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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
Biol Psychiatry. Author manuscript; available in PMC 2011 October 1.
Published in final edited form as:
PMCID: PMC2942977
NIHMSID: NIHMS208914

ELEVATED GAMMA-AMINOBUTYRIC ACID LEVELS IN CHRONIC SCHIZOPHRENIA

Abstract

Background

Despite widely-replicated abnormalities of gamma-aminobutyric acid (GABA) neurons in schizophrenia postmortem, few studies have measured tissue GABA levels in vivo. We used proton magnetic resonance spectroscopy to measure tissue GABA levels in participants with schizophrenia and healthy controls in the anterior cingulate cortex (ACC) and parieto-occipital cortex (POC).

Methods

21 schizophrenia participants effectively treated on a stable medication regimen (mean age 39.0, 14 male) and 19 healthy controls (mean age 36.3, 12 male) underwent a proton magnetic resonance spectroscopy scan using GABA-selective editing at 4 Tesla after providing informed consent. Data were collected from two 16.7cc voxels and analyzed using LCModel.

Results

We found elevations in GABA/Cr in the schizophrenia group compared with controls (F(1,65)=4.149, p=0.046) in both brain areas (15.5% elevation in ACC, 11.9% in POC). We also found a positive correlation between GABA/Cr and Glu/Cr which was not accounted for by %GM or brain region.

Conclusions

We found elevated GABA/Cr in participants with chronically treated schizophrenia. Postmortem studies report evidence for dysfunctional GABAergic neurotransmission in schizophrenia. Elevated GABA levels, whether primary to illness or compensatory to another process, may be associated with dysfunctional GABAergic neurotransmission in chronic schizophrenia.

Keywords: MRS, MEGAPRESS, GABA, glutamate, anterior cingulate cortex, psychosis

INTRODUCTION

Clinical manifestations of schizophrenia are proposed to arise from abnormal information processing in the cerebral cortex(1). Because coordination of activity in pyramidal neurons is modulated by the inhibitory neurotransmitter gamma-aminobutyric acid (GABA), abnormal GABAergic neurotransmission may lead to compromised cortical processing and symptom expression(13). Indeed, a large literature implicates GABAergic interneuron abnormalities in schizophrenia(4,5). Postmortem studies report reduced message and expression of GAD67 (the synthetic enzyme for GABA), and of GAT1, the transporter that clears synaptic GABA, as well as an apparent compensatory upregulation in postsynaptic GABAA receptors in the dorsolateral prefrontal cortex (DLPFC) and anterior cingulate cortex (ACC)(4,6). The literature on tissue GABA measurements in schizophrenia is more discrepant, however, with reports of normal(7) and reduced brain GABA levels(8). Cerebrospinal fluid (CSF) studies report normal (7), reduced (9), or elevated (10,11) GABA in schizophrenia.

Despite great interest in GABAergic dysfunction in schizophrenia few published studies have examined brain GABA levels in vivo. Goto et al recently used proton magnetic resonance spectroscopy (MRS) at 3 Tesla in participants with early schizophrenia and reported GABA/Creatine (Cr) reductions in the striatum but not two cortical regions(12). In this study, we used proton MRS to measure brain GABA/Cr levels in a cohort of chronically ill, stably treated participants with schizophrenia at 4 Tesla. We used a GABA-selective editing MRS sequence termed MEGAPRESS(13,14). We collected data from the anterior cingulate cortex (ACC), where GABAergic abnormalities are reported in schizophrenia(5,15) and the parietal occipital cortex (POC) as a control region. Based on reports of abnormal GABAergic interneurons in the ACC postmortem, we hypothesized reduced GABA/Cr in the ACC but normal GABA/Cr in the POC in schizophrenia.

METHODS

Participants

Following approval by McLean Hospital IRB, 19 control and 21 schizophrenia participants provided informed consent (NC and SZ groups, respectively). All participants with schizophrenia were chronically ill and receiving antipsychotic treatment (except one). Participants with significant neurological or medical problems, current substance abuse or history of substance dependence (except tobacco smoking) were excluded. All participants had negative urine toxicology. Female participants were in the luteal phase of the menstrual cycle. Participants with schizophrenia were assessed using the Structured Clinical Interview for DSM-IV (SCID), Young Mania Rating Scale (YMRS), Montgomery-Asberg Depression Rating Scale (MADRS), and Positive and Negative Syndrome Scale (PANSS) on scan day. Three participants in the SZ group met criteria for schizoaffective disorder; these participants were chronically psychotic, not currently in a mood episode. Demographic and clinical variables are in Table S1 in the Supplement.

MRI/MRS Procedures

Proton MRS acquisitions were conducted on a 4 Tesla MR scanner (Varian/UnityInova, Varian Inc., Palo Alto, CA), using a 16-rung, single-tuned, volumetric-birdcage coil (XLR Imaging Inc, London, Ontario, Canada (www.xlrimaging.com). See the Supplement for details of anatomical imaging, MRS voxel positioning, and the MEGAPRESS sequence. Briefly, 23x22x33mm (16.7cc) voxels were placed on the ACC or POC, and MEGAPRESS(13) was implemented for GABA editing. Total time in magnet was under 75 minutes. Using the same procedures, we also carried out a test-retest study to determine reliability of our GABA/Cr measures as described in the Supplement.

MRS processing was blinded to diagnosis. For each voxel, the 'off' scans were subtracted from the 'on' scans to yield a difference-edited MEGAPRESS spectrum. In addition, the 'off' scans were averaged to yield a standard PRESS (TE = 68 ms) proton MRS spectrum. We used LCModel (version 6.0–1)(16) with a MEGAPRESS-specific phantom-acquired basis set (see Supplement). Out of 80 possible voxels, 71 usable datasets were obtained. There were no group differences in Cr levels and Cr was used as an internal reference to reduce subject-specific sources of variance in the MRS signal.

Statistics

Demographic and clinical variables were compared across groups using ANOVA, t-tests, and chi-square tests (Table S1 in the Supplement). Given our a priori hypothesis, we considered the GABA/Cr ratio as our primary outcome measure. We also calculated GABA/NAA and GABA/Cr* using a T2-corrected Cr resonance magnitude at 68ms based on our reported T2 relaxation times(17). Diagnosis effects were investigated using MANOVAs. We calculated parallel statistics for Glu/Cr and NAA/Cr levels and also examined medication effects on metabolite levels using one-way ANOVAs with medication status (e.g. taking or not taking benzodiazepines) as the between-subjects variable. All p-values are 2-tailed.

RESULTS

GABA/Cr levels showed a significant main effect of diagnosis (F(1,65)=4.149, p=0.046) and main effect of brain region (F(1,65)=40.617, p<0.001) but no brain region x diagnosis interaction (F(1,65)=0.147, p=0.702). GABA/Cr was elevated by 15.5% (ACC) and 11.9% (POC) in SZ (Table 1). Two sample t-tests within each brain region did not yield significant GABA/Cr differences (t=1.523, p=0.137 in ACC; t=1.214, p=0.234 in POC). Three participants with schizoaffective disorder had GABA/Cr values (0.113±0.019 in the ACC and 0.080±0.309 in the POC) similar to the SZ group as a whole. Both the GABA/NAA analysis (16.3%/12.9% elevation in ACC/POC; F(1,65)=4.334, p=0.042) and the T2 relaxation time corrected GABA/Cr analysis (13.7%/8.2% elevation in ACC/POC; (F(1,65)=3.033, p=0.087) showed a similar pattern (Table 1); the latter analysis was significant at a trend level.

Table 1
Metabolite levels (Mean±SD)

There were no significant main effects of brain region or diagnosis and no brain region x diagnosis interaction on Glu/Cr (not shown) (Table 1). There was a significant main effect of brain region on NAA/Cr (F(1,65)=33.279, p<0.001) but no diagnosis main effect and no brain region x diagnosis interaction (not shown) (Table 1). See the Supplement for correlation analyses.

CPZ equivalents were not correlated with GABA/Cr (Pearson’s R=-0.074, p=0.669), Glu/Cr, or NAA/Cr (not shown). In a series of one-way ANOVAs, GABA/Cr levels were no different between participants who were/were not taking second generation antipsychotics (F(19,1)=0.079, p=0.781), first generation antipsychotics (F(19,1)=0.921, p=0.348), benzodiazepines (F(19,1)=0.913, p=0.350), anticonvulsants (F(19,1)=3.122, p=0.091) or lithium (F(19,1)=0.458, p=0.506). When we repeated our primary analysis excluding schizophrenia participants taking anticonvulsants, the diagnosis effect on GABA/Cr was not significant (F(1,46)=0.229, p=0.634) (Figure S3 in the Supplement).

DISCUSSION

We found elevated GABA/Cr levels in a group of chronically ill, stable participants with schizophrenia using proton MRS at 4 Tesla. The abnormality existed in both brain areas studied, suggesting that it extends outside the ACC. Interestingly, this finding is the reverse of our a priori hypothesis. We did not observe NAA/Cr or Glu/Cr abnormalities in this study although NAA/Cr reductions are widely reported in schizophrenia(18). Potential explanations for the discrepancy include clinical status or small sample size.

Strengths of this study include high magnetic field, metabolite-selective data acquisition and analysis, and focus on two distinct brain regions. We evaluated our GABA quantification in a test-retest study where our reliability measures were comparable to previous reports(19). The spectral signal-to-noise ratios and CRLBs were similar across the two study groups, suggesting comparable overall spectral quality and fitting. By collecting data from two brain regions, we showed that GABAergic abnormalities in schizophrenia are not selective to the ACC. This is consistent with postmortem reports of GABAergic abnormalities in multiple cortical regions in schizophrenia(20). The ACC and POC are functionally linked through the default mode network, however, and other regions may show different patterns.

The major limitation of this study is that all participants except one were taking medications on a stable regimen. Many psychotropic medications have prominent effects on GABAergic neurotransmission and we cannot rule out medication effects on GABA/Cr measures in our study. We made attempts to examine medication effects in our data: there was no correlation of CPZ equivalents and GABA/Cr, and no evidence that lithium, anticonvulsants, or benzodiazepines impact GABA/Cr measures. Anticonvulsants can affect GABA levels and there was a trend towards anticonvulsant effect on GABA/Cr levels in our data. When we repeated our analysis excluding participants on anticonvulsants, the diagnosis effect on GABA/Cr was no longer significant, suggesting that we cannot rule out a role for anticonvulsant medication effects in our GABA/Cr findings. These subgroup analyses are underpowered due to small N, and well-designed medication-effect studies are ultimately needed to examine this issue.

Although Cr’s utility Cr as internal reference has been questioned because Cr levels may change in disease states, internal referencing to Cr did not skew our results because there were no group differences in Cr. One alternative to internal referencing with Cr would be using the water resonance but we did not acquire useable water unsuppressed spectra.

Our findings provide evidence for abnormalities in cortical GABAergic neurotransmission in schizophrenia. The literature on postmortem and CSF GABA levels in schizophrenia is equivocal with reductions, no change, or elevations in GABA CSF in chronically treated participants(10,11). One MRS study showed reduced GABA/Cr in the basal ganglia(12) but little information was provided regarding voxel location/GM-WM-CSF composition/spectral quality, thus precluding direct comparison with our study. Many sources of noise including medication/substance effects contribute to discrepancies in the literature; large well-controlled studies are needed to reconcile the multiple findings. We propose that an abnormality in GABAergic neurotransmission in schizophrenia may be associated with elevated brain GABA levels. Thus, there may be an uncoupling of neurotransmitter tissue levels and efficacy of neurotransmission in some circumstances.

Figure 1
(A) Midsagittal (left) and axial (right) views demonstrate ACC and POC voxel placement on a canonical individual T1-weighted image – not individual subject data. (B) Sample MEGAPRESS spectra from the ACC in a control subject (left) and subject ...

Supplementary Material

01

Acknowledgments

This study was funded by 5K23MH079982 (DO) from the National Institute of Mental Health, and sponsored in part by the Counter-Drug Technology Assessment Center, an office within the Office of National Drug Control Policy, via Contract Number DABT63-99-C awarded by the Army Contracting Agency. The content of the information does not necessarily reflect the position or the policy of the Government and no official endorsement should be inferred. This project also was sponsored in part by NIH grant S10 RR13938.

Footnotes

Financial Disclosure: Dr. Renshaw is a consultant to Novartis, GlaxoSmithKline, Kyowa Hakko, and has received research support from Eli Lilly, GlaxoSmithKline, and Roche. None of the other authors reported any biomedical financial interests or potential conflicts of interest.

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