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Surg Neurol Int. 2010; 1: 70.
Published online 2010 October 30. doi:  10.4103/2152-7806.72247
PMCID: PMC2980904

Use of sodium fluorescein in skull base tumors

Abstract

Objective:

The authors present this study using sodium fluorescein (SF) to enhance skull base tumors by performing a quantitative digital analysis of tumor enhancement. The purpose of this study is to observe the grade of SF enhancement by the tumors.

Methods:

A prospective experiment within-subjects study design was performed which included six patients with skull base lesions. Digital pictures were taken before and after the SF systemic injection, using the same light source of the microsurgical field. The pictures were analyzed by computer software which calculated the wavelength (WL) of the SF pre- and post-injection.

Results:

The group of tumors was as follows: one vestibular schwannoma, three meningiomas, one craniopharyngioma and one pituitary adenoma. The SF enhancement in all tumors was strongly positive. The digital analysis of the pictures, considering the SF WL pre- and post-injection, presented P = 0.028 (Wilcoxon T test).

Conclusions:

The enhancement of the tumors by SF was consistent and evident. The introductory results suggest the possibility of using SF as an adjuvant tool for the skull base surgery. Further studies should test the clinical application of the SF in skull base tumors.

Keywords: Cranial base tumors, fluorescence-guided surgery, sodium fluorescein

INTRODUCTION

The skull base tumors are neurosurgical challenges due to neurovascular structures involved in the treatment of these lesions. Numerous surgical techniques and equipments have been developed to access the deep anatomical sites in order to improve the outcomes of such complex lesions.

Sodium fluorescein (SF) was first used for the identification of different types of brain tumors in 1948.[20] Since then, the use of SF and others fluorescent markers has been described in the literature especially dealing with glioblastoma multiform resection.[16,18,19,32,35,37] Nevertheless, SF was not portrayed as an adjuvant for the surgical resection of skull base lesions.

The authors present this introductory experiment within-subjects study using SF in skull base tumors, by performing a quantitative digital analysis of the tumor enhancement by the substance. The application intends to observe the initial results of the skull base tumor enhancement by the SF.

METHODS

A prospective experiment within-subjects study design was performed, which included six patients with skull base lesions, who were operated between December 2008 and February 2009. The inclusion criteria were patients presenting with tumors located in the anterior, medial or posterior cranial base. The patients were informed about the transoperative experimental use of SF and written consent was obtained before the procedure.

The initial dissections were performed, and after the exposure of the tumors and their relative positioning within cranial nerves and vascular structures, a digital photo was first manually obtained using the optical lens of a microscope. The digital camera used was SONY, model DSC-W90, 8.1 megapixels, with macroactivation on and internal flash off. The light source of the pictures was the same as the microsurgical field, capturing images visualized by the surgeon at the microscope without any special filters.

A dose of 1 g of the SF 20% was injected into a peripheral vein. The second picture was taken 10 minutes after the SF injection, using the same technique described previously.

The pictures were saved in JPEG format with minimal compression and divided into two groups composed of SF pre- and post-injection images. The images were analyzed by the IMAGE PRO PLUS 4.5.1 program (Media Cybernetics, Silver Spring, MD, USA). First, the SF post-injection image was submitted for program analysis. The area of interest was defined using a rectangular frame with the tumor in central position and the surrounding neurovascular structures. Manual selection of colors was performed using level 5 of sensitivity (maximum level, range 1–5). Red color was defined to highlight the wavelength (WL) of the SF in the picture. After the area of enhancement by the SF was defined by the program, it was saved and the program calculated the total area of the picture presenting the SF WL. The absolute value obtained by such statistical analysis of the program was saved in an Excel (Microsoft, Redmond, WA, USA) spreadsheet. The SF pre-injection image of the same case was later analyzed. The same rectangular frame was applied around the tumor and neurovascular structures without SF. The specific SF WL of the post-injection picture recorded by the program was applied in the same selected area of the pre-injection picture, and the program calculated the area presenting the SF WL. The data were saved for statistical analysis in the Excel database.[12,25]

The nonparametrical Wilcoxon test was used for the statistical analysis comparing the values obtained in the two groups composed of the SF WL pre and post-injection pictures.

RESULTS

The group of six tumors was as follows: one vestibular schwannoma, three meningiomas, one craniopharyngioma and one pituitary adenoma. The meningiomas were located in the sphenoid wing, petroclival and clinoidal regions.

Table 1 presents the values of the area measured by the IMAGE PRO PLUS program, with the corresponding WL of the SF. The wide range of the simple arithmetical sum of the area of the SF WL probably occurred as a result of the variability of the light during the manual pictures at the ocular lens of the microscope. Such methodological aspect was sustained because the authors would like to capture the image in a real fashion, in order to reproduce the variability of illumination observed during the surgeries. Figures Figures11 and and22 illustrate the grade of SF enhancement observed at the surgical microscope.

Table 1
Area of SF WL measured by digital program
Figure 1
Craniopharyngioma – Intraoperative microsurgical view of the right optic nerve, internal carotid artery and their relation to the tumor. Left: pre SF injection; right: post SF injection (ON: optic nerve; ICA: internal carotid artery; TU: tumor; ...
Figure 2
Clinoid meningioma – Intraoperative photograph of the left optic nerve, olfactory nerve and their relation to the anterior clinoid meningioma. Left: pre SF injection; right: post SF injection (ON: optic nerve; TU: tumor; OLF: olfactory nerve)

In spite of a few cases, the difference between the groups pre- and post-injection of SF measured by the area with the WL of the SF in the pictures was statistically significant. The Wilcoxon T test gave P = 0.028.

Graph 1 presents the impact of the caption of the SF by the tumors measuring the SF WL pre- and post-injection.

Graph 1
Graph 1: Sodium fluorescein wave length

DISCUSSION

The impact of the SF in the brain tumor identification was first investigated by Moore et al.[20] The SF is used for ophthalmoscopic examinations and several authors tested the applicability of the SF during the surgical removal of glioblastoma multiform.[16,18,19,32,35] Other fluorescence markers have been used as important tools for improvement of glioma resections.[35]

Skull base tumors involve critical neural and vascular structures in most occasions. The surgical technique advances in cranial base surgery promote a progressive improvement of resections and prognosis of such cases.[14,911,17,21,27,33,3840] The concern about the morbidity related to dissections around the cranial nerves and arterial and venous vessels is constant during the approaches.[5,8,22,26,34] Many technological advances have been applied to improve the structure preservation, such as neuronavigational systems, intraoperative neurophysiological monitoring and neurofunctional imaging, but the effective maintenance of vascular and cranial nerve functions is defined by the possibilities of their anatomical identification during the microsurgical procedures.[14,15,17,23,24,2831] Radiosurgery is a modality of treatment of skull base tumors, which avoids the direct approach to vessels and nervous structures.[6,7,13,36]

The application of SF during cranial base surgical approaches was an extension of the previous uses of such substance. The grade of the skull base tumor enhancement by the SF was strongly positive. Even with the standard white-light microscope illumination, the enhancement of the tumors was evidenced by the yellow pigmentation after injection of the SF [Figures [Figures11 and and22].

The purpose of the study was to test the hypothesis of skull base tumor enhancement by SF at the operative field. The inclusion of four histological different tumors was carried out to develop the possible application in forthcoming studies, considering this first description. Nevertheless, the enhancement differences among different histological subtypes and the clinical impact of the SF application in the skull base lesions should be evaluated with other specific design studies.

In the present series, the use of the 1 g of SF 20% was the same as that of the original description by Moore et al.[20] The authors used such a dose due to the fact that in the former study they used the SF in different histological subtypes beyond gliomas. In gliomas series, the usual dose is 20 mg/kg.

Moore et al. described that the maximum tissue fluorescence occurred in about 2 hours after injection of the SF and the effect persisted for at least 5 hours.[20] In the skull base lesions presented, the dye was evident after 10 minutes of SF injection and persisted during tumor dissection for several hours, which is consistent with the initial observation of fluorescence in tumors located at different sites.

The dissections were performed during the time between pre- and post-injection pictures, in order to avoid prolonging surgical time. This explains some differences observed in the pictures of Figure 1.

SF staining during glioma surgery is probably related to blood brain barrier (BBB) disruption. It is discussed that BBB disruption plays the principal role for the gadolinium enhancement of the tumors on magnetic resonance imaging (MRI).[32] Tumors included in this preliminary study present marked enhancement by gadolinium on MRI and, such an aspect could be an explanation for the strong SF capture by the tumors. However, SF is a high molecular substance and in tumors with intact BBB, the dye could remain intravascular and affect tumor enhancement.

The evidence of SF in the surgical cavity helped the radical removal of gliomas since the first description.[20] The contemporary studies also reveal superior total resection results based on the same aspect.[32,35] These previous evidences point to one possible application of the SF in the cranial base neoplasms, which is the identification of some residual tumors after initial resection. In the clinoidal meningioma illustrated, Figure 2b shows that SF seems to enhance the dura around the tumor. Such data could represent dural extension of the tumor.

The use of SF is very simple. The substance presents a low cost, safe, and universally available option. The method described does not require any special microscope. In fact, it can be reproduced in any department, using a standard white-light microscope.

CONCLUSIONS

The enhancement of the tumors by the SF in the series was consistent. The highly positive effect observed in the present study allows to question about the possibility of future application of SF as an adjuvant tool for skull base surgery.

Footnotes

REFERENCES

1. Al-Mefty O. Supraorbital-pterional approach to skull base lesions. Neurosurgery. 1987;21:474–7. [PubMed]
2. Al-Mefty O. Clinoidal meningiomas. J Neurosurg. 1990;58:840–9. [PubMed]
3. Al-Mefty O, Smith R. Surgery of tumors invading the cavernous sinus. Surg Neurol. 1988;30:370–81. [PubMed]
4. Al-Mefty O, Fox JL. Petrosal approach for petroclival meningiomas. Neurosurgery. 1988;22:510–7. [PubMed]
5. Bonnal J, Thibaut A, Brotchi J, Born J. Invading meningiomas of the sphenoid ridge. J Neurosurg. 1980;53:587–99. [PubMed]
6. Chang SD, Adler JR., Jr Treatment of cranial base meningiomas with linear accelerator radiosurgery. Neurosurgery. 1997;41:1019–27. [PubMed]
7. Couldwell WT, Kan P, Liu JK, Apfelbaum RI. Decompression of cavernous sinus meningioma for preservation and improvement of cranial nerve function, Technical note. J Neurosurg. 2006;105:148–52. [PubMed]
8. Couldwell WT, Cole CD, Al-Mefty O. Patterns of skull base meningioma progression after failed radiosurgery. J Neurosurg. 2007;106:30–5. [PubMed]
9. Cusimano MD, Sekhar LN, Sen CN, Pomonis S, Wright DC, Biglan AW, et al. The results of surgery for benign tumors of the cavernous sinus. Neurosurgery. 1995;37:1–10. [PubMed]
10. de Jesus O, Sekhar LN, Parikh HK, Wright DC, Wagner DP. Long-term follow-up of patients with meningiomas involving the cavernous sinus: Recurrence, progression, and quality of life. Neurosurgery. 1996;39:915–20. [PubMed]
11. de Monte F, Smith HK, Al-Mefty O. Outcome of aggressive removal of cavernous sinus meningiomas. J Neurosurg. 1994;81:245–51. [PubMed]
12. Doncatto LF, da Silva JB, da Silva VD, Martins PD. Cutaneous viability in a rat pedicled TRAM flap model. Plast Reconstr Surg. 2007;119:1425–30. [PubMed]
13. Duma CM, Lunsford LD, Kondziolka D, Harsh GR, 4th, Flickinger JC. Stereotactic radiosurgery of cavernous sinus meningiomas as an addition or alternative to microsurgery. Neurosurgery. 1993;32:699–705. [PubMed]
14. Harris FS, Rhoton AL. Anatomy of the cavernous sinus. A microsurgical study. J Neurosurg. 1976;45:169–80. [PubMed]
15. Heth JA, Al-Mefty O. Cavernous sinus meningiomas. Neurosurg Focus. 2003;14:e3. [PubMed]
16. Kabuto M, Kubota T, Kobayashi H, Nakagawa T, Ishii H, Takeuchi H, et al. Experimental and clinical study of detection of glioma at surgery using fluorescent imaging by a surgical microscope after fluorescein administration. Neurol Res. 1997;19:9–16. [PubMed]
17. Knosp E, Perneczky A, Koos WT, Fries D, Matula C. Meningiomas of the space of the cavernous sinus. Neurosurgery. 1996;38:434–44. [PubMed]
18. Kuroiwa T, Kajimoto Y, Ohta T. Comparison between operative findings on malignant glioma by a fluorescein surgical microscopy and histological findings. Neurol Res. 1999;21:130–4. [PubMed]
19. Kuroiwa T, Kajimoto Y, Ohta T. Development of a fluorescein operative microscope for use during malignant glioma surgery: A technical note and preliminary report. Surg Neurol. 1998;50:41–9. [PubMed]
20. Moore GE, Peyton WT, French LA, Walker WW. The clinical use of fluorescein in neurosurgery. The localization of brain tumors. J Neurosurg. 1948;5:392–8. [PubMed]
21. Olivero WC, Lister JR, Elwood PW. The natural history and growth rate of asymptomatic meningiomas: A review of 60 patients. J Neurosurg. 1995;83:222–4. [PubMed]
22. O’Sullivan MG, van Loveren HR, Tew JM., Jr The surgical resectability of meningiomas of the cavernous sinus. Neurosurgery. 1997;40:238–47. [PubMed]
23. Parkinson D. A surgical approach to the cavernous portion of the carotid artery: Anatomical studies and case report. J Neurosurg. 1965;23:474–83. [PubMed]
24. Rhoton AL, Jr, Inoue T. Microsurgical approaches to the cavernous sinus. Clin Neurosurg. 1989;37:391–439. [PubMed]
25. Rodriguez AL, Stefani FS, de Oliveira Praes CE, Piaceski A, Oliveira MP, Martins P, et al. Effects of ultraviolet radiation on human cutaneous nerve fibres. Cell Prolif. 2009;42:562–7. [PubMed]
26. Sakatta K, Al-Mefty O, Yamamoto I. Venous consideration in petrosal appraoch: Microsurgical anatomy of the temporal brigding vein. Neurosurgery. 2000;47:153–61. [PubMed]
27. Samii M, Ammirati M. Cerebellopontine angle meningiomas. In: Al-Mefty O, editor. Meningiomas. 1 st ed. New York: Raven Press; 1991. pp. 503–15.
28. Samii M, Draf W, editors. 1st ed. Springer: Berlin; 1989. Surgery of the Skull Base: An Interdisciplinary Approach; pp. 386–99.
29. Samii M, Carvalho GA, Tatagiba M, Matthies C. Surgical management of meningiomas originating in Meckel’s cave. Neurosurgery. 1997;41:767–75. [PubMed]
30. Sekhar LN, Burgess J, Akin O. Anatomical study of the cavernous sinus emphasizing operative approaches and related vascular and neural reconstruction. Neurosurgery. 1987;21:806–16. [PubMed]
31. Sekhar LN, Schramm VL, Jr, Jones NF. Subtemporal-preauricular infratemporal fossa approach to large lateral and posterior cranial base neoplasms. J Neurosurg. 1987;67:488–99. [PubMed]
32. Shinoda J, Yano H, Yoshimura SI, Okumura A, Kaku Y, Iwama T, et al. Fluorescence-guided resection of glioblastoma multiforme by using high-dose fluorescein sodium Technical note. J Neurosurg. 2003;99:597–603. [PubMed]
33. Silva CE, Peron CS, Nesi A, Nunes CA, Santos SC, Silveira LC. Importance of the temporal venous drainage to the petrosal approaches of the skull base. J Bras Neurocir. 2009;20:27–32.
34. Simpson D. The recurrence of intracranial meningiomas after surgical treatment. J Neurol Neurosurg Psychiatry. 1957;20:22–39. [PMC free article] [PubMed]
35. Stummer W, Novotny A, Stepp H, Goetz C, Bise K, Heulen HJ. Fluorescence-guided resection of glioblastoma multiforme by using 5-aminolevulinic acid-induced porphyrins: A prospective study in 52 consecutive patients. J Neurosurg. 2000;93:1003–13. [PubMed]
36. Subach BR, Lunsford LD, Kondziolka D, Maitz AH, Flickinger JC. Management of petroclival meningiomas by stereotactic radiosurgery. Neurosurgery. 1998;42:437–45. [PubMed]
37. Takeda N, Tanaka R. Usefulness of intraoperative administration of fluorescein in patients with cerebral malignant gliomas. Tohoku No-Shuyou Konwakai-shi. 1991;25:96–106.
38. van Havenbergh T, Carvalho G, Tatagiba M, Plets C, Samii M. Natural history of petroclival meningiomas. Neurosurgery. 2003;52:55–64. [PubMed]
39. Wu ZB, Yu CJ, Guan SS. Posterior petrous meningiomas: 82 cases. J Neurosurg. 2005;102:284–9. [PubMed]
40. Yasargil MG, Mortara RW, Curcic M. Meningiomas of the basal posterior cranial fossa. Adv Tech Stand Neurosurg. 1980;7:1–115.

Articles from Surgical Neurology International are provided here courtesy of Medknow Publications