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To determine whether there is a difference in outcome between different ovulation-induced cycles after frozen-thawed embryo transfer (FET).
We searched the Cochrane Menstrual Disorders and Subfertility Group’s trials register in May 2009, the Cochrane Central Register of Controlled Trials (Cochrane Library, Issue 1, 2008), ISI Web of Knowledge (1985 to August 2009), and reference lists of articles. Relevant conference proceedings were hand-searched and researchers in the field were contacted. Randomized controlled trials and retrospective studies were included, comparing the various cycle regimens and different methods during FET in assisted reproductive technology, ie, in vitro fertilization and intracytoplasmic sperm injection.
Using the agonist long protocol for downregulation, five trials provided extractable data for live-birth rates, ongoing pregnancy, and clinical pregnancy rates following FET. One trial provided extractable data for clinical pregnancy rate. There was no evidence of a significant difference in any outcome between the users of urinary gonadotropins versus recombinant follicle-stimulating hormone. Data on implantation and miscarriage rates following FET were not available for analysis.
It seems that clinical pregnancy rate after FET is not influenced by the type of gonadotropins used. Research should be directed towards improving freezing and thawing techniques.
Frozen-thawed embryo transfer (FET) has become a vital aspect of assisted reproduction.1 Pregnancy rates following FET treatment have always been lower than following fresh embryo transfer. Nevertheless, FET increases the cumulative pregnancy rate, reduces cost, is relatively simple to undertake, and can be accomplished in a shorter time period compared with repeated “fresh” cycles. FET is performed using different cycle regimens,2 ie, spontaneous ovulatory cycles, cycles in which ovulation is induced by drugs, and cycles in which the endometrium is artificially prepared using oestrogen and progesterone hormones, with or without a gonadotropin-releasing hormone (GnRH) agonist. Results of fresh and FET in women undergoing in vitro fertilization (IVF) with GnRH agonists and GnRH antagonists showed no significant difference between methods.3
Little is known about the impact of type of gonadotropins on FET. In the medical literature, recent meta-analyses have shown a significant advantage of using urinary gonadotropins over recombinant follicle-stimulating hormone (rec-FSH) during ovarian stimulation, with higher clinical pregnancy and live-birth rates in the former group.4,5 However, these reviews did not directly assess the results of using different FSH preparations in FET cycles, nor cumulative pregnancy and live-birth rates per stimulated cycle.
The beneficial effect of urinary gonadotropins has been investigated in the Menotropin versus Recombinant FSH in vitro Fertilisation Trial (MERiT), which showed differences in embryo quality and endometrial receptivity between rec-FSH and highly purified human chorionic gonadotropin (HP-hMG).6 Balasch et al7 compared follicular development and estradiol levels after ovarian stimulation in pituitary-suppressed, normally ovulating women undergoing IVF, using highly purified urinary FSH (u-FSH-HP) and rec-FSH. A secondary variable in their study was embryo implantation potential, which is closely related to appropriate follicular development and oocyte competence. They found that rec-FSH is more effective than u-FSH-HP when used in the same patient for inducing multiple follicular development in downregulated cycles, as indicated by ovarian performance and oocyte maturity. In addition, they concluded that rec-FSH yields significantly higher implantation rates than u-FSH-HP when used in patients undergoing their second IVF attempt.
Clinical efficiency in IVF procedures have been debated for years. Definition of a goal, or endpoint, for IVF treatments has varied between clinicians, regulatory bodies, and organizations, with some regarding a clinical pregnancy, an ongoing pregnancy, a live birth, or even a “take-home” baby as the primary outcome of IVF treatments.8–21 This controversy has recently been complicated by the use of single embryo transfer to reduce the incidence of multiple births. This has added another variable to the equation, ie, whether a multiple gestation pregnancy should be considered a success or a failure. Regardless of the exact definition of success, the use of FET has undoubtedly led to more success in assisted reproduction. The objective of this systematic review and meta-analysis was to determine the effectiveness of the use of urinary gonadotropins versus rec-FSH in FET.
In order to determine the effectiveness of cryo-embryo transfers following ovarian stimulation with urinary gonadotropins compared with rec-FSH, we included comparative trials (ie, randomized controlled trials, nonrandomized controlled trials, cohort studies [prospective or retrospective], and case-control studies). Study designs were classified according to their methodological rigor, with lower levels of evidence included only if there was a lack of adequate higher quality evidence. Adequacy of evidence was determined after taking into consideration the number and sample sizes of the included studies at each level of evidence.
Participants in the included studies were women who were part of a couple, underwent assisted reproduction, ie, IVF or intracytoplasmic sperm injection (ICSI) for any cause of infertility, and underwent cryo-embryo transfer. Women undergoing transfer from donor oocytes and/or embryos were excluded. Duration, type (ie, primary or secondary), and cause of infertility were not considered as separate confounders, although they are presented descriptively in the overview of studies, along with all other possible confounding factors.
Trials comparing women undergoing cryo-embryo transfer following ovarian stimulation with urinary gonadotropins versus rec-FSH were included. All dosages and durations of stimulation were included, as were different freezing and thawing techniques. Details of ovarian stimulation protocols, and freezing/thawing cycles are presented in the overview of studies.
The primary outcome measures for this review were clinical pregnancy and miscarriage rates following cryo-embryonic transfer per woman. Secondary outcomes were implantation, ongoing pregnancy, and live-birth rates following cryo-embryonic transfer. Additionally, the cumulative clinical pregnancy and live-birth rates per woman were evaluated. The cumulative rates were calculated by combining the pregnancy rates from the fresh and frozen-thawed cycles, when separately presented. In a subgroup analysis, the results for each group (eg, women with fresh cycles and women with frozen-thawed cycles) were also analysed separately from each other.
We electronically searched the Medline, Embase, and Central ISI Web of Knowledge databases from 1985 to August 2009, as well as the Cochrane Library, Issue 3, 2009, with no language barriers. We further performed hand searches of conference abstracts of major proceedings (eg, European Society of Human Reproduction and Embryology and American Society of Reproductive Medicine) for the past five years, and the reference lists of expert-identified major review articles relevant to the present review and, in turn, the reference lists of included trials. We also searched prospective trial registers of, eg, the World Health Organization (WHO), the ClinicalTrials.gov website, and the International Standard Randomized Controlled Trial Number (ISRCT) Registry, for citations of ongoing and unpublished trials.
Individual search strategies were developed for each database using the following MeSH terms, keywords, and a modified version of the Cochrane Highly Sensitive Search Strategy (HSSS): urinary, recombinant, follicle stimulating hormone, FSH, gonadotropin, gonadotropin, human menopausal gonadotropin, human menopausal gonadotropin, urinary FSH, u-FSH, hMG, recombinant FSH, rec-FSH, frozen embryo transfer, frozen embryo replacement, frozen embryo cycle, FET, clinical pregnancy rate, and brand names of drugs used in treatment.
In this meta-analysis, live birth rate after FET was the primary parameter. Several studies only present cumulative pregnancy rates (ie, their results also included pregnancy rates for fresh embryo transfers). For sound statistical comparison, fresh and FETs should be analysed separately. The effects of urinary gonadotropins and rec-FSH on the fresh embryo transfers have already been demonstrated in many studies, ie, that there is no significant difference in pregnancy rates. To isolate the pregnancy rate for FET from the cumulative pregnancy rate, the flow diagram shown in Figure 1 can be followed.
Of all the n patients (n1 in u-FSH and n2 in rec-FSH) who underwent controlled ovarian hyperstimulation in the study, m patients (m1 in u-FSH and m2 in rec-FSH) underwent oocyte retrieval, of whom p patients had oocytes retrieved (p1 in u-FSH and p2 in rec-FSH). From these patients, a total of q oocytes were obtained and inseminated via regular assisted reproductive technology (ART) procedures: (q1 in u-FSH and q2 in rec-FSH). The flow of oocyte/embryo assessments is illustrated in Figure 1. A total number of r embryos are transferred in the fresh cycles (r1 in u-FSH and r2 in rec-FSH), and s embryos are cryopreserved (s1 in u-FSH and s2 in rec-FSH). During a certain period, t embryos are thawed (t1 in u-FSH and t2 in rec-FSH), of which u thawed embryos are transferred (u1 in u-FSH and u2 in rec-FSH) in v patients (v1 in u-FSH and v2 in rec-FSH) and w cryocycles (w1 in u-FSH and w2 in rec-FSH). Finally, the clinical pregnancy rate per frozen-thawed embryo cycle can be derived. In several studies, other units were used for the pregnancy rate, or even omitted. Some authors present only the live birth (or baby take-home) rates. Other authors present the data over a one-year period, instead of a cycle.
Data collection and analysis was conducted in accordance with guidelines presented in the Cochrane Handbook for Systematic Reviews of Interventions.22 A data set (N1, N2, M1, M2) was extracted from each study, in which N1 and N2 subjects in the rec-FSH and urinary gonadotropins groups, respectively, underwent a cycle during which frozen-thawed embryos were replaced, resulting in M1 pregnancies in the rec-FSH group and M2 in the urinary gonadotropins group.
Dichotomous outcomes (eg, clinical pregnancy rates) were expressed as an odds ratio (OR) with 95% confidence intervals (CI) for each study. Results from each study were meta-analysed with the Comprehensive Meta-Analysis software, version 2.2046 (Biostat, Englewood, NJ) using the Mantel-Haenszel method with a fixed-effect model.
A standardized data extraction form was developed and piloted for consistency and completeness. Trials were considered for inclusion and trial data were extracted by one review author (PVG), and checked for accuracy and completeness by a second reviewer (HAI) according to the inclusion criteria, with conflicts being resolved by consensus. In the event of missing data, corresponding authors of included studies were contacted for additional information.
The analysis of dichotomous data and continuous data was performed using a random-effects model, and the OR and weighted mean difference, respectively, evaluated with their 95% CI. P values are presented for further confirmation of the results. A P value of <0.05 was considered to be statistically significant.
In the graphical display of the analyses, an increased probability of a favorable outcome (ie, clinical pregnancy) for u-FSH would be displayed graphically to the right of the center line and vice versa. On the contrary, an increased probability of not having a detrimental outcome (eg, miscarriage) from u-FSH would be displayed graphically to the left of the center line.
Given that clinical infertility trials should ideally deal with women and not cycles, a woman should only be included once (ie, one cycle per woman) in the trial, otherwise there would be a unit-of-analysis error. Common statistical errors in the design and analysis of subfertility trials have already been reported,23 and the typical unit-of-analysis errors in fertility trials are well described. The authors found that 82% of the 39 trials which they investigated contained at least one unit-of-analysis error, but still passed review. Other unit-of-analysis issues have also been addressed by Van Gelder.24 Each study in the underlying meta-analysis was critically reviewed, and errors and omissions identified by Vail and Gardener23 were checked.
The classical measure of heterogeneity is Cochran’s Q which is the weighted sum of squared differences between individual study effects and the pooled effect across studies. Also, the H and I2 statistics have been calculated and included in the assessment. A large value of Q indicates that there is significant heterogeneity between studies. The significance level for this statistic was set to 0.10 rather than the usual 0.05. The I2 test describes the percentage of the variability in effect estimates attributable to heterogeneity rather than sampling error (chance), with an I2 value >50% considered to represent substantial heterogeneity.25
Publication bias was assessed by visually noting the scatter of the point estimates and CIs for each study and by the Trim and Fill method when there were at least 10 studies in an analysis. Since the number of included studies in all of the analyses was below 10, the assessment was cancelled. No subgroup analysis was carried out in this meta-analysis.
A conventional approach was used to calculate the minimum required sample size with four parameters: Type I error, power, assumptions in the first group, and expected treatment effect. Type I error and power were fixed at the conventional levels (5% for Type I error, 80% for power). Assumptions related to the first group were prespecified on the basis of previously observed data (a clinical pregnancy rate of FET per randomized woman of 25%), and an expected treatment effect of 5% was expected to be clinically meaningful. With these assumptions, the sample size required was 1290 for group 1 and 1290 for group 2. None of the analyses reached this level of confidence because of the lack of available data in the literature.
Electronic and hand searches identified 265 potentially relevant citations based on title/abstract screening. Of these, five Cochrane reviews were identified,2,26–29 two descriptive studies of FET,30,31 and one meta-analysis.5 In total, 247 citations were excluded, and 18 citations to comparative studies were further evaluated using the full-text manuscripts.32–49 Finally, only five studies met the inclusion criterion.37,38,42,46,48
Before the five trials are discussed, a brief summary is presented of a number of papers which did not fulfill the inclusion criteria, but which provide interesting data on FET.
Oehninger et al41 presented a retrospective study with more than 9000 embryos which had been cryopreserved in gonadotropin-stimulated in vitro IVF cycles. Over 1500 thaw and transfer cycles yielded a pregnancy rate of approximately 25%. Different ovarian stimulation regimens (various preparations of FSH, alone or in combination with hMG, with or without concomitant use of a GnRH agonist) did not influence embryo survival or pregnancy rate. Likewise, the application of oocyte/embryo micromanipulation techniques for assisted fertilization (ICSI for male infertility) or assisted hatching (performed selectively) did not have an impact on pregnancy results. Pregnancy outcome following embryo cryopreservation/thawing in IVF cycles stimulated with a combination of a GnRH agonist (long protocol) and FSH (urinary, urinary highly purified, or recombinant) were reported.41
In a paper by Francsovits et al,34 a total of 389 oocytes were collected in a human follicle stimulating hormone group and 415 oocytes in an rec-FSH group. Part of the embryos were cryopreserved and clinical pregnancy rates were similar in both groups (13/35 versus 11/34, respectively).
Gerli et al,36 studied 60 IVF patients undergoing embryo transfer for the first time and randomly allocated them into two groups. Group A (n = 30) had subcutaneous administration of u-FSH (Fostimon 75, AMSA, Italy). Group B (n = 30) had subcutaneous administration of u-FSH (Metrodin 75 HP, Serono, Italy). This prospective randomized clinical study in an IVF embryo transfer program showed that both drugs were equally safe and effective. The two groups did not differ in clinical pregnancy rates per attempt and per transfer. When frozen embryo cycles were included, the difference in pregnancy rate became significant.
In a study by Salumets et al,45 FET was characterized by impaired pregnancy outcome and increased incidence of pregnancy loss compared with fresh IVF/ICSI embryo transfers. This study reviewed the outcome of 1242 FETs with respect to the age of the woman, the method of fertilization, embryo quality before and after freezing, and the number of embryos transferred. These data revealed that the delivery rate after FET depended on both the woman’s age and the quality of embryos transferred, at the same time being unaffected by IVF/ICSI treatment. Ovarian stimulation was performed using either human menopausal or rec-FSH after suppression was achieved.
Lieberman et al40 reported that live birth rates were similar for urinary and recombinant gonadotropin preparations. They also reported that the implantation rates for frozen embryos were not significantly lower than for fresh embryos. However, no details were given about whether ovarian stimulation was performed using human menopausal or rec-FSH.
Wang et al47 presented a retrospective study analysing 3570 FET cycles (involving 1438 couples) with a view to increasing our understanding of the clinical circumstances that influence the potential for embryo implantation. The overall implantation rate was 9.1%. The characteristics associated with a more favorable implantation rate were the success of the previous fresh embryo transfer cycle, age <40 years, and nontubal factor aetiology of infertility. However, no details were given about whether the ovarian stimulation was performed using human menopausal or rec-FSH.
Oktay et al50 carried out a meta-analysis of ICSI cycles with frozen-thawed oocytes in comparison with a matched-control group undergoing IVF-ICSI with fresh oocytes in a large ART program. They report that clinical and live-birth pregnancy rates per embryo transfer with oocyte cryopreservation are lower compared with success rates with fresh oocytes. However, no details were given about whether the ovarian stimulation was performed using human menopausal or rec-FSH.
Gelbaya et al35 assessed the implantation, pregnancy, and live-birth rates after FET in a natural or hormonal control cycle. Two comparable groups of women with regular menstrual cycles: group A (n = 212) had FET in a natural cycle after spontaneous ovulation; group B (n = 205) had FET in a pituitary-desensitized hormonally controlled cycle. In group B, a GnRH agonist was commenced in the mid-luteal phase of the previous cycle and discontinued three days before embryo transfer. Oral estradiol valerate and a vaginal progesterone pessary were used to prepare the endometrium. Embryo transfer was carried out three days after detection of the endogenous luteinising hormone surge in group A and on day three of progesterone administration in group B. In the 212 women who had natural-cycle FET, 172 embryo transfers were performed and 247 embryos replaced. The implantation rate was 14.1% (35/247). Twenty clinical pregnancies (20/172, 11.6%) were achieved. In the 205 women who had downregulated hormone replacement cycle FET, 173 embryo transfers were performed and 243 embryos replaced. The implantation rate was 13.5% (33/243). Eighteen clinical pregnancies (18/173, 10.2%) were achieved. There were no significant differences between the two groups with regard to the implantation, clinical pregnancy, or live-birth rates per cycle or per embryo transfer. The findings of Gelbaya et al35 suggest that both FET protocols are equally effective in terms of implantation rate and pregnancy outcome in women with regular menstrual cycles. However, no details were given about whether the ovarian stimulation was performed using human menopausal or rec-FSH.
Racowksy et al44 compared the cumulative pregnancy rates for fresh and frozen-thawed embryos and found no significant difference between rec-FSH and u-FSH in clinical pregnancy rates per started cycle [44.1 (367/832) versus 43.6% (248/569)], or delivery rates [37.9% (315/832) versus 35.5% (202/569)].
Using the agonist long protocol for downregulation, five trials provided extractable data for this review and meta-analysis. The characteristics of these trials are discussed below in detail.
Ziebe et al48 evaluated as part of a randomized, assessor-blind, multinational trial conducted in 731 women undergoing IVF after stimulation with highly purified human menopausal gonadotropin (HP-hMG; MENOPUR, n = 363) or rec-FSH (rec-FSH; GONAL-F, n = 368). Ongoing pregnancy was the primary endpoint [HP-hMG 27% and rec-FSH 22%; OR (95% CI) 1.25 (0.89–1.75)]. The live-birth, ongoing pregnancy, and ongoing implantation rates for top quality embryos were higher with HP-hMG than rec-FSH [48 versus 32% (P = 0.038), 48 versus 32% (P = 0.038), 41 versus 27% (P = 0.032), respectively]. Both the proportion of embryos with at least 50% surviving blastomeres after cryopreservation and of embryos resuming mitosis were more frequent with HP-hMG than with rec-FSH. A one-year followup of the cryopreserved embryos included data for 178 patients (HP-hMG 89, rec-FSH 89) who had embryos thawed in the specified time period, of whom 142 patients (HP-hMG 69, rec-FSH 73) underwent 206 embryo transfer cycles (HP-hMG 93, rec-FSH 113) using 331 embryos (HP-hMG 145, rec-FSH 186). In the first cryocycle, the live-birth rate was 9% in both the HP-hMG and rec-FSH groups. The live birth rate in the cryocycles with embryo transfer was 13% for HP-hMG and 10% for rec-FSH.
Seelig et al46 carried out a retrospective study with hMG (n = 194) and recFSH (n = 92) in a long-protocol or hMG (n = 16) or recFSH (n = 40) stimulation under pituitary suppression with the GnRH antagonist cetrotide. The two-pronuclear oocytes were transferred after endometrial preparation. Implantation rates in the freeze-thaw cycles were 5.6% (hMG) and 3.8% (rec-FSH) with two-pronuclear oocytes from the long protocol, and 7% from the antagonist cycles, irrespective of whether hMG or recFSH was used. Pregnancy rates were similar, independent of whether they resulted from the long protocol cycles with hMG (15.4%) or recFSH (13.1%), or from the antagonist protocol cycles with hMG (25.0%) or recFSH (17.5%).
Out et al42 conducted a prospective, randomized, assessor-blind, multicenter (n = 18) study with infertile women undergoing IVF comparing rec-FSH (Org 32489, Puregon®) and u-FSH (Metrodin®). In total, 585 subjects received rec-FSH and 396 u-FSH. Ongoing pregnancy rates per attempt and transfer in the rec-FSH group were 22.17% and 25.97%, respectively, and in the u-FSH group, 18.22% and 22.02%, respectively. These differences were not statistically significant. Ongoing pregnancy rates including pregnancies resulting from frozen-thawed embryo cycles were 25.7% for rec-FSH and 20.4% for u-FSH (P > 0.05). Subsequently, 117 and 73 subjects in the rec-FSH and u-FSH groups, respectively, underwent a natural cycle during which frozen-thawed embryos were replaced, resulting in 17 ongoing pregnancies in the rec-FSH group and five in the u-FSH group. A second “frozen embryo” cycle was done in 26 and 15 women (“new women”, not coming from the previous group of 117 and 73 women, respectively, who did not become pregnant), which resulted in seven additional pregnancies (five in the rec-USH and two in the u-FSH group). Eight women had a third frozen embryo cycle and two subjects a fourth, which did not result in ongoing pregnancies. The mean number of embryos transferred in the frozen embryo cycles was 2.1 for both groups. In total, 22 additional pregnancies were obtained in the rec-FSH group, and seven in the u-FSH group, resulting in cumulative ongoing pregnancy rates (adjusted for center) of 25.7% and 20.4% in favor of rec-FSH.
Kahn et al38 carried out a prospective, randomized, comparative, cohort study which addressed issues of pregnancy rate per couple over several cycles of treatment and the inclusion of pregnancies from frozen embryos resulting from the study cycles. In this trial, the take-home baby rate over three cycles of IVF treatment, including those babies conceived from frozen embryos, was 54/86 (62.8%) for women using rec-FSH (follitropin-beta) and 40/60 (66.7%) for those using u-FSH (P = 0.63).
Hompes et al37 carried out an open-label, prospective, randomized study in which they studied the ongoing pregnancy rates per started cycle of 629 patients, and reported rates of 26.3% and 25.2% for HP-hMG and rec-FSH, respectively (no statistically significant difference). Within the groups, the numbers of subjects with embryo transfer in cryo-cycles 1 and 2 were 19 and 1, respectively, in the HP-hMG group and were 31 and 4, respectively, in the rec-FSH group. The numbers of ongoing pregnancies in these cryocycles were 5/20 (25%) in the HP-hMG group and 4/35 (11.4%) in the rec-FSH. No statistically significant differences were observed for the ongoing pregnancy rates in the cryocycles. The same applies to the cumulative ongoing pregnancy rates, cumulative delivery rate, and cumulative live-birth rate (combining results of collecting cycle and one-year outcome of cryocycles).
Figure 2 shows the results from the meta-analysis of the above five studies, which show no evidence of a significant difference between the use of u-FSH versus rec-FSH regarding the live-birth rate (u-FSH 6/69 versus rec-FSH 7/73, OR = 0.90; 95% CI = 0.29–2.82, P = 0.85) and the ongoing pregnancy rates (u-FSH 5/73 versus rec-FSH 17/117, OR = 0.43; 95% CI = 0.15–1.23, P = 0.12). Data on the clinical pregnancy, implantation, and miscarriage rates following FET were not available from the randomized trials. The cumulative live-birth rate did not demonstrate any significant differences between the use of u-FSH versus rec-FSH for ovarian stimulation, according to Ziebe et al48 and Hompes et al37 [(u-FSH 105/363 versus rec-FSH 91/368, OR = 1.24; 95% CI = 0.89–1.72, P = 0.20), (u-FSH 82/312 versus rec-FSH 75/317, OR = 1.15; 95% CI = 0.80–1.65, P = 0.45), respectively].
The cumulative ongoing pregnancy rate also did not demonstrate any significant differences between the use of u-FSH versus rec-FSH for ovarian stimulation, according to Hompes et al37 and Out et al42 [(u-FSH 82/312 versus rec-FSH 80/317, OR = 1.06; 95% CI = 0.74–1.51, P = 0.76), (u-FSH 150/585 versus rec-FSH 87/396, OR = 1.22; 95% CI = 0.91–1.66, P = 0.19), respectively]. Also, the cumulative clinical pregnancy rates per woman did not demonstrate any significant differences between the use of u-FSH versus rec-FSH for ovarian stimulation, (u-FSH 20/60 versus rec-FSH 37/86, OR = 0.66; 95% CI = 0.33–1.31, P = 0.24).
The retrospective study by Seelig et al46 provided data on the clinical pregnancy rate following the use of u-FSH versus rec-FSH in women undergoing ovarian stimulation in agonist and antagonist cycles. For women undergoing a long agonist protocol, the clinical pregnancy rate showed no evidence of a significant difference in effect between the use of u-FSH versus rec-FSH (u-FSH 30/195 versus rec-FSH 12/92, OR = 1.21; 95% CI = 0.59–2.49, P = 0.60), respectively. Using the antagonist protocol, the clinical pregnancy rates were also similar between the two groups (u-FSH 4/16 versus rec-FSH 7/40, OR = 1.57; 95% CI = 0.39–6.34, P = 0.53, Figure 2).
The results of this systematic review of the literature reveal no evidence of a significant difference between the clinical results following FET produced from stimulation cycles using u-FSH versus rec-FSH. It is also of importance that this review has demonstrated the dearth of information available in the literature on this subject, with most of the identified studies comparing only the transfer of embryos from the initial cycle, with minimal information regarding clinical outcomes following the transfer of frozen-thawed embryos.
Comparisons of different gonadotropin preparations regarding efficacy and safety as well as cost effectiveness are important issues in assisted reproduction. The cumulative pregnancy rate per cycle is strongly related to the number of oocytes retrieved, and therefore number of embryos available for transfer.51 Traditionally, ovarian stimulation protocols relied heavily on high doses of FSH to stimulate the largest cohort of follicles possible. This created two negative aspects of stimulation, a higher risk for the development of ovarian hyperstimulation syndrome, and an increased number of embryos that must either be transferred in the immediate cycle or discarded.52 Incorporation of cryopreservation of embryos has negated the latter because surplus embryos can be safely stored for future use. In centers where embryo cryopreservation is routinely used, almost 50% of all implantations are done by FET.53 Therefore, trials of FSH efficacy should include the cumulative success rates following FET per cycle started instead of only embryos transferred in the first cycle.20,54
Over the past few years, the efficacy of different FSH preparations have been tested to determine the most effective and safe product for use in human IVF. Today, the commercially available choices can be classified into highly-purified gonadotropins, which contain both FSH and luteinizing hormone, and HP-FSH and rec-FSH preparations. Due to the advanced purification and manufacturing processes, the subcutaneous route of administration is available for all these products, which makes them more convenient and patient friendly.
In clinical trials, rec-FSH had been proposed to be clinically more efficient than u-FSH (eg, Metrodin-HP, Menogon).55 However, these products have been replaced with newer compounds with a higher concentration of glycosylated FSH (Fostimon®) and in the case of gonadotropins, the addition of hCG. These modifications have shown that no single product has demonstrated clinical superiority over another product.4,5,56
Different aspects that may affect the implantation rate of frozen-thawed embryos have been tested over the years, including the use of GnRH agonist versus antagonist during ovarian stimulation, the type and components used during cryopreservation, and thawing techniques.57 Even so, to the best of our knowledge, and as demonstrated by the meticulous search of the literature, there are only a handful of studies that have investigated the effect of the type of FSH used during ovarian stimulation.
It seems that clinical pregnancy rate after FET is not influenced by the type of gonadotropins used. With respect to cumulative rates, it is noted that no significant differences in live-birth rate, ongoing pregnancy rate, and clinical pregnancy rate for fresh cycles and no significant differences in these rates for cryocycles implies no significant differences in the cumulative rates. Research should be directed to improving freezing and thawing techniques. At the present time there is insufficient evidence to support the use of one gonadotropin in preference to another. This review concludes that there is insufficient evidence to support the use of rec-FSH over urinary gonadotropins in ovarian stimulation prior to FET for women undergoing assisted reproductive technology.
The authors kindly acknowledge Goodlife Healthcare BV in The Netherlands for the financial support that led to this publication. The research also resulted in a systematic review, which was accepted for publication.58 There was no influence from the sponsors on the outcomes of these studies.