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Medical Microbiology and Immunology
 
Med Microbiol Immunol. 2010 November; 199(4): 299–309.
Published online 2010 May 8. doi:  10.1007/s00430-010-0163-0
PMCID: PMC2955911

Pro-autophagic signal induction by bacterial pore-forming toxins

Abstract

Pore-forming toxins (PFT) comprise a large, structurally heterogeneous group of bacterial protein toxins. Nucleated target cells mount complex responses which allow them to survive moderate membrane damage by PFT. Autophagy has recently been implicated in responses to various PFT, but how this process is triggered is not known, and the significance of the phenomenon is not understood. Here, we show that S. aureus α-toxin, Vibrio cholerae cytolysin, streptolysin O and E. coli haemolysin activate two pathways leading to autophagy. The first pathway is triggered via AMP-activated protein kinase (AMPK). AMPK is a major energy sensor which induces autophagy by inhibiting the target of rapamycin complex 1 (TORC1) in response to a drop of the cellular ATP/AMP-ratio, as is also observed in response to membrane perforation. The second pathway is activated by the conserved eIF2α-kinase GCN2, which causes global translational arrest and promotes autophagy in response to starvation. The latter could be accounted for by impaired amino acid transport into target cells. Notably, PKR, an eIF2α-kinase which has been implicated in autophagy induction during viral infection, was also activated upon membrane perforation, and evidence was obtained that phosphorylation of eIF2α is required for the accumulation of autophagosomes in α-toxin-treated cells. Treatment with 3-methyl-adenine inhibited autophagy and disrupted the ability of cells to recover from sublethal attack by S. aureus α-toxin. We propose that PFT induce pro-autophagic signals through membrane perforation–dependent nutrient and energy depletion, and that an important function of autophagy in this context is to maintain metabolic homoeostasis.

Keywords: Autophagy, Pore-forming toxins, Starvation, AMPK, eIF2α-kinases, S. aureus

Introduction

Pore-forming toxins (PFT) are important virulence factors of many pathogenic bacteria [14], and there is a growing interest in innate defense mechanisms against PFT [4, 5]. Various signaling pathways have been implicated in the survival of target cells [611], but both sensor and effector mechanisms remain incompletely understood. Based on the observation that target cells of PFT may recover from a substantial drop of cellular ATP, we have advanced the concept that the repair of membrane lesions is an important aspect of cellular defense against these toxins [1214]. Following the discovery by Huffman et al. that p38 MAPK is important for the survival of C. elegans exposed to PFT-producing bacteria or of mammalian cells incubated with aerolysin [6], we found that p38 is required at an early stage after sublethal attack by S. aureus α-toxin to promote metabolic recovery of the target cell. Curiously, p38 was not required for recovery from attack by streptolysin O (SLO), which forms much larger membrane pores, leading to the proposal that membrane repair is diverse [7]. Consistently, membrane lesions formed by streptolysin O (SLO) are reportedly internalized by a dynamin-independent mechanism [15], whereas those caused by α-toxin are eliminated by dynamin-dependent endocytosis and subsequent exocytosis [16]. Although these findings provide potential explanations for the reconstitution of membrane integrity, the mechanisms involved in maintaining metabolic homoeostasis during transient membrane perforation remain to be investigated.

Recent work on Vibrio cholerae cytolysin (VCC) indicated that autophagy may play a role in the cellular defense against this toxin [17]. Autophagy is a disposal and recycling pathway in cells [18], which is characterized by the formation of autophagosomes, structures limited by two membrane-bilayers, enclosing endogenous or exogenous material, including aggregated molecules, damaged organelles, invading organisms or cytoplasm. Genetic approaches in yeast have identified some 30 genes that are involved in autophagy, and mammalian homologues have been identified for many of them. The mammalian protein LC3 (Atg8 in yeast) is thought to play an important role during autophagosome formation. Proteolytic cleavage of a precursor generates LC3-I, which is then conjugated at its C-terminal glycine with phosphatidylethanolamine, yielding LC3-II. The lipidated protein associates with the phagophorous membrane and promotes autophagosome formation, a process underlying complex regulation [19].

A major regulator of autophagy is the target of rapamycin (TOR), which integrates multiple signaling pathways involved in metabolic regulation [20]. TORC1 (TOR-complex 1) is activated under conditions that favour growth. If growth factors, nutrients and ATP are abundant, p70S6 K, a downstream-target of TORC1, is phosphorylated, promoting translation via phosphorylation of the ribosomal protein S6 and the eIF4E-binding protein 4E-BP. Conversely, starvation and energy depletion and several other triggers result in the dephosphorylation of S6 K and 4E-BP, translational arrest and induction of autophagy; for review, see [2123]. For example, lowering of the cellular ATP/AMP ratio triggers phosphorylation of AMP-activated protein kinase (AMPK). Phospho-AMPK inhibits TORC1, leading to dephosphorylation of S6 K, reduced translation and enhanced autophagy.

Autophagy induction upon starvation or viral infection depends on a second pathway, which is triggered by eIF2α-kinases GCN2 and PKR, respectively [24]. Although the mechanisms of autophagy-induction via eIF2α-phosphorylation are not fully understood, crosstalk with the mTOR pathway may play a role. GCN2 (general control, non-derepressible), also termed eIF2α-kinase 4 (EIF2AK4), functions as a nutrient sensor. It is the only known eIF2α-kinase conserved from yeast to mammals, and is basically triggered by uncharged tRNAs. Three additional eIF2α-kinases have been identified in mammalian cells, which are activated by diverse types of stress [25, 26]: HRI (EIF2AK1) responds to heme deficiency, PKR (EIF2AK2) confers eIF2α phosphorylation in response to double-stranded RNA, and PERK (EIF2AK3) is a sensor of ER-stress. Phosphorylation of eIF2α causes global translational arrest; some genes are exempt from this process and instead become overexpressed [27, 28]. This so-called integrated stress response (ISR) is thought to prevent the accumulation of unfolded proteins during stress, to conserve energy and to reprogram gene expression. EIF2α phosphorylation is critically involved in both innate and adaptive immunity [2933]. It is also important for the downregulation of protein synthesis during hibernation [34] and for the formation of memory [35, 36].

How PFT trigger autophagy is not known. Regarding the function of autophagy, it was speculated that destruction of toxin was responsible for the pro-survival effect in target cells of VCC [37]. However, because autophagy is also of great importance for nutrient and energy homoeostasis [19], and because cellular recovery from an attack by S. aureus α-toxin appears not to involve destruction of pore complexes [19], we questioned that the model proposed for VCC applies to α-toxin. In the present work, we show that diverse PFT trigger sensors of energy depletion and starvation, which are established activators of autophagy. Thus, PFT trigger AMPK and the ISR, and evidence is presented that membrane perforation and dissipation of ion gradients play a critical role for activation of these pathways. Further, the possibility is raised that the amelioration of acute starvation and energy crisis are important functions of autophagy during cellular defense against S. aureus α-toxin.

Materials and methods

Cells and media

HaCaT is a non-virally transformed human keratinocyte cell line, kindly provided by Prof. Dr. N. Fusenig (Deutsches Krebsforschungsinstitut (DKFZ), Heidelberg, Germany) [38]. Cos7 cells were from Deutsche Sammlung für Mikroorganismen und Zellen (DMSZ), Braunschweig, Germany, and CD14-deficient human bladder carcinoma cell line A498 was from Cell Lines Services (CLS), Heidelberg. Cells were cultured in DMEM with 10% fetal calf serum (FCS) in a humidified incubator with 5% CO2. Experiments to evaluate the impact of potassium efflux in the activation of GCN2 were performed with custom-made potassium[K+]—hi medium containing 80 mM K+ and correspondingly lower Na+ (PAN Biotech GmbH); normal medium is termed [K+]—n in the text.

Reagents

Wild-type α-toxin, single amino acid exchange mutant D152C, streptolysin O (SLO), Vibrio cholerae cytolysin (VCC) and E. coli haemolysin A (HlyA) were prepared, and the haemolytic activity was measured as described [3942]. AlexaFluor546-labelling of α-toxin was performed with reagents from Invitrogen.

Rabbit polyclonal antibodies against GCN2, phosphorylated GCN2 (p-GCN2), eIF2α phosphorylated at serine 51 (p-eIF2α), eIF2α, phospho-p70S6 kinase (p-p70S6 K), p70S6 K, phospho-AMPKα (Thr172), AMPKα and horseradish peroxidase (HRP)–conjugated goat anti-rabbit IgG were purchased from Cell Signaling Technology. Rabbit antibody against GFP and mouse monoclonal anti-p62/SQSTM1 were from Abcam, mouse antibody against β-actin was from Sigma, horseradish peroxidase (HRP)–conjugated goat anti-mouse IgG was from Santa Cruz Biotechnology INC. The LC3-antibody was from Nanotools. Nigericin, monensin, 3-methyladenine (3-MA) and bafilomycin were purchased from Merck Biosciences, Schwalbach, Germany. The N-term-GADD34-expression plasmid [28] was obtained from Addgene.

Western blots

Subconfluent cell monolayers grown in 6-well plates were washed once in PBS and solubilized in situ by the addition of 80 μl of 2× SDS-loading buffer (10%(v/v) glycerol, 5%(v/v) 2-ME, 2%(w/v) SDS and bromophenol blue). Western blots for the detection of phosphorylated and total AMPK, p70S6 K and GFP were performed as published elsewhere [43]. For the detection of p-GCN2, separation of proteins by SDS–PAGE (7.5%) was followed by electroblotting onto nitrocellulose at 100 mA for 80 min, a subsequent wash in PBS, blocking for 1 h at RT in 5% BSA (w/v) in PBS, two brief washing steps in PBS, incubation with first antibody (rabbit-anti-pGCN2) over night at 4°C, three washing steps in PBS, 0.02% Tween 20 and incubation with goat anti-rabbit IgG HRP conjugate (1:1,000) in PBS with 5% BSA, for 1 h at RT. The membrane was washed three times in PBS containing 0.02% Tween 20, and bound antibody was detected by ECL (Roche).

3H-Leucine uptake

HaCaT cells (2 × 105 per well) were seeded into 24-well culture dishes 1 day prior to treatment with α-toxin (500 ng/ml) in complete medium for 15 min at 37°C. Subsequently, toxin-containing medium was withdrawn, and cells were washed with PBS before 500 μl of medium devoid of cold leucine, but supplemented with 7.5 μCi per well of L-[4, 5-3H]-leucine (GE Healthcare, Freiburg, Germany), was added. Cells were incubated for 1, 2 or 3 min at 37°C, washed three times with ice-cold PBS, lysed in 200 μl of 0.1 N NaOH and lysates were analysed in a liquid scintillation counter (Beckman).

PKR phosphorylation assay

Because phospho-PKR (p-PKR) antibodies yielded poor results, we employed an assay based on the incorporation of radioactive phosphate and precipitation of p-PKR with double-stranded RNA-coated beads [44]. HaCaT cells (5 × 105) were seeded into T25-flasks 1 day before treatment with α-toxin or D152C as indicated. Cells were washed with ice-cold PBS and lysed in lysis buffer (20 mM HEPES pH 7.5, 120 mM KCl, 5 mM MgCl2, 1 mM DTT, 0.5% NP40, 2 μg/ml leupeptin, 50 μg/ml aprotinin, 25 U/ml RNase inhibitor) for 10 min on ice. Lysates were centrifuged and 200 μg protein/supernatant were mixed with 50 μl reaction buffer (20 mM HEPES pH 7.5, 120 mM KCl, 5 mM MgCl2, 1 mM DTT, 10% glycerol) and 50 μl ATP mixture (6 μCi γ-32P-ATP, 50 μM cold ATP in reaction buffer). After 15 min at 30°C, polyI:C beads were added and incubated for 1 h at 4°C with agitation. Beads were washed four times with wash buffer (20 mM HEPES pH 7.5, 90 mM KCl, 300 mM NaCl, 0,1 mM EDTA, 10% glycerol) and boiled for 5 min in 50 μl 2× SDS-loading buffer. After centrifugation, proteins were separated by SDS–PAGE (10%), and labelled proteins were revealed by fluorography using Amplify, FUJI super RX films and a Kodak amplifier screen.

Immunofluorescence microscopy

HaCaT cells were seeded on glass cover slips 1 day before transient transfection of EGFP-LC3 plasmid (kindly provided by Prof. Yoshimori, Research Institute for Microbial Diseases, Osaka) with jetPEI™ (Polyplus-transfection SA, France) as recommended by the supplier. After 24 h, cells were treated as described in legends, washed with PBS, fixed with 2% paraformaldehyde for 10 min at room temperature, washed three times with PBS and embedded in Fluoroprep (bioMérieux® SA, France). Immunofluorescence microscopy was performed with a ZEISS Axiovert 200 M microscope, using a Plan-Apochromat 100×/1.4 oil objective; digital images were acquired with a ZEISS AxioCam and processed with Axiovision software and Adobe photoshop. EGFP-LC3-positive puncta per cell, (for n > 30), was enumerated using ImageJ software (NIH).

Electron microscopy

The procedure used for electron microscopy (EM) has been published elsewhere [45]. In brief, cells were fixed with 2.5% glutaraldehyde in PBS for 45 min at room temperature and embedded in Epon 812 according to standard protocols. Seventy nanometre ultrathin sections were cut, stained with 1% lead citrate and 2% uranyl acetate and finally analysed in Zeiss EM 902 electron microscope, equipped with TRS digital camera.

ATP measurements

HaCaT cells were seeded at a density of 2 × 104 per well into 96-well tissue culture plates, and cellular ATP was analysed with the Bioluminescence Assay Kit CLS II (Roche), using a Lumat 9705 instrument (Berthold Technologies, Bad Wildbad) as described [46].

Replicates and statistical analysis

All data are representative of at least two independent experiments. Two-tailed unpaired Student’s t-test was employed to assess statistical significance of differences between mean values (Fig. (Fig.7c).7c). Significance was assumed at P ≤ 0.05.

Fig. 7
a HaCaT cells were treated for 4 h with 200 ng/ml α-toxin or mutant toxin in the absence or presence of 3-MA, as indicated in the figure. The presence of EGFP-LC3-II was detected by Western blot. b HaCaT cells were treated with ...

Results

Transient response of AMPK and S6 K to sublethal membrane perforation by S. aureus α-toxin

AMP-activated protein kinase (AMPK) is an obvious candidate mediator of autophagy in target cells of PFT, because it is activated upon lowering of the cellular ATP/AMP ratio and because PFT cause energy depletion [1214, 46]. Western blots of lysates from α-toxin-treated HaCaT cells revealed transient phosphorylation of AMPK under conditions of reversible ATP loss (Fig. 1a, b), whereby the time of p-AMPK-dephosphorylation correlated with ensuing ATP replenishment.

Fig. 1
Transient ATP loss is accompanied by transient AMPK phosphorylation and dephosphorylation of S6K. HaCaT cells were left untreated (Co) or loaded with α-toxin (1 μg/ml) for 40 min on ice, washed with ice-cold PBS and incubated ...

Next, we analysed the phosphorylation status of p70S6K in cells treated with S. aureus α-toxin. As depicted in Fig. 1c, p70S6K became dephosphorylated upon treatment with α-toxin. This effect too was reversible within hours, in keeping with the notion that the treatment conditions chosen here led to sublethal, transient perforation of cells.

Diverse PFT activate AMPK

As expected from the fact that VCC, SLO and HlyA also cause a drop of cellular ATP levels, they too activated AMPK phosphorylation (Fig. 2a–c). As [K+]—hi media markedly reduced this effect, as shown here for SLO (Fig. 2b), efflux of potassium may be an upstream trigger of AMPK in response to PFT.

Fig. 2
Activation of AMPK by various PFT. a HaCaT cells were incubated with 100 ng/ml VCC for indicated times, and the phosphorylation status of AMPK was analysed by Western blot. b HaCaT cells were treated with 1 μg/ml SLO in [K+]-n ...

S. aureus α-toxin reduces the uptake of leucine

Because solute transport into cells depends on ion gradients [47], we reasoned that PFT might—apart from depleting cellular energy stores—also cause shortage of nutrients. Therefore, we measured net transport of radiolabelled leucine into α-toxin-treated cells and found that accumulation of the amino acid was indeed retarded (Fig. 3a). Similar results were obtained with SLO (data not shown) suggesting that membrane pores of different size, structure and lifetime can reduce active nutrient transport into cells.

Fig. 3
aS. aureus α-toxin inhibits leucine uptake. Accumulation of tritiated l-leucine was measured in HaCaT cells that were pre-treated with α-toxin (100 ng/ml) for ten minutes. Columns indicate mean values of quadruplicate measurements; ...

Membrane pore formation causes phosphorylation of GCN2 and PKR

Autophagy in response to lack of amino acids is regulated by GCN2 and downstream phosphorylation of eIF2α at Ser51 [24]. GCN2 is activated by the binding of uncharged tRNAs, which leads to autophosphorylation of threonine residues within its activation loop. Western blots using antibodies against phospho-GCN2 revealed rapid and transient phosphorylation upon α-toxin treatment whereby high concentrations of potassium in the extracellular milieu selectively blocked activation (Fig. 3b). A single amino acid exchange mutant, D152C, which binds to cells and assembles into heptamers, but is inefficient in forming trans-membrane pores [48], caused much less GCN2 phosphorylation (Fig. 3c).

Like α-toxin, VCC, SLO and HlyA also activated GCN2 (Fig. 3d–f). Because there is evidence that potassium efflux is the common trigger of MAPK p38 in cells perforated by various PFT [49], we reasoned that it might also trigger GCN2 phosphorylation. As shown in Fig 3g, nigericine, and to a lesser extent monensin, activated GCN2. Therefore, we conclude that disrupted cellular homoeostasis of the major monovalent cations leads to GCN2 phosphorylation.

Next, we sought to test whether in addition to GCN2 (EIF2AK4) other eIF2α-kinases are activated by α-toxin. In line with results from C. elegans [9], phosphorylation of PERK (EIF2AK3) could not be discerned in α-toxin-treated cells (data not shown). However, phosphorylation of the double-stranded RNA-binding kinase, PKR (EIF2AK2), was detectable by employing an assay based on 32P-ATP-incorporation, pull-down of polyI:C-binding proteins, separation by SDS–PAGE and subsequent autoradiography (Fig. 4a). Pull-down with poly-C beads served as a control. Neither polyI:C, an activator of PKR in monocytes, nor TNFα, H2O2 or nigericine had a comparable effect as α-toxin, suggesting that membrane perforation is a unique trigger of PKR in HaCaT cells. Thus, at least two eIF2α-kinases are activated by α-toxin, both of which have been implicated in the induction of autophagy [19] and references therein.

Fig. 4
a Activation of PKR by α-toxin. Upper panel: HaCaT cells were treated with α-toxin and phosphorylation of a double-stranded RNA-binding protein of apparent MW 75kD (referred to as PKR) was determined. Cells were incubated with the indicated ...

Consistent with the above observations, eIF2α became phosphorylated in response to PFT, which is exemplified here by the Western blot shown in Fig. 4b, where the response to S. aureus α-toxin is shown.

Accumulation of autophagosomes upon attack by S. aureus α-toxin depends on pore formation and eIF2α-phosphorylation, and is sensitive to 3-MA

Transmission-EM of α-toxin-treated cells revealed many vacuoles, multivesicular bodies (MVB) and structures suggestive of autophagosomes or derivatives thereof (Fig. 5a).

Fig. 5
a TEM images of HaCaT cells. Left panel: untreated, size bar 1 μm; right: α-toxin for 2 h, size bar 500 nm. b Digital fluorescence microscopic image of HaCaT cells treated with AlexaFluor546-labelled α-toxin ...

Furthermore, double fluorescence experiments demonstrated that endocytosed AlexaFluor546-α-toxin colocalized with EGFP-LC3-containing puncta (Fig. 5b). Similarly, labelled α-toxin colocalized with p62/SQSMT1 (sequestosome1), a protein that serves as a linker between LC3 and ubiquitinated cytoplasmic material destined to be autophagocytosed (Fig. 5b). Toxin-negative vesicles containing p62 or LC3 were also more abundant in AlexaFluor546-α-toxin-treated cells. Although the number of EGFP-LC3-puncta was markedly enhanced by incubating cells with α-toxin (200 ng/ml), mutant D152C did not exert a significant effect at this concentration (Fig. 6a), suggesting that accumulation of autophagosomes in response to α-toxin requires membrane pore formation. Accumulation of LC3-positive puncta in toxin-treated cells was confirmed using an antibody against endogenous LC3-II (data not shown). The effect of wild-type α-toxin was reduced by 3-MA (Fig. 6a), suggesting that under the present conditions canonical pathways contributed to the accumulation of autophagy.

Fig. 6
a HaCaT cells were transfected with EGFP-LC3 and treated with 200 ng/ml wild type (α-Tox), or D152C mutant (MUT) for 4 h. (Co) denotes untreated sample and (α-Tox + 3-MA) a sample pretreated with 3-MA (10 mM) ...

GCN2 and PKR are eIF2α-kinases known to activate autophagy in response to starvation and viral infection, respectively. Given that α-toxin activated both kinases, it appeared likely that phosphorylation of eIF2α played a role for the accumulation of autophagosomes in α-toxin-treated cells. If this was indeed the case, GADD34, a subunit of protein-phosphatase 1 that targets the catalytic subunit (pp1c) to eIF2α (and thereby promotes eIF2α-dephosphorylation), should inhibit the effect of α-toxin. This assumption was further fuelled by our earlier observation by serial analysis of gene expression (SAGE) that GADD34 transcript levels are induced in α-toxin-treated HaCaT cells [46]. We cotransfected HaCaT cells with GADD34 and EGFP-LC3, and treated them with α-toxin. Overexpression of GADD34 suppressed α-toxin-dependent accumulation of EGFP-LC3-positive puncta (Fig 6b), whereas salubrinal, an inhibitor of GADD34, enhanced the number of autophagosomes even in the face of GADD34 overexpression (13 puncta per cell (median) in GADD34-transfected cells receiving α-toxin only vs. 30 puncta in cells receiving α-toxin plus salubrinal). EGFP-LC3 was also recruited to α-toxin-producing intracellular S. aureus (Fig. 6c), suggesting that some of the recent observations made in HeLa cells and CHO cells may also apply to HaCaT cells [50, 51].

Next, we analysed the conversion of EGFP-LC3-I to EGFP-LC3-II by Western blot [52]. Samples receiving mutant toxin remained negative. Samples from α-toxin-treated cells gave a strong band corresponding to the lipidated form of EGFP-LC3. Although this effect was reduced by 3-MA (Fig. 7a), inhibition of endosomal acidification with the V-ATPase-inhibitor bafilomycin enhanced it (Fig. 7b).

The above results suggested that α-toxin causes accumulation of autophagosomes in HaCaT cells, at least in part by enhancing autophagic flux, and by processes that are sensitive to the inhibition of PI3K. This prompted us to test the effect of 3-MA on the replenishment of intracellular ATP following sublethal α-toxin attack. Cells were preincubated with 3-MA and subsequently treated with α-toxin. Cellular ATP was measured 2 and 6 h after treatment. As shown in Fig. 7 c, the inhibitor strongly reduced the replenishment of cellular ATP observed in control cells.

Discussion

The principal result of this study is that membrane perforation triggers signals dedicated to the maintenance of energy and nutrient homoeostasis in cells. This provides a straightforward explanation for autophagy induction by PFT and helps to explain recovery after sublethal attack. Pro-autophagic signaling was readily observed under conditions of transient membrane perforation, with de-phosphorylation/re-phosphorylation kinetics of S6K closely following the course of metabolic crisis and recovery. This strongly suggests that accumulation of autophagosomes in perforated cells is not simply due to a block at some maturation step, or of the fusion with lysosomes. Consistently, bafilomycin enhanced toxin-induced lipidation of EGFP-LC3. Accumulation of autophagosomes was documented by electron microscopy and by fluorescence microscopy using EGFP-LC3 and antibodies against p62. Whereas autophagy-induction and colocalization with p62 have also been observed with listeriolysin [53, 54], a variant of aerolysin failed to recruit LC3 to membranes [55]. Clearly, further work is required to fully understand the requirements for autophagy induction by various PFT and the potential role of p62 and ubiquitination in this process.

We have shown previously that membrane perforation by various PFT induces loss of ATP in many cell types. Therefore, activation of AMPK and subsequent dephosphorylation of S6K in response to PFT did not come as a surprise. However, we show that PFT also activate GCN2. Although the established trigger of GCN2 in both mammalian and yeast cells is amino acid starvation [56], disturbances of ion homeostasis have been reported to activate the yeast homolog GCN2p [57, 58]. That amino acid transport into cells depends on ion gradients across the plasma membrane [47] provides a potential link between these events, and consequently between membrane perforation and activation of GCN2. Most likely, transport of other nutrients including glucose is similarly affected by PFT.

A second eIF2α-kinase, PKR, was also activated by α-toxin; however, the mechanism of activation remains to be elucidated. Because GADD34 overexpression reduced the accumulation of autophagosomes, while salubrinal enhanced it, eIF2α-phosphorylation appears to be involved in autophagy induction upon membrane perforation. The present data are the first to show that PFT trigger the ISR. Thus, PFT join LPS [33] and N-(3-oxo-acyl)homoserine lactones [59] in the list of bacterial products that can trigger this archaic stress response.

That membrane perforation is the common cause of pro-autophagic signal induction by PFT is suggested by the fact that structurally diverse toxins (SLO, HlyA and α-toxin) yielded similar results. By using a single amino acid exchange mutant of α-toxin which binds to cells, but is inefficient in pore formation, we show conclusively that membrane perforation is important for pro-autophagic signaling, accumulation of EGFP-LC3-positive puncta and lipidation of EGFP-LC3 in response to this PFT. Like in the case of p38, the efflux of potassium might be a common trigger for GCN2- and AMPK phosphorylation by PFT.

Previously, Schnaith et al. have observed that S. aureus induces autophagy in an agr-dependent way [51]. Extending on this observation, Mestre et al. have recently reported that S. aureus α-toxin is required for autophagy in mammalian cell lines infected with S. aureus [50], and here we have confirmed this observation for HaCaT cells. It was also observed that phagosome escape of S. aureus is agr dependent [60], and that S. aureus-containing or toxin-induced autophagosomes are not acidified [50, 51]. The latter observation is reminiscent of our finding that S. aureus α-toxin pore complexes are not degraded after endocytosis but released via exosome-like structures which we termed toxosomes [16]. However, in that study, we had also observed bafilomycin-sensitive degradation of α-toxin-monomers, indicating that under conditions of sublethal perforation, α-toxin does not fully paralyse the proteolytic machinery in human epithelial cells. Consistently, we found here that bafilomycin enhances EGFP-LC3-lipidation in α-toxin-treated HaCaT cells. Because α-toxin heptamers are rather resistant to proteolytic degradation under a broad range of conditions in vitro (our unpublished data), degradation of internalized heptamers is probably precluded, independent of a block of acidification.

It has been speculated that autophagy serves to degrade VCC [37]. Our previous work did not support the idea that this model applies to S. aureus α-toxin pore complexes [16], and the recent data of Mestre et al. confirm our conclusion [50]. Our model envisaging the release of toxosomes is also not at odds with the observation that induction of autophagy by nutrient deprivation inhibits exosome secretion [61], because pro-autophagic signaling fades with ensuing toxosome release (this study and [16]). Actually, an autophagy-dependent mode of exocytosis has recently been described in yeast [62, 63]. Therefore, we speculate that toxosome release might represent a manifestation of a similar pathway in mammalian cells.

3-MA inhibited the formation of EGFP-LC3-positive puncta and lipid-modified EGFP-LC3, suggesting that perforation of HaCaT cells with α-toxin triggers the canonical pathway of autophagy. Replenishment of ATP in α-toxin-treated cells was also inhibited by 3-MA. Among possible explanations for the apparent discrepancy between our results and those of Mestre et al. [50] are first that different cell types were investigated in the two studies, and second that we used far lower concentrations of α-toxin. Αlpha-toxin at low concentrations is known to form pores that are selective for monovalent ions, whereas high concentrations may permit influx of calcium [6466].

To sum, we have shown here that sublethal plasma membrane attack by PFT activates pro-autophagic signals, apparently due to transient nutrient and energy shortage. Evidence is presented that this depends on transmembrane pore formation. Because LC3- and p62-recruitment was not confined to vesicles containing α-toxin, the autophagic response observed here may be caused by global cellular starvation and energy loss. The relative contribution of these factors—and possibly of additional ones, like hypoxia [10]—to the accumulation of autophagosomes in perforated cells, or cells infected by PFT-producing bacteria remains to be established. Although autophagy may be life-saving for target cells of PFT (Fig. 8), it might come at a price, because it may promote replication of intracellular S. aureus and possibly persistence in the host, [51, 67, 68].

Fig. 8
Hypothetical model of induction and role of autophagy after membrane perforation by PFT: membrane perforation cuts nutrient supplies by paralysing ion gradient–dependent transport systems and causes energy loss. This leads to the activation of ...

Acknowledgments

We thank Prof. Yoshimori (Research Institute for Microbial Diseases, Osaka) for the generous gift of pEGFP-LC3. The skilful work of Regina Eberle is highly appreciated. Support from the Deutsche Forschungsgemeinschaft (SFB 490, project D3) is gratefully acknowledged. We thank S. Bhakdi for continuous support and critical comments on the manuscript.

Open Access This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.

Footnotes

N. Kloft and C. Neukirch contributed equally to this work.

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