurons is hypothesized to have a role in neuropsychiatric disorders.[13–15] There are limited studies indicating the presence of DNA damage in BD.[11,15–19] However, DNA fragmentation has also been reported with neurodegenerative disorders.[20–23] The aim of the current study is to assess the genomic integrity in terms of DNA fragmentation, conformation, and stability in the different brain regions of BD and to ascertain whether altered genome integrity plays a role in thepathophysiology of BD.
Radiolabeled3[H]-TTP (Sp.Act.40Ci/nmol) was purchased from Amersham Radiochemicals, UK. Ribonuclease A (RNAse A), Proteinase k, Deoxyribonuclease I (DNAse I), dATP, dTTP, dCTP, dGTP, low melting agarose, DNA polymerase I (from E. coli), terminal deoxynucleotidyl transferase enzymes, 1 kb and 100 bp DNA ladders, and lamda DNA ladder were purchased from Genei, India. Ethidium bromide (EtBr), Hepes, and Tris buffers were purchased from Sigma Chemicals (USA). All other chemicals were of analytical grade and were purchased from Sisco Research Laboratories, Mumbai, India.
Eight normal and ten BD postmortem human brain samples were collected from the Depression Brain Bank of the JSS Medical College and Hospital, Mysore, India. Autopsies were performed on both sets with written informed consent obtained from the direct next of kin. The control human brains were collected from accident victims, who had no history of long-term illness, psychiatric diseases, dementia, or any neurological disease, prior to death. The BD brains were postmortem specimens and were diagnosed by using postmortem psychiatric analysis by structural clinical interviews and by referring to their medical records by psychiatrists. We found that the study subjects were not on any psychotropic medication prior to or at the time of death. In the present study, the brains were obtained from the BD suicidal victims, who died by hanging/ burns only and not by consuming poison. We have excluded subjects who had drug and alcohol abuse. The average postmortem interval between the time of death and collection of brain and freezing was ≤ six hours. The brain tissue was isolated and stored frozen at -80°C till the analysis. The clinical characteristics and agonal states of eight healthy controls and ten subjects with BD matched for age, postmortem interval (PMI), pH, sex, and freezer storage time, are provided in Table 1.
Genomic DNA was isolated from ten regions (Parietal, temporal, and occipital lobes, hippocampus, thalamus, cerebellum, hypothalamus, medulla, pons, and frontal cortex) of the frozen brain tissue by the standard ’phenol-chloroform extraction’ method of Sambrook et al., with some modifications, to prevent DNA fragmentation during isolation. The concentration of DNA was measured using the UV/ Visible spectrophotometer and noting the absorbance at 260 mm, and the purity was checked by recording the ratio of absorbance at 260 nm/ 280 nm, which should be ideally between 1.6 and 1.8.
The brain samples from BD patients and controls were matched as precisely as possible in postmortem interval, age, tissue pH, and freezing storage time [Table 1]. The representative anatomical areas had been dissected under cold conditions and transported on dry ice and stored at –80°C. The DNA was isolated from 10 different regions namely, parietal, temporal, occipital lobes, hippocampus, thalamus, cerebellum, hypothalamus, medulla, pons, and frontal cortex from eight control and ten BD brains.
The measurements of DNA fragmentation or damage were performed in 10 different brain regions of BD and age-matched control subjects by agarose gel electrophoresis. The areas like Pons, medulla, temporal lobe, thalamus, cerebellum, and frontal cortex region of the BD brains showed a higher level of DNA fragmentation when compared to the age-matched control brains [Figure 1 A–F]. However, moderate DNA damage was observed in the parietal lobe, occipital lobe, and hypothalamus of BD brains compared to controls [Figure 1 F–J]. Interestingly, the hippocampus of both BD and age-matched control brains showed no DNA fragmentation, and the DNA was intact [Figure 1H].
Single Strand Breaks are predominantly seen during DNA damage in cells. Single-stranded breakage is the end point of several types of structural insults inflicted on the genome by both endogenous and exogenous agents. Figure 2 shows the number of SSBs per microgram of genomic DNA, isolated from various brain regions. Accumulations of SSBs were more frequent in BD than in healthy controls in the following regions. Thalamus (P<0.001), pons (P<0.01), cerebellum (P<0.05), parietal lobe (P<0.02), and frontal cortex (P<0.05) accumulated significantly higher number of SSBs compared to healthy controls, from their respective regions. [Figure 2]. Interestingly, there were negligible numbers of SSBs in the hippocampus region of BD brains as well as controls.
The DNA isolated from the thalamus, frontal cortex, cerebellum, pons, and temporal lobe showed significantly higher number of DSBs than the respective controls (P<0.01) [Figure 3]. Hippocampus showed no DSBs in both the control and BD samples. Regions such as thalamus, pons, medulla, cerebellum, and parietal lobe accumulated both SSBs and DSBs, more in BD than in controls, whereas, the frontal cortex, accumulated more DSBs than SSBs. We also observed less number of DSBs in the hypothalamus DNA. These results showed that frontal cortex had more DSBs than SSBs, whereas, the thalamus, cerebellum, parietal lobe, and pons had a presence of both accumulated DSBs and SSBs. Aninteresting finding was that the hippocampus region showed the least accumulation of SSBs and DSBs in both control subjects and BD human brain samples.
Trace metal data provided new information on genomic instability in the frontal cortex, while no change was observed in the hippocampus. Redox metals like Cu and Fe were elevated in the depressive brain frontal cortex, where the SSBs were also elevated. In addition, the antioxidant Zn was reduced. These data provide direct evidence on the role of redox metal imbalance in DNA instability. This is the first finding in the literature. We did not observe DNA instability in the hippocampus. In support of this, we could find no changes in Cu, Fe, and Zn levels. This data too further confirms the role of metal imbalance in DNA instability and neuronal dysfunction [Table 2].
The tm and hyperchromicity values of genomic DNA dissolved in 0.01 M Hepes buffer, pH 7.4, is shown in Table 3. The data showed that the tm was significantly low for genomic DNA isolated from the BD brain region. The genomic DNA from the temporal lobe (P<0.001), medulla (P<0.001), frontal cortex (P<0.001), thalamus (P<0.001), pons (P<0.05), and cerebellum (P<0.05) of BD brain were significantly unstable compared to the age-matched controls. Consistent with DNA damage data, no significant difference was observed in tm for DNA isolated from the hippocampus and hypothalamus of both control and BD brains. The decrease in the tm of DNA isolated from the thalamus, temporal lobe, medulla, frontal cortex, cerebellum, and pons might be due to more strand breaks and the resulting destabilization of DNA.
The number of EtBr molecules bound per base pair (bp) of DNA is represented in Table 4. Scatchard plots of ten regions from the eight controls and ten BD brains DNA are shown in Figure 4. The EtBr binding data show that the DNA isolated from the thalamus, medulla, pons, cerebellum, temporal lobe, and frontal cortex have significantly less EtBr/ bp in BD compared to the age-matched controls (P<0.02). However, the EtBr bound/ bp to DNA from the hippocampus, occipital lobe, and hypothalamus does not differ significantly between BD and control subjects.
The CD spectra of DNA isolated from the frontal cortex, cerebellum, pons, thalamus, temporal lobe, and parietal lobe differ from their respective controls [Figure 5]. The negative peak at 200 nm was observed more in the frontal cortex, cerebellum, pons, and parietal lobe of the BD brain compared to the age-matched control subjects. The presence of a negative peak at 200 nm is the characteristic feature of secondary (altered) B-DNA. Furthermore, the positive peak at 275 nm and negative peak at 245 nm were consistently low for the DNA isolated from the temporal lobe and thalamus of the BD brain rather than for the age-matched control subjects. The CD spectra of the BD-thalamus and pons showed the blue shift compared to the controls. These results showed for the first time that genomic DNA isolated from the BD patients’ brain regions had altered conformation.
DNA conformation plays a significant role in cell physiology. The present study is focused in understanding the DNA conformation and integrity in brain regions of BD in comparison with age-matched control subjects. The changes include DNA damage in the form of SSBs and DSBs and DNA instability as indicated by the tm and EtBr binding studies. Interestingly, the changes observed appear to be brain region-specific. The DNA isolated from the thalamus, frontal cortex, medulla, cerebellum, temporal lobe, and pons are largely affected, while the parietal lobe and hypothalamus are less affected. However, the hippocampus region does not show any DNA fragmentation both in the control and BD brains. Previous MRI studies have indicated volume reductions in different regions of the BD brain compared to the controls.[3,31,33–35] The biochemical factors, namely, mitochondrial DNA-deletion andapoptosis, are associated with the etiology of BD.[9–12,36–38]
The CD studies showed that DNA extracted from the frontal cortex, cerebellum, pons, and parietal lobe of the BD depressive brain were in the B-A mixed or altered conformation. However, both control and BD hippocampus DNA were shown to be in the intact, normal, right-handed helical B-DNA conformation. It was previously shown that the genomic DNA extracted from severe Alzheimer’s disease (AD) hippocampus was predominantly in the Z-DNA conformation rather than the usual B-DNA conformation, and it was proposed that the change in conformation of DNA altered the chromatin integrity, and thus could alter DNA transactions and in turn disrupt cell functioning. It was earlier shown that DNA fragmentation reduced the high activation energy barrier required to induce the conformational and topological changes in DNA. In addition, astudy showed that there was an empiric link between late-life depression and AD, suggesting that depression may lead to the development of AD in some individuals.
Our study is new and shows that there is a selective increase of SSBs and DSBs in DNA in the thalamus, pons, frontal cortex, and medulla of BD-depressive brains compared to the age-matched controls. In contrast to a previous study that reported decreased DNA fragmentation in anterior cingulate cortex? the present study shows that the hippocampus DNA is not affected in terms of SSBs and DSBs in both control and BD-depressive brains. Consistent with the current finding, Benes et al. shows that no apoptosis is observed in the hippocampus of BD and controls. There are reports on the reduction of right hemisphere hippocampal volume in bipolar patients; some studies indicate that there is no change in the hippocampal size in BD compared to the control.[40–42] Yet, there is no uniform conclusion on the MRI changes in the hippocampus. The neurons are continuously exposed to endogenous oxidative stress, due to the brain’s high energy metabolism. The increased DNA fragmentation found in the thalamus, pons, frontal cortex, and medulla may be on account of the vulnerability to excitotoxicity? However, the intact DNA from the hippocampus may represent an adaptive compensation to oxidative stress. The genotoxic stress, the altered DNA topology, and also the insufficient DNA repair machinery may be the reason for the pathology of neurological disorders. There are limited studies in the area of genomic biology of the brain and brain disorders. Yet, the mechanisms for neuronal cell death in brain disorders are not clear. It can possibly be due to both apoptotic and necrotic cell death events. Furthermore, the functional role of genomic stability in augmenting gene expression is still not clear. The DNA repair mechanisms are much more complex and the secrets of repair enzymes need to be further explored. The clinical and biological link between neuropsychiatry and neurodegeneration is overlapping, making it difficult for scientists and for drug discovery, as also for clinicians in diagnosis. There is a lot of debate on translational research and neurochemistry in overlapping clinical conditions in neuropsychiatry–neurodegenerative diseases. The major questions are, (i) Will MRI help to design the end-points to map the cross-talk between neuropsychiatry and neurodegeneration?, (ii) Does neurochemistry mapping help to interlink clinics and neurochemistry?, (iii) Will depression be an early event and risk factor for neurodegeneration?. There are three major challenges, (i) Can neuropsychiatry illness be a risk factor for neurodegeneration?, (ii) To map neurochemical and clinical complexities, to classify neuropsychiatry versus neurodegeneration, (iii) To develop biomarker end-points to classify neuropsychiatric and neurodegenerative disorders in independent and overlapping situations. A lot of debate on the topic of understanding the translational parameters related to diagnosis and drug discovery and also understanding the complex biology in overlapping clinical conditions like the neuropsychiatry–neurodegenerative features is still intense. We propose that failure in DNA repair due to altered DNA topology may be the early onset for the pathophysiology of brain disorders. This topic needs to be investigated further.
In conclusion the present study provides new evidence of the genome integrity in terms of DNA damage, stability, and conformation to ten different brain regions of BD compared to the age-matched controls, but whether this plays a central pathogenic role in causing the disease or is a consequence of the disease needs to be further investigated.
The authors thank the Director, CFTRI, Mysore, and the Principal of the JSS Medical College, vice chancellor, JSS University, for their encouragement, and the Chairman, Molecular Biophysics Units, Indian Institute of Science, Bangalore, India for providing the facilities for Circular Dichroism Studies, as also the Department of Biotechnology, India, for the Financial Support.
Source of Support: Department of Biotechnology, India
Conflict of Interest: None declared