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J Bacteriol. 1980 November; 144(2): 852–855.
PMCID: PMC294742

Transposed LEU2 gene of Saccharomyces cerevisiae is regulated normally.

Abstract

The repression of beta-isopropylmalate dehydrogenase, the LEU2 gene product, by leucine and leucine plus threonine was unaffected by the transposition of LEU2 from its original locus on chromosome III to a new locus within the ribosomal deoxyribonucleic acid gene cluster on chromosome XII. Since the expression of the LEU2 gene is probably controlled at a pretranslational level, we conclude that the recombinant plasmid used for transformation carries regulatory information in addition to LEU2 structural information.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Brown HD, Satyanarayana T, Umbarger HE. Biosynthesis of branched-chain amino acids in yeast: effect of carbon source on leucine biosynthetic enzymes. J Bacteriol. 1975 Mar;121(3):959–969. [PMC free article] [PubMed]
  • Carbon J, Ratzkin B, Clarke L, Richardson D. The expression of cloned eukaryotic DNA in prokaryotes. Brookhaven Symp Biol. 1977 May 12;(29):277–296. [PubMed]
  • Dietz AA, Lubrano T. Separation and quantitation of lactic dehydrogenase isoenzymes by disc electrophoresis. Anal Biochem. 1967 Aug;20(2):246–257. [PubMed]
  • Hinnen A, Hicks JB, Fink GR. Transformation of yeast. Proc Natl Acad Sci U S A. 1978 Apr;75(4):1929–1933. [PubMed]
  • Hsu YP, Kohlhaw GB. Leucine biosynthesis in Saccharomyces cerevisiae. Purification and characterization of beta-isopropylmalate dehydrogenase. J Biol Chem. 1980 Aug 10;255(15):7255–7260. [PubMed]
  • Petes TD. Meiotic mapping of yeast ribosomal deoxyribonucleic acid on chromosome XII. J Bacteriol. 1979 Apr;138(1):185–192. [PMC free article] [PubMed]
  • Petes TD. Yeast ribosomal DNA genes are located on chromosome XII. Proc Natl Acad Sci U S A. 1979 Jan;76(1):410–414. [PubMed]
  • Petes TD. Unequal meiotic recombination within tandem arrays of yeast ribosomal DNA genes. Cell. 1980 Mar;19(3):765–774. [PubMed]
  • Ratzkin B, Carbon J. Functional expression of cloned yeast DNA in Escherichia coli. Proc Natl Acad Sci U S A. 1977 Feb;74(2):487–491. [PubMed]
  • Satyanarayana T, Umbarger HE, Lindegren G. Biosynthesis of branched-chain amino acids in yeast: regulation of leucine biosynthesis in prototrophic and leucine auxotrophic strains. J Bacteriol. 1968 Dec;96(6):2018–2024. [PMC free article] [PubMed]
  • Szostak JW, Wu R. Insertion of a genetic marker into the ribosomal DNA of yeast. Plasmid. 1979 Oct;2(4):536–554. [PubMed]
  • VOGEL HJ, BONNER DM. Acetylornithinase of Escherichia coli: partial purification and some properties. J Biol Chem. 1956 Jan;218(1):97–106. [PubMed]

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