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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
Transplantation. Author manuscript; available in PMC 2010 November 5.
Published in final edited form as:
Transplantation. 1990 June; 49(6): 1170–1172.
PMCID: PMC2974268
NIHMSID: NIHMS246218

SPECIES DIFFERENCES IN SENSITIVITY OF T LYMPHOCYTES TO IMMUNOSUPPRESSIVE EFFECTS OF FK 5061

FK 506 is a novel immunosuppressive drug isolated from Streptomyces tsukubaensis in 1984 (1). Initial in vitro studies have indicated that FK 506 is a potent inhibitor of murine and human mixed lymphocyte reactions and can prevent the generation of cytolytic cells. FK 506 suppresses T cell–dependent responses and this effect appears to be mediated through the inhibition of interleukin-2 release and diminished expression of the IL-2 receptor on activated T cells (2, 3). The strong immunosuppressive effect of FK 506 has been observed during primary activation of lymphocytes, such as the mixed leukocyte reaction and the secondary proliferation of alloreactive T lymphocytes generated from MLR or propagated from organ transplant biopsies as measured by the primed lymphocyte test (4).

Although both FK 506 and cyclosporine exhibit similar mechanisms of action, the immunosuppressive effect of FK 506 is several-hundred-fold greater than that of CsA. Synergism between the two drugs has been observed in vivo and in vitro, as demonstrated by significant immunosuppressive effects of combinations of drugs at low doses that are individually ineffective (5, 6). Synergism has also been noted between FK 506 and azathioprine (7).

In vivo studies have also established the effectiveness of FK 506 in several allograft models. Todo et al. showed that FK 506 greatly prolongs allograft survival of heterotopic heart transplants in rats (810), and renal and liver transplants in dogs (11). In these experiments, FK 506 was found to be effective at dose levels of 1 – 1.5 mg/kg/day—however, preliminary studies with kidney transplants performed in baboons indicated that this dose of FK 506 was ineffective in prolonging graft survival (12). In vitro studies were conducted to quantitate the immunosuppressive effects of FK 506 on the MLR with lymphocytes from baboons, dogs, rats, and humans.

Heparinized blood was collected from normal dogs (n = 9), rats (n = 3), baboons (n = 10) and humans (n = 8). Mononuclear cells were isolated by Ficoll-Hypaque sedimentation and uni-directional MLR cultures were set up in triplicate with equal numbers (106/ml) of responders and irradiated stimulators in 10% autologous serum and incubated for 6 days at 37°C and 5% CO2. During the final 20 hr of incubation, each culture was labeled with 1 µCi of [3H]-thymidine (ICN Radiochemicals, 1 mCi/ml), harvested, and counted in a liquid scintillation counter. The dose effect of FK 506 and CsA on MLR activity was measured at different concentrations of the drug ranging from 0.03–10 ng/ml for FK 506 to 3.5–2500 ng/ml for CsA. For each culture the ID50 dose was calculated as the concentration of FK 506 or CsA that caused a 50% inhibition of the MLR.

Figure 1 illustrates, that the ID50 of FK 506 was about 10-fold higher for baboon lymphocytes (3.85±2 ng/ml) than for lymphocytes from the other three species (rats 0.33±0.2 ng/ml; humans 0.29±0.2 ng/ml; dogs 0.59±0.58 ng/ml). These differences in FK 506 sensitivity were statistically significant (P<0.001). Similar differences between ID50 values were observed for CsA, although their magnitudes were somewhat lower (Fig. 2). The ID50 of CsA was Significantly higher for baboons (212±161 ng/ml) than for rats (45±4 ng/ml); humans (50.5±28.5 ng/ml) and dogs (40.8±18.5 ng/ml). These data also extend previous observations that FK 506 is 100–300 times more potent in inhibiting MLR than CsA (4).

FIGURE 1
ID50 values for FK 506 in MLR cultures from rats, human, dogs, and baboons. Each point represents one experiment done in triplicate. Statistical analysis of the ID50 values was done using the unpaired Student's t test. The analysis was done for baboons ...
FIGURE 2
ID50 values for CsA in MLR cultures from rats, humans, dogs, and baboons. Each point represents one experiment done in triplicate.

Comparable differences in FK506 sensitivity were observed in vivo in different allograft models. Table 1 summarizes the survival rates of kidney transplants in baboons versus kidney transplants in dogs and cardiac transplants in rats. In the baboon and dog models FK 506 was administered orally, in the rat model the drug was administered intramuscularly. Successful prolongation of RT-I-incompatible cardiac allograft in rats was achieved at FK506 doses of 1.28 mg/kg/day with a mean survival of 99 days (10), whereas untreated allografts only survived 6 days. At daily doses of 1.5 mg/kg/day, FK 506 prolonged allogeneic renal transplants from mongrel to beagle dogs from 14 days to 61 days (11). However, a similar dose of FK 506 (2 mg/kg/day) induced a modest and insignificant prolongation of renal transplant survival in baboons (16 days versus 9 days for untreated controls). Considerably higher doses of FK 506 (12 and 18 mg/kg/day) were necessary for baboons to achieve allograft survivals comparable to those observed in rats and dogs at lower doses (Table 1).

TABLE 1
Effect of FK 506 on allograft survival in the three animal species

The results of these in vivo and in vitro studies demonstrate species differences in sensitivity in the immunosuppressive effects of FK 506. The baboon model requires almost tenfold higher doses of FK 506 than the rat and dog models to achieve optimal immunosuppression. The in vitro assays indicate that FK 506 sensitivity of human lymphocytes is more similar to that for dogs and rats rather than baboons. These findings suggest that FK 506 at doses of 1.5 mg/kg/day might provide effective immunosuppression of human allografts. However only clinical trials can establish the optimal administration of this drug.

In transplant patients the optimization of immunosuppressive treatment depends on the administration of appropriate doses that have minimal side effects and the monitoring of drug levels. Pharmacokinetic and pharmacodynamic evaluations have aided in developing individualized immunosuppressive therapy (13). Furthermore drug sensitivity of in vitro lymphocyte activation seems useful, and considerable variations have been seen between different individuals (14). In this study species differences in FK 506 sensitivity of MLR reflect the efficacy of FK 506 treatment in prolonging allograft survival. Thus in vitro studies of drug sensitivity of lymphocyte activation are useful in determining optimal drug doses to ensure successful transplantation.

Footnotes

1This work was supported by Grants AI-23467 and HL-36416 from the National Institutes of Health.

REFERENCES

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