The expression of activation markers and costimulatory molecules on splenic PD-1+ T cells from aged mice
Single cell suspensions from spleens were stained for the surface markers CD4, CD8, CD25, CD28, CD69, CD127, CTLA-4, ICOS and PD-1 and intracellularly for CTLA-4, and ICOS. Expression of PD-1 versus CD69 on CD4+ and CD8+ T cells from young (3-month old) and aged (16-month old) mice (A). Percentage of cells in PD-1+CD4+ (B) and PD-1+CD8+ (C) T cells expressing the different listed markers are shown. Significance was considered for p values less than 0.05 and indicated with asterisks.