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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
Gastroenterology. Author manuscript; available in PMC 2010 October 26.
Published in final edited form as:
Gastroenterology. 1964 July; 47: 61–64.
PMCID: PMC2964278


Macrocytic anemia due to stasis of the contents of the small intestine is a well known clinical entity. It has been reported in association with improperly drained intestinal segments at all levels of the small bowel, including partially obstructed afferent loops after gastrectomy,1 multiple jejunal diverticula,13 and with poorly draining ileal segments.4, 5

There is now strong evidence that bacterial overgrowth in such intestinal segments plays a major role in the etiology of the hematologic syndrome.2, 47 At present, however, the exact mechanism by which this bacterial proliferation leads to a malabsorption or malutilization of vitamin B12 is still not clear. In an effort to throw further light on this matter, segments of jejunum were interposed into the colon. We hoped by this technique to provide some information on two separate but related aspects the relationship of bacterial proliferation to malabsorption. First, because the segment of small intestine subjected to continuous fecal contamination would be out of the upper alimentary stream, any defect in absorption of vitamin B12 could then be considered to occur as a result of a circulating substance such as a toxin. Second, it could be observed whether prolonged bacterial contamination of the mucosa of the upper intestinal tract could cause recognizable histologic changes suggestive of a malabsorptive defect.

Materials and Method

Healthy, dewormed mongrel dogs were used. During the study, the animals were fed 1 can of Pard daily to which 100 gm. of lard was added. This high fat diet was given as part of a study which will be reported separately. All animals handled the diet well and none developed steatorrhea by actual stool f a t measurement. On the eighth day of this diet, each animal was fed a capsule containing 0.67 μg. of vitamin B12 tagged with radioactive cobalt 57. The radioactivity of these capsules had been previously measured in a large crystal scintillation counter. After ingestion of the capsule, a total stool collection was carried out over the next 72 hours while the animal was maintained on the same diet. The radioactivity of the total stool sample was then measured. Collections over a longer period of time failed to yield a significantly increased amount of tracer material. The amount of vitamin B12 excreted was then calculated as a percentage of that given.

Ten unoperated animals were used as controls. Three animals had a 30 cm. segment of upper jejunum interposed into the colon, and 3 animals had a 60 cm. segment interposed. The isolated segment on its mesentery was interposed isoperistaltically in an end-to-end fashion with a standard 2-layer technique (fig. 1). After recovering from surgery, the animals were sent to the animal farm for 3 to 4 months. Upon return to the laboratory, similar metabolic studies were again carried out. The animals were reexplored and biopsy specimens were taken from the transplanted segment.

Fig. 1
Segment of jejunum on its mesentery interposed into colon.


In the 10 control animals, average B12 excretion in the stool collection was 28 per cent (S.D., 7.6) of the ingested dose. This is similar to the figures of 33 per cent8 and 10 to 47 cent9 excretion reported in normal humans.

In group 1, those animals with 30 cm. interposition, both pre- and postoperative B12 excretions were all within the normal range. The animals with 60 cm. interpositions had similar results (table 1).

Fecal excretion of a test dose of cobalt labeled vitamin B12 in intact dogs and in dogs with ileal segments interposed in the colon

At reexploration, the interposed loops were filled with fecal contents as expected. Histologic study of the contaminated jejunum failed to reveal any consistent or marked change in the histologic pattern of the jejunal mucosa.


The anemia associated with stasis in the upper intestinal tract was investigated by Seyderhelm10 in 1924. He produced partial obstruction by suturing a band of the abdominal wall fascia around the terminal ileum, and postulated that the anemia thus produced was due to autointoxication from the chronically distended bowel. Several years later, Tonnis4 suggested that overgrowth of bacteria in the chronically obstructed loop might be important as a cause of the anemia.

More recently, Toon and Wangensteen7 and Watson and Witts,11 by using rats with surgically created blind loops as the experimental animal, were able to offer strong evidence that bacterial overgrowth in the loops was intimately associated with the ensuing macrocytic anemia. There has been no lack of clinical support for this concept. The reports of Goldstein,2 Scudamore,3 and, Adams1 are particularly important, because, in their cases, there is no possibility of the alimentary stream bypassing a significant portion of the terminal ileum which is now known to he the primary site of vitamin B12 absorption.12

The exact mechanism by which bacterial overgrowth may interfere with the absorption or utilization of vitamin B12 is not clear. One theory holds that there is bacterial competition for the available vitamin B12.

A second popular theory and one that is under investigation here is that a bacterial toxin is elaborated in and absorbed from the blind intestinal loop.13 Presumably, such a toxin might act to block B12 metabolism within the hematopoetic tissues.14, 15% Clinical data, however, support the concept that the block is at the absorptive rather than the utilization level. Because of this, it has been suggested 16 that a circulating toxin may somehow interfere with the ability of the mucosal surface of the ileum to properly absorb vitamin B12. Because it is known that the bacterial flora of the stagnant loop is similar to that of the colon,2 , 11, 17 it was hoped that interposing a segment of jejunum into the colon would demonstrate whether bacterial overgrowth in such a length of small intestine alone could interfere with the absorption of vitamin B12. If this occurred, it would have to be by means of a circulating toxin because the remainder of the small intestine was not exposed to any spillover of bacteria from the contaminated loop.

It is evident from the results of this study that if a toxin is elaborated, it is not produced by organisms normally present in the colon under the conditions of this experiment.

Although there are recognizable histologic changes present in many of the malabsorption states,18, 19 the role that bacteria play, if any, in these morphologic changes is still not certain. Some authors have felt that antibiotics, by virtue of altering the intestinal flora, were helpful in tropical spruce.20 Others have not been able to reproduce the same results in such a striking fashion.21, 22 With the administration of neomycin, Jacobson et al.23 produced a malabsorptive syndrome associated with changes in jejunal morphology suggestive of early spruce. The relationship of their findings to changes in intestinal flora is uncertain, however. More recently, Schiffer24 reported a case of “blind loop syndrome” associated with multiple diverticulitis of the jejunum in which peroral biopsy showed findings resembling those of early tropical spruce and celiac disease.

Thus, there is apparently at this time no clear cut agreement on whether changes in bacterial flora alone can lead to abnormalities in mucosal morphology. Multiple sections of jejunum from each animal in this study failed to reveal any abnormality even though they had been under continuous fecal contamination for upwards of 4 months.


An attempt was made to see if continuous massive bacterial contamination of the upper jejunum would adversely affect the absorption of vitamin B12 or cause recognizable mucosal histologic changes, or both. This was done by interposing jejunal segments into the colon for periods of up to 4 months. Vitamin B12 uptake by the fecal excretion method before and after this operation was measured. No effect on vitamin B12 absorption was evident. There was, in addition, no apparent effect on jejunal morphology of the interposed segment grossly or microscopically.


The work reported upon in this paper was supported in part by United States Public Health Service, Grant A-6283, United States Public Health Service, Washington, D. C.


1. Adams JF. Postgastrectomy megaloblastic anemia and the loop syndrome. Gastroenterologia. 1958;89:326. [PubMed]
2. Goldstein F, Wirts CW, Kramer S. The relationship of afferent limb stasis and bacterial flora to the production of postgastrectomy steatorrhea. Gastroenterology. 1961;40:47. [PubMed]
3. Scudamore HH, Hagendorn AB, Wollaegor EE, Owen CA. Diverticulosis of the small intestine and macrocytic anemia with report of two cases and studies on absorption of radioactive vitamin B12. Gastroenterology. 1958;34:66. [PubMed]
4. Siurala M, Kaipainen WJ. Intestinal megaloblastic anemia, treated with aureomycin and terramycin. Acta Med Scand. 1953;147:197. [PubMed]
5. Halsted JA, Lewis PM, Gasster MA. Absorption of radioactive Vitamin B12 in the syndrome of megaloblastic anemia associated with intestinal stricture or anastomosis. Amer J Med. 1956;20:42. [PubMed]
6. Donaldson RM., Jr Malabsorption of Co60-labeled cyanocobalamin in rats with intestinal diverticula. I. Evaluation of possible mechanisms. Gastroenterology. 1962;43:271. [PubMed]
7. Toon RW, Wangensteen OH. Anemia associated with blind intestinal segments and its prevention with aureomycin. Proc Soc Exp Biol Med. 1950;75:762. [PubMed]
8. Halsted JA, Lewis PM, Hvolboll EE, Gasster MA, Swendseid ME. An evaluation of the fecal recovery method for determining intestinal absorption of cobalt60-labeled vitamin B12. J Lab Clin Med. 1956;48:92. [PubMed]
9. Krevans JR, Conley CL, Sachs M. Influence of certain diseases on the absorption of vitamin B12 from the gastrointestinal tract. J Clin Invest. 1954;33:949.
10. Seyderhelm R, Lehman W, Wichels P. Experimental intestinal pernicious anemia of dogs. Klin Wschr. 1924;3:1439.
11. Watson GM, Witts LJ. Intestinal macrocytic anemia. Brit Med J. 1952;1:13. [PMC free article] [PubMed]
12. Booth CC, Mollin DL. The site of absorption of vitamin B12 in man. Lancet. 1959;1:18. [PubMed]
13. Reilly RW, Kirsner JB. The blind loop syndrome. Gastroenterology. 1959;37:421. [PubMed]
14. Drexler J. Effect of indole compounds on vitamin B12 utilization. Blood. 1958;13:239. [PubMed]
15. Ungley CC. Megaloblastic anemia following operations on intestine. Gastroenterologia. 1953;79:338. [PubMed]
16. Tonnis W, Brussis A. Changes of morphological blood picture in acute and chronic intestinal obstruction. Deutsch Z Chir. 1931;233:133.
17. Donaldson RM, Jr, Corrigan H, Natsios G. Malabsorption of Co60-labeled cyanocobalamin in rats with intestinal diverticula. II. Studies on contents of the diverticula. Gastroenterology. 1962;43:282. [PubMed]
18. Baker SJ, Ignatius M, Mathan VI, Vaish SK, Chacko CC. Ciba Foundation Study Group No. 14. Vol. 84. Little, Brown & Company; Boston: 1962. Intestinal Biopsy.
19. Rubin CE, Brandborg LL, Phelps PC, Taylor HC. Studies of celiac disease. I. The apparent identical and specific nature of the duodenal and proximal jejunal lesion in celiac disease and idiopathic sprue. Gastroenterology. 1960;38:28. [PubMed]
20. French JM, Gaddie R, Smith NM. Tropical sprue; a study of 7 cases and their response to combined chemotherapy. Quart J Med. 1956;25:333. [PubMed]
21. Sheehy TW, Perez-Santiago E. Antibiotic therapy in tropical sprue. Gastroenterology. 1961;41:208.
22. Reisher EH, Rosenblum C, Morgan MC. Urinary excretion of orally administered Co60 labeled vitamin B12 in normal subjects and patients with pernicious anemia and sprue. Clin Res Proc. 1954;2:56.
23. Jacobson ED, Prior JT, Faloon WW. Malabsorptive syndrome induced by neomycin:morphologic alterations in the jejunal mucosa. J Lab Clin Med. 1960;56:245. [PubMed]
24. Schiffer LM, Faloon WW, Chodos RB, Lozner EL. Malabsorptive syndrome associated with intestinal diverticulosis. Gastroenterology. 1962;42:63. [PubMed]