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Transfus Med Hemother. Aug 2009; 36(4): 289–292.
Published online Jul 21, 2009. doi:  10.1159/000226092
PMCID: PMC2941835
Prospective, Paired Crossover Comparison of the in vitro Quality of Red Blood Cells Collected by the Automate for Blood Collection Device or by a Conventional Method
Stefano Fontana,a Tina Weingand,b* Andreas Maier,b and Behrouz Mansouri Taleghaniac
aBlutspendedienst SRK Bern AG, Bern, Switzerland
bRegionaler Blutspendedienst Zentralschweiz, Luzern, Switzerland
cUniversitätsklinik für Hämatologie und Hämatologisches Zentrallabor, Inselspital, Universitätsspital Bern und Universität Bern, Switzerland
* Tina Weingand Regionaler Blutspendedienst Zentralschweiz Museggstrasse 146004 Luzern, Switzerland Tel. +41 41 418 70-20, Fax +27 ; tina.weingand/at/bsd-luzern.ch
Received December 10, 2009; Accepted March 3, 2009.
Background
The prevention of the citrate shock should improve the quality of red blood cells (RBCs). We compared a conventional whole blood donation method (CONV) with a ‘Automate for Blood Collection’ (ABC), enabling a metered addition of anticoagulant and hence a correct and constant RBC-to-anticoagulant ratio throughout donation. We evaluated the performance of the ABC device and the storage quality of RBC units.
Material and Methods
The study was designed as prospective, paired crossover study with two groups of 20 donors donating first with the ABC or CONV and switching to the alternative method after 12 weeks. We measured the processing data of donations and the storage quality of RBCs on days 1, 28, and 42.
Results
ABC whole blood donations showed a slightly higher volume before and after filtration. ABC-derived RBC units revealed higher values for haematocrit, mean cellular volume, potassium and lower values for mean corpuscular haemoglobin concentration and sodium until day 42. They further showed faster glucose consumption and lactate production until day 28.
Conclusion
The ABC device is suitable for whole blood collection. The quality of the obtained RBCs is comparable to that of CONV. Avoiding the citrate shock by the described method did not improve the investigated RBC storage quality parameters.
Key Words: Whole blood donation, Citrate shock, Red blood cell storage quality
During the conventional donation procedure, whole blood flows by gravitational force in a bag previously filled with a determined volume of anticoagulant solution such as CPD. The targeted ratio of 7:1 between blood and solution is achieved only at the end of the donation. Therefore, the red blood cells (RBCs) are temporarily exposed to a lower pH due to a higher concentration of anticoagulant. This imbalance is supposed to cause lesions of RBCs already during the collection procedure, as proposed by Gibson et al. [1] more than 50 years ago. This was confirmed by Mayer and co-workers [2, 3] measuring 24-hour survival rates in vivo by means of 51Cr-stained RBCs. In the following decades the storage quality of RBCs was significantly improved by several measures, including the switch from ACD to CPD and the introduction of agitating balances. Additionally, it has been postulated that a better standardisation of the whole blood-to-anticoagulant ratio may reduce the lesion of collection [4,5,6].
The ‘Automate for Blood Collection’ (ABC, MacoPharma) is a recently developed device for whole blood donation. It comprises a resting single pan balance and a roller pump, constantly adding CPD from a satellite bag into the draw line during the whole blood donation. It prevents a high CPD concentration at the beginning of the donation, adapts the CPD dose to the collected volume, and thus may improve the quality of the stored RBCs [7].
As a result, ABC is expected to improve the quality of collected RBCs. In the context of a validation study of ABC aiming for its approval for routine use in Switzerland we compared its performance and the in vitro quality of the obtained RBC concentrates with those of a conventional collection method (CONV).
Whole Blood Donation
Collection and processing of all whole blood donations were performed at the same institution. All donors gave informed consent before inclusion in the study. Donor eligibility criteria were in keeping with the regulations of the Blood Transfusion Service of the Swiss Red Cross. 40 regular whole blood donors were assigned to either the ABC procedure (n = 20) or the CONV (n = 20). After 12 weeks, the alternative method was applied in each donor. Donors were excluded from whole blood donation and from plasma- or plateletpheresis donation procedures in the 3 months before and 6 weeks before the first study donation, respectively. Other donations were also excluded during the time of participation to the study.
During CONV whole blood was collected by gravitational flow in a quadruple-bag donation set (DQE6281LW, LST2 filter; MacoPharma, Tourcoing/Lille, France), pre-filled with 63 ml of CPD anticoagulant and automatically agitated during donation (Biomixer 315, Baxter, Düdingen, Switzerland). The ABC device uses a similar quadruple-bag donation set (DQE9281LW, LST2 filter; MacoPharma). The only differences between the two donation processes are the way and the point of time of adding the CPD. In particular, with ABC the volume of blood withdrawn is continuously recorded by means of a resting (instead of agitating) single-pan balance. During the whole ABC donation, the required anticoagulant solution is added from a satellite bag containing 80 ml CPD into the draw line using a roller pump. The addition of CPD is continuously adapted to the increase of the registered weight of the drawn whole blood to achieve a constant ratio of 1:7 [7].
For CONV and ABC the obtained whole blood (450 ml) was subsequently leucocyte-filtered. The filtered whole blood was centrifuged for 18 min and 4,000 RPM (i.e. 5,134 × g) at 22 °C (Heraeus Cryo 8500 / 8000; Heraeus, Lachen, Switzerland) and separated in a RBC concentrate and a plasma unit (Compomat G4;Fresenius, Stans, Switzerland). The obtained RBC concentrate was stored in 100 ml SAG-M at 4 ± 2 °C. The plasma was frozen and not further investigated in the context of this study.
Quality Control Testing
Before donation, blood samples were drawn from each donor for a blood count (Medonic CA 620; Boule Medical AB, Stockholm, Sweden). The durations of donation and of filtration, the weight of the whole blood donation before and after filtration and those of the resulting RBC concentrates were documented. The corresponding volumes were calculated assuming a specific weight of whole blood of 1.05, of RBC concentrate of 1.07, and of CPD of 1.01. Residual white blood cell (WBC) count was performed by flow cytometry (FACSCalibur; Becton Dickinson, Basel, Switzerland) with propidium iodide staining and fluorescence beads (LeucoCOUNT kit; Becton Dickinson), according to the manufacturer's instructions. The RBC concentrates obtained from all 80 donations were analyzed on days 1, 28, and 42 of storage for the following laboratory parameters: complete blood count (ADVIA 120; Bayer Diagnostics GmbH, Munich, Germany), photometric free haemoglobin (HB) (Philips PU 8675VIS; Philips, Zurich, Switzerland), pH (at 37 °C), lactate, glucose, potassium (K+) and sodium (Na+) (ABL 700 Radiometer; Radiometer, Copenhagen, Denmark). The rate of haemolysis was calculated with the following formula:
equation m1
(1)
The haematocrit (HC) is expressed in %, the free and total HB in g/l.
Statistical Analysis
All data were analysed with computer software (NCSS, Kaysville, UT, USA). Donation, filtration, and further quality control data were compared with the t-test for paired samples (parametric data) or the Wil-coxon signed-rank test for non-parametric data. In view of multiple parameters compared, a significant difference between groups was assumed by a more restrictive p value of <0.01 in a two-tailed test.
Donor Characteristics, Donation and Filtration Data
All 40 enrolled donors completed the study. All resulting 80 donations were evaluable and yielded RBC concentrates meeting current Swiss quality standards. The donor population consisted of 13 females and 27 males and had a mean age of 43 (range 19–65) years. The mean HB values before donation were 155 (129–179) g/l for CONV and 156 (range 133–174) g/l for ABC (p = 0.77).
The donation and filtration data are summarised in table table1.1. We observed a statistically significant, but minimal volume difference between the ABC and CONV products before and after filtration. There was no difference in the loss of volume during the procedures. The smaller standard deviations of the volumes of ABC collections imply a better standardisation of the collected volume. The WBC contamination was higher in the products obtained by ABC, but all showed a WBC count well below 106 per unit.
Table 1
Table 1
Donation and filtration data
Quality Control Data at Days 1, 28, and 42
The results of the analyses performed during storage at days 1, 28, and 42 are presented in table table2.2. They show several small differences between the two collection procedures. Compared to CONV, the RBCs obtained by the ABC system showed a higher HC and mean corpuscular haemoglobin concentration (MCHC) during the complete storage time as well as an increasing mean cellular volume (MCV) difference until day 42. Electrolyte changes comprise a faster increase of K+ and a decrease of Na+. Metabolic changes are characterised by faster glucose consumption, lactate production, and an accelerated but not significant decline of pH between day 1 and day 28.
Table 2
Table 2
Quality control data at days 1, 28, and 42
Modern whole blood collecting systems and additive solutions allow for storage of RBC up to 42 or 49 days with acceptable in vivo survival rates [8, 9].
However, many adverse changes in stored RBCs may impair the oxygen transport and delivery capacity of the transfused RBCs, like the loss of S-nitrosohaemoglobin [10] or of 2,3-DPG [11]. These alterations are known to occur during processing and storage of RBCs and are related to storage conditions such as temperature, pH, and chemical composition of the environment. Approved quality assessments of RBCs at the end of their storage duration comprise the HB content, the rate of haemolysis and the residual leucocyte count [12]. For our study we extended these quality control parameters as described above.
The blood collection and conservation systems have been steadily optimised, taking many of these aspects into account. Some factors may influence the quality of RBCs already during the collection process itself, like an overwhelming concentration of anticoagulant during the first minutes of blood donation or a variable whole blood-to-anticoagulant ratio [4, 5]. During a conventional donation procedure, whole blood flows in a bag previously filled with a prefixed volume of CPD anticoagulant solution. The targeted ratio of 7:1 between blood and solution is achieved only at the end of the donation. Therefore, the RBCs are initially exposed to a high concentration of anticoagulant and an accordingly low pH for a few minutes. This may result in a ‘lesion of collection’. For RBCs being held in ACD for up to 30 min, it has been shown that their in vitro quality and their in vivo 24-hour survival rates are impaired [1,2,3,4].
The ABC may improve the quality of collected RBCs by continuously adding a metered volume of CPD from a satellite bag into the draw line during the whole blood donation [6]. Limited data about ABC have been reported since 2003. These were restricted to processing data [13,14,15] or, if additional quality control analyses of RBCs were performed, were not directly compared with results of already validated systems [15, 16]. A recently published controlled study focused on quality of RBC concentrates of sickle trait donors, but the number of donations is insufficient to infer on the quality of ABC under normal conditions [17]. We present the first study comparing the ABC device with an approved standard whole blood collection system, which was otherwise virtually identical and manufactured by the same company. The comparability of the two groups is further guaranteed by the equal predonation HB values and the crossover design.
When comparing the performance of CONV and ABC methods for blood donation and processing, both showed identical values for donation and filtration times as well as for loss of volume by filtration. Although the number of residual WBCs in ABC is higher than in CONV, all products show WBC counts clearly below 106 per unit. The higher volume of the ABC donations, partially due to the slightly higher volume of CPD anticoagulant, persists after filtration. The lower standard deviations additionally indicate a more standardised collection volume with ABC compared to CONV. This may be the result of continuously weighing a resting instead of an agitating bag. However, this does not result in a higher standardisation of the final products (RBC units and plasma), as measured by the standard deviations of the quality control data during storage (table (table22).
All quality control parameters meet the current Swiss and European standards. The majority of the haematological and chemical changes observed during storage of RBCs obtained by ABC or CONV are comparable. We only observed some minor differences in several parameters. Although the identical centrifugation and separation protocols of ABC and CONV lead to identical total HB contents in the RBC units, an increase of MCV resulted in an increased HC and a decreased MCHC in ABC. The latter has also been reported for RBCs derived from erythroplasmapheresis with a single needle apheresis device [6]. With respect to the discordant course of K+ and Na+ concentrations, enhanced physical changes in the RBC membrane collected with ABC have to be suggested, e.g. by an impaired activity of the Na-K-ATPase. Additionally, we observed higher glucose consumption and corresponding increased lactate concentration between day 1 and 28 in RBCs collected by ABC. These differences were too small to result in significantly different pH values, and disappeared until day 42. More in-depth in vitro analyses (such as 2,3-DPG, rheological parameters, or even proteomics) and clinical outcome parameters may be included in future studies.
Taken together our results revealed the ABC device to be suitable for whole blood collection. The quality of the obtained RBCs is comparable to that of products gained by conventional whole blood donation methods and meets the current Swiss and European standards. Avoiding the citrate shock by maintaining a correct and constant whole blood-to-anticoagulant ratio during donation with the described method at its current stage of development did not improve the investigated RBC storage quality parameters.
Disclosure
The authors declared no conflict of interest.
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