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A technique of bile diversion by tube choledochoureterostomy has been devised for the purpose of studying the role of bile in the intestinal absorption of drugs, This method was used in six dogs, No technical difficulties or major complications developed, as are inevitable with alternative methods, including external fistula.
There have been many attempts to exclude the bile from the intestine to study the role of bile on the absorption of food or drugs. The usual method is creation of an external fistula. However, this method has several major disadvantages. The most important of them is the difficulty in carrying the animals for a long period without complications, such as development of infection and incomplete drainage.1–3
Another approach is anastomosis of the biliary tract to the urinary tract, allowing excretion of the bile into the urine. One method used to achieve this is choledochoureterostomy, and another is cholecystonephrostomy.
Choledochoureterostomy was first described in 1913.4 In this study, a right nephrectomy was performed, followed by anastomosis of the right ureter to the common bile duct directly. Half of the animals developed a major complication, such as rupture or stricture of the anastomosis.
Cholecystonephrostomy was first described in 1924.5 In this procedure, an anastomosis is performed directly between the gallbladder and the right renal pelvis. This method appears to have fewer complications but has not achieved widespread use because the operation is technically difficult.6
Our method of tube choledochoureterostomy is easy to perform and provides uncomplicated long-term bile diversion without any technical complications.
Six female beagle dogs (9.5–12.3 kg) were used. The animals were allowed water but not food beginning the evening prior to the operation. General anesthesia was induced with intravenous sodium pentobarbiturate sodium (25 mg/kg) and maintained with oxygen, nitrous oxide, and halothane. A midline abdominal incision was made. The right kidney was freed from its bed, the pedicle was exposed, and the right renal artery, vein, and ureter were dissected free from surrounding tissue. The renal artery and vein were doubly ligated and divided. The ureter was divided at the level of ureteropelvic junction and the right kidney was removed. The bile duct was dissected and ligated at the level of the upper edge of the duodenum. A choledochostomy was made right above the ligation. One end of a 5- to 7-cm piece of polyurethane tubing (2.0 mm outside diam, 1.5 mm inside diam) was inserted through the choledochostomy toward the liver. This end of tubing was placed below the lowest hepatic branch and secured with 2-0 silk ligatures. The other end was inserted into the ureter 3 em distally and secured with 2-0 silk ligatures. The midline incision was then closed (Fig 1a and b), Postoperatively, Cephamandol 1 g im was given for 3 days, with the first dose given upon closing the wound. Serum GOT, GPT, total bilirubin, alkaline phosphatase, creatinine, and BUN were measured once a week. The urine mixed with bile was collected daily. The volume, total bilirubin, and cholesterol were measured. The animals were killed 33 days after the operation. Autopsy was performed, and cultures of the content in the bile duct and bladder were obtained.
Immediately after the operation, bile was present in the urine. The animals soon recovered from effects of operation and began eating on postoperative day (POD) 1. The stools became clay-colored and free of bile pigment. There were no surgical complications and no deaths. Postoperatively, two dogs showed a minimal increase in serum GPT and four dogs showed a minimal to mild elevation in serum alkaline phosphatase, which fell to normal after POD 7. Total bilirubin and cholesterol were present in the urine immediately after the procedure and achieved stable levels by POD 5 (Fig 2). Autopsy done on POD 33 showed patency of the bile duct, the right ureter, and the tubing. The tubing was surrounded by dense adhesions. There was no evidence of leak and no fistula between bile duct and the intestines. Cultures of the bile duct and the bladder taken at the time of autopsy were sterile.
Our method of bile diversion with tube choledochoureterostomy does not allow for direct studies of bile or bile output, but it is easy to perform and allows uncomplicated long-term survival. We have used this method for pharmacokinetic study of immunosuppressive drugs, and shown that bile was necessary for adequate absorption,7 a conclusion that has been confirmed in patients with T-tube drainage.8 There was a postoperative elevation in serum GPT and alkaline phosphatase and some variability in the excretion of total bilirubin and cholesterol in the daily collected urine. These fluctuations may have been related to temporary stenosis due to edema where the catheter was tied, but they were so minor that they did not undermine the usefulness of the method.
Supported by Research Grants from the Veterans Administration and Project Grant DK 29961 from the National Institutes of Health, Bethesda, MD.