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Conceived and designed the experiments: HEA AK RCA SKW. Performed the experiments: HEA AK JW JR. Analyzed the data: HEA AK JW JR SKW. Contributed reagents/materials/analysis tools: JR. Wrote the paper: SKW.
HIRA (or Hir) proteins are conserved histone chaperones that function in multi-subunit complexes to mediate replication-independent nucleosome assembly. We have previously demonstrated that the Schizosaccharomyces pombe HIRA proteins, Hip1 and Slm9, form a complex with a TPR repeat protein called Hip3. Here we have identified a new subunit of this complex.
To identify proteins that interact with the HIRA complex, rapid affinity purifications of Slm9 were performed. Multiple components of the chaperonin containing TCP-1 complex (CCT) and the 19S subunit of the proteasome reproducibly co-purified with Slm9, suggesting that HIRA interacts with these complexes. Slm9 was also found to interact with a previously uncharacterised protein (SPBC947.08c), that we called Hip4. Hip4 contains a HRD domain which is a characteristic of the budding yeast and human HIRA/Hir-binding proteins, Hpc2 and UBN1. Co-precipitation experiments revealed that Hip4 is stably associated with all of the other components of the HIRA complex and deletion of hip4+ resulted in the characteristic phenotypes of cells lacking HIRA function, such as temperature sensitivity, an elongated cell morphology and hypersensitivity to the spindle poison, thiabendazole. Moreover, loss of Hip4 function alleviated the heterochromatic silencing of reporter genes located in the mating type locus and centromeres and was associated with increased levels of non-coding transcripts derived from centromeric repeat sequences. Hip4 was also found to be required for the distinct form of silencing that controls the expression of Tf2 LTR retrotransposons.
Overall, these results indicate that Hip4 is an integral component of the HIRA complex that is required for transcriptional silencing at multiple loci.
The assembly of specific regions of eukaryotic genomes into heterochromatin is important for processes such as gene silencing, nuclear organisation, development, dosage compensation and chromosome segregation. In the fission yeast, S. pombe the assembly of heterochromatin is directed by the RNAi machinery , , . Heterochromatic domains, such as those at centromeres, contain repeat sequences that are transcribed at low levels during S phase , . The resulting non-coding transcripts are processed to form siRNAs that are loaded into an RNAi effector complex called RITS , which directs the recruitment of a complex containing the histone H3 lysine 9 (H3K9) methyltransferase, Clr4 , , . In turn, methylated H3K9 is bound by chromodomain proteins such as the HP1 homolog, Swi6 . The integrity of heterochromatin at centromeres is also dependent upon the HIRA chromatin assembly factors, Hip1 and Slm9 . Mutation of either protein alleviates the silencing of marker genes located in centromeric outer repeats and leads to increased levels of chromosome missegregation .
HIRA (or Hir) proteins are evolutionarily conserved histone chaperones that mediate nucleosome assembly independently of DNA replication , , . Accordingly, human HIRA is found associated with histone H3.3, which is deposited into chromatin outside of S phase , . Roles for HIRA proteins in the assembly and maintenance of heterochromatin have been reported in a range of organisms. In S. cerevisiae the mutation of HIR genes does not alleviate silencing but does exacerbate the telomeric and HM silencing defects associated with inactivation of chromatin assembly factor 1 (CAF-1) , , . In S. pombe the loss of HIRA proteins results in silencing defects at centromeres and the mating type (mat) locus even in the presence of functional CAF-1 , . In human fibroblasts, HIRA drives the assembly of senescence associated heterochromatin in association with another histone chaperone, ASF1a ,  and in Arabidopsis HIRA is a component of a complex that maintains knox gene silencing in developing leaves , .
HIRA proteins execute their functions in the context of multi-subunit complexes. Human HIRA is associated with multiple proteins  and S. cerevisiae Hir1 and Hir2 are found to be stably associated with two structurally unrelated proteins, Hir3 and Hpc2 (also called Hir4) , . Similarly, the fission yeast HIRA proteins, Hip1 and Slm9, interact with Hip3, a homolog of S. cerevisiae Hir3 . In this study we report the identification and characterisation of an S. pombe Hpc2-related protein that we have called Hip4. Co-precipitation experiments indicate that Hip4 is stably associated with all of the subunits of the HIRA complex (Hip1, Slm9 and Hip3) and a hip4Δ strain has defects that are characteristic of cells lacking HIRA function. Deletion of hip4+ also alleviates heterochromatic transcriptional silencing at the mat locus and centromeres, and de-represses the expression of Tf2 LTR retrotransposons. Thus our data indicate that Hip4 is an integral component of the HIRA complex.
The fission yeast HIRA complex contains Hip1, Slm9 and a structurally unrelated protein called Hip3 . In order to identify additional components of the HIRA complex, and to identify other factors that interact with this complex, we employed a rapid affinity purification protocol that facilitates the isolation of large protein assemblies . Extracts from cells expressing a Slm9-FLAG fusion, were affinity purified and the resulting proteins were identified by liquid chromatography-tandem mass spectrometry. Numerous proteins were found to reproducibly co-purify with Slm9-FLAG (Table 1). Multiple components of the chaperonin containing TCP-1 complex (CCT, also called TRiC)  were present in Slm9-FLAG purifications. CCT is responsible for the folding of numerous proteins  and this suggests that it is required for the biogenesis of the HIRA complex. This analysis also suggests that HIRA interacts with the 19S regulatory particle of the proteasome  because multiple ATPase subunits (Rpt1, Rpt2, Rpt3, Rpt4 and Rpt5) from this complex co-purified with Slm9. Analysis of the list of Slm9-interacting proteins also revealed SPBC947.08c, a previously uncharacterised protein that we named Hip4 for HIRA interacting protein 4. Hip4 has limited homology to S. cerevisiae Hpc2 and human UBN1; Hpc2 is a component of the S. cerevisiae HIR complex ,  and during the course of this study Banumathy and co-workers reported that UBN1 functions in conjunction with human HIRA . Although the overall level of sequence identity of SPBC947.08c to both Hpc2 and UBN1 is low, it does contain a short sequence motif, termed an Hpc2-related domain (HRD), which is a characteristic of these proteins (Fig 1). In order to determine whether Hip4 also interacts with the other components of the HIRA complex its chromosomal locus was tagged with the TAP epitope (hip4-TAP). Whole cell extracts from hip4-TAP cells were partially purified using IgG magnetic beads and analysed by western blotting. This confirmed that Slm9 co-purifies with Hip4 (Fig 2A) and furthermore, similar experiments revealed that both Hip1 and Hip3 co-precipitate with Hip4 (Fig 2B & C). Therefore, Hip4 is stably associated with all the components of the S. pombe HIRA complex.
In order to investigate the function of this protein we constructed a hip4Δ strain. Cells lacking Hip4 were found to be viable but exhibited a number of phenotypes that are reminiscent of cells lacking the function of the HIRA complex (Fig 3). Like other HIRA mutants, the hip4Δ strain was temperature sensitive showing a limited ability to proliferate at 37°C (Fig 3A). Furthermore, microscopic examination of hip4Δ cells revealed that they had an elongated phenotype similar to hip1Δ, slm9Δ and hip3Δ cells (Fig 3B), which are known to have a G2 cell cycle delay , , . The introduction of a plasmid ectopically expressing hip4 (pRep41Pk-Hip4) rescued the elongated cell phenotype of hip4Δ cells (Fig 3C) confirming that this defect is due to lack of Hip4 function.
The HIRA complex is required for accurate chromosome segregation and consistent with this hip1Δ, slm9Δ and hip3Δ cells exhibit hypersensitivity to thiabendazole (TBZ), a drug which depolymerises microtubules and thus impairs the function of the mitotic spindle , . Deletion of hip4+ also resulted in a marked increase in TBZ sensitivity, suggesting that Hip4 is also required for accurate chromosome segregation (Fig 3D). Increased sensitivity to TBZ is often correlated with dysfunctional centromeric heterochromatin. In fission yeast, centromeric heterochromatin is assembled onto arrays of dg-dh repeats that flank a central core domain . Marker genes inserted into dg-dh repeats are subjected to strong transcriptional silencing and so cells carrying the ade6+ gene in this region form dark red colonies when adenine is limiting  (Fig 4A). Deletion of hip1+, slm9+ or hip3+ results in reduced trans-gene silencing and the formation of pink colonies , . Similarly, introduction of the hip4Δ allele into this background resulted in the formation of pink colonies indicating that Hip4 is also required for reporter gene silencing at dg-dh repeats (Fig 4A).
Paradoxically, the proper assembly of ‘silent’ centromeric heterochromatin requires transcription of the dg-dh repeats from both strands , , . These non-coding transcripts are processed by the RNAi machinery to form siRNAs which play an important role in the assembly of heterochromatin at the centromere. Transcription the dg-dh repeats is differentially regulated: the reverse strand (cenREV) is transcribed in wild type cells at low levels and rapidly processed by the RNAi machinery whereas transcription in the forward direction (cenFOR) is limited by heterochromatin . Accordingly, mutations that impair heterochromatin function often result in increased levels of cenFOR transcripts. Quantitative RT-PCR analysis revealed that transcripts from dg-dh repeats were increased (1.59 fold [±0.1]) in a slm9Δ background. We therefore used strand-specific RT-PCR to further analyse dg-dh transcripts and found that increased levels of cenFOR transcripts were detectable in hip1Δ, slm9Δ, hip3Δ and hip4Δ cells (Fig 4B). This is consistent with a role for the HIRA complex in the function of pericentric heterochromatin.
Heterochromatin is also found at the mat locus, although at this region the assembly of heterochromatin is only partially dependent upon the RNAi machinery . Marker genes that are inserted into the mat region are subjected to strong transcriptional silencing that is dependent upon the HIRA complex ,  (see also Fig 4C). Deletion of hip4+ also abolished the silencing of an ade6+ reporter located at mat3-M (Fig 4C). This indicates that the function of the HIRA complex at heterochromatin requires the Hip4 subunit.
The genome of the sequenced S. pombe strain (972), harbours 13 full-length copies of the Tf2 LTR retrotransposon . These elements are silenced by a mechanism that is distinct from the mat locus and centromeres being independent of H3K9 methylation , . Instead the transcriptional silencing of these elements requires CENP-B homologues  and multiple histone deacetylases (Clr6, Clr3 and Hst4) , , . It is also dependent upon the function of the HIRA complex as global Tf2 mRNA levels are dramatically increased in hip1Δ, slm9Δ and hip3Δ backgrounds . Furthermore, northern blotting and quantitative RT-PCR analyses revealed that Tf2 mRNA levels were similarly increased in hip4Δ cells (Fig 5A & B), indicating that LTR retrotransposon silencing requires Hip4. Taken together our data indicate that Hip4 is an integral component of the HIRA complex that is required for silencing at multiple loci.
Here we have identified a new component of the S. pombe HIRA complex. Co-precipitation analyses indicate that Hip4 is stably associated with Hip1, Slm9 and Hip3. Furthermore, deletion of hip4+ results in phenotypes that are characteristic of cells lacking HIRA complex function. Hip4 is necessary for the integrity of heterochromatin at centromeres and at the mat locus and is also required for the distinct form of silencing that represses the expression of Tf2 LTR retrotransposons.
Hip4 appears to be the fission yeast counterpart of S. cerevisiae Hpc2 and human UBN1, however the sequence similarity between these proteins is essentially restricted to the HRD domain (also called HUN, for Hpc2-Ubinuclein-1 domain) . The HRD domain of UBN1 binds to the N-terminal WD repeats of HIRA and similarly, the HRD domain is required for the interaction of Hpc2 with S. cerevisiae Hir1 . Furthermore, a human HIRA mutant that harbours an arginine to lysine substitution in amino acid 227, between the fourth and fifth WD repeats, is unable to bind to UBN1 . This critical arginine residue is conserved in S. pombe Hip1, and indeed the whole WD repeat region of Hip1 and human HIRA exhibit considerable sequence similarity . It is therefore highly likely that Hip4 directly interacts with the WD repeats of Hip1. However, Slm9 also contains recognisable WD repeat motifs in its N-terminal region and so a direct interaction between Hip4 and Slm9 cannot be discounted.
The identification of Hip4 indicates that the composition of the S. pombe HIRA complex is highly similar to that of budding yeast, in which the HIRA/Hir subunits interact with two structurally unrelated proteins (Hir3 and Hpc2 in S. cerevisiae; Hip3 and Hip4 in S. pombe). Moreover, recent evidence indicates that this fundamental degree of similarity extends to human HIRA complexes. Following the identification of UBN1 as an ortholog of Hpc2 , recent bioinformatic analyses have suggested that the human counterpart of Hir3/Hip3 is the calcineurin binding protein, Cabin1 . Importantly, both UBN1 and Cabin1 are known to be associated with HIRA because a histone H3.3 complex was found to be composed of HIRA, UBN1, Cabin and another histone chaperone ASF1a . Human HIRA interacts with ASF1a through its B domain, which is located in central portion of the protein  and an interaction between S. cerevisiae Hir1 and Asf1 has also been demonstrated . While affinity purifications of fission yeast HIRA proteins have not identified the S. pombe Asf1 homologue (Cia1), an interaction between this protein and the B-domain from Hip1 has been demonstrated in vitro . Thus the core interactions of the budding yeast, fission yeast and human HIRA/Hir proteins appear to be highly conserved (Fig 6).
The affinity purification experiments also indicate an interaction between the HIRA complex and the proteasome and so it is possible that HIRA subunit levels may be regulated by proteasomal degradation. However, alternative explanations for this interaction are possible given that Slm9 co-purifies with 19S ATPase subunits that have previously been linked to chromatin and transcriptional regulation. Indeed, it has been proposed that 19S ATPase subunits can exist independently of the proteasome and exert non-proteolytic functions in transcriptional control . In S. cerevisiae the 19S ATPases, Sug1 and Sug2, which are counterparts of S. pombe Rpt6 and Rpt4, are recruited to the promoters of specific genes (HSP26, HSP104 and GAD1) and are required for their induction in response to stress . Similarly, in HeLa cells 19S ATPase subunits regulate chromatin modifications and expression at the CTIIA and MHC-II promoters . In both cases the function of the proteasomal subunits is independent of proteolysis , . Furthermore, others have shown that the proteasome co-operates with the histone chaperone FACT in the disassembly of nucleosomes at PHO5 during transcriptional induction . It is therefore possible that 19S ATPase subunits antagonise the repressive functions of the HIRA complex.
Slm9 affinity purifications also revealed interactions with subunits of the chaperonin CCT, which is known to mediate the folding of numerous proteins . This suggests that the biogenesis of HIRA complexes may be CCT-dependent. In support of this hypothesis, analysis of CCT substrates in S. cerevisiae has revealed several chromatin remodelling complexes and furthermore CCT is known to have a high affinity for the β-propeller fold adopted by WD repeats . The functional relationship between HIRA and many other Slm9 co-purifying proteins is obscure. For example, enzymes involved in nucleotide and amino acid metabolism (Ura1, Cts1, Lys4 Ade1 and Anc1) and also tRNA synthetases (Vas2, Ths1 and SPAC17A5.15c) were reproducibly found in Slm9-immunoprecipitates. However, an interaction between HIRA and CCT may go some way to explaining the presence of these unexpected proteins. The CCT interaction network in S. cerevisiae is known to include numerous enzymes  and therefore, many proteins found in Slm9 pull-downs may be present by virtue of being CCT substrates.
Our data indicate that Hip4, like all other components of the HIRA complex, is required for heterochromatic silencing. Similarly, UBN1 is required for the HIRA/ASF1a pathway that establishes domains of senescence associated heterochromatin in senescent fibroblasts . However, the functions that the HRD domain proteins fulfil within HIRA/Hir complexes are not understood. Although, it has been proposed that HRD domain proteins function as histone chaperones that bind to histone tails , there is as yet no direct evidence to support this. Interestingly, analysis of S. cerevisiae Hpc2 has revealed that it exhibits genetic interactions that are not observed with Hir1, Hir2 and Hir3 , , suggesting that it has functions that are independent of the Hir complex. Given that it is now clear that HRD domain proteins have been conserved throughout evolution , , it will be important to determine the precise roles of these proteins in the organization of chromatin structure.
The strains used in this study are described in Table 2. The hip4+ ORF was disrupted using one-step gene replacement. A fragment from the 5′ end of the hip4+ ORF was PCR amplified using oligonucleotides Hip4KOA (5′-GATGCTCTTCTTGTTCGTACTCGTTTCAAAG -3′) and Hip4KOB (5′-CAGTATCTCCTTAAGCTTGACGAACAAAAGCGAAGTATAG-3′) and a fragment from the 3′ end of the hip4+ ORF was PCR amplified using oligonucleotides Hip4KOC (5′-TTTGTTGCTAAGCTTAAGGAGATACTGTGAAAATCG-3′) and Hip4KOD (5′-ATCGATTGACAGATCTTCAGG-3′). These fragments were used as the template in an overlapping PCR reaction with oligonucleotides primers Hip4KOA and Hip4KOD. The resulting fragment was cloned into pGEM-T (Promega) to yield pGEM-Hip4 and the 1.8 kb ura4+ cassette from pRep42 was then cloned into the HindIII site to give plasmid pGEM-Hip4::ura4+. The PstI-BglII fragment from pGEM-Hip4::ura4+ was used to transform a diploid strain and integration at the correct locus was confirmed by PCR. The genomic locus of hip4+ was tagged with the TAP epitope by PCR amplifying a fragment using the oligonucleotides primers hip4Pk-PstI (5′-GTTCACCTGCAGGAAGGAGATACTGTGAAAATC-3′) and hip4Pk-BamHI (5′-CAGTTCGGATCCTGGGATGCTACTAGTTGGAAT-3′). The fragment was digested with BamHI and PstI and cloned into the PstI and BamHI sites of pRip42-CTAP . The resulting plasmid was linearised with BstXI and transformed into the appropriate strains. The pRep41Pk-Hip4 plasmid was constructed by PCR amplifying the hip4+ ORF using oligonucleotide primers hip4NdeI (5′-GTCACGCATATGTCGCTTTGTCTGGCGAC -3′) and hip4Pk-BamHI, digesting the resulting DNA fragment with BamHI and NdeI and cloning it into pRep41PkN . The genomic locus of slm9+ was tagged with three copies of the FLAG epitope (3XFLAG) by PCR amplifying a fragment from p3X-FLAG-CMV (Sigma) using oligonucleotides 3XFLAG3′ (5′-GCTAGCTCCATGGATCACTACTTGTCATCGTC-3′) and 3XFLAG5′ (5′-CGTAGGCGGATCCCCGGGTGACTACAAAGACCATGACGGT-3′). The resulting fragment was digested with BamHI and NcoI and cloned into the BamHI and NcoI sites of pRip42-Slm9-CTAP to give plasmid pRip42-Slm9-3XFLAG. The resulting plasmid was digested with XbaI and transformed into wild type cells.
Immunoaffinity purifications for LC-MS/MS analysis were performed as described , with the following modifications. Purifications were performed on extracts derived from 5 g of cells using Dynabeads coupled to anti-FLAG M2 antibody (Sigma, F1804) for 15 mins. The immunoprecipitated material was treated with 500 U of Benzonase, washed, subjected to on-bead tryptic digestion, and prepared for LC-MS/MS analysis as described previously . Briefly, immunoprecipitated material was subjected to on-bead Tryptic digestion. The resulting peptides were desalted using StageTips ,  and analyzed using LC-MS on a LTQ-Orbitrap (Thermo Fisher Scientific) coupled to HPLC via a nanoelectrospray ion source. The six most intense ions of a full MS acquired in the orbitrap analyzer were fragmented and analyzed in the linear ion trap. The MS data were analyzed using MaxQuant  and proteins identified by searching MS and MS/MS data using the MASCOT search engine (Matrix Science, UK). Table 1 lists proteins identified in at least two independent purifications from cells expressing the Slm9-FLAG fusion and absent from four independent control purifications from untagged cells. Ribosomal proteins (common contaminants) were also excluded.
Whole cell extracts were prepared as described previously except that the standard lysis buffer was substituted with HB buffer (25mM Tris-HCl [pH 7.5], 15 mM EGTA, 15 mM MgCl2, 0.1% NP40, 1 mM DTT, 0.1 mM NaF). Immunoprecipitations were performed by adding 25 µL IgG-coupled Dynal beads to 1 mg of whole protein extract and incubating for 1 hour at 4°C with gentle agitation. Beads were recovered and washed three times with 1 ml HB buffer. Samples were electrophoresed through SDS-PAGE gels and subjected to western blotting using monoclonal anti-FLAG antibodies (Sigma), monoclonal anti-Pk (Serotec) and Peroxidase Anti-Peroxidase soluble complex produced in rabbit (Sigma).
RNA was purified and analysed as described previously . Gene specific probes were produced by PCR amplification from genomic DNA using the appropriate primers. All probes were labelled with α-[32P]-dCTP using a Prime-a-Gene labelling kit (Promega).
For strand-specific RT-PCR, RNA was purified as previously described  and subjected to RT-PCR using a One Step RT-PCR kit (Qiagen). One primer complementary to the sense or antisense transcript was added during first strand cDNA synthesis while the second primer was added prior to the PCR amplification steps. cDNA for quantitative (real-time) RT-PCR was made using a Superscript II kit (Invitrogen). Real-time PCR reactions were performed using a LightCycler 2.0 PCR system (Roche) and SYBR Green mix (Molecular Probes) using the appropriate primers . Reactions were normalised using primers specific to act1+. Data for quantitative (real-time) RT-PCR are the mean of three experiments. Error bars indicate ± SD.
We thank Elizabeth Veal and Heather Murton for their comments on the manuscript, Flavia de Lima Alves for help with the mass spectrometric analysis and Ricardo Almeida for bioinformatics support.
Competing Interests: The authors have declared that no competing interests exist.
Funding: This work was supported by a Biotechnology and Biological Sciences Research Council (BBSRC) Doctoral Training Grant to H.E.A, a BBSRC Project Grant (BB/E014445/1) to S.K.W., a Medical Research Council Strategic grant (G0301153) to R.C.A., an EC Marie-Curie Excellence grant (MEXT-CT-014171) to J.R, a Wellcome Trust Senior Research fellowship (084229/Z/07/Z) to J.R. and a Wellcome Trust Programme grant (065061/Z) to R.C.A. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.