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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
Alcohol Clin Exp Res. Author manuscript; available in PMC 2010 October 7.
Published in final edited form as:
PMCID: PMC2951264

Ethanol Teratogenesis in Five Inbred Strains of Mice



Previous studies have demonstrated individual differences in susceptibility to the detrimental effects of prenatal ethanol exposure. Many factors, including genetic differences, have been shown to play a role in susceptibility and resistance, but few studies have investigated the range of genetic variation in rodent models.


We examined ethanol teratogenesis in five inbred strains of mice: C57BL/6J (B6), Inbred Short-Sleep, C3H/Ibg, A/Ibg and 129S6/SvEvTac (129). Pregnant dams were intubated with either 5.8 g/kg ethanol (E) or an isocaloric amount of maltose-dextrin (MD) on day 9 of pregnancy. Dams were sacrificed on day 18 and fetuses were weighed, sexed and examined for gross morphological malformations. Every other fetus within a litter was then either placed in Bouin’s fixative for subsequent soft-tissue analyses or eviscerated and placed in ethanol for subsequent skeletal analyses.


B6 mice exposed to ethanol in utero had fetal weight deficits and digit, kidney, brain ventricle and vertebral malformations. In contrast, 129 mice showed no teratogenesis. The remaining strains showed varying degrees of teratogenesis.


Differences among inbred strains demonstrates genetic variation in the teratogenic effects of ethanol. Identifying susceptible and resistant strains allows future studies to elucidate the genetic architecture underlying prenatal alcohol phenotypes.

Keywords: Prenatal Alcohol, Teratogenesis, Inbred Strains, Genetics


Women who consume alcohol during pregnancy place their offspring at risk for a number of teratogenic outcomes. The most severe cases are diagnosed as Fetal Alcohol Syndrome (FAS), a disorder defined by prenatal and/or postnatal growth retardation, a characteristic pattern of craniofacial abnormalities and central nervous system dysfunction (Jones and Smith, 1973; Jones et al., 1973; Sokol et al., 2003). Because not all offspring exposed to alcohol prenatally display the full spectrum of FAS symptoms (particularly the facial dysmorphology), the term Fetal Alcohol Spectrum Disorders (FASD; Koren et al., 2003; Sokol et al., 2003) has been coined to describe varying degrees of ethanol teratogenesis, including FAS. The estimated incidence of FASD in the United States is 1% of live births (May and Gossage 2001; Sampson et al. 1997). Neurodevelopmental and behavioral deficits associated with prenatal ethanol exposure include developmental delay, attention deficits, hyperactivity, learning and memory impairments and diminished impulse control (Coles, 2001; Kelly et al., 1987; Kvigne et al., 2004; Sampson et al., 1997; Sokol et al., 2003).

A number of other physiological and morphological malformations have been reported following in utero ethanol exposure. These include renal anomalies (Assadi 1990; DeBeukelaer et al. 1977; Qazi et al. 1979; Taylor et al. 1994), limb and digit malformations (Froster and Baird 1992; Jones et al. 1973; Jones and Smith 1975; Pauli and Feldman 1986; Smith et al. 1981; van Rensburg 1981), dilated brain ventricles and other brain ventricle abnormalities (Clarren et al. 1978; Jones and Smith 1973, 1975; Konovalov et al. 1997) and skeletal malformations (axial and vertebral; Smith et al. 1981; Tredwell et al. 1982; Tsukahara and Kajii 1988). Interestingly, some of the skeletal defects reported following prenatal alcohol exposure are similar to anomalies seen in Klippel-Feil Syndrome (defects of segmentation or fusion of cervical and thoracic vertebra and rib anomalies; Lowry 1977; Neidengard et al. 1978; Schilgen and Loeser 1994).

Not all women who consume ethanol during pregnancy give birth to children with observable deficits, which demonstrates individual differences in susceptibility to ethanol teratogenesis. Many risk factors have been shown to contribute to the development of FASD, including the amount, timing and pattern of ethanol exposure, maternal age and parity, maternal ethnicity and socioeconomic status, cultural factors, maternal smoking and other drug abuse, maternal stress and psychological state, maternal diet/nutrition, maternal education, employment and marital status (Abel, 1995; Abel and Hannigan, 1995; Leonardson and Loudenberg, 2003).

Studies have also shown that genetic differences partially explain why some offspring of women who consume alcohol during pregnancy are severely affected while others are not (Chasnoff, 1985; Christoffel and Salafsky, 1975; Palmer et al., 1974; Riikonen, 1994). These studies indicate that monozygotic twins are more similarly affected than dizygotic twins. A more comprehensive study examined ethanol exposure in utero in both monozygotic and dizygotic twins. The rate of concordance for diagnosis was 5/5 for monozygotic twins and 7/11 for dizygotic twins and the authors concluded that genes had a modulating influence on the teratogenic effects of alcohol (Streissguth and Dehaene, 1993). More recently, several studies have shown that different alleles of the alcohol dehydrogenase gene (ADH), an enzyme involved in ethanol metabolism, can influence the severity of teratogenesis in different ethnic populations (Das et al., 2004; McCarver et al., 1997; Stoler et al., 2002; Viljoen et al., 2001).

Mice are an excellent model organism for investigating genetic effects on many phenotypes, and are well suited for studying the effects of prenatal ethanol exposure (Driscoll et al., 1990). Several studies used inbred strains and selectively bred mice to demonstrate genetic variation in many teratogenic outcomes, including embryolethality, development of various brain structures, fetal weight gain, and digit, skeletal, ocular, renal and heart anomalies (Boehm et al., 1997; Cassells et al., 1987; Chernoff, 1977, 1980; Downing and Gilliam, 1999; Giknis et al., 1980; Gilliam and Irtenkauf, 1990; Gilliam et al., 1997; Persaud and Sam, 1992; Wainwright and Gagnon, 1985; Webster et al., 1980). Nevertheless, only a few inbred strains have been examined and the range of genetic variation is unknown for any prenatal ethanol phenotypes.

In the present study, we examined the effects of prenatal ethanol exposure on morphological malformations in five inbred strains of mice: Inbred Short-Sleep (ISS), C57BL/6J (B6), C3H/Ibg (C3H), A/Ibg (A) and 129S6/SvEvTac (129). The Inbred Long-Sleep (ILS) and ISS strains were derived by inbreeding Long-Sleep (LS) and Short-Sleep (SS) mice, selectively bred for differential sensitivity to a hypnotic dose of alcohol (McClearn and Kakihana, 1981). Previous research has shown that LS mice are more susceptible to some of the teratogenic properties of ethanol than SS mice (Gilliam and Kotch 1990, 1996; Gilliam et al., 1989a; Gilliam et al, 1989b). Because LS mice are also more susceptible to the hypnotic effects of ethanol, it suggests that one or more genes may mediate both the soporific and teratogenic properties of ethanol in these two lines. ILS and ISS have not been examined for ethanol teratogenesis. Due to poor reproduction, ILS mice were unavailable during the course of this study.

B6 is one of the most widely used inbred strains of mice in all of biomedical research. They are susceptible to fetal weight deficits and kidney, limb and skeletal malformations following prenatal ethanol exposure (Boehm et al., 1997; Downing and Gilliam, 1999; Gilliam and Irtenkauf, 1990; Gilliam et al., 1997; Webster et al., 1980). In this study they serve as a positive control for known teratogenic effects. One study examined the A strain (A/J from the Jackson Laboratory) and showed them to be more susceptible to skeletal malformations than even B6 following prenatal ethanol exposure (Boehm et al. 1997). We wanted to examine the A/Ibg substrain for similar effects. Three studies have looked at teratogenesis in C3H following prenatal ethanol exposure and found them to be susceptible to fetal weight deficits and brain and skeletal malformations (Chernoff 1977, 1980) and resistant to prenatal mortality (PNM) and soft-tissue malformations (Lochry et al. 1981). We wanted to confirm these effects in the C3H/Ibg substrain. To the best of our knowledge, no 129 strains of mice have been examined for morphological malformations following prenatal ethanol exposure. Results from this study will further characterize the range of genetic variation in ethanol teratogenesis in mus musculus.



Male and female B6, ISS, C3H, A and 129 mice were generated and housed in the specific pathogen-free (SPF) facility at the Institute for Behavioral Genetics, Boulder, CO. ISS mice were created at IBG and thus have the /IBG substrain designation. A mice were originally purchased from the Jackson Laboratory, but have been maintained for many years at IBG separate from the Jackson colony and genetic drift may have occurred, so they too have the /IBG designation. Likewise, the C3H mice were originally brought to IBG by Dr. Gerald McClearn from the University of California at Berkley in the 1960s and also have the /IBG designation. The 129 mice were recently obtained by IBG from Taconic and therefore still have the /Tac designation, while B6 mice were recently re-derived from Jackson Laboratory stock and have the /J designation. Males were individually housed while females were housed three to five per cage; mice were maintained on a 12-hr light/dark cycle (lights on at 0700) and were given food and water ad libitum. The temperature was kept at a constant 22° (± 2 °) C. All procedures were approved by the University of Colorado Institutional Animal Care and Use Committee, in accordance with National Institute of Health guidelines.

Mating and Dosing

Two females were placed with a male for two hours each morning (7:00 am – 9:00 am) and examined for a seminal plug as evidence of mating. The morning of plug detection was designated gestational day 0 (GD 0). Because A females did not plug well when mated for two hours in the morning, we began to mate them overnight; 15 of the 21 A litters were generated by mating overnight, while 6 A litters were generated by mating for 2 hours in the morning. Plugged females were weighed and single-housed. At noon on GD 9, females were weighed to ascertain a 2 gram minimum weight gain as evidence of pregnancy. Females were then intragastrically intubated with either 5.8 g/kg ethanol (E) or an isocaloric amount of maltose-dextrin (MD). We chose the 5.8 g/kg dose on GD 9 because this is a sensitive period during organogenesis and we can reliably reproduce a pattern of malformations in the susceptible B6 strain. On GD 18, females were sacrificed at 2:00 pm; uterine horns were exposed and a count made of live, dead and resorbed fetuses. Live fetuses were weighed, sexed and examined for gross morphological malformations. Every other fetus within a litter was placed in Bouin’s fixative a minimum of four weeks for subsequent soft-tissue analyses using Wilson’s (1965) freehand slicing method. The remaining fetuses were placed in ethanol a minimum of 2 weeks. They were then macerated in a 1% KOH solution for 72 hours. Fetuses were then placed in a 1% KOH solution containing alizarin red for 6–9 hours. Stained fetuses/skeletons were then placed in 25% glycerin for 24 hours and then stored in 75% glycerin for subsequent skeletal analyses.


Upon c-section, all fetuses were examined for gross morphological malformations, which consisted primarily of forepaw adactyly (missing digits) and syndactyly (fused digits). Soft-tissue malformations consisted of dilated brain ventricles and kidneys (hydronephrosis). Skeletal malformations included missing and fused ribs and asymmetrical, fused or missing vertebral arches and centra. All teratological exams were done blind, without knowledge of strain or treatment.

Data Analysis

Maternal data were examined using analysis of variance (ANOVA) with strain (ISS, B6, A, C3H and 129) and treatment (E or MD) as between group factors. Offspring data were analyzed with strain, treatment and sex as between group factors All analyses were performed using Statistical Package for the Social Sciences (SPSS, v. 15.0). Maternal variables included weight gain and prenatal mortality. Offspring variables included weight at c-section and morphological malformations. Litters with only one pup were not used for analyses. For all offspring variables, litter means (percent litter malformed for malformations) were the unit of analysis; male and female means were analyzed separately. Because we found no effect of sex on any measure of teratogenesis, we collapsed across sex for analyses and all figures depict combined male and female litter means. Post hoc analyses consisted of Bonferoni corrected t-tests within strain. An alpha level of .05 was used to assess significant results. Sample sizes were as follows: ISS-E 15 litters, ISS-MD 13; B6-E 13, B6-MD 12; 129-E 10, 129-MD 13; C3H-E 13, C3H-MD 13; A–E 11, A-MD 10.


Maternal Data

Maternal Weight Gain

Percent maternal weight gain was calculated as: [(weight day 18 – weight day 9)/weight day 9]. There was a significant main effect of strain (F(4, 112) = 5.56, p < .001), but no main effect of treatment (F(1, 112) = 3.59, p < .062) and no strain by treatment interaction. As can be seen in Table 1, ISS dams put on less weight than all the other strains except C3H (p’s < .01). Even though ANOVA showed no main effect of treatment and there was no significant strain by treatment interaction, a t-test showed that B6 MD-treated dams put on more weight than E-treated B6 dams (p < .05), which is clearly evident in Table 1. Maternal weight gain was also calculated with fetal weight subtracted out [(weight day 18 – weight day 9 – fetal weight)/weight day 9] to examine whether differences in fetal weight could account for differences in maternal weight gain. With fetal weight subtracted out (adjusted percent maternal weight gain or APMWG), ANOVA showed a significant main effect of strain (F(4,112 = 6.124, p < .01) but no significant effect of treatment and no significant strain by treatment interaction. There were no differences in APMWG in B6 E- and MD-treated dams (Table 1) which shows that the difference found in PMWG in B6 dams was due to differences in fetal weight.

Table 1
Mean (± SEM) percent maternal weight gain, prenatal mortality and offspring weight at c-section. Sample size is shown in parentheses under treatment for each strain

Prenatal Mortality

Prenatal mortality was calculated by dividing the number of resorptions plus dead fetuses by the number of implantation sites. There were significant main effects of strain (F(4, 112) = 9.24, p < .001) and treatment (F(1, 118) = 4.13, p < .05), but no significant interaction. C3H dams had greater prenatal mortality than B6, 129 (p’s < .001) and ISS (p < .03) dams; E-treated dams had greater prenatal mortality than MD-treated dams (p < .03; Table 1).

Offspring Data

Fetal Weight

Fetal weight was defined as pups’ weight at c-sectioning. There were significant main effects of strain (F(4, 209) = 67.46, p < .001) and treatment (F(1, 209) = 16.44, p < .001), but no significant interactions among variables. Post hoc analyses showed that A pups weighed significantly less than all strains except ISS (p’s < .001); E-treated pups weighed less than MD-treated pups (p < .001).

Gross Morphological Malformations

Upon c-section, pups were examined for gross morphological malformations, which consisted primarily of forepaw adactyly and syndactyly. These two measures were combined to give a measure of digit malformations. There were significant main effects of strain (F(4, 209 = 4.76, p < .001) and treatment (F(1, 209) = 10.77, p < .001) and a significant strain by treatment interaction (F(4, 209) = 5.87, p < .001). Post hoc analyses showed that compared to MD-controls, E-treated B6 (p < .01) and C3H (p < .03) fetuses had increased digit malformations (Table 2).

Table 2
Mean (± SEM) litter percent for digit, kidney, brain ventricle, rib and vertebral malformations. Sample sizes are indicated in parentheses under strain and treatment.

Soft Tissue Malformations

After examination for gross morphological malformations, half the fetuses per litter were stored in Bouin’s fixative for subsequent soft tissue analyses. Soft tissue anomalies included dilated brain ventricles and hydronephrosis (Figures 1A and 1B). For dilated ventricles we found significant main effects of strain (F(4, 177) = 9.49, p < .001) and treatment (F(1, 177) = 14.23, p < .001) and a significant strain by treatment interaction (F(4, 177) = 4.50, p < .003). Compared to MD-controls, E-treated ISS (p < .02) and A (p < .024) fetuses had significantly more malformations. For kidney malformations (Figure 2) we also found significant main effects of strain (F(4, 177) = 6.44, p < .001) and treatment (F(1, 177) = 8.01, p < .01) and a significant strain by treatment interaction (F(4, 177) = 2.25, p < .05). Compared to MD-treated controls, E-treated B6 (p < .01) fetuses had significantly more kidney malformations (Table 2).

Figure 1Figure 1
Figure 1A. Normal lateral and third brain ventricles taken from a gestational day 18 mouse fetus exposed to maltose-dextrin prenatally.
Figure 2
Kidneys taken from a gestational day 18 mouse fetus exposed to alcohol prenatally. While the kidney on the right is normal, the kidney on the left exhibits hydronephrosis.

Skeletal Malformations

After gross examination, the remaining half of the fetuses in each litter were eviscerated and stored in ethanol for subsequent skeletal examinations. Rib malformations included missing or fused ribs, while vertebral malformations included missing, fused or asymmetrical arches and centra (Figures 3A–3C). For rib malformations we only found a significant main effect of treatment (F(1, 166) = 4.38, p < .04); E-exposed fetuses had a significantly higher percentage of rib malformations than MD-exposed fetuses (Table 2). For vertebral malformations, ANOVA showed no significant main effects or interactions.

Figure 3Figure 3Figure 3
Figure 3A. Skeleton from a gestational day 18 mouse fetus exposed to maltose-dextrin prenatally.


C57BL/6J is the most widely used inbred mouse strain in alcohol research and has been well characterized for several prenatal phenotypes. B6 are susceptible to fetal weight deficits, digit, kidney, brain ventricle and vertebral malformations following ethanol exposure during the period of organogenesis (Boehm et al. 1997; Downing and Gilliam 1999; Gilliam and Irtenkauf 1990; Gilliam et al. 1989b, 1997; Johnson et al. 2007; Persaud and Sam 1992; Randall et al. 1977, 1988; Webster et al. 1980, 1983; Zimmerman et al. 1990). Our results support these findings. Thus far, B6 seems to represent the extreme of ethanol teratogenesis, consistently showing effects on most measures.

Long-Sleep (LS) and Short-Sleep (SS) mice were selectively bred for sensitivity and resistance to the sedative properties of alcohol (McClearn and Kakihana 1981). Studies have shown that following acute or chronic ethanol exposure, LS mice are susceptible to fetal weight deficits and rib and skeletal malformations, but are resistant to PNM and digit, kidney and brain ventricle malformations (Gilliam and Irtenkauf 1990; Gilliam et al. 1989a, 1989b). In contrast, SS mice are resistant to fetal weight deficits, PNM, and digit, skeletal and soft-tissue malformations following prenatal ethanol exposure (Gilliam et al. 1989a, 1989b, 1997). This suggests that one or more genes mediating susceptibility and resistance to the soporific effects of alcohol may also mediate, at least partly, the teratogenic effects of ethanol. Because pregnant LS and SS dams show similar blood ethanol concentrations at various timepoints following ethanol exposure, the differences in teratogenesis are likely not due to differences in ethanol metabolism (Gilliam et al. 1989b). While ILS mice were unavailable in the present study, we found that ISS mice are resistant to fetal weight deficits, PNM and digit, kidney, and skeletal malformations following in utero ethanol exposure. In contrast with SS, ISS mice are quite susceptible to dilated brain ventricles.

We found that following an acute dose of ethanol on GD9, C3H/Ibg mice are resistant to fetal weight deficits, PNM and kidney, brain ventricle and vertebral malformations; they are susceptible to digit and rib malformations. Using a paradigm similar to ours, Lochry et al. (1982) exposed C3H/HeJ mice to a single dose of ethanol (0, 2.5 or 5.0 g/kg) during gestation (GD 7, 8, 9, 10, 11, 12, 14, 16 or 18) and reported no effects on resorptions, dead fetuses or soft-tissue malformations. Chernoff (1977, 1980) reported that C3H/lgM1 mice are susceptible to fetal weight deficits, dilated brain ventricles and skeletal malformations following prenatal ethanol exposure. However, comparisons to the Chernoff studies are difficult due to differences in substrains used, dosing regimens, teratological assessment techniques and data analyses.

Boehm et al. (1997) reported that pregnant A/J dams put on less weight than controls following ethanol exposure and had increased PNM. Fetuses from the A strain were susceptible to weight deficits and rib and vertebral malformations, while they were resistant to digit and kidney malformations; they were not examined for dilated brain ventricles. We found pregnant A/Ibg females to be resistant to weight gain deficits and PNM. Offspring from the A strain were resistant to fetal weight deficits and digit, kidney and skeletal malformations following prenatal ethanol exposure; they were quite susceptible to dilated brain ventricles. The biggest difference in A mice between our study and the Boehm et al. (1997) study was for skeletal malformations. While Boehm et al. reported litter percentages for ethanol-induced rib, arch and centra malformations from 40–58%, our study found percentages 0–18%. Boehm et al. used A mice obtained from the Jackson Laboratory while our (IBG) mice, originally obtained from JAX, have been maintained separately for over 30 generations. It is possible that genetic drift has occurred in our colony and that our mice are slightly different (genetically) from the JAX stock. One or more of these mutations may explain the differences in skeletal malformations.

To the best of our knowledge, none of the many 129 substrains has been examined for ethanol teratogenesis. We found that 129 mice, when administered 5.8 g/kg on GD 9, are resistant to all measures of teratogenesis. This finding is important because 129 substrains of mice are often used to create targeted gene mutations (knockouts or KOs). Most mouse KO studies report the use of embryonic stem cells derived from 129 mice to create the KO construct; constructs are then injected into B6 blastocysts to create KOs. Rendering a gene nonfunctional is one way to test whether the gene has an effect on a phenotype of interest. Therefore, because most mouse KOs have at least some 129 genome in their background, it is important to characterize this strain for all phenotypes of interest, including prenatal ethanol traits.

Despite the uniformity in time of exposure, it is possible that the inbred strains used in the present study are at slightly different developmental stages. One of the only ways to definitively determine where mice are, developmentally, is to stage somites, something we did not do. However, between strains, between litters of the same strain and within litters of the same strain, there can be a great deal of variation in somitogenesis (Gossler and Tam, 2002; Tam 1981; Thiel et al. 1993). It has been shown that even in the same litter, embryos can be at different stages of development and (at any given time) can vary by 3–8 somites or ~ 6–17 hours (Gossler and Tam 2002). It is therefore impossible to ensure that all embryos are at the same developmental timepoint when we intubate on GD 9 and have all those fetuses for teratological examinations on GD 18. This may account for some of the effects, or lack thereof, in this study. Previous research from our laboratory has shown that following 5.8 g/kg ethanol on GD 9, GD 9 and GD 10, or GD 7 through GD 15, B6 mice are quite susceptible to ethanol teratogenesis while SS mice are relatively resistant (Gilliam et al. 1989a, 1989b, 1997). In addition, A mice are susceptible to skeletal malformations following ethanol exposure on GD 9 (Boehm et al. 1997). Thus, in the present study we are fairly certain that we are dosing ISS, B6 and A mice at a sensitive developmental timepoint. No such data exist for the 129 and C3H strains. Future studies need to examine a number of different inbred strains and timepoints to further characterize sensitive developmental timepoints for various organ systems.

Previous studies from our laboratory have shown that following a 5.8 g/kg dose of ethanol, pregnant SS, B6 and A dams show no differences in ethanol metabolism, averaging 350–450 mg% one hour after exposure (Boehm et al. 1997; Gilliam and Irtenkauf 1990; Gilliam et al. 1989b, 1997). Unpublished data from our laboratory has also shown that the inbred ISS and ILS do not differ in ethanol metabolism from the selectively bred SS and LS. Therefore, we are fairly confident that the differential teratogenesis seen in the ISS, B6 and A strains are not due to differences in ethanol metabolism. However, pregnant C3H and 129 dams have not been examined for BECs following a similar dosing regimen so it is possible that the teratogenesis we observed in C3H and the lack of effect seen in 129 are due to differential ethanol metabolism.

Results from this study further the knowledge of genetic variation in ethanol teratogenesis. Such knowledge will facilitate identification of susceptibility and resistance genes in mice, which can then be examined in human populations. We and others have outlined a strategy, using mus musculus, which allows one to go from identifying a genetic influence on a phenotype of interest to identifying the causal gene(s) and DNA sequence polymorphisms (Downing et al. 2005). The first step is demonstrating genetic variation for the phenotype of interest, usually by identifying inbred strain differences or through selective breeding. Then susceptible and resistant strains are crossed to create populations for quantitative trait locus (QTL mapping). After initial QTL mapping, various recombinant strategies are used to narrow QTL regions, typically to one megabase or smaller, in order to identify candidate genes mediating phenotypic differences. Finally, several strategies can be used to functionally evaluate candidate genes. These strategies have been used to identify and evaluate candidate genes for many alcohol-related phenotypes, including consumption and preference, locomotor activation, loss-of-righting reflex and several measures of tolerance (Bennett et al. 2006; Crabbe et al. 1999; Downing et al. 2005; Treadwell 2006). Somewhat surprisingly, for all prenatal ethanol phenotypes, researchers are still at step one: identifying genetic variation. Towards this end, we have recently reported preliminary results from QTL mapping for ethanol teratogenesis in recombinant inbred strains derived from a cross between the susceptible B6 strain and the resistant DBA/2J (D2) strain (BXD RIs; Downing et al. 2008)


This study was supported by grants AA016676 and AA01466. The authors would like to thank Ms. Alexi Kimball for her excellent technical assistance.


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