Search tips
Search criteria 


Logo of jcinvestThe Journal of Clinical Investigation
Published online 2010 September 1. doi: 10.1172/JCI43165

Figure 1

An external file that holds a picture, illustration, etc.
Object name is JCI43165.f1.jpg
TRPC6 in the neurons was downregulated in ischemia.

(A) Immunoblots of the extracts from the contralateral (C) or ipsilateral (I) cortex (sham; left [L] or right [R] hemisphere) using the indicated antibodies. Tubulin served as a loading control. Right panel: quantification of the normalized TRPC6 protein levels (n = 5–8 rats per time point). *P < 0.05; **P < 0.01 versus sham. (B) Immunoblots for TRPCs and GluR1 after 24 hours reperfusion (R24). Right panel: quantification of the protein levels (n = 5 rats). **P < 0.01 versus contralateral. (C) TRPC6 mRNA levels determined by qRT-PCR (n = 3–5 rats per time point). (D) Representative images of the indicated cortex after 24 hours reperfusion (R24) double-stained with the indicated antibodies. Scale bar: 50 μm. Quantification of the optical density for both TRPC6 and NeuN immunoreactivity is shown on the right panel (n = 5 rats). **P < 0.01 versus the density in contralateral cortex. Data are presented as mean ± SEM. Unless stated, TRPC6 antibody from Millipore was used for Figures Figures1,1, ,2,2, and and66 and antibody from Alomone Labs was used for Figures Figures3,3, ,4,4, ,5,5, and and7. 7.

Images in this article

  • Figure 1
  • Figure 2
  • Figure 3
  • Figure 4
  • Figure 5
  • Figure 6
  • Figure 7
Click on the image to see a larger version.