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Assay of the aTRE reporter in the developing chick retina.
A–D. Embryonic day 5 retinae were electroporated with aTRE + pβactin-RFP/GAPDH-shRNA in varying T3 concentrations. aTRE + GAPDH-shRNA -T3 (A, A'), 100 pM T3 (B, B'), 1 nM T3 (C, C'), and 100 nM T3 (D, D'). E–F. Cryosectioning of retina. 20 um cryosection of aTRE + GAPDH-shRNA -T3 (E, E') and 100 nM T3 (F, F'). G–H. TRα and TRβ shRNA in 100 nM T3. aTRE in 100 nM T3 + TRα-shRNA (G, G') or TRβ-shRNA (H, H').