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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
Cancer Biol Ther. Author manuscript; available in PMC 2010 September 20.
Published in final edited form as:
Cancer Biol Ther. 2004 November; 3(11): 1051–1057.
Published online 2004 November 18.
PMCID: PMC2941887
NIHMSID: NIHMS225235

Death Receptor Signals to Mitochondria

Abstract

Apoptosis is the best-characterized form of programmed cell death (PCD) and is of fundamental importance in tissue homeostasis. In mammalian systems, there are two major pathways that are involved in the initiation of apoptosis: the “extrinsic” death receptor pathway and the “intrinsic” mitochondrial pathway. Although these pathways act independently to initiate the death machinery in some cellular systems, in many cell types, including numerous tumor cells, there is delicate coordination and cross talk between the extrinsic and intrinsic pathways, which leads to the activation of the executioner caspase cascade. Additionally, there appears to be a fine balance between the caspase-mediated arm of death receptor signaling that engages mitochondria and the caspase-independent arm that promotes vacuole proliferation in many cells. Here, we review our current knowledge about the layers of complexity that are posed by the interactions between death receptor-induced pathways and how they influence mitochondria to regulate cellular life and death decisions.

Keywords: death receptors, mitochondria, Bid, membranes, phospholipases, cardiolipin

INTRODUCTION

Apoptosis, or programmed cell death, is an evolutionarily conserved mechanism for the selective removal of aging, damaged or otherwise unwanted cells.17 It is an essential component of many normal physiological processes such as embryogenesis, normal tissue development and the immune response.8 Thus, regulation of apoptosis is critical for tissue homeostasis and its deregulation can lead to a variety of pathological conditions. Inhibition of apoptosis or resistance to apoptosis contributes to carcinogenesis and chemoresistance.2,914 On the other hand, enhanced apoptosis is involved in diverse diseases such as myocardial ischemia, neurodegenerative diseases, stroke, septic shock and AIDS.11,12

Apoptosis is primarily mediated through the activation of specific proteases called caspases (cysteinyl, aspastate-specific proteases).2,3,1517 Caspases are effectors of cell suicide and cleave multiple substrates leading to biochemical and morphological changes that are characteristic to apoptotic cells.5,7 These alterations include cell membrane re-modeling and blebbing, exposure of phosphatidylserine at the external surface of the cell (PS), cell shrinkage with cytoskeletal rearrangements, nuclear condensation and DNA fragmentation.1,3,11,1820 These morphological changes culminate in the formation of apoptotic bodies that are normally eliminated by phagocytosis.21,22 In mammalian systems, the “extrinsic” death receptor pathway and the “intrinsic” mitochondrial pathway are the two major signaling systems that result in the activation of the executioner caspases, and the consequent induction of cell death.2,3,5,7,18 In the past few years, increasing evidence indicates that the death receptor and mitochondrial pathways are not isolated systems. Instead significant cross-talk and ‘biofeedback’ regulates the apoptotic machinery.7,9,23,24 In this review, we discuss some recent insights into the interconnections between these apoptotic pathways.

THE DEATH RECEPTOR PATHWAY OF APOPTOSIS

The extrinsic apoptotic pathway is activated upon the binding of cytokine ligands (i.e., FasL, TNF and TRAIL) to members of the TNFα receptor super-family, which are usually called the death receptors (i.e., Fas, also called CD95/Apo-1; TNF receptors; TRAIL receptors).2,3,7,1820 Death receptors contain an intracellular globular interaction domain known as a death domain (DD). Death receptors aggregate at the cell surface following ligand binding to their extracellular domains—possibly to form trimers. This results in the recruitment of adaptor molecules to the aggregated intracellular domains of the receptors. One of the major adaptors to be recruited is Fas Associated Death Domain, FADD, which possesses a DD that interacts either directly with the DD of death receptors, or indirectly through another adaptor molecule, TRADD (TNF Receptor Associated Death Domain). FADD also contains a second protein interaction domain, known as the Death Effector Domain (DED). The DED domain of FADD interacts with the DED of the weakly active zymogen, pro-caspase-8, to form an intracellular multi-protein complex known as the Death Inducing Signaling Complex (DISC).2630 Once formed, the DISC promotes the proximity-induced activation of caspase-8, which then proceeds to be further processed via an auto-proteolysis mechanism.31,32 Whether still bound to the DISC or released in other intracellular compartments, active caspase-8 activates executioner/effector caspases, such as caspase-3, leading to cell execution via degradation of the nucleus and other intracellular structures.18,29,33,34 This direct activation of caspase-dependent cell execution, which does not require mitochondria, is believed to occur in select cell types, including thymoctyes, that are classified as Type I cells.27,33,35 These cells are able to efficiently activate caspase-8, so that its major target is downstream cleavage and consequent activation of executioner caspases such as caspase-3. This simplified pathway of Type I cells plays an important role in the immune response that is involved in the deletion of transformed cells9,36 and resembles the linear pathway of developmental cell death established in genetic studies of C. elegans.37,38 Nonetheless, programmed cell death (PCD) in C. elegans is distinct in that Bcl-2/Ced-9 is unable to block caspase activation following death receptor stimulation in Type I cells.18,39 Consequently, the simplified extrinsic pathways of mammalian Type I cells is likely to result from a reductionist pattern of evolution.

THE MITOCHONDRIAL PATHWAY OF APOPTOSIS

Mitochondria are now thought to be the central intracellular organelles involved in mediating the majority of apoptotic pathways in mammalian cells.25,4044 In general, mitochondria are engaged via the intrinsic pathway of cell death, which can be initiated by a variety of stress stimuli including UV radiation, γ-irradiation, heat, DNA damage, the actions of some oncoproteins and tumor suppressor genes, viral virulence factors, and most chemotherapeutic agents.40 These diverse forms of stress are sensed or decoded by multiple cytosolic or intra-organellar molecules, which then transduce the signals to mitochondria, resulting in alterations of the outer mitochondrial membrane (OM).25,42,43,45,46 This initial ‘scarring’ of the OM leads to increased permeability to proteins that are normally trapped between the OM and the inner mitochondrial membrane (IM), thus enabling these proteins to escape the mitochondria and diffuse into the cytosol. The IM is a highly convoluted, protein-rich membrane with unusual lipid composition.25,4447 Oxidative phosphorylation (oxphos) takes place within the IM.25,48,49 Because of the crucial importance of oxphos in producing cellular ATP, which is also essential for apoptosis signaling (apoptosome formation, see below), genuine apoptotic stimuli normally do not affect the properties of the IM. However, a number of drugs and agents are able to activate a multiprotein complex that promotes the formation of a large channel in the IM, the so-called permeability transition pore (PTP).25,42,4850 Changes in mitochondrial membrane potential—ΔΨm—the energy source of oxphos—are often observed in apoptotic cells and are interpreted to derive from the PTP opening. However, most frequently these changes occur after the initial loss of OM permeability, thereby reflecting a mixture of caspase-mediated and caspase-independent damages, including the opening of the PTP.43

The first, and likely the most important, event in the intrinsic pathway of apoptosis is the loss of OM integrity and consequent release of mitochondrial proteins. Among the various proteins that leak out of mitochondria,51,52 a few, such as cytochrome c, play a prominent role in promoting the caspase cascade of cell execution, and are cumulatively called ‘apoptogenic factors.’ There appears to be a hierarchical release of apoptogenic factors during cell death signaling, with cytochrome c, Omi/Htr2A and Smac/Diablo being released first with seemingly comparable kinetics.51 The subsequent release of AIF (apoptosis inducing factor) and endoG53,54 is linked to more severe damage of mitochondrial membranes, including IM alteration. It is important to note that a direct apoptogenic role has only been demonstrated for cytochrome c. Specifically, cytochrome c is indispensable for the activation of Apoptosis Protease Activating Factor-1 (Apaf-1) and subsequent formation of the apoptosome—the ultimate cellular ‘death machine’.54

The apoptosome works like a large platform for recruiting and facilitating the self-activation of pro-caspase-9, the apical caspase of the intrinsic pathway of apoptosis.5561 There is a clear similarity in the activation of caspase-8 by the DISC and the activation of caspase-9 by the apoptosome, in that both systems rely on large multiprotein complexes to promote local accumulation of zymogens that initiate an auto-catalytic process of caspase activation.5561 The apoptosome, however, requires additional regulatory factors to fully activate the caspase cascade. Included amongst these factors is Smac/Diablo, a protein that is able to interact with several Inhibitor of Apoptosis Proteins (IAP’s) and dislodge them from their inhibitory interaction with pro-caspase-9 and other caspases.5861 As aforementioned, Smac/Diablo is also present in mitochondria (directly attached to the OM) and is efficiently released as soon as OM integrity is perturbed following intrinsic cell death stimuli.51,59 There is an important evolutionary parallel between the action of Smac/Diablo and death genes in Drosophila.62 For instance reaper acts by sequestering IAP’s from their constitutive inhibition of pro-caspases.6366 However, it is not understood why reaper and similarly acting genes are not located in insect mitochondria, while Smac/Diablo is normally sequestered within these organelles in mammalian (and presumably other vertebrate) cells. This separation might reflect the recruitment of mitochondria in the fundamental system of cell suicide of vertebrate cells.

There is some debate regarding the apoptogenic role of some proteins that escape mitochondria during cell death, in particular AIF and Omi. Genetic defects in these proteins induce a phenotype comparable to that of mitochondrial diseases,65,6769 rather than the reduction in cell death that would be expected following ablation of a genuine pro-apoptotic action, cf. Apaf-1 knockout.70 It is thus possible that both AIF and Omi are normally required for mitochondrial homeostasis and that once OM permeability is increased, they ‘accidentally’ leave mitochondria and redistribute to other compartments. They may then contribute to some fine-tuning aspects of downstream execution. Hence, the relevance of these and other (e.g., Endo G) mitochondrial proteins to death receptor-mediated apoptosis is likely to be minimal.

In the great majority of cells (Type II cells), extracellular death signals engage mitochondria in a way that is fundamentally equivalent to the intrinsic pathway.4,7,16,35 In these cells, signals emanating from the activated DISC bifurcate into two arms, one of which directly engages mitochondria via a sequence of events that are mediated by the apical caspases (8 and 10). Whereas the major target of active caspase-8 in Type I cells is caspase-3 and other executioner caspases, caspase-8 activation is slower or less efficient in type II cells and promotes the cleavage of non-caspase substrates such as Bid, as discussed further below. While caspase-8 is being slowly activated and begins to cleave these intracellular ‘messengers’ of mitochondrial engagement, a second signaling pathway is initiated by the DISC.7,71 This pathway involves the action of RIP, a serine kinase, and perhaps other ill-defined factors that regulate a caspase-independent sequence of events that lead to vacuole-mediated cell death.7173 This is especially true when caspase-8 activation is blocked.7478 Caspase-independent death signaling is particularly evident in the presence of peptide inhibitors of caspases, such as z-VAD, and produces a cellular morphology that is comparable to autophagic cell death.74,75 A similar morphology is often described as ‘necrotic’ in TNF or Fas-mediated death of certain cell types, wherein caspase-8 is either inactivated or not engaged.77,79 This ‘necrotic’ cell death pathway is becoming an increasingly hot topic in apoptosis research, and warrants additional discussion.

VACUOLE-MEDIATED CELL DEATH (NECROSIS): THE OLDEST WAY TO CELL SUICIDE?

In many Type II cells, there appears to be a fine balance between the caspase-mediated arm of death signaling that engages mitochondria and the caspase-independent arm that promotes vacuole proliferation, with at least two points of mutual regulation. Specifically, cleavage of RIP by caspase-8 results in the inactivation of the downstream cascade that promotes vacuole proliferation, thereby shutting down the caspase-independent pathway.72 A second subtler point is that the engagement of mitochondria appears to occur prior to overt activation of caspase-8 and affects membrane lipids rather than specific protein targets.25,4345,47 In essence, caspase-independent reactions mediated by the DISC promote an alteration in the homeostasis of phosphatidylcholine (PC), the major membrane lipid in cells. This leads to a cascade of metabolic effects on other lipids, including the mitochondrial lipid cardiolipin (CL), which is crucial for mediating the pro-apoptotic action of Bid and its cleaved form, tBid. Hence, the caspase-independent arm meets the caspase-dependent arm of death receptor signaling at the level of the mitochondrial membrane lipids (Fig. 1), and proceeds to converge into effective permeabilization of the OM and perturbation of mitochondrial structure and function. In this respect, death receptor-mediated apoptosis has a double-hit way of attacking mitochondria to facilitate the release of their apoptogenic factors and thus amplify the cascade of cell execution. Therefore, OM perturbation is effectively the central cross-road at which various pathways for mammalian cell suicide intersect.

Figure 1
An overview of the extrinsic and intrinsic pathways leading to programmed cell death.

The fact that mitochondria appear to not be involved in the developmental cell death of Drosophila and C. elegans cannot be used as an argument to disqualify the pivotal role of mitochondrial membranes in vertebrate pathways of cell death.8 It may well be that the simplified systems observed in invertebrates represent either deviations or over-simplifications of older pathways that have evolved since the symbiotic events that led to the emergence of eukaryotic cells.80,81 The ancestral method of programmed cell death that is observed in unicellular organisms, such as the slime molds, involves vacuole proliferation and massive autophagic degradation80,81 with morphological features that are similar to caspase-independent, death-receptor induced cell death of mammalian cells.74,77,8285 It appears the main reason that this vacuole-mediated form of PCD has been generally qualified as ‘necrotic’ 76,77,82,85 is that these cells become positive for propodium iodide staining, which is the conventional assay of plasma-membrane integrity. Although the same staining is obtained from injured cells that undergo uncontrolled ‘classical’ necrosis (characterized by a large swelling of mitochondria and other organelles), cells that die by vacuole-mediated PCD mechanisms do not succumb to the same necrotic morphology. In particular, their mitochondria are progressively degraded by vacuoles or by autophagic organelles and they maintain oxphos function for prolonged times.76,77,82,85 Therefore, the uptake of membrane impermeable dyes like propidium iodide by mammalian cells that are dying via caspase-independent mechanisms reflects enhanced endocytosis and membrane traffic linked to vacuole proliferation. Subsequent lysis of the internalized, dye-filled endocytotic vacuoles will produce intracellular staining, even if the overall integrity of the cell membrane surface has not been compromised. In other words, the dynamics of membrane traffic will be dramatically altered in autophagic cells in comparison to cells dying from caspase-mediated mechanisms. In support of this possibility, increasing evidence shows that death receptor signaling induces enhanced endocytosis in a variety of cell types, and that caspase-8 is able to regulate some key steps in membrane traffic and receptor internalization.8689 Future studies will clarify the importance and the relevance of endocytic membrane traffic in death receptor mediated apoptosis, especially with respect to the engagement of mitochondria and their membranes.80

BID: A LINK BETWEEN THE INTRINSIC AND EXTRINSIC PATHWAYS

OM permeability and the mitochondrial pathway are crucially regulated by diverse pro- and anti-apoptotic members of the Bcl-2 family.90 The anti-apoptotic members include Bcl-2, Bcl-XL and Mcl-1, whereas the pro-apoptotic members encompass Bax and Bak, as well as the BH3-only proteins, Bid and Bim.91 In particular, mitochondria are targeted by key pro-apoptotic proteins such as Bax and Bid.25,9295 Bid is a potent pro-apoptotic protein that is normally located in the cytosol, but also shuttles through the surfaces of intra-cellular membranes due to its intrinsic lipid-interacting capacity. Upon cleavage by caspase-8, cleaved Bid (and in particular its C-terminal fragment, tBid) acquires a strong propensity to bind to mitochondria, where it promotes effective OM permeabilization and the release of apoptogenic factors.92,93,96 The mitochondrial ‘receptor’ for caspase-cleaved Bid is considered to be cardiolipin (CL), a mitochondrial lipid, or metabolites of CL.25,4446

Although it is now generally accepted that tBid constitutes the fundamental link between the DISC and mitochondria,92 some observations suggest that parallel signals could be delivered to mitochondria during death receptor-mediated apoptosis. Analysis of mitochondrial membrane permeability after ex vivo activation of Fas in murine primary tissues has indicated that OM damage leading to the initial release of cytochrome c occurs prior to overt activation of caspase-8.97 In addition, it is now clear that induction of apoptosis by caspase-8 is amplified through mitochondrial release of cytochrome c.98 In a recent paper,99 Bax was demonstrated to be absolutely required for TRAIL-induced apoptosis, whereas a second report showed that Fas-induced apoptosis is independent of Bax.100 Moreover, additional evidence indicates that the DISC produces early responses that appear to be caspase-independent.101,102 Excluding the mentioned insights regarding phospholipid metabolism, the mechanism by which alternative pathways emanating from death receptors reach mitochondria remains essentially unknown.

ADDITIONAL SUBSTRATES OF APICAL CASPASES THAT TRANSMIT DEATH SIGNALS TO MITOCHONDRIA

Besides Bid and possibly other BH3-only members of the Bcl-2 family, a few other non-caspase substrates of apical caspases have been reported to promote the engagement of mitochondria in the process of death receptor-mediated apoptosis. One recent example is BAP31, a membrane protein that is involved in the export of glycosylated proteins103 and is cleaved by caspase-8.104 BAP31 is usually considered to be an ER resident protein, but is also clearly present in the Golgi of primary tissues,105 presumably reflecting constitutive trafficking of ER-Golgi Intermediate Compartment (ERGIC) vesicles.103 Although some Golgi proteins have been recently found to be substrates of executioner caspases,106 only BAP31 appears to be a specific target of apical caspases that are activated by death receptors.103 Cleavage of the cytosolic coiled-coil domain of BAP31 induces pro-apoptotic activity that is transmitted to mitochondria and seems to involve increased membrane fission.107 This has generated the ‘two-hit model’ for caspase-8-dependent alteration of mitochondria, wherein one hit is mediated by BAP31 and the other by Bid.107 The model implies that other ERGIC-associated proteins could transmit damage to mitochondria. This is likely to be dependent on the cleaved coiled-coil domain of BAP31 recruiting similar coiled-coil proteins with membrane-tethering or fusion properties.80 Our unpublished results, however, do not sustain this possibility. These studies indicate that cleavage of BAP31 may be a late event in Fas-mediated apoptosis. Future studies will establish the precise role, if any, of BAP31 and other non-mitochondrial membrane proteins in the modulation of death receptor-mediated apoptosis.108

LIPID DEGRADATION IN APOPTOSIS SIGNALING

As was summarized in a recent review by MacEwan,109 phospholipase activation has long been considered to be involved in death receptor-mediated apoptosis. The protective effects of PLA2 inhibitors in TNF-mediated cell death in vivo are similar to the initial findings regarding Ca-dependent phospholipase A2 (cPLA2).110 Phosphorylation by stress kinases can activate cPLA2, contributing to caspase-independent lipid degradation reactions. However, cPLA2 is also known to be inactivated by caspase cleavage,109,111 thereby providing another example of cross-talk between the caspase-driven and the caspase-independent bifurcation of death receptor signaling. Several other lipid degrading enzymes have been reported to be engaged in death receptor-mediated apoptosis, including Ca-independent PLA2 (iPLA2),111,112 PC-specific PLC,113,114 phosholipase D115 and sphingomyelinases.116119 To date, none of these enzymes have been found to be indispensable for apoptosis induction, especially in vivo. Moreover, mammalian PC-PLC has yet to be identified at the molecular level. However, it is likely that multiple lipid-degrading reactions follow the initial alteration in lipid metabolism, particularly of PC (Fig. 1).80 Furthermore, various lipid degradation reactions, including that typical of sphingomyelinases, are facilitated by the enhanced traffic of endocytic vesicles and lysosomal vacuoles that is promoted by death receptor activation.88 The most upstream reactions emanating from death receptors, which then trigger the cascade of metabolic changes in membrane lipids remains to be identified.

MITOCHONDRIA AND CANCER TREATMENT

Defective apoptosis is one of the hallmarks of tumorigenicity and is implicated in multiple stages of cancer development and progression.2,14,120 Additionally, the ability of tumor cells to evade apoptosis plays a significant role in promoting resistance to conventional chemotherapy and radiation therapy.7,9,10,12,121,122 Many oncogenes that deregulate the cell cycle also trigger apoptosis to eliminate cells that are proliferating inappropriately. As mitochondria play important roles in cellular energy metabolism, free radical formation and programmed cell death, defects in mitochondrial function are suspected to contribute to the development and progression of cancer and to resistance to therapy.110,123129

The role of mitochondria in cellular energy metabolism was reported by Warburg over half a century ago and was termed the “Warburg effect.” The Warburg effect indicated that a key event in carcinogenesis is the development of an “injury” to the respiratory machinery.130 This results in compensatory increases in glycolytic ATP production to satisfy the energy needs of malignant cells. Preferential reliance on glycolysis over the more energetically efficient process of oxidative metabolism has been correlated with tumor progression in several cancer types.131 Since the initial report of the Warburg effect, a number of cancer-related mitochondrial defects have been identified.110,128,129,132 These defects include altered expression and activity of respiratory chain subunits and glycolytic enzymes, changes in oxidation of NADH-linked substrates and mutations in mitochondrial DNA. Thus, the differences in energy metabolism between normal cells and cancer cells constitute a biochemical basis for the development of therapeutic strategies that might selectively kill cancer cells in their inherently compromised respiratory state.

As mitochondria are potent integrators and coordinators of apoptotic signaling pathways, induction of apoptosis in many cell types lead to the induction of mitochondrial membrane permeabilization (MMP).40,128 MMP is the event that defines the point-of-no-return in most programmed cell death models and is subject to complex regulation by pre-mitochondrial signal transduction pathways. These pathways involve DISC-dependent and DISC-independent mechanisms, members of the Bcl-2 family of proteins and changes in the composition of mitochondrial membranes.4,25,4044,50,98,125,127,128 In response to MMP, pro-apoptotic factors are released into the cytosol to trigger the execution of cell death. Under pathologic conditions, tumor cells escape from apoptosis and/or become resistant to treatment by affecting various components of the apoptotic machinery and through the inhibition of MMP.124,125,127,129 Therefore, targeting and overcoming abnormalities in tumor cells that suppress MMP could generate a potent pro-apoptotic stimulus. Moreover, since MMP is a relatively early event in the apoptotic program, methods to detect this process can be useful in assessing the response to chemotherapy.

CONCLUSIONS AND FUTURE PROSPECTS

Death receptor signaling to mitochondria appears to be significantly more complex than was originally suggested by simple cas-pase-8 activation. Death receptors activate multiple caspase-8-dependent and caspase-8-independent pathways, some of which lead to alterations in the mitochondria. For example, death receptor induced signals modulate the activities of signal transduction molecules and regulators of mitochondria such as Bcl-2 family proteins, lead to changes in mitochondrial membrane lipid metabolism, induce intracellular membrane remodeling, and instigate MMP. Together, these events promote the release of apoptogenic factors from mitochondria. The release of these factors can also lead to diverse morphological end points. Overt activation of caspase-8, on the other hand, leads specifically to rapid changes in mitochondria that promote morphological alterations that are characteristics of apoptosis. Necrosis and autophagy can also be activated in response to death receptor signaling and provide alternative mechanisms for achieving cell death. Indeed, it is suggested that various forms of PCD are triggered in response to cellular transformation and this provides a challenge for tumor cell progression and invasion. A current hurdle in the field is the identification of death receptor signaling pathways that selectively induce the apoptosis of tumor cells. Perhaps, death receptor signaling pathways that regulate changes in mitochondrial membrane lipids and the release of apoptogenic factors can be targets for the development of therapeutic agents. Additionally, the alternative programmed cell death pathway, i.e., death-receptor induced autophagy, is not well characterized and can provide a basis for tumor-selective therapeutic targets.

Acknowledgments

The suggestions of R. Shalini are most appreciated. Work in Khosravi-Far laboratory is supported by Public Health Service grant, 1 HL080192 from the National Institute of Health, DAMD, 17-02-10298 and DAMD, 17-02-10299 from the Department of Defense and RSG 03-012-01-CCG from American Cancer Society.

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