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Treponema pallidum rapidly disseminates from a genital site of inoculation to diverse organs where it establishes persistent infection. T. pallidum has long been regarded as a stealth pathogen because of its poorly antigenic and non-inflammatory surface. There is now increasing evidence that antigenic variation also contributes to the ability of the spirochete to evade host defences. Among the small number of proteins encoded by the T. pallidum genome with sequence similarity to well characterized transcription factors is TP0262, an orthologue for cAMP regulatory protein (CRP) of E. coli. Giacani and co-workers identified sequences matching the CRP consensus-binding motif upstream of the promoters of tprE, tprG, and tprJ, three members of the T. pallidum repeat (tpr) gene family (Subfamily II). Using EMSA, DNaseI footprinting, and an E. coli-based reporter system, they demonstrated that TP0262 specifically recognizes the putative binding sequences and that DNA binding is cAMP-dependent. Their report, a major methodological advance for syphilis research, suggests that T. pallidum has appropriated a paradigmatic global regulator of metabolic processes in heterotrophic bacteria to further its capacity for immune evasion in its obligate human host.
Treponema pallidum, the causative agent of syphilis, is a sexually transmitted spirochaete that rapidly disseminates from a site of inoculation in the genital region to diverse organs where it can establish persistent, even lifelong, infection (Lukehart, 2004). The remarkable ability of this extracellular bacterium to evade host clearance mechanisms has earned it the designation “stealth pathogen” (Radolf et al., 2006). Efforts pre-dating the genomic era to uncover the basis for T. pallidum stealth pathogenicity have focused on the unusual molecular architecture of its outer membrane, a lipid bilayer with a paucity of integral-membrane proteins that is non-inflammatory and presents few surface antigenic targets to the host immune system (Radolf, 1995). Since the publication of the T. pallidum genomic sequence (Fraser et al., 1998), evidence has mounted that a protein-deficient outer membrane is only one facet of the persistence strategy of this spirochaete. T. pallidum may possess a complex system for antigenic variation to further confound host antibody responses (Centurion-Lara, 2006;LaFond and Lukehart, 2006). T. pallidum also must acquire nutrients in every microenvironment in which it takes up residence and, like all bacteria, must be able to sense and respond to changes in nutrient availability as well as other environmental stresses (Hazlett et al., 2002; Desrosiers et al., 2007). Delineating virulence-related and physiologic processes, their interrelationships, and the regulatory networks that orchestrate them at transcriptional and post-transcriptional levels is a daunting challenge with a bacterium that can neither be cultivated nor manipulated genetically. The report by Giacani et al. (Giacani et al., 2009) in this issue of Molecular Microbiology represents a step along the arduous path of developing the surrogate systems needed to elucidate gene regulation in T. pallidum. Their finding that T. pallidum might have appropriated CRP (cAMP regulatory protein), the paradigmatic global transcriptional regulator of metabolism in Gram-negative bacteria, for the purpose of immune evasion represents an unanticipated convergence of ostensibly unrelated themes in syphilis pathogenesis research.
When more than one carbohydrate is present in the growth medium, free-living heterotrophic bacteria will typically utilize a preferred carbon source while repressing uptake of less desirable, alternative substrates until the primary source is depleted. The regulatory processes by which bacteria prevent utilization of secondary carbon sources, collectively called carbon catabolite repression (CCR), have been intensively studied in Escherichia coli and Bacillus subtilis (Fig. 1)(Gorke and Stulke, 2008; Warner and Lolkema, 2003). In both model organisms, CCR centres about the multi-component phosphoenolpyruvate: sugar phosphotransferase (PTS) system for uptake and phosphorylation of sugars but employs markedly dissimilar mechanisms to achieve similar regulatory outcomes. In E. coli, the ratios of phosphoenolpyruvate to pyruvate, reflective of glucose consumption in glycolysis, determine the phosphorylation state of the EIIA component of the PTS. EIIA is preferentially dephosphorylated when E. coli cells grow in the presence of phosphate-draining, rapidly metabolizable sugars (e.g., glucose); unphosphorylated EIIA allosterically inhibits non-PTS sugar permeases. Phosphorylation of EIIA, which predominates when glucose is unavailable, activates membrane-bound adenylate cyclase. At sufficiently high levels of cAMP, CRP becomes activated and binds to specific DNA sequences at or near the promoters of genes encoding transporters and enzymes required for utilization of alternative carbon sources. In B. subtilis, histidine protein (Hpr) and Hpr kinase, (HprK), rather than EIIA, are the key regulators. HprK is activated by glycolytic intermediates, such as fructose-1–6-P, whereupon, at the expense of ATP, whereupon it phosphorylates Ser46 in the “activation site” of HPr. HPr (Ser-P) complexes with CcpA (catabolite control protein A), which then binds to cre sites of catabolic genes, repressing their transcription. As in E. coli, phosphorylation of HPr at the “catalytic site”, His15, by EI (PtsI) of the PTS system promotes phosphorylation of EIIA, stimulating PTS-mediated transport and phosphorylation of glucose. In B. subtilis, Hpr(His-P) also negatively regulates both glycerol kinase (GlpK) and transcriptional regulators of alternative source PTS permeases. An important distinction between the two schemes is that regulation in E. coli is linked directly to PTS function via EIIA, whereas in B. subtilis, the linkage is indirect (Fig. 1). It is theoretically possible, therefore, for HPrK/Hpr to regulate metabolic processes independent of the PTS system. Because pathogenicity programs in bacteria are often tied to the accessibility of specific nutrients associated with a particular host niche, it should not be surprising that there are now numerous examples in which CCR-mediated responses are involved in the coordination of virulence expression (Gorke and Stulke, 2008).
Consistent with its enigmatic evolutionary route (Norris and Weinstock, 2006), T. pallidum harbours an odd assortment of CCR-related molecules that does not conform to either the Gram-negative or Gram-positive paradigms. It contains Hpr (TP0589) and HprK (TP0591), as well as a pseudogene encoding a nonfunctional EI (TP0575), but does not have PTS permeases (Gonzalez et al., 2005). In an elegant biochemical analysis using purified recombinant T. denticola (Td) Hpr and HprK orthologues, Gonzalez and colleagues (Gonzalez et al., 2005) showed that Td HprK is capable of phosphorylating HpR (presumably at Ser46) and is activated by 1–6 fructose bis-phosphate and gluconate-6-P. They speculated that in both T. pallidum and T. denticola, Hpr/HprK exerts a regulatory function divorced from PTS sugar transport and phosphorylation. Curiously absent from this signalling mechanism, however, is a downstream regulatory target, because both treponemes lack CcpA. The presence in T. pallidum of a well-developed cAMP signalling system without PTS permeases deepens the conundrum. Surprisingly, the spirochaete contains not only adenylate cyclase (TP0485) but also four proteins (TP0089, TP0261, TP0262, and TP0995) with cAMP binding domains (Subramanian et al., 2000;Giacani et al., 2009). Of the latter, only TP0262 has extensive sequence homology with E. coli CRP (Fig. 2A). Homology modelling confirms that TP0262 and CRP are close structural orthologues (Fig. 2B). Giacani et al. (Giacani et al., 2009) took note of the high degree of similarity between CRP and TP0262 and used it as the starting point for their ground breaking study (see below).
Although glucose is the primary energy source for T. pallidum, it also contains ABC transporters thought to be collectively capable of importing multiple sugars and, in an in vitro tissue culture system, this bacterium can metabolize mannose and maltose in addition to glucose (Gonzalez et al., 2005) (Fraser et al., 1998;Cox and Radolf, 2006). These data imply that, under some circumstances, T. pallidum can turn to alternative substrates to generate ATP and that, conversely, when glucose is readily available, it would have need for some form of CCR to modulate import of less desirable substrates. From the above, one would expect HprK/Hpr, rather than TP0262, to be the primary regulator of this putative CCR mechanism. Liberated from its conventional physiologic role, TP0262 might then have evolved to coordinate responses of more functionally divergent target genes and also to engage in cross-talk with other members of the treponemal cyclic nucleotide signalling family. Although many features of these novel signal transduction pathways and their potential points of intersection are difficult to discern, one might surmise that they collectively endow the spirochaete with a capacity for sensing and responding to environmental cues that could come into play at different sites and stages of syphilitic infection.
Given the dearth of recognizable transcription factors in T. pallidum (Fraser et al., 1998;Subramanian et al., 2000), TP0262 is an attractive starting point for an examination of global gene regulation in T. pallidum despite or, as reasoned by Giacani and co-workers (Giacani et al., 2009), because of its possible “orphan” status as a metabolic regulator. They began by using the powerful tool of the modern molecular biologist, bioinformatics, to scan the T. pallidum genome for matches to the E. coli CRP consensus binding motif. Of the more than 200 hits obtained (but, unfortunately, not presented in tabular form), the ones of immediate interest were upstream of tprE, tprG, and tprJ, subfamily II in the 12 member tpr (T. pallidum repeat) gene family. The Tpr protein family has come under intense scrutiny since its discovery a decade ago by this same group (Centurion-Lara et al., 1999) and others (Fraser et al., 1998). Tprs are candidate rare outer membrane proteins that, by loose analogy with the systems for antigenic variation in the relapsing fever and Lyme disease spirochaetes (Norris, 2006), have been proposed to endow T. pallidum with the ability to alter its surface antigenicity (LaFond and Lukehart, 2006). Subfamily II genes have drawn interest of late because they are variably expressed in different T. pallidum clinical isolates and have hypervariable homopolymeric guanosine tracts near their transcriptional start sites that may be involved in their transcriptional control (Giacani et al., 2007). The in silico results were confirmed in electrophoretic mobility shift assay (EMSA) and DNaseI footprinting using purified recombinant TP0262. Using a recently devised E. coli-based reporter system (Giacani et al., 2007), expression of TP0262 was shown to modulate the promoter activities of the three genes. Lastly, in both EMSA and E. coli, the DNA-binding activity of TP0262 was cAMP-dependent. Based on prior mapping of transcriptional start sites (Giacani et al., 2005), the authors propose that CRP modulates transcription of the three tpr genes, in concert with the polyG tracts, via interactions with σ70. It is interesting to note, however, that the binding sites for all three genes are considerably further upstream from their respective promoter elements than are the sites for canonical CRP-dependent promoters (Busby and Ebright, 1999), though within the extended range observed in genome-wide surveys of CRP-dependent genes (Zheng et al., 2004;Gosset et al., 2004). Resolution of this potential disparity will require a more detailed comparative analysis of CRP and TP0262 DNA-binding interactions. The authors candidly acknowledge the major limitation of their study: presently, methodology is not available to corroborate the results in T. pallidum. On the horizon, however, are technologies (e.g., chromatin immuoprecipitation) that promise to circumvent the refractoriness of spirochaetes to in vitro cultivation and genetic manipulation.
Clearly, our understanding of how T. pallidum manages its transcriptome is lagging far behind that of most other major bacterial pathogens. One of the most important outcomes of the present study is that it will help put to rest the pre-genomic view of the syphilis spirochaete as a transcriptionally invariant organism. For further evidence of the fierce and protracted duel between pathogen and host that ensues following inoculation, one need only look to the fact that T. pallidum encodes seven different sigma factors, as many as E. coli, and four more than the Lyme disease spirochaete, Borrelia burgdorferi, which must accommodate to both arthropod and mammalian milieus (Table 1). As Giacani and co-workers realize, TP0262 has a rich field in which to function and network. Both noteworthy and perplexing is that the T. pallidum repertoire consists of a mélange of E. coli- and B. subilis-like sigma factors, including housekeeping (σA and σ70) and stress-related sigma factors (σ24 and σB) from both lineages (Subramanian et al., 2000;Fraser et al., 1998;Fraser et al., 1997;Hecker et al., 2007;Raivio and Silhavy, 2001). T. pallidum also lacks RpoS, a general stress response regulator in E. coli (Hengge-Aronis, 2002) and a principal global regulator of differential gene expression during tick-to-mammal transmission of B. burgdorferi (Caimano et al., 2007;Hubner et al., 2001). Syphilologists are learning not to equate the biosynthetic limitations of T. pallidum with transcriptional and regulatory simplicity. From a pathogenesis standpoint, this emerging picture of a wily, adaptive adversary makes sense. The complex natural history of syphilis makes plain that the parasitic strategy of the spirochaete is ambitious. It is hard to imagine that the bacterium could implement such an agenda without invoking complex and versatile genetic programs.