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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
J Neurosci. Author manuscript; available in PMC 2010 October 14.
Published in final edited form as:
PMCID: PMC2954603
NIHMSID: NIHMS205858

NMDA Receptor Hypofunction Produces Opposite Effects on Prefrontal Cortex Interneurons and Pyramidal Neurons

Abstract

NMDA receptors mediate excitatory postsynaptic potentials throughout the brain but, paradoxically, NMDA receptor antagonists produce cortical excitation in humans and behaving rodents. To elucidate a mechanism for these diverging effects, we examined the effect of use-dependent inhibition of NMDA receptors on the spontaneous activity of putative GABA interneurons and pyramidal neurons in the prefrontal cortex of awake rats. We find that inhibition of NMDA receptors predominately decreases the activity of putative GABA interneurons but, at a delayed rate, increases the firing rate of the majority of pyramidal neurons. Thus, NMDA receptors preferentially drive the activity of cortical inhibitory interneurons suggesting that NMDA receptor inhibition causes cortical excitation by disinhibition of pyramidal neurons. These findings support the hypothesis that NMDA receptor hypofunction, which has been implicated in the pathophysiology of schizophrenia, diminishes the inhibitory control of PFC output neurons. Reducing this effect may be critical for treatment of schizophrenia.

Keywords: schizophrenia, glutamate, GABA, ensemble unit recording, antipsychotic drugs, metabotropic glutamate receptors

Introduction

Executive functions of the prefrontal cortex (PFC), including working memory and set-shifting, are critically dependent on NMDA receptors (Krystal et al., 1994; Verma and Moghaddam, 1996; Lisman et al., 1998; Stefani and Moghaddam, 2005). Hypo-function of NMDA receptors is implicated in the cognitive symptoms associated with many brain disorders, especially schizophrenia (Javitt and Zukin, 1991; Olney and Farber, 1995; Coyle, 1996). Oral or intravenous administration of NMDA receptor antagonists such as phencyclidine or ketamine produces transient schizophrenia-like symptoms in healthy individuals (Krystal et al., 1994, 2003; Adler et al., 1999; Newcomer et al., 1999) and impairs PFC-dependent cognitive functions in a manner that is indistinguishable from patients with schizophrenia (Adler et al., 1999). These agents also reinstate the pre-existing symptoms in stabilized patients with schizophrenia (Luby et al., 1959; Lahti et al., 1995b; Malhotra et al., 1997), suggesting that they tap into the same mechanisms that are already compromised in this disorder. These findings have generated great interest in identifying mechanisms by which NMDA receptor hypofunction influences the neuronal activity of the PFC. Animal and clinical work, however, have produced paradoxical results in that, as expected, NMDA antagonists inhibit EPSPs in PFC pyramidal neurons in slices, cultures, and anesthetized preparations (Hirsch and Crepel, 1991; Arvanov and Wang, 1997; Chen et al., 2003), but produce profound cortical activation in functional imaging studies in human volunteers (Lahti et al., 1995a; Breier et al., 1997; Vollenweider et al., 1997; Suzuki et al., 2002) or electrophysiological recording in behaving rodents (Jackson et al., 2004). The latter findings imply that tonic activation of NMDA receptors in an “awake” cortex has principally an inhibitory influence on pyramidal neurons, inconsistent with the conventional view that NMDA-mediated EPSPs contribute to spontaneous activity of these neurons.

In behaving animals, the activity of cortical pyramidal neurons is regulated by fast-spiking (FS) GABA interneurons (Wilson et al., 1994; Markram et al., 2004). These interneurons have faster EPSPs than pyramidal neurons and are more effectively recruited by excitatory inputs (Kawaguchi, 2001; Povysheva et al., 2006), suggesting that NMDA receptors may play a greater role in regulating the spontaneous firing of FS interneurons compared with pyramidal neurons. Thus, a plausible mechanism that might account for NMDA receptor antagonist–induced cortical excitation is that a reduction in NMDA receptor function preferentially reduces the firing rate of FS interneurons, resulting in disinhibition of pyramidal neurons. This will then lead to a net cortical excitation, which is typically observed after NMDA antagonist treatment in human or behaving animal studies (Breier et al., 1997; Vollenweider et al., 1997; Jackson et al., 2004). To test the validity of this mechanism, we compared the effect of a use-dependent NMDA antagonist on the spontaneous firing of putative inhibitory interneurons and pyramidal neurons in freely moving rats.

Materials and Methods

Details of surgical implantation, recording techniques and single-unit isolation have been described previously (Homayoun et al., 2004a; Homayoun and Moghaddam, 2006). Briefly, extracellular single-unit activity was recorded from medial PFC [(anteroposterior, +2.4–3.6; mediolateral, +0.5–0.8; dorsoventral, −3.5 mm, according to Paxinos and Watson (1998)] of freely moving rats. Extracellular neuronal activity was recorded from chronically implanted electrodes using Recorder software (Plexon, Dallas, TX). Spikes were sorted using Off-Line Sorter (Plexon) and single units were isolated on the basis of discrete waveform clustering, a minimum refractory period of 1.1 ms, stability of their waveform, interspike interval histogram, and autocorrelogram over time (average 2.2 U per electrode). Based on previous work (McCormick et al., 1985; Connors and Gutnick, 1990; Wilson et al., 1994; Tierney et al., 2004), we used the criteria of baseline firing rate >10 Hz combined with narrow average spike width to classify FS putative interneurons. FS units also were required to have a high-frequency component in their autocorrelation histograms and interspike interval histograms. Although this double criteria is strongly suggestive of interneurons and unlikely to present pyramidal neurons [regular spiking (RS)], it is possible that a small minority of interneurons could be misclassified as regular firing units. However, such potential misclassification of slow firing interneurons would have minimal impact on the latter group, which is expected to mainly contain the more easily detectable pyramidal neurons.

Data was pooled from 13 rats during 16 recording sessions, in which at least one putative interneuron was recorded per session. (It should be emphasized that putative interneurons were found in relatively few recording sessions and only in a subset of animals. For example, in the same period of time that the present data were collected, no FS units were found in 43 recording sessions.) Animals were injected with the use-dependent NMDA receptor antagonist dizocilpine maleate (MK801). The dose of MK801 (0.1 mg/kg, i.p.) was an optimal dose for producing deficits in working memory and set-shifting (Homayoun et al., 2004b; Stefani and Moghaddam, 2005) and increasing spontaneous activity of the majority of RS neurons in the PFC (Jackson et al., 2004). Data were analyzed during a 1 h window that included a 10 min baseline period and a 50 min postinjection period. A subset of rats (n =10; 149 RS and 18 FS units) had received a vehicle (saline or distilled water) injection 20 min before MK801. Another subset (n = 6; 80 RS and 14 FS units) had no pretreatment. The results obtained in the two subsets were similar in terms of both frequency of responses and their temporal dynamics (p > 0.05), and were thus pooled. A total of 6 U (2.2%) that did not meet one of the two criteria were excluded. Firing-rate histograms with 1 min bins (smoothed with a Gaussian filter with bin width of 3) were used to analyze the data. A significant response was defined as 10 consecutive bins crossing the 95% confidence intervals of the 10 min baseline, and responses were classified as excitatory, inhibitory or no change. The proportion of response types between groups was compared using χ2 test (p < 0.05). Population peridrug firing-rate time histograms were compared across RS and FS groups using two-way ANOVA with time as a repeated measure followed by Bonferroni post hoc test (p < 0.05). The onset of a significant response was assessed based on the first 1 min bin of a 10-bin change compared with baseline. To ensure that the effect of MK801 was not limited to a single dose, recordings were performed in a subset of rats (n = 4) with a higher dose of MK801 (0.3 mg/kg) (for results, see supplemental Fig. 1, available at www.jneurosci.org as supplemental material).

Results

Single units classified as FS neurons (n = 32 from 13 rats) had higher firing rates (Fig. 1A, abscissa, 16.67 vs 3.84 Hz) and narrower spike waveforms (Fig. 1A, ordinate, peak to valley width, 531.8 vs 272.6 μs) than RS neurons recorded from the same rats (n = 229). Examples of average (Fig. 1B) and composite (Fig. 1C) waveforms of RS and FS units are depicted. Note that spike waveforms remained stable after MK801 administration (Fig. 1D).

Figure 1
Distinction between putative interneurons (FS units) and pyramidal neurons (RS units). A, The population of putative interneurons had a higher firing rate (abscissa) and narrower spikeform (ordinate, average ± SEM of peak to valley spike width). ...

Representative examples of individual responses of FS and RS neurons (Fig. 2A,B) demonstrate divergent responses to systemic administration of MK801. FS neurons primarily displayed an inhibitory response whereas the majority of RS neurons had a profound excitatory response to MK801. As shown in Figure 2C, ~69% of FS neurons decreased their firing rate whereas 86% of RS, recorded during the same sessions, increased their firing rate after MK801. A χ2 test indicated a significant difference in distribution of responses to MK801 between populations of pyramidal neurons and interneurons (Fig. 2C) (χ2 = 100.42; p < 0.001). The population activity in response to MK801 showed a sustained average inhibitory response by FS neurons and excitatory response by RS neurons (Fig. 2D). Two-way ANOVA with time as repeated measure revealed a significant difference between average firing rate time histograms of two types of units (type, F(1,259) = 12.96, p <0.001; time, F(59,15281) = 2.71, p <0.001; type by time interaction, F(59,15281) = 5.94, p < 0.001). Furthermore, the predominant inhibitory effect on FS units was not an artifact of the dose of MK801 and was repeated using a higher dose of MK801 (0.3 mg/kg) in a distinct set of animals (supplemental Fig. 1, available at www.jneurosci.org as supplemental material).

Figure 2
Inhibitory effects of MK801 on putative interneurons. A, B, Examples of inhibitory and excitatory effects of MK801 on an individual interneuron (A) and a pyramidal neuron (B). Each panel depicts the firing rate histogram (bin, 1 min; smoothed with a Gaussian ...

Visual comparison of individual neuronal responses (Fig. 3A,B) and the population histograms (Fig. 2D) suggested that FS neurons started to inhibit their firing and reached their inhibitory plateau in advance of the onset and plateau of the excitatory response of the simultaneously recorded RS units. This observation was verified by plotting the delay to onset/plateau of the inhibitory response of interneurons recorded within each session against the average excitatory onset/plateau of concurrently recorded pyramidal cells (Fig. 3C, onset, linear regression analysis, r2 = 0.95, p < 0.001, D, plateau, r2 = 0.94, p < 0.001, insets, interval between corresponding inhibitory and excitatory onset and plateau).

Figure 3
MK801 inhibition of interneurons precedes its excitation of pyramidal cells. A, Superimposed firing-rate histogram of simultaneously recorded pairs of interneurons (bar graph) and pyramidal neurons (solid line). Arrow indicates the time of MK801 injection. ...

Discussion

These findings indicate that in an awake cortex, NMDA receptors preferentially regulate the firing rate of cortical GABA interneurons. The impact of NMDA receptor inhibition on pyramidal neuron activity was profoundly excitatory, suggesting that this effect is indirect and caused by disinhibition of GABA neurons that control the firing of pyramidal neurons. This conclusion is consistent with the observation that inhibition of putative interneurons by the NMDA receptor antagonist started earlier and reached its plateau before the excitation of pyramidal neurons. It should be emphasized, however, that the relatively long delay between the start/plateau of inhibitions and excitations in our sampled neuronal population suggests that blocking the NMDA receptors is influencing complex network dynamics that affect the firing rate of FS and RS neurons for sustained periods of time. This is consistent with a role for NMDA receptors in mediating excitatory reverberation and various network models that incorporate NMDA receptors in maintaining integrator circuits or temporal accumulation of spike activity that correlate with behavioral events (Koulakov et al., 2002; Wong and Wange, 2006).

Synaptic inhibition is necessary for spatial tuning of pyramidal neurons and synchronized firing of cortical networks and, thus, is thought to play a critical role in the “executive” functions associated with these regions (Goldman-Rakic, 1999; Kawaguchi, 2001; Markram et al., 2004). For example, blocking GABA receptors in the PFC and the resulting disinhibition of pyramidal neurons disrupts the tuning of these neurons during sensorimotor and mnemonic phases of a working memory task (Rao et al., 2000; Constantinidis and Goldman-Rakic, 2002). The present findings suggest that the activity of cortical GABA interneurons is highly sensitive to tonic regulation by NMDA receptors. The basal activity of pyramidal neurons, in contrast, was not directly regulated by NMDA receptors, but was susceptible to disinhibition. This concomitant interneuron inhibition–pyramidal disinhibition process supports the hypothesis that reduced NMDA function produces cortical excitation and impair PFC-dependent cognitive functions by disrupting inhibitory control of pyramidal neurons.

The PFC contains several different types of GABAergic interneurons, which are classified based on morphology and expression of calcium-binding proteins (Markram et al., 2004). Interneurons that express calbindin- or calretinin-binding proteins primarily target the distal dendrites of pyramidal neurons whereas interneurons that express parvalbumin (PV) synapse primarily on the axon initial segment and cell body proximal dendrites. The PV-containing interneurons are, therefore, in a better position to regulate the firing rate of pyramidal neurons. These neurons receive extensive excitatory input, which accounts for their high basal firing rates, and are sensitive to neurotoxic effects of NMDA receptor antagonists during development (Kawaguchi and Kubota, 1993; Jones and Buhl, 1999; Cochran et al., 2003; Goldberg et al., 2003). They have wide-spread axonal arborization with a single cell estimated to synapse onto >200 pyramidal neurons, thus inhibiting the firing rate of large groups of cortical afferents (Cobb et al., 1995). Because of methodological limitations associated with awake animal recording, we could not identify the class of FS neurons in our study; however, based on the fast baseline firing rate of theses neurons (Kawaguchi and Kubota, 1993), it may be hypothesized that the majority of them were PV-containing interneurons. This would explain the profound influence that inhibition of putative FS neurons had on disinhibiting the majority of pyramidal neurons recorded from the same electrode array.

Functional deficits in GABA interneurons have been reported in schizophrenia (Akbarian et al., 1995; Lewis et al., 2005), suggesting that NMDA receptor antagonists mimic the symptoms of schizophrenia by producing transient functional deficits in GABA interneurons. The NMDA hypofunction paradigm for modeling schizophrenia possesses both face validity, as it closely replicates a wide spectrum of schizophrenia-like symptoms (Krystal et al., 1994; Adler et al., 1999), and predictive validity, as it distinguishes between atypical and typical antipsychotics based on their ability to suppress abnormal PFC activity (Homayoun and Moghaddam, 2007), and has been the basis for discovery of a novel antipsychotic drug (Moghaddam and Adams, 1998; Patil et al., 2007). The construct validity of this model, however, has been limited to a wide variety of schizophrenia susceptibility genes encoding for proteins that could regulate the function of NMDA receptors (Moghaddam, 2003; Harrison and Weinberger, 2005). The present observation adds an important aspect to the construct validity of this paradigm by demonstrating that it is associated with reduced activity of putative GABA interneurons in the PFC. Thus, the hypothesized NMDA receptor hypofunction results in functional deficits of cortical GABA interneurons, which is a consistent pathological finding in schizophrenia (Coyle, 2004; Lewis et al., 2005). More importantly, our findings provide a clinically relevant mechanism where the resulting hyperactivity of pyramidal neurons (Jackson et al., 2004) and excessive glutamate spillover (Moghaddam et al., 1997) can be the basis for discovery of novel antipsychotic drugs (Moghaddam and Adams, 1998; Patil et al., 2007).

Supplementary Material

Figure 1

Acknowledgments

This work was supported by the National Institute of Mental Health.

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