PMCCPMCCPMCC

Search tips
Search criteria 

Advanced

 
Logo of jcinvestThe Journal of Clinical InvestigationCurrent IssueArchiveSubscriptionAbout the Journal
 
J Clin Invest. 1991 July; 88(1): 315–322.
PMCID: PMC296035

Characterization of keratocalmin, a calmodulin-binding protein from human epidermis.

Abstract

Using affinity-purified calmodulin-binding proteins from human epidermis we have developed a monoclonal IgM antibody, ROC 129.1, to a human desmosomal calcmodulin-binding protein. This antibody reacts with a submembranous 250-kD protein from human keratinocytes and stains human epidermis in a "cell-surface pattern". Permeability studies indicated that the epitope with which this monoclonal reacts is on the inner surface of the cell membrane. Immunoelectronmicroscopy localized the antigen to the desmosome. The epitope is restricted to stratified squamous epithelia and arises between 8-12 wk of fetal development. This desmosomal calmodulin-binding protein, which we have termed keratocalmin, may be involved in the calcium-regulated assembly of desmosomes.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (3.0M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Hennings H, Michael D, Cheng C, Steinert P, Holbrook K, Yuspa SH. Calcium regulation of growth and differentiation of mouse epidermal cells in culture. Cell. 1980 Jan;19(1):245–254. [PubMed]
  • Hennings H, Holbrook KA. Calcium regulation of cell-cell contact and differentiation of epidermal cells in culture. An ultrastructural study. Exp Cell Res. 1983 Jan;143(1):127–142. [PubMed]
  • Boyce ST, Ham RG. Calcium-regulated differentiation of normal human epidermal keratinocytes in chemically defined clonal culture and serum-free serial culture. J Invest Dermatol. 1983 Jul;81(1 Suppl):33s–40s. [PubMed]
  • Ziboh VA, Isseroff RR, Pandey R. Phospholipid metabolism in calcium-regulated differentiation in cultured murine keratinocytes. Biochem Biophys Res Commun. 1984 Aug 16;122(3):1234–1240. [PubMed]
  • Jaken S, Yuspa SH. Early signals for keratinocyte differentiation: role of Ca2+-mediated inositol lipid metabolism in normal and neoplastic epidermal cells. Carcinogenesis. 1988 Jun;9(6):1033–1038. [PubMed]
  • Tang W, Ziboh VA, Isseroff R, Martinez D. Turnover of inositol phospholipids in cultured murine keratinocytes: possible involvement of inositol triphosphate in cellular differentiation. J Invest Dermatol. 1988 Jan;90(1):37–43. [PubMed]
  • Hennings H, Steinert P, Buxman MM. Calcium induction of transglutaminase and the formation of epsilon(gamma-glutamyl) lysine cross-links in cultured mouse epidermal cells. Biochem Biophys Res Commun. 1981 Sep 30;102(2):739–745. [PubMed]
  • Cheung WY. Calmodulin: an overview. Fed Proc. 1982 May;41(7):2253–2257. [PubMed]
  • Putkey JA, Ono T, VanBerkum MF, Means AR. Functional significance of the central helix in calmodulin. J Biol Chem. 1988 Aug 15;263(23):11242–11249. [PubMed]
  • Trinkaus-Randall V, Gipson IK. Role of calcium and calmodulin in hemidesmosome formation in vitro. J Cell Biol. 1984 Apr;98(4):1565–1571. [PMC free article] [PubMed]
  • Wallace RW, Lynch TJ, Tallant EA, Cheung WY. Purification and characterization of an inhibitor protein of brain adenylate cyclase and cyclic nucleotide phosphodiesterase. J Biol Chem. 1979 Jan 25;254(2):377–382. [PubMed]
  • Billingsley ML, Pennypacker KR, Hoover CG, Brigati DJ, Kincaid RL. A rapid and sensitive method for detection and quantification of calcineurin and calmodulin-binding proteins using biotinylated calmodulin. Proc Natl Acad Sci U S A. 1985 Nov;82(22):7585–7589. [PubMed]
  • Köhler G, Milstein C. Continuous cultures of fused cells secreting antibody of predefined specificity. Nature. 1975 Aug 7;256(5517):495–497. [PubMed]
  • Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. [PubMed]
  • Towbin H, Staehelin T, Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. [PubMed]
  • O'Farrell PH. High resolution two-dimensional electrophoresis of proteins. J Biol Chem. 1975 May 25;250(10):4007–4021. [PubMed]
  • Labib RS, Camargo S, Futamura S, Martins CR, Rock B, Anhalt GJ, Diaz LA. Pemphigus foliaceus antigen: characterization of a keratinocyte envelope associated pool and preparation of a soluble immunoreactive fragment. J Invest Dermatol. 1989 Aug;93(2):272–279. [PubMed]
  • Jones JC, Goldman AE, Steinert PM, Yuspa S, Goldman RD. Dynamic aspects of the supramolecular organization of intermediate filament networks in cultured epidermal cells. Cell Motil. 1982;2(3):197–213. [PubMed]
  • Watt FM, Mattey DL, Garrod DR. Calcium-induced reorganization of desmosomal components in cultured human keratinocytes. J Cell Biol. 1984 Dec;99(6):2211–2215. [PMC free article] [PubMed]
  • Jones JC, Goldman RD. Intermediate filaments and the initiation of desmosome assembly. J Cell Biol. 1985 Aug;101(2):506–517. [PMC free article] [PubMed]
  • Steinberg MS, Shida H, Giudice GJ, Shida M, Patel NH, Blaschuk OW. On the molecular organization, diversity and functions of desmosomal proteins. Ciba Found Symp. 1987;125:3–25. [PubMed]
  • Mattey DL, Suhrbier A, Parrish E, Garrod DR. Recognition, calcium and the control of desmosome formation. Ciba Found Symp. 1987;125:49–65. [PubMed]
  • Sheu HM, Kitajima Y, Yaoita H. Involvement of protein kinase C in translocation of desmoplakins from cytosol to plasma membrane during desmosome formation in human squamous cell carcinoma cells grown in low to normal calcium concentration. Exp Cell Res. 1989 Nov;185(1):176–190. [PubMed]
  • Tsukita S, Tsukita S. Desmocalmin: a calmodulin-binding high molecular weight protein isolated from desmosomes. J Cell Biol. 1985 Dec;101(6):2070–2080. [PMC free article] [PubMed]
  • Mueller H, Franke WW. Biochemical and immunological characterization of desmoplakins I and II, the major polypeptides of the desmosomal plaque. J Mol Biol. 1983 Feb 5;163(4):647–671. [PubMed]
  • Cowin P, Garrod DR. Antibodies to epithelial desmosomes show wide tissue and species cross-reactivity. Nature. 1983 Mar 10;302(5904):148–150. [PubMed]
  • Green KJ, Parry DA, Steinert PM, Virata ML, Wagner RM, Angst BD, Nilles LA. Structure of the human desmoplakins. Implications for function in the desmosomal plaque. J Biol Chem. 1990 Feb 15;265(5):2603–2612. [PubMed]
  • Koulu L, Kusumi A, Steinberg MS, Klaus-Kovtun V, Stanley JR. Human autoantibodies against a desmosomal core protein in pemphigus foliaceus. J Exp Med. 1984 Nov 1;160(5):1509–1518. [PMC free article] [PubMed]
  • Stanley JR, Koulu L, Klaus-Kovtun V, Steinberg MS. A monoclonal antibody to the desmosomal glycoprotein desmoglein I binds the same polypeptide as human autoantibodies in pemphigus foliaceus. J Immunol. 1986 Feb 15;136(4):1227–1230. [PubMed]
  • Eyre RW, Stanley JR. Human autoantibodies against a desmosomal protein complex with a calcium-sensitive epitope are characteristic of pemphigus foliaceus patients. J Exp Med. 1987 Jun 1;165(6):1719–1724. [PMC free article] [PubMed]
  • Roscoe JT, Diaz L, Sampaio SA, Castro RM, Labib RS, Takahashi Y, Patel H, Anhalt GJ. Brazilian pemphigus foliaceus autoantibodies are pathogenic to BALB/c mice by passive transfer. J Invest Dermatol. 1985 Dec;85(6):538–541. [PubMed]
  • Beutner EH, Prigenzi LS, Hale W, Leme C de A, Bier OG. Immunofluorescent studies of autoantibodies to intercellular areas of epithelia in Brazilian pemphigus foliaceus. Proc Soc Exp Biol Med. 1968 Jan;127(1):81–86. [PubMed]
  • Farb RM, Dykes R, Lazarus GS. Anti-epidermal-cell-surface pemphigus antibody detaches viable epidermal cells from culture plates by activation of proteinase. Proc Natl Acad Sci U S A. 1978 Jan;75(1):459–463. [PubMed]
  • Schiltz JR, Michel B, Papay R. Pemphigus antibody interaction with human epidermal cells in culture. J Clin Invest. 1978 Oct;62(4):778–788. [PMC free article] [PubMed]
  • Schiltz JR, Michel B, Papay R. Appearance of "pemphigus acantholysis factor" in human skin cultured with pemphigus antibody. J Invest Dermatol. 1979 Dec;73(6):575–581. [PubMed]
  • Kawana S, Geoghegan WD, Jordon RE. Complement fixation by pemphigus antibody. II. Complement enhanced detachment of epidermal cells. Clin Exp Immunol. 1985 Sep;61(3):517–525. [PubMed]
  • Kawana S, Geoghegan WD, Jordon RE. Complement fixation by pemphigus antibody. III. Altered epidermal cell membrane integrity mediated by pemphigus antibody and complement. J Invest Dermatol. 1986 Jan;86(1):29–33. [PubMed]
  • Lane AT, Helm KF, Goldsmith LA. Identification of bullous pemphigoid, pemphigus, laminin, and anchoring fibril antigens in human fetal skin. J Invest Dermatol. 1985 Jan;84(1):27–30. [PubMed]

Articles from The Journal of Clinical Investigation are provided here courtesy of American Society for Clinical Investigation