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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
J Neurosci. Author manuscript; available in PMC 2010 September 29.
Published in final edited form as:
PMCID: PMC2947440
NIHMSID: NIHMS179268

Acute in vivo genetic rescue demonstrates that phosphorylation of RIM1α serine 413 is not required for mossy fiber LTP

Abstract

While presynaptic, protein kinase A (PKA)-dependent, long-term plasticity has been described in numerous brain regions, the target(s) of PKA and the molecular mechanisms leading to sustained changes in neurotransmitter release remain elusive. Here we acutely reconstitute mossy fiber long-term potentiation (mfLTP) de novo in the mature brain of mutant mice that normally lack this form of plasticity. These results demonstrate that RIM1α, a presynaptic scaffold protein and a potential PKA target, can support mfLTP independent of a role in brain development. Using this approach, we study two mutations of RIM1α (S413A and V415P) and conclude that PKA-phosphorylation-dependent signaling by RIM1α serine 413 is not required for mfLTP, consistent with conclusions reached from the study of RIM1α S413A knockin mice. Together, these results provide insights into the mechanism of mossy fiber LTP and demonstrate a useful acute approach to genetically manipulate mossy fiber synapses in the mature brain.

Keywords: presynaptic LTP, mossy fiber, synaptic plasticity, RIMS1, hippocampus, Sindbis

Introduction

Synaptic plasticity, the modulation of synaptic efficacy, has long been considered to be a primary neural mechanism for learning and memory (Kandel 2001; Fusi et al., 2005). Synaptic plasticity may be expressed either presynaptically, through changes in neurotransmitter release, or postsynaptically, through alterations in neurotransmitter receptor activity or localization. Many forms of presynaptic long term plasticity require signaling through cyclic AMP and cAMP-dependent protein kinase A (PKA) (Huang et al., 1994; Weisskopf et al., 1994, 1995; Tzounopoulos et al., 1998; Villacres et al., 1998; Nguyen and Woo 2003, Chevaleyre et al., 2007). In addition to PKA signaling, a requirement for the presynaptic proteins, Rab3a and RIM1α, has also been demonstrated (Castillo et al., 1997; Castillo et al., 2002; Chevaleyre et al., 2007; Fourcaudot et al., 2008). Yet how the activities of these components are orchestrated to produce long lasting changes in synaptic efficacy remains poorly understood.

RIM1α is an active zone scaffold protein that binds to many presynaptic proteins involved in neurotransmitter release and synapse formation (Wang et al., 1997, 2000, 2002; Zhen and Jin 1999; Betz et al., 2001; Coppola et al., 2001; Wang et al., 2001; Ohtsuka et al., 2002; Schoch et al., 2002; Sun et al., 2003; Kiyonaka et al., 2007). RIM1α has been shown to be required for PKA-dependent, presynaptic LTP at hippocampal mossy fiber synapses, cerebellar parallel fiber synapses (Castillo et al., 2002) and cortical-lateral amygdala synapses (Fourcaudot et al., 2008), as well as PKA-dependent, presynaptic LTD at hippocampal inhibitory synapses (Chevaleyre et al., 2007). Because RIM1α contains PKA phosphorylation sites, it has been proposed to be the downstream effector of PKA signaling in presynaptic long-term plasticity (Lonart el al., 2003; Sudhof 2004). This hypothesis was strengthened by the observation that acutely expressing RIM1α with a mutated PKA phosphorylation site failed to restore presynaptic LTP to RIM1α−/− neurons and suppressed LTP in wild-type neurons that were cultured from embryonic cerebellum (Lonart et al., 2003). However, examination of a knockin mouse with this same mutation (S413A) failed to find any defects in presynaptic LTP, either at parallel fiber or mossy fiber synapses (Kaeser et al., 2008). These conflicting results raise concerns that the expression of presynaptic LTP in the knockin mouse may have resulted from developmental compensation and/or that the results from cultured neurons may not accurately reflect in vivo mechanisms.

Therefore, we sought to address the role of PKA-mediated phosphorylation of serine 413 in mfLTP by means of acute genetic manipulation in the mature brain. Here we describe a method to reconstitute mfLTP de novo by genetically manipulating hippocampal granule cells in the adult brain through in vivo viral injection. Our approach bypasses the issue of developmental compensation inherent to knockin strategies and avoids concerns about ex vivo neuronal differentiation, synapse development and connectivity that are raised by cultured neuron preparations. We demonstrate that mfLTP can be acutely restored in RIM1α−/− mice when RIM1α is expressed only briefly in the mature brain, indicating that RIM1α is not required during the major developmental period of synapse formation for its role in mfLTP. We further show that phosphorylation of RIM1α serine 413 is not required to acutely restore mfLTP in the mature, developed brain.

Materials and Methods

Plasmids and concentrated Sindbis virus preparation

Rat RIM1α cDNA was kindly provided by Dr. Thomas Sudhof and contains the Δ83-105 splice variant at splice site A (Wang et al., 1997; Lonart et al., 2003). RIM1α cDNA was subcloned into the pSINrep(nsP2S726) vector (provided by Dr. Pavel Osten (Kim et al., 2004)). An internal ribosomal entry sequence (IRES) and EGFP cDNA were subcloned from pSR5I2E (Marie et al., 2005) and inserted 3′ of the RIM1α cDNA. Missense mutations were introduced using the Quikchange site-directed mutagenesis kit (Stratagene). Recombinant Sindbis virus was generated according to the manufacturer’s instructions (Invitrogen, Carlsbad, CA). In vitro transcription was performed using Ambion mMESSAGE mMACHINE SP6 kit (Applied Biosystems/Ambion, Austin, TX) and products were electroporated into BHK21 cells. Recombinant viral particles were harvested 60–72 hr after electroporation, purified by centrifugation at 3,000 rpm (Marathon 22K centrifuge, Fisher Scientific, Pittsburgh, PA), and concentrated by ultra-centrifugation at 100K × g (Beckman L8M ultracentrifuge, Beckman Coulter, Inc., Fullerton, CA). The pellet was re-suspended in PBS. Viral titers were estimated to be 0.5 – 1 × 106 TU/μl for all viruses as detected by EGFP fluorescence following HEK cell infection. Control virus expressed only EGFP downstream of the IRES sequence. Test viruses expressed RIM1α cDNA upstream of the IRES sequence and EGFP. Viral identities were unknown to the investigator during in vivo injection, data acquisition and analysis.

Stereotaxic injection

All surgeries were done in accordance with guidelines for humane care and handling of rodents and with approval from our institutional IACUC. To reduce procedural variability, surgeries were performed on batches of 4 to 10 mice at a time. Four-week-old mice were anesthetized with avertin (2-2-2 tribromoethanol) intraperitoneally and placed in a stereotaxic mouse head holder (David Kopf Instruments, Tujunga, CA). Fifty nanoliters of concentrated nsP2S726 modified Sindbis virus were injected (flow rate of 23 nl/sec) into the dentate gyrus of the hippocampus bilaterally at 6 different depths. The initial injection coordinates relative to bregma were anterior 1.95 mm, mediolateral 1.2 mm, dorsoventral 2.00 mm. Additional 50 nl injections occurred each 0.05 mm during withdrawal of the injection cannula. Virus was delivered using Nanoject II (Drummond Scientific, Broomall, PA) and the cannula remained at each location for 1 minute following each injection.

RIM1α−/− mice were obtained as previously described (Schoch et al., 2002) and backcrossed to c57/Bl6 at least six times prior to use. To further reduce genetic background variability, experiments testing viral effect on mfLTP in RIM1α−/− mice were performed on littermates. Littermate RIM1α−/− mice were obtained from RIM1α−/− × RIM1α−/− or RIM1α−/− × RIM1α+/− breedings so that sufficient numbers (i.e.>1) of RIM1α−/− mice could be obtained per litter. All homozygote parents of experimental subjects were obtained from the breeding colony which was maintained exclusively by RIM1α−/+ × RIM1α−/+ matings. Experiments testing the effect of control EGFP virus infection on mfLTP in wild type mice were performed on wild type mice obtained from RIM1α−/+ × RIM1α−/+ breedings.

Electrophysiology

Four to seven days after viral injection, acute transverse hippocampal slices (300 μm thick) from virus-injected mice were prepared and used for experiments. Infected slices were selected for use in experimental recordings by the presence of EGFP fluorescence detectable in more than half of the area of the dentate gyrus viewed in the horizontal plane (Fig. 1B). Recordings were done in normal aCSF containing (in mM): 119 NaCl, 2.5 KCl, 1.3 MgSO4, 2.5 CaCl2, 1.0 NaH2PO4, 26.2 NaHCO3 and 10 D-glucose, saturated with 95% O2 and 5% CO2 and perfused at 2 ml/minute. A monopolar stimulation electrode with 2M NaCl was placed in a region of stratum granulosum with EGFP fluorescence. Mossy fiber responses were monitored at room temperature by extracellular field potentials (fEPSPs) from the stratum lucidum in the CA3 area at a frequency of 0.05 Hz unless otherwise indicated. Baseline stimulation was adjusted to yield fEPSP responses between 0.4 and 0.7 mV. The average stimulus intensity was 0.33 ± 0.03 mA for control EGFP viral slices and 0.29 ± 0.03 mA for test RIM viruses (p = 0.18). Mossy fiber contribution to the field potentials was confirmed at the end of each experiment by sensitivity to pharmacological blockade by 3 μM DCG-IV, a group II metabotropic glutamate receptor agonist that selectively suppresses mossy fiber synaptic transmission (Nicoll and Schmitz 2005; Gibson et al, 2005). Data were included only if the remaining response was less than 10% of the initial baseline response. Mossy fiber LTP was induced by a single tetanus at time 0 min. (25 Hz for 5s) in the presence of an NMDA-type antagonist (50 μM APV) during tetanic induction as previously described (Castillo et al., 2002). Mossy fiber LTP is reported as the average fEPSP amplitude during the period 50–60 min. post-tetanus relative to the baseline period (10 min. prior to tetanus). Data were acquired at 10 kHz and filtered at 1 kHz.

Figure 1
Postnatal viral-mediated expression of RIM1α rescues mossy fiber LTP in RIM1α−/− mice, without altering short-term plasticity. (A) Schematic representation of the experimental strategy. (B) Representative example of an ...

Data Analysis

All summary data are presented as mean ± S.E.M. as a percentage of the baseline. To avoid false-positives from multiple testing, repeated measure ANOVA and Kruskal-Wallis test were performed on all data from different groups (66 slices total, 7 groups, p < 10−3 for both tests). Subsequent ad hoc comparisons were performed using Student’s t-test and Wilcoxon rank order test. Both tests yielded similar conclusions. For brevity, only results from student’s t-test are presented. In all figures, an asterisk (*) indicates p-value <0.05, exact values are stated in text.

Results

Acute expression of RIM1α in RIM1α−/− mice restores mossy fiber LTP

To acutely rescue mfLTP in RIM1α −/− mice, we took advantage of the high infectivity and low toxicity of an nsP2S726 mutant Sindbis virus. The S726 mutation of the nsP2 protein impairs the ability of the virus to shut down host protein synthesis and has markedly reduced toxicity (Supp. Fig. 1; Dryga et al., 1997; Jeromin et al., 2003; Kim et al., 2004). In order to manipulate synapses in the most physiological setting, virus was delivered to the dentate gyrus of 4-week-old mice by in vivo stereotaxic injection. We observed that nsP2S726 Sindbis virus had a very high infectivity of granule cells (30–92%, Supp. Fig. 2 and Supp. Methods) such that we could routinely visualize EGFP fluorescence in greater than half of the area of the dentate gyrus (viewed in the horizontal plane of acute transverse hippocampal slices) (Fig. 1B). We reasoned that this high degree of infectivity should produce a detectable effect at the population level; therefore, we chose to monitor synaptic responses using field excitatory postsynaptic potentials (fEPSP). In this way, variability related to uncertain connectivity of any individual postsynaptic cell was minimized. To decrease variation due to degree of infection, only slices with greater than 50% of the dentate gyrus fluorescent were used for recording.

In RIM1α −/− slices infected with the control EGFP virus, mossy fiber fEPSP responses displayed characteristic properties of short-term plasticity and sensitivity to the group II metabotropic glutamate receptor agonist, DCG-IV, and were similar to those observed in non-injected control slices (Supp. Fig. 3A,B). Similarly, infection by the control EGFP virus did not alter synaptic responses in RIM1α −/− slices following the mfLTP induction protocol, nor did it impair the degree of potentiation in RIM1α +/+ slices (Supp. Fig. 3C,D). These findings indicate that the in vivo viral manipulation of hippocampal granule cells does not significantly alter mossy fiber responses.

We next examined whether acute expression of RIM1α in hippocampal granule cells in the mature brain was able to rescue mfLTP in RIM1α−/− mice. Following mfLTP induction, evoked mossy fiber responses from slices expressing RIM1α exhibited long-lasting potentiation of fEPSPs (Fig. 1C,D and Supp. Fig. 4, RIM1α 135 ± 6%, 11 slices/7 mice). In contrast, slices expressing the EGFP control virus failed to exhibit appreciable mfLTP (EGFP 114 ± 4%, 12 slices/5 mice; p = 0.009 RIM1α vs. EGFP in RIM1α −/− slices). The average percentage of mfLTP after RIM virus infection in RIM1α −/− slices is approximately 70% of that observed in wildtype RIM1α +/+ slices (Figure 1C,D; EGFP in RIM1α +/+ slices 151 ± 6%, 6 slices/3 mice; p = 0.096 RIM1α in RIM1α −/− slices vs. EGFP in RIM1α +/+ slices). This is not surprising given that the extracellular field potentials represent activity from a population average of infected and uninfected cells. However, it is notable that, in some experiments, we do obtain mfLTP values comparable to the highest noted in wildtype recordings suggesting that it is possible to rescue to wildtype levels (data not shown).

We next evaluated whether acute expression of RIM1α altered short-term plasticity of mossy fiber synapses. We found no significant changes in paired-pulse ratios, frequency facilitation or sensitivity to DCG-IV as a result of acute RIM1α expression (Fig. 1E, F). These findings in conjunction with a previous study which demonstrated that embryonic deletion of RIM1α did not change mossy fiber short-term plasticity (Castillo et al., 2002) suggest that RIM1α plays a significant role in long-term but not short-term plasticity at these synapses.

The phosphorylation site-deficient mutant of RIM1α rescues mossy fiber LTP in RIM1α−/− mice

Using this acute, in vivo rescue approach, we examined the role of RIM1α serine 413 phosphorylation in mfLTP. A serine to alanine substitution at amino acid 413 was made to prevent phosphorylation at this site, as had been done in previous studies (Lonart et al., 2003; Kaeser et al., 2008). Following S413A mutant RIM1α viral infection, robust mfLTP could still be observed (Fig. 2) (145 ± 7%, 12 slices/6 mice; p = 0.001 compared to EGFP; p = 0.28 compared to RIM1α). These results indicate that acute expression of either S413A mutant or wild type RIM1α in hippocampal granule cells can restore mfLTP in young adult RIM1α−/− mice.

Figure 2
Postnatal viral-mediated expression of S413A mutant RIM1α rescues mfLTP in RIM1α−/− mice. Traces are from periods immediately before (solid trace) and 60 min after (dashed trace) tetanus. Sample sizes were: EGFP 12 slices/5 ...

The enhanced-14-3-3-binding mutant of RIM1α does not occlude LTP at mossy fiber synapses in RIM1α−/− mice

It has been proposed that phosphoserine-dependent binding of 14-3-3 proteins to RIM1α at the S413 residue is also required for the expression of presynaptic LTP. In support of this idea, a V415P mutation of RIM1α, which markedly enhances phospho-serine 413 binding to 14-3-3 proteins in vitro, has been shown to enhance basal release and occlude LTP in cerebellar cultures from RIM1α−/− mice (Simsek-duran et al., 2004). These observations provide a second opportunity to test the relevance of S413 phosphorylation in our in vivo rescue platform. In contrast to the cerebellar culture results, acute in vivo expression of V415P mutant RIM1α was able to rescue mfLTP (Fig. 3, 149 ± 10%, 12 slices/7 mice; p = 0.004 compared to EGFP; p = 0.25 compared to RIM1α). Thus, two distinct mutations that interfere with phosphoserine-dependent signaling of RIM1α at the S413 site demonstrate that this is not a requirement for the expression of presynaptic LTP at mossy fiber synapses.

Figure 3
Postnatal viral-mediated expression of the enhanced 14-3-3 binding mutant V415P of RIM1α rescues mfLTP in RIM1α−/− mice. Traces are from periods immediately before (solid trace) and 60 min after (dashed trace) tetanus. ...

Discussion

In contrast to the ease of manipulation and advances in understanding postsynaptic physiology enabled by acute genetic manipulation of postsynaptic proteins (Pettit et al., 1994; Lissin et al., 1998; Takahashi et al., 2003), similar investigations of presynaptic physiology have been far more limited. Unlike the study of postsynaptic proteins, where one can directly patch on a neuron that expresses a fluorescent marker indicating infection and modification of all postsynaptic sites, acute manipulation of presynaptic proteins is limited by technical hurdles related to the often sparse and variable connectivity of genetically modified inputs to postsynaptic cells. Previous methods for overcoming this limitation have included using paired recordings of pre- and post-synaptic cells (Lonart et al., 2003; Wimmer et al., 2004), autaptic cultured neurons in which a single cell synapses only with itself (Rhee et al., 2002), and herpes viral infection of rat granule cells to inhibit mfLTP (Dumas et al., 1999). However, these methodologies present significant limitations. First, not all brain circuits have sufficient connectivity in brain slices to make paired recordings feasible, as is the case between the dentate gyrus and CA3 (Nicoll and Schmitz 2005). Second, culture models raise the possibility of divergence from in vivo mechanisms. Third, few viruses can achieve both the high infectivity and low toxicity necessary to overcome issues raised by low or variable connectivity of genetically modified inputs. As a result, most studies investigating the molecular mechanisms of presynaptic LTP have relied upon the generation of transgenic mouse models (Villacres et al., 1998; Castillo et al., 1997, 2002; Kaeser et al., 2008) - an approach that is costly, time-intensive, and makes it difficult to distinguish direct effects from those resulting from developmental compensation. Here we provide a novel approach for investigating the molecular mechanisms of presynaptic LTP that circumvents many of these limitations.

In this study, we have, for the first time, acutely reconstituted mfLTP in the mature brain of a mutant mouse that normally lacks this form of LTP. We employed the RIM1α −/− genetic background in these studies because to date, the mechanism by which RIM1α is required for mfLTP is unknown. RIM1α interacts with many presynaptic proteins that each could potentially be involved in mediating presynaptic long-term plasticity. For example, the interaction with α-liprin, a protein involved in active zone organization (Zhen and Jin, 1999), suggests that RIM1α may play a role in synapse formation. Because acute expression of RIM1α in the mature brain can restore mfLTP, our results suggest that a role for RIM1α in synapse development is unlikely to underlie its requirement for mfLTP.

We also used this approach to test whether there was evidence to support the idea that S413 of RIM1α serves as a phosphorylation-dependent switch required for the expression of presynaptic LTP. This idea gained support from a series of studies that acutely rescued LTP in cerebellar cultures prepared from embryonic RIM1α−/− mice (Lonart et al., 2003; Simsek-duran et al., 2004). However, in contrast to this idea, both cerebellar parallel fiber and hippocampal mossy fiber LTP were intact in the genetic knockin mouse, which contained an S413A mutation in the RIM1 gene (Kaeser et al., 2008). Here we show that two mutations interfering with the phosphorylation-dependent signaling of RIM1α serine 413 (S413A and V415P) are both capable of restoring presynaptic LTP acutely using intact, post-development brain circuitry. These findings are in agreement with the S413A knockin mouse study and bypass concerns about developmental compensation.

Several differences remain between the two acute genetic rescue approaches that might account for the divergent results. For example, plasticity mechanisms might be different between cerebellar granule cell-Purkinje cell synapses and hippocampal mossy fiber synapses; although in brain slice studies, parallel fiber and mossy fiber LTP appear to have shared mechanisms and the S413A knockin mutation left LTP intact at both of these sites (Nicoll and Schmitz 2005; Kaeser et al., 2008). Alternatively, plasticity mechanisms might differ between neurons cultured from embryonic tissue and those from intact adult brain slices. Lastly, mechanisms responsible for the divergent results might engage in the one-day window between the time of the cultured neuron observations (20 – 54 hrs) and our mfLTP recording period (4 – 7 days).

In summary, we show for the first time that mfLTP can be reconstituted de novo in the mature brain of mice normally lacking this form of plasticity. Our results demonstrate that the role of RIM1α in mfLTP is not due to a requirement for RIM1α during the major period of brain development. Using this acute rescue approach, we provide two lines of evidence that phosphorylation of RIM1α S413 is not required for mfLTP, indicating that the mechanism for the requirement of PKA in presynaptic LTP is still unknown. Our approach provides a useful tool in the otherwise limited armamentarium to pursue this question as well as investigate more generally the mechanisms of presynaptic long-term plasticity.

Supplementary Material

Supp1

Acknowledgments

The authors would like to thank Yuanji Pan and Samantha Tracy for outstanding technical assistance, Thomas Sudhof for providing RIM1α−/− mice, Jing Lu for guidance with in vivo techniques, members of the Calakos lab for stimulating discussions, and Drs. G. Augustine, J. McNamara, R. Mooney and A. West for critical reading of the manuscript. Funding was provided by NINDS P01(NS053862) and K02 (NS054840) grants to N.C.

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