|Home | About | Journals | Submit | Contact Us | Français|
Children with congenital heart disease (CHD) who survive surgery often present impaired neurodevelopment and qualitative brain anomalies. However, the impact of CHD on total or regional brain volumes only received little attention. We address this question in a sample of patients with 22q11.2 deletion syndrome (22q11DS), a neurogenetic condition frequently associated with CHD. Sixty-one children, adolescents, and young adults with confirmed 22q11.2 deletion were included, as well as 80 healthy participants matched for age and gender. Subsequent subdivision of the patients group according to CHD yielded a subgroup of 27 patients with normal cardiac status and a subgroup of 26 patients who underwent cardiac surgery during their first years of life (eight patients with unclear status were excluded). Regional cortical volumes were extracted using an automated method and the association between regional cortical volumes, and CHD was examined within a three-condition fixed factor. Robust protection against type I error used Bonferroni correction. Smaller total cerebral volumes were observed in patients with CHD compared to both patients without CHD and controls. The pattern of bilateral regional reductions associated with CHD encompassed the superior parietal region, the precuneus, the fusiform gyrus, and the anterior cingulate cortex. Within patients, a significant reduction in the left parahippocampal, the right middle temporal, and the left superior frontal gyri was associated with CHD. The present results of global and regional volumetric reductions suggest a role for disturbed hemodynamic in the pathophysiology of brain alterations in patients with neurodevelopmental disease and cardiac malformations.
Congenital heart diseases (CHD) are among the most frequent birth defects. Over the past few decades, progress in surgical techniques has allowed for the correction of most CHD, so that an increasing number of children and adults live with surgically corrected CHD (Wernovsky et al. 2005). Nevertheless, CHD and its correction still have neurological and developmental costs (Wray 2006). Survivors often present difficulties in expressive language, visuomotor skills, and fine motor function (Bellinger et al. 1999), as well as inattention and hyperactivity (Shillingford et al. 2008). Non-specific structural brain anomalies associated with these impairments have been reported, which include microcephaly, abnormalities of the cortical mantle (Glauser et al. 1990), and frequent periventricular leukomalacia (Gaynor 2004). The pathophysiology of brain anomalies associated with CHD has largely incriminated intraoperative support mechanisms, leading to continuous improvement of surgical procedures (Nelson et al. 2008). But more recently, it has also been evidenced that neurological abnormalities, such as microcephaly or leukomalacia, may be present before surgery (Limperopoulos et al. 1999; Mahle et al. 2002; Miller et al. 2007). As reduced cerebral blood flow has been shown in fetuses (Donofrio et al. 2003) and neonates with complex CHD (Licht et al. 2004), presurgical brain abnormalities may be the consequence of decreased perfusion pressure in the developing brain. Despite increasing evidence that reduced blood perfusion impairs brain growth in individuals with CHD, the quantification of cerebral volumes in children or adult survivors only received little attention. Further, the question as to how cerebral volume may be altered regionally in survivors remained similarly unaddressed.
If we look at brain anomalies related to CHD from another point of view, we cannot ignore the numerous neurogenetic conditions frequently associated with CHD in which abnormalities of the brain structure are well documented, such as Williams syndrome, Down syndrome, or 22q11.2 deletion syndrome (22q11DS; Meyer-Lindenberg et al. 2006; Schaer and Eliez 2007; Teipel and Hampel 2006; Gothelf et al. 2008; Martens et al. 2008). Among neurogenetic syndromes, the one with the strongest association with CHD is probably 22q11DS. Prospective studies of neonates with CHD have shown that deletion at 22q11.2 accounts for one third of patients with conotruncal heart defect (most often tetralogy of Fallot and truncus arteriosus) and one half of patients with interrupted aortic arch (Hay 2007). Moreover, 50% to 75% of patients with 22q11DS have CHD (McDonald-McGinn et al. 1999; Ryan et al. 1997). In addition, to a strong bidirectional association between CHD and 22q11DS, striking similarities in the difficulties presented by children affected with 22q11DS and children with CHD have led to the suggestion that part of the cognitive or cerebral phenotype in 22q11DS may be caused by CHD (Wernovsky et al. 2005). Specifically, children with 22q11DS show visuospatial impairments, fine motor difficulties, executive dysfunction, and frequent attention problems (Gerdes et al. 1999; Swillen et al. 1999), all of which are commonly seen in non-syndromic patients with CHD. Although patients with 22q11DS and CHD demonstrate more severe intellectual impairments than patients with CHD alone (Atallah et al. 2007), comparing children with 22q11DS but with and without CHD offers an interesting framework to study the effect of CHD on brain structure and cognition. To date, no study has observed any differences in the cognitive outcome of children with 22q11DS with and without CHD (Gerdes et al. 1999; Simon et al. 2002; Bearden et al. 2001; Swillen et al. 1997; De Smedt et al. 2007). Similarly, previous neuroimaging studies grouping participants with widely varying CHD did not evidence any significant difference in brain morphology (Bearden et al. 2007; Bingham et al. 1997; Kates et al. 2001). However, using cardiac surgery as a strict clinical criteria, we previously observed a significantly reduced degree of cortical folding associated with CHD in patients with 22q11DS (Schaer et al. 2009), which suggests that the most severe cardiac anomalies affect early brain development in 22q11DS. In the present study, we address the question of regional volumetric reductions associated with CHD using a recent method that measure gyral volumes across the brain (Desikan et al. 2006). First, we quantify volumetric reductions that are specific to the syndrome by comparing patients to controls. Then, we identify the regions that are specifically altered in patients with surgically corrected CHD compared to patients without CHD.
Sixty-one individuals with 22q11.2 deletion syndrome participated in the current study. The sample consisted of children, adolescents, and young adults, with an average age of 15.6±8.9 (36 females, 25 males). Patients had an average IQ of 68.7±12.0. Participants were recruited through announcements to regional parent associations. All individuals were native French- or English-speaking individuals and were assessed in their native language. Only children with a confirmed 22q11.2 deletion were included in the study. Aside from the large prevalence of cardiac malformations presented below, the 61 patients also presented a variety of medical comorbidities known to be commonly associated with the syndrome. Facial dysmorphic features and palate or pharyngeal malformations were highly prevalent (including 25 patients requiring corrective surgery); a large proportion of patients suffered from endocrine disorders including hypoparathyroidism or repeated infections due to thymic hypoplasia; three patients had unilateral renal agenesis. At the psychiatric level, 19 of the 61 patients presented hallucinations (nine females/ten males, average age 22.5±7.5), 17 delusions (eight females/nine males, average age 22.0±9.4), and five met the DSM-IV criteria for schizophrenia (two females/three males, average age 31.2±4.5). At the time of their participation, eight patients were taking psychotropic medications (four females/four males, average age 27.7±8.2) and three were taking antiepileptic medications (two females/one male, average age 23.1±11.3).
A history of cardiac surgery was documented using a questionnaire to the parents. In addition, medical reports, including ultrasound examinations and surgical reports, were examined. Twenty-seven patients with major cardiac defects were identified and constituted the CHD subgroup (17 females/ten males, mean age 13.7±8.3). The type of CHD and the age at cardiac surgery are detailed for each patient in Table 1. Eight patients were excluded at analysis for unclear cardiac status (two with retroesophageal subclavian artery, six with documented interventricular communication with spontaneous closure at follow-up). Twenty-six patients with normal heart, or with benign heart murmurs at birth with normal ultrasound examination, were included in the noCHD subgroup (13 females/13 males, mean age 17.6±9.8). Although the age difference between the CHD and noCHD subgroups did not reach significance (p=0.121), age was covaried out in all statistical analyses.
IQ scores in the subgroup of patients with CHD (66.7±12.6) did not differ from IQ in the subgroup without (70.0±11.6, p=0.333), neither did PIQ (p=0.365) nor VIQ (p=0.364). In further exploratory statistics, we subdivided all patients who we included in the CHD analyses (n=53) into two subgroups of equal size according to their IQ performances. The low performing subgroup (FSIQ ≤ 67) was composed of 26 patients with an average IQ of 58.2±6.7 (mean age 17.3±9.5), and the high performing subgroup (FSIQ ≥ 68) was composed of 27 patients with an average IQ of 78.1±7.0 (mean age 14.1±8.8). The proportion of low-performing patients in the CHD subgroup (17 out of 27, 65.4%) was significantly higher than the proportion of low-performing patients in the no-CHD subgroup (nine out of 26, 34.6%, p=0.039, ϕ=0.283). This difference appeared mainly driven by the adolescents and adults with CHD, as the nine patients with CHD who were older than 14 years old were all low performers.
Among the patients with hallucinations, the proportion of patients with CHD (six out of 27) did not significantly differ from the proportion of patients without (nine out of 26, p=0.372, ϕ=−0.138; four patients with hallucinations were not included in the cardiac analyses because of their unclear cardiac status). The proportion of patients with CHD and delusions (five out of 27) did not significantly differ from the proportion of patients with delusions and without CHD (11 out of 26, p=0.077, ϕ=−0.259; one patient with delusion and unclear cardiac status). Finally, the proportion of patients with schizophrenia and CHD (two out of 27) did not significantly differ from the proportion of patients with schizophrenia and without CHD (two out of 26, p=1.0, ϕ=−0.005).
The comparison group was comprised of 80 typically developing individuals (44 females, 36 males). The control group had an average age of 15.9±8.4 and an average IQ of 111.7±12. Individuals were recruited through a newsletter distributed at public schools and in the Geneva community. A complete medical history, as well as parent report (CBCL in children and adolescents; Achenbach 1991) and self-report (SCL-90R in adolescents and adults; Derogatis 1983) behavioral questionnaires were used to screen control participants. Subjects with a history of past or present neurological or psychiatric disorders were excluded. Written informed consent was received from all subjects, as well as the parents of subjects younger than 18 years of age, in accordance with protocols approved by the Institutional Review Board of Geneva University School of Medicine.
Cerebral magnetic resonance images were acquired with a T1-weighted 3D volumetric pulse sequence using a Philips Intera 1.5-T scanner as a series of 124 contiguous coronal slices, with a voxel size of 0.9375×0.9375×1.5 mm (TR = 35 ms, TE = 6 ms, flip angle = 45°, NEX = 1).
Cortical reconstruction and volumetric segmentation were performed using published algorithms included in FreeSurfer software (Harvard University; Dale et al. 1999; Fischl et al. 2001). Briefly, processing consisted of removal of non-brain tissue (Sgonne et al. 2004), automatic segmentation of the subcortical gray matter structures (Fischl et al. 2002), and the extraction of cortical surfaces, which was performed according to previously published protocols (Dale et al. 1999; Fischl and Dale 2000). Both intensity and continuity information from the entire three-dimensional MR volume are used in segmentation and deformation procedures, thus producing accurate representation of cortical thickness or volumes. These procedures have been validated against histological studies (Rosas et al. 2002), manual measurements (Kuperberg et al. 2003; Salat et al. 2004), and shown reliable across scanner manufacturers and field strengths (Han et al. 2006).
Subsequent to cortical reconstruction, the cortex is subdivided into units based on gyral and sulcal structures (Desikan et al. 2006). This parcellation method based on major sulci has been shown to be both valid and reliable, with high intraclass correlation coefficient between the manual and automated procedures for both cortical volume estimates and parcel boundaries. The parcellation produces 34 gyral regions subdivided into 11 frontal regions, nine temporal regions, five parietal regions, four occipital regions, four parts of the cingulate cortex, and finally the corpus callosum which is designed to improve the reliability of the placement of the other parcels (for details, please refer to the validation article; Desikan et al. 2006). In the present study, the frontal pole and the banks of the superior temporal sulcus regions that exhibited relatively poor reliability in the validation article were excluded from statistical analyses. Cortical volume was therefore estimated for 31 parcels in each hemisphere for each subject.
ANOVA was used to compare total cerebral volume between groups. Subsequent MANCOVA compared the 31 ROIs in each hemisphere with diagnosis as the fixed factor and both age and total cerebral volume as covariates. Results are reported both with a statistical threshold of p<0.05 and with a Bonferroni’s corrected threshold of p<0.0016.
In order to test whether volumetric alterations were associated with CHD, we then conducted the same MANCOVA on the 31 parcels in each hemisphere, using age and total cerebral volume, but with three conditions as our fixed factor (control, patient with CHD, patient without CHD). IQ was not included as a covariate in the model, as it was not a significant predictor of the model (left: p=0.955; right: p=0.545). Parcels that were significant at p<0.05 were further tested in pairs using a Scheffe post hoc on cortical volumes residualized for age. Post hoc results are also reported at p<0.05 and p<0.0016.
In order to assess the specificity of cardiac malformations or surgery against other type of malformations or surgery, we also compared total and regional cerebral volumes between the 25 patients who underwent palate or pharyngeal surgery (14 females/11 males, average age 17.0±9.6) and the 36 patients without (22 females/14 males, average age 14.7±8.4). Of note, the presence of severe palate or pharyngeal malformations was not associated with the presence of CHD in our sample of patients (11 of the 16 patients without CHD and ten of the 27 patients with CHD underwent palate or pharyngeal surgery).
A 12.4% reduction in cerebral volume was observed in patients with 22q11DS compared to controls (see Table 2), the regional distribution of which is further depicted in Fig. 1. Specifically, Fig. 1 demonstrated that cortical reductions seen in 22q11DS were the largest in the posterior and medial parts of the brain.
Detailed statistical significance reported in Table 2 confirms the pattern of cortical alteration visible in Fig. 1. Significantly preserved (i.e., relatively increased) cortical regions in the syndrome are exclusively located in the frontal lobes. Posterior regions with the greatest reduction in 22q11DS reach almost equal significance in both hemispheres, at the precuneus, fusiform gyrus, and the most anterior part of the cingulate cortex. Widespread reductions are also observed in the parietal and occipital lobes, although these did not reach significance after correcting for the number of tests.
We observed a prominent association between congenital heart disease and reduced total and regional cerebral volumes. While patients with 22q11DS and normal cardiac status showed a 6.9% reduction in total cerebral volume compared to controls, patients who underwent cardiac surgery had an average of 16.9% reduction. Statistical significance in Table 3 demonstrates two patterns of cortical changes in patients with 22q11DS and CHD, highlighted with different colors on Fig. 2. First, in yellow regions on Fig. 2, patients without CHD show an intermediate level of reduction between the typically developing subjects and the most affected patients with CHD. Second, 22q11DS patients with normal cardiac status showed completely normal volumes in three cortical regions that were exclusively reduced in CHD. These regions are highlighted in red on Fig. 2 and consist of the right middle temporal, the left superior frontal, and the left parahippocampal gyri.
In further exploratory statistics, we conducted correlational analyses between age at cardiac surgery and cerebral volumes. Though, age at surgery was not a significant predictor of total cerebral volume, neither in the whole subgroup of patients with CHD (Pearson’s correlation coefficient =−0.014, p=0.944) nor after excluding the outlier patient who was operated at 11 years old (Pearson’s correlation coefficient = 0.203, p=0.331). Similarly, no significant effect of age at surgery was observed on regional cortical volumes in the 31 parcels (after excluding the outlier, right hemisphere: p=0.792, left hemisphere: p=0.492).
Finally, using palate or pharyngeal surgery as the fixed factor, we did not evidence any difference in total (p=0.731) or regional cerebral volumes (right hemisphere: p=0.771, left hemisphere: p=0.737).
In the present study, we used an accurate method for measuring regional volumes over the cortex in 22q11DS with the specific aim of quantifying the effect of CHD on brain development in the syndrome. In the entire cohort of patients with 22q11DS, independent of their cardiac status, we were able to replicate previous volumetric results that used other methods (Eliez et al. 2000; Kates et al. 2001; Simon et al. 2005; Kates et al. 2004; Campbell et al. 2006). In summary, the 12.4% reduction of the cerebrum observed here is in accordance with previous measurements (Eliez et al. 2000; Kates et al. 2001). Further, thanks to the increased precision allowed by maps of continuous volumetric changes over the cortex, we were able to confirm the rostrocaudal gradient in brain alterations that has been previously suggested using techniques with poorer spatial resolution. Indeed, as noted by Gothelf et al. (2008), a more important reduction of caudal structures was evidenced at the lobar level (Eliez et al. 2000; Kates et al. 2001; Simon et al. 2005), as well as in specific regions of interests such as basal ganglia (Kates et al. 2004; Campbell et al. 2006; Eliez et al. 2002), the thalamus (Bish et al. 2004), the corpus callosum (Machado et al. 2007), and the fusiform gyrus (Glaser et al. 2007). Turning to potential implications of the rostrocaudal gradient of brain alterations, several authors have pointed out that frontal preservation may help to protect verbal performances in children with 22q11DS, while posterior reduction may alter arithmetic and visuospatial skills (Eliez et al. 2000; Simon et al. 2005; Kates et al. 2004; Campbell et al. 2006).
In addition to a rostrocaudal gradient, the precision brought by the continuous measurement over the three-dimensional cortical surface also demonstrates a previously unreported lateromedial gradient of brain alterations in 22q11DS. As a result of the rostrocaudal and lateromedial gradients, we observe that the regional distribution of cortical reductions on Fig. 2 is proximal to the territory supplied by the posterior cerebral artery. The posterior cerebral artery supplies the brain stem, cerebellum, caudal part of the thalamus, and striatum and the uncus, fusiform gyrus, inferior temporal gyrus, pericalcarine region, cuneus, lingual gyrus, precuneus, and the occipital pole at the cortical level (see also Fig. 3, adapted from an article by van der Zwan and Hillen 1991). Given that various vascular anomalies are commonly associated with 22q11DS, Shprintzen (2000) has suggested that abnormal brain development in the syndrome may be caused by reduced perfusion related to cerebrovascular malformations. However, we were missing empirical support for this hypothesis. Only one study reported cerebral angiography in the syndrome (Chow et al. 1999), observing up to 55% of minor vascular anomalies. Despite this angiographic study included a small number of subjects, Chow and colleagues reported an increased incidence of hypoplasia of the (right) posterior cerebral artery. Based on Shprintzen’s original hypothesis and on the angiographic observations by Chow et al., we speculate that the cortical reductions observed here point to reduced blood flow in the territory of the posterior cerebral artery, possibly altering cerebral growth in these regions. The observation that non-cortical regions supplied by this artery are also reduced corroborates this hypothesis (namely the brain stem (Eliez et al. 2001), cerebellum (Eliez et al. 2001; Bish et al. 2006), caudal part of the thalamus (Bish et al. 2004), and caudal part of the corpus callosum (Machado et al. 2007; Antshel et al. 2005)). The potential implications of reduced blood supply over the territory of the posterior cerebral artery opens avenues for more detailed exploration of cerebrovascular anomalies in the syndrome.
A vascular hypothesis of cerebral alterations in the syndrome is even more likely given the regional distribution of reduced cortical volumes in the group of patients with CHD. Among regions exclusively affected in presence of congenital heart disease are the (left) superior frontal gyrus and the (right) middle temporal gyrus. Again, these differences may be analyzed in light of the arterial supply territories. As depicted in Fig. 3, variability in arterial territories is relatively high. However, the superior frontal and the middle temporal gyri are the only two gyri for which their entire length is located at the junction between two arterial territories (namely between the anterior and middle cerebral arteries for the middle frontal gyrus, and between the anterior and the posterior arteries for the middle temporal gyrus). Decades of neurological observations have taught us that these regions, known as watershed areas, are the first to suffer with reduced perfusion pressure in the brain (Liu et al. 2001; Chao et al. 2006). We can thus speculate that hypoperfusion related to CHD has particularly impaired brain growth in the superior frontal and middle temporal gyri.
Finally, we also observed a specific reduction of the left parahippocampal region associated with CHD. While a direct association between this region and arterial territories is less straightforward, this finding becomes interesting in light of the known vulnerability of this region to a variety of stressful events. Indeed, the hippocampus (a non-cortical region which was not measured in the present study, but which is known to be reduced in the syndrome: Debbané et al. 2006; Deboer et al. 2007; Kates et al. 2006) is a structure known to be highly sensitive to anoxia (Di Paola et al. 2008; Jourdain et al. 2002) or to diverse kind of stress (Bremner et al. 1995; McEwen 1999; Sapolsky 2000). It is possible that the nearby parahippocampal region is similarly sensitive to stress related to CHD or to surgery on the post-natal developing brain.
We argue that the absence of cerebral repercussions of the other core malformations associated with the syndrome, namely palate or pharyngeal malformations, as well as the above-mentioned reasons, both support a specific role for hemodynamic factors in reduced cortical volumes in patients with CHD. Though, alternative explanations also need to be considered. Indeed, the association between CHD and the observed cerebral anomalies may be a co-occurrence rather than a causative effect. Common embryologic origins of the malformed heart and brain could be incriminated. For instance, abnormal migration of cells of the neural crest is thought to be partially responsible for heart anomalies in 22q11DS (Scambler 2000) and may also be a good candidate for certain brain alterations.
The present study opens avenues for furthering our understanding of the pathophysiology of brain alterations in 22q11DS and related to CHD. However, the establishment of a clear relationship between hemodynamic status and the observed volumetric reductions remains impaired by some limitations. First, cardiac defects are largely incomparable in terms of hemodynamic disturbance, and unfortunately, the present sample size does not allow extensive explorations of the effect of specific cardiac malformations on brain structure. Second, information about cardiac status was obtained retrospectively from cardiologists, at the time of the patients evaluation in our research program. In the present sample, surgical procedures were performed in different hospitals in Switzerland or in France between 1971 and 1996. As a result, measures which would have been interesting to correlate with brain volumes, such as for instance oxymetry, were not available. Finally, due to the lack of angiographic data, we were not able to confirm a clear association between cerebrovascular malformations and cerebral reductions. Accordingly, recommendations for future studies in the syndrome include (1) a precise data collection about cardiac history in all patients with 22q11DS and (2), if possible, the acquisition of angiographic exams (e.g., magnetic resonance angiography) jointly with other structural MRI acquisitions.
Whatever the cause of the additional volumetric alterations in patients with CHD, the findings of the current study point to potential cognitive repercussions. For instance, we observed that a significantly larger proportion of low performing patients were found in the CHD than in the noCHD subgroups. We also observed that none of the adults with 22q11DS who underwent cardiac surgery were included in the high-performing subgroup of patients. If we expected stronger cognitive differences, there may be several explanations for the absence of a clearer association between performances and CHD in the present study. First, heterogeneity of CHD and surgical procedures in the CHD subgroup may explain a lack of clear differences in cognitive performances in patients with and without CHD. Indeed, different CHD (Limperopoulos et al. 1999), different surgeries (Bellinger et al. 1995), or different ages at surgery (Newburger et al. 1984) have all been identified as important determinants of cognitive outcome. Second, it is possible that the effect of CHD on cognitive outcome, as we are able to measure it, may remain subtle in this genetic syndrome which is already associated with a consequent mental delay (i.e., without CHD). Even if we identified specific volumetric alterations related to CHD, these alterations may not be directly translated into isolated cognitive deficits. Similarly, Bellinger (2003) has discussed how pediatric cardiac surgery differs from adult surgery, pointing out that one occurs in a developmental and plastic setting, whereas the other is more likely to result in “static neuropsychological deficits”. Finally, the lack of clear relationship between CHD and intelligence in the entire sample of patient contrasts the difference in cognitive performances observed between adults with and without CHD. As Bellinger (2003) suggested, some deficits may remain silent until the child learns more complex tasks or, more optimistically, the difference between children and adults’ outcomes may rely on evolution in cardiac surgical procedures during the last decade.
In the present study, we propose that the distribution of reduced cortical volumes supports an impact of hemodynamic alterations both in 22q11DS and in CHD, opening avenues for exploring cerebrovascular malformations and their relationship with cognitive outcome in the syndrome. First, we evidenced a rostrocaudal and a lateromedial gradient of cortical reduction in 22q11DS, which may support a role for reduced blood supply in the territory supplied by the posterior cerebral artery. Second, this study is also the first to report changes to regional cortical volume related to CHD. Even if these changes occur within the wider context of the syndrome, we argue that they provide clues for furthering our understanding of the neurology of CHD and of the intellectual impairments often reported in children who underwent cardiac surgery.
This research was supported by Swiss National Research Funds (grant 323500-111165 to M.S. and grants 3200-063135, 3232-063134, and PP00B-102864 to S.E.), by NARSAD Institute, and by the Center for Biomedical Imaging of the Geneva, Lausanne Universities and the EPFL, as well as the foundations Leenaards and Louis-Jeantet (http://www.cibm.ch).
Open Access This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
Marie Schaer, Phone: +41-22-3886731, Fax: +41-22-3886769, Email: firstname.lastname@example.org.
Bronwyn Glaser, Email: email@example.com.
Marie-Christine Ottet, Email: firstname.lastname@example.org.
Maude Schneider, Email: email@example.com.
Meritxell Bach Cuadra, Email: firstname.lastname@example.org.
Martin Debbané, Email: email@example.com.
Jean-Philippe Thiran, Email: firstname.lastname@example.org.
Stephan Eliez, Email: email@example.com.