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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
Sci Signal. Author manuscript; available in PMC 2010 September 17.
Published in final edited form as:
PMCID: PMC2941205

The Nonphagocytic NADPH Oxidase Duox1 Mediates a Positive Feedback Loop During T Cell Receptor Signaling


Production of reactive oxygen species, often by NADPH (reduced form of nicotinamide adenine dinucleotide phosphate) oxidases, plays a role in the signaling responses of cells to many receptor stimuli. Here, we describe the function of the calcium-dependent, nonphagocytic NADPH oxidase Duox1 in primary human CD4+ T cells and cultured T cell lines. Duox1 bound to inositol 1,4,5-trisphosphate receptor 1 and was required for early T cell receptor (TCR)–stimulated production of hydrogen peroxide (H2O2) through a pathway that was dependent on TCR-proximal kinases. Transient or stable knockdown of Duox1 inhibited TCR signaling, especially phosphorylation of tyrosine-319 of ζ chain–associated protein kinase of 70 kilodaltons (ZAP-70), store-operated entry of calcium ions (Ca2+), and activation of extracellular signal–regulated kinase. The production of cytokines was also inhibited by knockdown of Duox1. Duox1-mediated inactivation of Src homology 2 domain–containing protein tyrosine phosphatase 2 promoted the phosphorylation of ZAP-70 and its association with the Src family tyrosine kinase Lck and the CD3ζ chain of the TCR complex. Thus, we suggest that activation of Duox1, downstream of proximal TCR signals, generates H2O2 that acts in a positive feedback loop to enhance and sustain further TCR signaling.


Receptor stimulation by multiple mediators, such as those for platelet-derived growth factor (1), insulin (2), or angiotensin II (3), induces the production in cells of reactive oxygen species (ROS), which control their biological functions. These ROS act as obligate second messengers, regulating protein kinase activation, gene expression, and proliferative responses. Many studies in T cells have used exposure to exogenous oxidants, such as hydrogen peroxide (H2O2), to mimic or enhance signaling through the T cell receptor (TCR) (46). Studies have shown that TCR-induced signaling is regulated by receptor-mediated production of ROS (7), although the sources of ROS and their mechanisms of action remain unclear.

TCR signaling involves a complex array of protein kinases, phosphatases, phospholipases, and adaptor proteins (8). Engagement of the TCR with antigen induces the activation of the Src family tyrosine kinase Lck, which phosphorylates immunoreceptor tyrosine-based activation motifs (ITAMs) on the ζ chain homodimers and CD3 chains of the TCR complex. The dually phosphorylated ITAMs create binding sites for the Src homology 2 (SH2) domains of the Syk family tyrosine kinase, ζ chain–associated protein kinase of 70 kD (ZAP-70), which in combination with Lck phosphorylates key early mediators of TCR signaling, including the adaptor proteins linker of activated T cells (LAT) and SH2 domain–containing leukocyte phosphoprotein of 76 kD (SLP-76). Inhibition of these proximal signals can occur at several levels. For example, C-terminal Src kinase (Csk), which is recruited to the membrane by the adaptor protein Csk-binding protein (Cbp, also known as PAG), phosphorylates Lck at its inhibitory site, Tyr505 (Y505) (9). Alternatively, dephosphorylation of Tyr394 (Y394) in the activation loop of Lck by the protein tyrosine phosphatase (PTP) CD45 is proposed to inhibit proximal TCR signals (10). Similarly, other PTPs including SH2 domain–containing PTP–2 (SHP-2) (11) and SHP-1 (12, 13) are thought to inhibit early TCR signaling.

In terms of downstream TCR effector signals, once the appropriate tyrosines are phosphorylated, LAT serves as a scaffold to recruit the adaptor proteins Grb2 and Gads, as well as phospholipase C–γ1(PLC-γ1) (14). Gads links with SLP-76 and brings it in proximity to LAT (15), controlling cytoskeletal changes and adhesion, whereas recruitment of Grb2 leads to activation of the Ras–mitogen-activated protein kinase (MAPK) pathway. The activity of PLC-γ1 induces increases in the concentration of cytoplasmic calcium ions (Ca2+) through the production of inositol 1,4,5-trisphosphate (IP3), which, upon binding to IP3 receptor 1 (IP3R1), releases Ca2+ from intracellular stores and activates subsequent capacitive Ca2+ entry through plasma membrane channels (16). These signals lead to the activation of transcription factors such as the Ca2+-dependent nuclear factor of activated T cells (NFAT) and the MAPK-mediated activating protein–1 (AP-1), which initiate the expression of important regulatory genes that control cellular proliferation, differentiation, and cell survival, including interleukin-2 (IL-2) (17).

Some studies have focused on mitochondria as a source of early TCR-stimulated ROS. One report described impairment of calcium-dependent production of ROS in T cells from mice lacking expression of the genes encoding the BH3 Bcl-2 family members Bax and Bak (18), whereas another report showed that inhibitors of mitochondrial function or deletion of mitochondrial DNA inhibited TCR-induced generation of ROS in a model of activation-induced cell death (19). These data are consistent with other findings that link mitochondrial metabolic activity with the generation of ROS in activated T cells (20). Other studies, however, point to potential roles of nonmitochondrial sources of ROS in activated lymphocytes, including NADPH (reduced form of nicotinamide adenine dinucleotide phosphate) oxidases (21) and lipoxygenases (22). Investigation of the potential sources of ROS in many cell types has also identified the phagocyte-type NADPH oxidase and a family of homologs of the catalytic subunit of the oxidase (Nox and Duox proteins) that are found in nonphagocytic cells (23). Whereas the activation of oxidases in the respiratory burst of phagocytes leads to the generation of increased amounts of the superoxide anion, in nonphagocytic cells the oxidases produce less ROS, which are not cytotoxic, but regulatory. Thus, receptor signaling induces the generation of ROS from a family of NADPH oxidases that modulate cellular signaling and function.

The roles of ROS and NADPH oxidases in the regulation of T cell function are not clear and may involve several oxidases. We previously demonstrated that T cells have functional phagocyte-type NADPH oxidase (Nox2) (21). Compared to T cells from wild-type mice, T cells from Nox2-deficient mice exhibit defective production of H2O2, enhanced activation of extracellular signal–regulated kinase (ERK), and a relative increase in the amounts of secreted T helper 1 (TH1)–type cytokines. Thus, mature T cells have a functional phagocyte-type NADPH oxidase that appears to inhibit some elements of TCR signaling; however, an early phase of H2O2 generation in T cells is independent of Nox2. This generation of H2O2 occurs within 2 to 4 min of TCR stimulation and is consistent with the kinetics of early TCR signaling and TCR-induced oxidation of PTPs, as described previously (24). Thus, the goal of the current study was to determine the sources of this early TCR-stimulated H2O2 and its role in TCR signaling.

Here, we demonstrate that Duox1, a Ca2+-dependent nonphagocytic NADPH oxidase, is responsible for early TCR-stimulated generation of H2O2. Activation of Duox1 was dependent on early tyrosine kinase signaling, PLC-γ1, and the release of Ca2+ from the endoplasmic reticulum (ER). Knockdown of Duox1 in primary human CD4+ T cells and Jurkat cells (a human CD4+ T cell leukemia cell line) selectively inhibited the generation of H2O2 and the phosphorylation of proximal signaling molecules, suggesting a positive role for Duox1 in TCR signal transduction. Specifically, phosphorylation of Tyr319 (Y319) in ZAP-70 and activation of ERK were inhibited by knockdown of Duox1, and these effects were reversed upon reconstitution of cells with Duox1. Duox1 was coimmunoprecipitated with IP3R1, and knockdown of Duox1 inhibited store-operated influx of Ca2+, suggesting a role for Duox1-derived H2O2 in the regulation of Ca2+ flux. Consistent with our previous studies (24), TCR stimulation induced the oxidation of the PTP SHP-2, which was diminished in T cells with stable knockdown of Duox1. These data support a model in which Duox1-mediated oxidation of SHP-2 enhances proximal TCR signaling through regulation of the phosphorylation and activation of ZAP-70. Thus, the results suggest that Duox1-mediated production of H2O2, which is dependent on proximal TCR signal pathways, is a key mediator of TCR signaling that acts in a positive feedback loop to promote proximal and downstream TCR signal transduction.


Signals that regulate the early generation of ROS

In Jurkat cells, cross-linking (stimulation) of the TCR complex with antibody against CD3 (OKT3) induced the generation of ROS, as measured by the increased fluorescence of the oxidation-sensitive dye dichlorodihydrofluorescein diacetate (DCFDA) within 2 to 4 min of stimulation, which returned to baseline after ~15 min (Fig. 1A) (25). Our previous data in T cell blasts suggested that this early phase of ROS generation is independent of the phagocyte NADPH oxidase (Nox2) (21), which only affected a later phase (30 min) of DCFDA oxidation; however, our current studies suggested potential roles for another NADPH oxidase in early TCR-stimulated generation of ROS. All subsequent experiments monitored this early phase of ROS generation by measuring the amount of oxidized DCFDA present 10 or 15 min after TCR cross-linking (26). Some NADPH oxidases, such as Nox1, Nox2, and Nox3, are dependent on the small guanosine triphosphatase (GTPase) Rac1, whereas others, such as Nox4, Nox5, Duox1, and Duox2, may not require Rac1 (2729). Expression of a dominant-negative (DN) Rac1 (N17Rac1) did not inhibit early TCR-induced oxidation of DCFDA (fig. S1A). Oxidation of DCFDA was sensitive to the general flavin electron transfer inhibitor diphenylene iodonium (DPI) (fig. S1B). Thus, these data are consistent with a Rac-independent NADPH oxidase as a potential source of ROS early after stimulation of the TCR.

Fig. 1
Signaling pathways that regulate the early-phase generation of ROS in TCR-stimulated Jurkat cells. (A) Kinetics of TCR-induced generation of ROS in Jurkat cells detected within the indicated intervals by measurement of the oxidation of DCFDA (open circles) ...

To further delineate biochemically the signaling steps required for the early TCR-induced generation of ROS, we manipulated early TCR signals with pharmacologic and molecular tools. Consistent with previous studies (19), Jurkat cell lines deficient in Lck or ZAP-70 did not exhibit TCR-induced generation of ROS (Fig. 1B). The TCR-induced increase in the concentration of intracellular Ca2+ is controlled through PLC-γ1–mediated production of IP3 and its binding to IP3R1. Jurkat cells deficient in PLC-γ1 (J.gamma1) (30) or IP3R1 (IP3R1-deficient) (31) generated substantially less ROS upon TCR stimulation than did control cells (Fig. 1C). Reconstitution of J.gamma1 cells with wild-type PLC-γ1 (to generate J.gamma1.WT cells) partially restored the oxidation of DCFDA in response to TCR stimulation. Furthermore, transfection of Jurkat cells with a plasmid encoding a DN mutant (Y783F) of PLC-γ1(32) inhibited the generation of ROS (Fig. 1D). Consistent with the importance of PLC-γ1 in this process, stable Jurkat cell clones that had mutations in the adaptor protein SLP-76 that prevented SLP-76 from associating with or activating PLC-γ1(Y3Fand ΔGads, respectively) (33) showed a marked decrease in the extent of TCR-induced generation of ROS relative to that of cells transfected with a plasmid encoding either a full-length or a variant SLP-76 with a mutation in the SH2 domain that prevents its association with adhesion- and degranulation-promoting adaptor protein (ADAP) (mSH2) (Fig. 1E). Furthermore, chelating intra-cellular Ca2+ with BAPTA [1,2-bis(o-aminophenoxy) ethane-N, N, N′, N′-tetraacetic acid] inhibited the early generation of ROS in Jurkat cells (Fig. 1F). Together, these data are suggestive of a role for intracellular Ca2+ in controlling the generation of ROS; however, extracellular Ca2+ was not required for the oxidation of DCFDA, as determined in the presence of EGTA or upon stimulation of cells in Ca2+-free buffer (Fig. 1F). Thus, mechanisms that control TCR-induced increases in the release of Ca2+ from intracellular stores are required for the early generation of ROS, and the data are consistent with a role for a Ca2+-dependent NADPH oxidase that is DPI-sensitive but that does not require Rac1.

Ca2+ flux alone was not sufficient for the generation of ROS because exposure to thapsigargin (Fig. 2A) or ionomycin (Fig. 2B), which increases the concentration of intracellular Ca2+, in the absence of TCR stimulation did not induce the oxidation of DCFDA. Co-incubation of cells with either drug during TCR stimulation did not markedly augment the extent of DCFDA oxidation when compared with that in TCR-stimulated cells in the absence of drug. The addition of ionomycin or thapsigargin to PLC-γ1–deficient Jurkat cells restored TCR-induced oxidation of DCFDA to an extent similar to that observed in cells in which PLC-γ1 was restored (Fig. 2, A and B). Thus, these data suggest that TCR-stimulated release of Ca2+ from intracellular stores is necessary, but not sufficient, for the early generation of ROS, and that other elements of TCR signaling are required.

Fig. 2
Activation of the NADPH oxidase Duox1 in TCR-stimulated Jurkat cells. (A and B) Ca2+-mobilizing agents alone do not trigger the early-phase generation of ROS. TCR-induced oxidation of DCFDA was measured 10 min after TCR stimulation of Jurkat E6-1 cells ...

Expression of Duox1 messenger RNA and protein in human T cells

Given that the TCR-dependent generation of ROS was Ca2+-dependent, Rac1-independent, and sensitive to DPI, we considered Duox1 and Duox2 as potential sources of ROS. Reverse transcription polymerase chain reaction (RT-PCR) analysis revealed the presence of messenger RNAs (mRNAs) for each of these oxidases in Jurkat cells and primary human CD4+ T cell blasts, which was confirmed by cloning and sequencing of the PCR products (fig. S2, A and B). Duox1 and Duox2 proteins were detected in Jurkat cells by Western blotting analysis (Fig. 2C). When examining the mobility of Duox1 protein by SDS–polyacrylamide gel electrophoresis (SDS-PAGE), we found that TCR stimulation caused a shift in the mobility of Duox1 that was insensitive to dithiothreitol (DTT) (Fig. 2C). This occurred within 5 min of TCR stimulation and was reversed within 60 min, suggesting a post-translational modification, such as phosphorylation, during Duox1 activation. The kinetics of the mobility shift in Duox1 paralleled a similar shift in Lck, which is thought to be due to phosphorylation of a serine residue (25). The mobility of Duox2 was affected neither by TCR stimulation nor by DTT. Further analysis showed that stimulation of Jurkat cells with the phorbol ester PMA (phorbol 12-myristate 13-acetate) also induced a mobility shift in Duox1 protein (Fig. 2D). These data are suggestive of the phosphorylation of Duox1 upon TCR stimulation. Western blotting analysis showed that immunoprecipitated Duox1 was detected with either a monoclonal antibody against phosphoserine (pSer) residues or an antibody against substrates of protein kinase C (PKC) that recognizes a pSer in the consensus sequence (R/K)XS(Hyd)(R/K) (Fig. 2E). These data are consistent with earlier results (Fig. 2, A and B), which suggest that a TCR-induced signal in addition to Ca2+ flux is required for the activation of Duox1. In addition, the data agree with a previous report that Nox5, one of the Ca2+-dependent members of the Nox family, undergoes serine phosphorylation, which enhances its responsiveness to lower concentrations of Ca2+ (34).

Effects of suppressed expression of Duox1 on TCR signaling in Jurkat cells

Commercially available small interfering RNAs (siRNAs) were analyzed for their ability to inhibit the expression of Duox1 or Duox2 transcripts (fig. S2C) in Jurkat cells, which were transfected with nontargeting (NT) siRNAs as a control. Duox1-specific, but not Duox2-specific, siRNAs substantially inhibited early (10 min) oxidation of DCFDA after TCR stimulation (fig. S2D). Thus, these data suggest that Duox1 was responsible for early TCR-stimulated generation of ROS. To further examine the role of Duox1 in TCR signaling, we isolated stable clones of Jurkat cells after transfection with either a plasmid encoding short hairpin RNA (shRNA) specific for Duox1 or an NT vector (NT cells). The abundance of Duox1 protein was substantially reduced in the Duox1-targeted cell clone (Fig. 3A), whereas the abundance of Duox2 protein was unaffected. In addition, the abundance of the phagocytic oxidase Nox2 was also unaffected by knockdown of Duox1. TCR-stimulated early oxidation of DCFDA, which occurred within 10 or 15 min in NT cells, was almost completely absent in the Duox1-targeted cells (Fig. 3B). At longer time points, however, there was still measurable generation of ROS, although it was substantially decreased compared to that in the NT cells (Fig. 3B). Together, these data are consistent with the presence of functional Duox1 in T cells and its role in early TCR-stimulated generation of ROS.

Fig. 3
Effects of the knockdown of Duox1 on TCR signaling in Jurkat cells. (A) Analysis of the abundances of Duox1, Duox2, and Nox2 proteins in Jurkat cell clones expressing NT or Duox1-specific (Duox1) shRNAs. The graph represents the average band density of ...

In experiments with the stable Duox1-knockdown clone of Jurkat cells, we investigated the effects of early generation of H2O2 on TCR signaling (Fig. 3, C to E). Downstream functional consequences of the signaling changes caused by knockdown of Duox1 were measured with luciferase-based reporter constructs for the transcription factors AP-1 and NFAT. TCR-induced activation of both reporter constructs was substantially inhibited in the Duox1-knockdown cells compared to that in the NT cells (Fig. 3C). Thus, Duox1 was also required for optimal TCR-induced activation of AP-1 and NFAT, which suggests that Duox1 is needed for the activation of ERK, which regulates AP-1 activation, and for the increase of intracellular Ca2+ concentrations, which is required for the nuclear translocation of NFAT.

Knockdown of Duox1 inhibited the TCR-induced phosphorylation of ERK1 and ERK2 and that of the Syk family kinase ZAP-70 at Tyr319,suggesting that Duox1-dependent generation of H2O2 plays a positive role in their activation (Fig. 3D). Phosphorylation of Tyr319 in ZAP-70 is thought to be catalyzed by Lck or Abl (35) and is critical for the activation of ZAP-70 and of downstream signaling pathways, including the ERK pathway (36). However, phosphorylation of the inhibitory residue Tyr505 in Lck was markedly diminished by knockdown of Duox1, whereas phosphorylation of the positive regulatory site Tyr394 was not substantially affected (Fig. 3E). Together, these results suggest that Duox1-derived H2O2 may inhibit the activity of Lck by augmenting the extent of phosphorylation of Tyr505.Furthermore, inhibition of the kinase activity of ZAP-70 did not appear to be a result of the decreased activation of Lck.

To validate the effects of knockdown of Duox1 on proximal TCR signaling in Jurkat cells, we transfected Duox1-knockdown cells with a plasmid encoding Duox1. Reconstitution of Duox1-knockdown cells with Duox1 reversed the phenotype, leading to the increased phosphorylation of ERK1 and ERK2, Tyr319 of ZAP-70, and Tyr505 of Lck (Fig. 4A). Consistent with these observations, transfection of Jurkat cells with plasmid encoding Duox1 also augmented TCR-stimulated phosphorylation of ERK1, ERK2, and Tyr319 of ZAP-70 compared to that in control, untransfected cells (Fig. 4B). Conversely, transfection with plasmid encoding Duox2 did not augment, and even slightly inhibited, the activation of ERK (Fig. 4C).

Fig. 4
Effects of the increased abundance of Duox1 on TCR signaling in Jurkat cells. (A) The Duox1-knockdown Jurkat cell clone was transfected with an empty vector or with a plasmid encoding HA-tagged Duox1. TCR-induced phosphorylation of ZAP-70 (Y319), ERK1 ...

Involvement of Duox1 in TCR-mediated Ca2+ signaling

We assessed the effects of Duox1 on intracellular Ca2+ mobilization because Ca2+ release from intracellular stores is necessary for Duox1-dependent early generation of H2O2, and knockdown of Duox1 inhibited TCR-induced activation of NFAT. In the context of TCR stimulation, knockdown of Duox1 strongly inhibited sustained increases in intracellular Ca2+ in the presence of extracellular Ca2+, which was driven mainly by Ca2+ influx from extracellular sources (Fig. 5A). Decreased sustained intracellular Ca2+ concentrations were also observed in the presence of ionomycin, although the effect was milder. These strong inhibitory effects of knockdown of Duox1 were not observed in the absence of extracellular Ca2+, further suggesting that upon TCR stimulation, Duox1-mediated generation of H2O2 stimulates the entry of Ca2+ through store-operated Ca2+ channels. Thus, these data suggest that Duox1-dependent generation of H2O2 is important for the Ca2+-dependent immune response. Further support of the involvement of Duox1 in intracellular Ca2+ signaling was found by coimmunoprecipitation of IP3R1 with Duox1 in Jurkat cells (Fig. 5B). In human embryonic kidney (HEK) 293 cells stably expressing either Duox1 or Duox2, IP3R1 was found only in samples immunoprecipitated with an antibody against Duox1 and was not detected in samples associated with Duox2 (Fig. 5C), even though both proteins were highly abundant. Thapsigargin did not change the association between Duox1 and IP3R1. Thus, the data further support a role for Duox1 in regulating proximal TCR signaling, especially its interaction with pathways of Ca2+ signaling.

Fig. 5
Duox1-mediated regulation of intracellular Ca2+.(A) TCR-induced Ca2+ flux in Jurkat cell clones stably transfected with NT (dotted lines) or Duox1-specific (solid lines) shRNAs. Cells were loaded with Fluo-3 and Fura Red, stimulated with ionomycin or ...

Effect of knockdown of Duox1 on TCR signaling in CD4+ T cell blasts

Transient transfection of primary human CD4+ T cell blasts with the Duox1-specific siRNA, which showed the same effects that we observed in Jurkat cells, resulted in the decreased abundance of Duox1 protein, with little effect on the abundance of Duox2 protein (Fig. 6A). TCR-stimulated oxidation of DCFDA was also inhibited by the Duox1-targeting siRNA (Fig. 6B). Thus, these data support a role for Duox1 in early TCR-stimulated generation of ROS in primary T cells as well as in the Jurkat cell line. We compared TCR-induced tyrosine phosphorylation in activated human CD4+ T cells that had been transfected with NT siRNA or Duox1-specific siRNA (Fig. 6C). Western blotting analysis of whole-cell lysates showed that the extent of tyrosine phosphorylation of Lck, ZAP-70, TCRζ, and the adaptor molecule LAT was substantially decreased in Duox1-knockdown cells compared to that in control cells.

Fig. 6
Effects of knockdown of Duox1 on TCR signaling in CD4+ human T cell blasts. CD4+ human T cell blasts were transfected with NT or Duox1-specific siRNAs and cultured for 40 hours. (A) Effects of NT or different Duox1-specific siRNAs (Duox1-2 or Duox1-3) ...

We also assessed the effects of knockdown of Duox1 on the activation of specific signaling molecules. Knockdown of Duox1 caused a substantial decrease in the extent of TCR-stimulated phosphorylation of Tyr319 of ZAP-70 and of Tyr783 of PLC-γ1 (Fig. 6D). To examine the functional relevance of Duox1-derived H2O2 in the T cell blasts, we measured TCR-induced production of cytokines. Our previous study showed that Nox2-deficient murine CD4+ T cells had a TH1-skewed cytokine profile (21). In contrast, knockdown of Duox1 led to a general decrease in the amount of secreted cytokines without an obvious skewing to a TH1- or TH2-type profile. TCR-induced secretion of IL-4, IL-10, and interferon-γ (IFN-γ) by Duox1-knockdown cells was diminished by ~40% compared to that of control cells, whereas the secretion of other cytokines, such as IL-8 and RANTES, was unaffected (Fig. 6E).

Consistent with the effects of knockdown of Duox1 in Jurkat cells, knockdown of Duox1 in CD4+ T cell blasts led to the diminished phosphorylation of ERK compared to that in control CD4+ T cells (Fig. 7A). Our previous data suggested that the phosphorylation of the guanine nucleotide exchange factor Vav1 and the SLP-76–associated adaptor protein ADAP was regulated by TCR-induced early generation of ROS (24). Indeed, knockdown of Duox1 inhibited the phosphorylation of Tyr174 of Vav1, a key step for its activation (Fig. 7A) (37). The adaptor protein Gads recruits ADAP to LAT through SLP-76, whereas Grb2 is another critical adaptor that recruits proteins to LAT. To see the effects of knockdown of Duox1 on specific TCR-proximal phosphoproteins associated with these adaptors, we serially immunoprecipitated Gads and Grb2 from human T cells transfected with NT siRNA or Duox1-specific siRNA. Knockdown of Duox1 decreased the extent of TCR-induced phosphorylation of ADAP associated with immunoprecipitated Gads (Fig. 7B). More tyrosine-phosphorylated proteins were associated with Grb2 than with Gads (Fig. 7B), and knockdown of Duox1 markedly inhibited the extent of tyrosine phosphorylation of TCR-proximal signaling molecules, such as PLC-γ1, Lck, and LAT, which were coimmunoprecipitated with Grb2 or Gads (Fig. 7, B and C).

Fig. 7
Effects of knockdown of Duox1 on TCR-stimulated activation of ERK and signaling phosphoproteins associated with the adaptor proteins Gads and Grb2. CD4+ human T cell blasts were transfected with NT or Duox1-specific siRNAs as described for Fig. 6. (A ...

Thus, in primary T cell blasts, Duox1 appears to promote proximal TCR signaling, especially the phosphorylation of ZAP-70, PLC-γ1, Vav1, and ADAP, as well as the activation of ERK1 and ERK2. The Grb2-associated adaptor protein Gab2 is recruited to TCR-proximal signaling complexes through interactions with Grb2 and Gads in Jurkat cells (38). Our data showed that in human T blasts, most of Gab2 was associated with Grb2 in serial immunoprecipitations with antibodies against Gads and then Grb2 (Fig. 7B). Knockdown of Duox1 decreased the extent of phosphorylation of Gab2 and diminished its association with SHP-2 in the Grb2 immunoprecipitates (Fig. 7C).

SHP-2 associates with Gab2 and inhibits proximal TCR signaling (11). Consistent with these findings, we found that TCR stimulation increased the association between SHP-2 and Gab2, and knockdown of Duox1 abrogated the TCR-induced increase in their association in primary CD4+ T cell blasts (Fig. 8A). Similarly, transfection of cells with a plasmid encoding an inactive mutant of SHP-2 [SHP-2 (C/S)], which mimics an increased pool of SHP-2 inactivated by Duox1-derived H2O2, increased the association of SHP-2 with Gab2, suggesting that inactivation of SHP-2 affects its association with the TCR-proximal signaling complex (Fig. 8B). The interaction between Gab2 and SHP-2 is proposed to bring SHP-2 in proximity to the plasma membrane and into the proximal TCR signaling complex (38). Our previous study demonstrated that TCR-mediated early generation of ROS induced the inactivation of SHP-2 through the oxidation of its active-site cysteine (24). Thus, we focused on the potential regulation of SHP-2 by Duox1. The oxidation of the active-site cysteine residue of SHP-2 was analyzed with an antibody (oxPTP) that recognizes the sulfonic acid form of cysteine on the active-site signature motif of a PTP (39). Because the adaptor Gab2 is expected to bring SHP-2 to the membrane where the NADPH oxidase resides, we analyzed the oxidation state of SHP-2 in cytosolic and membrane fractions (Fig. 8C). TCR stimulation increased the extent of oxidation of the active-site cysteine in SHP-2, but mainly in the membrane fraction, and this was inhibited in the Duox1-knockdown cells (Fig. 8C). Thus, the data suggest that Duox1-derived H2O2 oxidizes the active-site cysteine of SHP-2. Furthermore, these results also suggest that the oxidation of SHP-2 occurs primarily near the plasma membrane, potentially in association with Gab2. The observations in Duox1-knockdown cells are consistent with a model in which SHP-2 is more active and efficiently dephosphorylates target substrates in the absence of H2O2-mediated inactivation. Such targets would include binding sites on Gab2 for the SH2 domains of SHP-2, which is supported by the decreased association of SHP-2 with Grb2 (Fig. 7C) and Gab2 (Fig. 8A). Furthermore, the opposite effect was observed in cells transfected with plasmid encoding the inactive SHP-2 mutant, SHP-2 (C/S) (Fig. 8B).

Fig. 8
Duox1-mediated redox regulation of SHP-2. (A and B) Duox1-mediated regulation of the interaction between SHP-2 and Gab2. (A) CD4+ human T cell blasts were transfected with NT or Duox1-specific siRNAs and stimulated by cross-linking of the TCR, as described ...

Because of previous studies that describe Gab2-mediated inhibitory effects on both ZAP-70 and TCRζ, we focused on how Duox1-mediated oxidation of SHP-2 regulated the potential effects of SHP-2 on ZAP-70. Phosphorylated Tyr319 destabilizes the autoinhibitory interface of ZAP-70 and stabilizes its active state (35, 40). Tyr319 also serves as an adaptor site for the SH2 domain of Lck or PLC-γ1 (36, 41), which may bring critical signaling molecules together to augment signal intensity. We transfected cells with a plasmid encoding SHP-2 (C/S) to increase the amount of inactive SHP-2, and other cells with plasmid encoding wild-type SHP-2 to increase the pool of active SHP-2. The TCR-stimulated association of ZAP-70 with TCRζ (Fig. 9A) and Lck (Fig. 9B) was enhanced in the presence of SHP-2 (C/S), as was phosphorylation of TCRζ in these complexes (Fig. 9C), which suggested that active SHP-2 inhibited the formation of a ZAP-70–Lck–TCRζ complex. Consistent with a regulatory role for SHP-2 in this complex, SHP-2 was found in Lck immunoprecipitates, and the presence of SHP-2 (C/S) increased the amount of SHP-2 associated with Lck (Fig. 9C). Knockdown of SHP-2 with specific siRNA enhanced the association between ZAP-70 and Lck in human CD4+ T cells (Fig. 9D), further supporting a role for SHP-2 in this proximal signaling complex. In contrast, knockdown of Duox1 markedly diminished the TCR-stimulated association between ZAP-70 and Lck (Fig. 9E), whereas the increased abundance of Duox1 in transfected wild-type Jurkat cells augmented interactions between ZAP-70 and Lck (Fig. 9F). Thus, these data suggest that Duox1-derived H2O2, through the oxidative inactivation of SHP-2, enhances the activation of ZAP-70 and the phosphorylation of its substrates.

Fig. 9
SHP-2–mediated regulation of the interaction among Lck, TCR, and ZAP-70. (A to C) Jurkat cells were transfected with empty vector, plasmid encoding SHP-2 (C/S), or plasmid encoding WT SHP-2 and stimulated by TCR cross-linking for 5 min as described ...


Intentional production of ROS, which function as requisite second messengers during signaling, occurs in a number of cell types in response to various stimuli. Decades ago, studies in T cells showed that exposure of cells to oxidizing agents such as pervanadate or H2O2 induced or enhanced the activation of proximal TCR signaling and T cell activation (46). Here, we demonstrated in T cells that the nonphagocytic NADPH oxidase Duox1 produced intracellular H2O2 upon TCR stimulation, which served in a positive feedback loop to enhance proximal TCR signaling. Duox1-derived generation of H2O2 in T cells was dependent on the presence of an intact TCR signaling pathway leading to the mobilization of intracellular Ca2+, including PLC-γ1 and IP3R1, because Duox1 is thought to be directly responsive to Ca2+ (42). The release of Ca2+ from intracellular stores was necessary for Duox1-dependent generation of ROS, whereas inhibition of the influx of extracellular Ca2+ did not lessen the production of ROS; however, an increase in the concentration of intracellular Ca2+ was not sufficient for the generation of ROS, which suggested that additional TCR signaling was required. Findings from another study (19), as well as from the current study, showed that proximal tyrosine kinases such as Lck and ZAP-70 control the generation of ROS. PKC was also implicated in our study as a necessary mediator of ROS generation. This finding is relevant because Duox1, Duox2, and the other Ca2+-dependent NADPH oxidase, Nox5, are all targets of serine kinases that modulate their activity (34, 43). Particularly in the case of Nox5, phosphorylation by PKC at a specific serine residue enhances the sensitivity of this enzyme to activation by lower concentrations of Ca2+. Here, the data indicate that Duox1, but not Duox2, was likely serine-phosphorylated upon TCR stimulation, and the lack of effects of Ca2+-mobilizing compounds, such as thapsigargin or ionomycin, by themselves on the generation of ROS suggests that this modification is required for the activation of Duox1 in T cells. Thus, TCR-induced activation of PKC may phosphorylate Duox1 and promote ROS generation in response to low concentrations of Ca2+.

The current study supports a hypothesis in which Duox1-derived H2O2 enhances TCR-proximal signaling events. Knockdown of Duox1 strongly suppressed TCR-stimulated tyrosine phosphorylation of selected targets and weakly inhibited the phosphorylation of TCRζ, but its major effect was on the phosphorylation of Tyr319 of ZAP-70. Phosphorylation of Tyr319 stabilizes the active state of ZAP-70 (35, 40) and provides an adaptor site for the SH2 domains of Lck or PLC-γ1(36, 41), so it could be a key regulatory point for TCR-proximal signaling. Cytotoxic T lymphocyte antigen 4 (CTLA-4) is an inhibitory co-receptor on T cells that dampens T cell responses. One proposed mechanism for its inhibition of TCR signaling is through the decreased phosphorylation of Tyr319 of ZAP-70, and the PTPs SHP-2 and SHP-1 seem to be responsible for this effect (44, 45). Although ZAP-70, especially Tyr319, is a substrate of Lck, our data suggest that Duox1-mediated regulation of Lck does not play a major role in the decreased phosphorylation of ZAP-70. Phosphorylation of the positive regulatory site in Lck (Tyr394) was not substantially affected by knockdown of Duox1. On the other hand, phosphorylation of the negative regulatory Tyr505 residue in Lck was substantially inhibited by knockdown of Duox1, suggesting that Duox1-dependent H2O2 limits the activity of Lck by augmenting the phosphorylation of Tyr505. These data suggest that a redox-sensitive target that leads to dephosphorylation of pTyr505 in Lck is affected by the activity of Duox1. The PTP CD45 was proposed to directly dephosphorylate pTyr505 (46), whereas SHP-2–mediated dephosphorylation of the adaptor protein PAG may inhibit the recruitment of Csk to PAG and the subsequent inhibitory phosphorylation of Lck at Tyr505 (9). Both PTPs are redox-sensitive and are inactivated by ROS (24, 47). Nevertheless, although some portion of Lck activity may be augmented by the knockdown of Duox1, the overall effects of knockdown of Duox1 on ZAP-70 seem to be dominant in its inhibitory effects on downstream signaling, including on direct ZAP-70 substrates, such as PLC-γ1 and LAT, Ca2+ flux, and ERK activation. Griffith et al. proposed that signaling through ZAP-70 to ERK activation was redox-sensitive on the basis of the observation that exogenous H2O2 did not induce the activation of ERK in the absence of ZAP-70 (48). Thus, our results further support a model in which ZAP-70 serves as a critical oxidant-regulated intermediary between TCR stimulation and downstream signaling.

As discussed earlier, one potential mechanism for the effects of Duox1 on Lck pTyr505 and ZAP-70 pTyr319 is through redox regulation of SHP-2. Our previous study demonstrated the ROS-mediated phosphorylation of SLP-76–associated proteins, such as Vav1 and ADAP, through TCR-induced oxidation of SHP-2 (24). The current data on the effects of knockdown of Duox1 on the phosphorylation of Vav1 and ADAP support the hypothesis that SHP-2 is a target of Duox1-generated H2O2 in T cells. These data are consistent with previous findings that suggest that SHP-2 associates with the adaptor protein Gab2, leading to its membrane localization near the TCR and proximal tyrosine kinases (11). SHP-1 is the other SH2 domain–containing PTP that is proposed to regulate TCR-proximal signaling, but our previous study showed that SHP-1 is not a good target of TCR-induced ROS (24), although it is oxidized upon the stimulation of T cells by antigen-presenting cells (49). Thus, the current data suggest that SHP-2 is recruited to the membrane, where Duox1-dependent H2O2 generation is prominent, and that it functions as a redox sensor that regulates proximal and distal TCR signaling events.

Our results further suggest that SHP-2, as a negative regulator, is recruited to a TCR–Lck–ZAP-70 complex through Gab2, and Duox1-mediated enhancement of TCR signaling results from H2O2-mediated inactivation of SHP-2. SHP-2 may also be recruited to these proteins in a Gab2-independent manner, but regardless, SHP-2–mediated dephosphorylation of pTyr319 of ZAP-70 inhibits the activation of ZAP-70 and its association with Lck, presumably through its SH2 domain. Thus, either siRNA-mediated knockdown of SHP-2 or expression of SHP-2 (C/S) enhanced the formation of the Lck–ZAP-70–TCRζ signaling complex, whereas knockdown of Duox1 inhibited the formation of the complex. Therefore, it seems that SHP-2 can terminate the amplification of an Lck–TCRζ–ZAP-70 feedback loop and that localized production of H2O2 by Duox1 inhibits SHP-2–mediated inhibitory effects on this complex.

A previous study of a B cell line suggested a positive feedback loop for Ca2+ and ROS in B cell receptor (BCR) signaling but through a very different mechanism (50). Data from that study suggested that the influx of extracellular Ca2+ was necessary for the production of ROS and the enhanced activation of the Src family tyrosine kinase Lyn. However, other studies suggested that ROS have a positive role in BCR signaling that is independent of Ca2+ (51) or that ROS from Nox2 inhibit the activation of B cells (52). In contrast, our current study on the role of H2O2 in TCR signaling suggests that both phosphorylation and Ca2+ released from the ER, not Ca2+ influx, contribute to the early TCR-dependent generation of H2O2 through the activation of Duox1. The early TCR-induced generation of H2O2 seems to inactivate the inhibitor SHP-2 through oxidation of an active-site cysteine, thereby enhancing and sustaining proximal TCR signaling.

Many questions remain, including those about the possible functions of Duox2 in T cells. Our data do not support a role for Duox2 in early TCR-stimulated generation of ROS, regulation of Ca2+, or activation of ERK. NOD2 has an important role in T cells (53), and a study has implicated Duox2 in NOD2-dependent signaling (54), so Duox2 may function more in innate responses by immune cells. Some data suggest that ROS produced by plasma membrane–associated NADPH oxidases leads to the oxidation of intracellular dyes; however, extracellular superoxide dismutase and catalase do not affect TCR-stimulated oxidation of DCFDA in human T blasts or Jurkat cells (fig. S1C) (21), suggesting an intracellular localization of Duox1 in T cells. The association of Duox1 with IP3R1 also supports this conclusion.

The regulation of Ca2+ flux by Duox1-derived H2O2 could occur at multiple steps in TCR signaling pathways. Our data suggest that enhanced TCR-proximal signaling through a H2O2-mediated positive feedback loop leads to augmented release of Ca2+ from the ER and the subsequent influx of extracellular Ca2+. Stimulation of the TCR results in co-capping of IP3R1 with the TCR at the sites of TCR engagement, which is where the cellular IP3-generating machinery composed of LAT and PLC-γ1 also accumulates (55). Our results support a model in which Duox1 constitutively associated with IP3R1 in the ER produces H2O2 locally near IP3R1-TCR clusters and regulates the tyrosine phosphorylation of proximal kinases. Duox1 may directly regulate the tyrosine phosphorylation of IP3R1, which is important for a sustained Ca2+ flux (55). In addition, the ER interacts with different compartments, such as the plasma membrane and mitochondria, through specialized junctions. Thus, Duox1-derived H2O2 might also regulate downstream Ca2+ mobilization at these sites. Alternatively, Ca2+ channels such as the IP3R1, the ryanodine receptor, and the Ca2+ release–activated Ca2+ (CRAC) channels composed of ORAI1 proteins are redox-sensitive (5658). The coimmunoprecipitation of Duox1 with IP3R1 suggests that Duox1 may mediate a redox-dependent effect on IP3R1-regulated Ca2+ flux.

Therefore, we propose a scheme of TCR-CD3 signal amplification through a positive feedback loop that involves Duox1-mediated production of H2O2 (Fig. 10). Upon TCR stimulation, SHP-2 associates with TCR-proximal signaling molecules through Gab2 and inhibits the activation of ZAP-70 through the dephosphorylation of pTyr319. Concomitantly, TCR-dependent signal transduction leads to the local production of H2O2 through Duox1 and oxidation of the active-site cysteine residue of SHP-2. Duox1-mediated inactivation of SHP-2 thereby diminishes its inhibitory activity and further enhances TCR signaling through the Lck–TCR–ZAP-70 pathway and T cell function.

Fig. 10
Proposed model for the Duox1-mediated regulation of TCR signaling. TCR stimulation leads, through the activation of several protein kinases, to the activation of PLC-γ1. The resulting hydrolysis of phosphatidylinositol-4,5-bisphosphate to diacylglycerol ...


Antibodies and reagents

A polyclonal antibody raised against the Arg618 to His1044 intracellular fragment of human Duox1 (originally called ThOx1) that recognizes both Duox1 and Duox2 was previously described (42). Antibodies against SHP-2, Grb2, ZAP-70, SHP-1, Vav, and Shc were from Transduction Labs/BD Biosciences. Polyclonal antibodies against Vav, Duox1, SHP-2, Grb2, and SHP-1 were purchased from Santa Cruz Biotechnology. Antibodies against Vav, SLP-76, Gads, Gab2, and phosphotyrosine (4G10); rabbit antibody against IP3R1; and horseradish peroxidase–conjugated rabbit antibody against sheep immunoglobulin G (IgG) were purchased from Upstate Biotechnology. Polyclonal antibody against Duox2 was obtained from Novus. Monoclonal antibody against pSer (clone 7F12) and the pSer detection set were obtained from Enzo Life Sciences. Antibody against pSer-containing PKC substrates (#2261) was from Cell Signaling Technology. All other chemicals were obtained from Sigma, and all cell culture supplies were from Life Technologies.

Cell culture

Human CD4+ T cells were purified from peripheral blood mononuclear cells by negative selection and activated for 2 days with plate-bound antibody against CD3 (OKT3) and soluble antibody against CD28. T cell blasts were washed and grown for 2 days in complete medium supplemented with recombinant human IL-2 (10 U/ml; Roche Molecular Biochemicals). Jurkat E6-1 cells (American Type Culture Collection) were maintained and propagated as previously described (24). HEK 293 Flp-In clones stably transfected with plasmid expressing hemagglutinin (HA)–tagged Duox1 or HA-tagged Duox2 were generated and maintained as described previously (59).

Plasmid constructs

Mammalian expression vectors that encode HA-tagged wild-type and catalytically inactive SHP-2 [SHP-2 (C/S)] have been previously described (24), as have those that encode wild-type PLC-γ1 and DNPLC-γ1[PLC-γ1 (Y783F)] (32). HA-tagged proteins were immunoprecipitated with the Profound HA-Tag IP/Co-IP kit (Pierce). Mammalian expression vectors encoding untagged and HA-tagged Duox1 have been previously described (59). N17Rac1 was expressed and the NFAT and AP-1 luciferase reporter constructs were used as previously described (26).

Transfection of cells with siRNA or shRNA

To knock down targets with specific siRNAs, we transfected Jurkat cells or activated human CD4+ T cells by electroporation at 250 mV and 950 μF with a GenePulser electroporator (Bio-Rad). The cells were then grown in RPMI 1640 medium with 10% human serum for 40 hours before their use in experiments. To knock down SHP-2, we used 5 × 106 cells per transfection with 0.7 nmol of control siRNA or a total of 0.75 nmol of SHP-2–specific siRNAs. Three different SHP-2–specific siRNAs (5′-GAACATCACGGGCAATTAATT-3′, 5′-GAACACTGGTGATTACTATTT-3′, and 5′-GAAGCACAGTACCGATTTATT-3′; obtained from Dharmacon) were pooled and used to knock down SHP-2. Initial studies compared three Duox1-specific siRNAs: siRNA D2, 5′-GGACTTCCATCGCCTCATT-3′ (D-008126-02, Dharmacon); siRNA J11, 5′-GAGATTCGGCGGAGGTTTG-3′ (J-008126-11, Dharmacon); and siRNA J12, 5′-GCACATCACACGGGCATCA-3′ (D-008126-12, Dharmacon). To knock down Duox1, we used 5 × 106 cells per transfection with 2.1 nmol of Duox1-specific siRNA (5′-GGACTTCCATCGCCTCATT-3′) or 2.1 nmol of control siRNA (ON-TARGETplus siCONTROL Non-Targeting siRNA, D-001810-01, Dharmacon). To knock down Duox1 with shRNA, we used the shRNA plasmid DNA containing the sequence CCGGCCTCATTTCCAAGGATGAGTTCTCGAGAACTCATCCTTGGAAATGAGGTTTTTG (TRCN0000045974, Sigma) and the NT shRNA plasmid (SHC002, Sigma). Jurkat cells transfected by electroporation with 1 μg of either NT shRNA or Duox1-specific shRNA were selected by culturing in the presence of puromycin (1.5 μg/ml) for 3 weeks. Clones were selected and expanded for an additional 2 months and used as stable clones.

Immunoprecipitations and Western blotting

Cell lysis, immunoprecipitations, and Western blotting analysis were performed essentially as previously described (24). Quantitation of bands was preformed by densitometry (Labworks version 4.6, UVP).

Detection of oxidized SHP-2

Jurkat cells were stimulated by cross-linking with antibody against CD3 (OKT3) as described previously (24). The oxidation of SHP-2 was measured as described (39) with some modifications. Cells were fractionated with cytoplasmic extraction buffer followed by membrane extraction buffer (Subcellular Protein Fractionation kit, Thermo Scientific) containing protease inhibitors (Roche) and 50 mM iodoacetamide. SHP-2 was immunoprecipitated from both cytoplasmic and membrane fractions with polyclonal antibodies against SHP-2 (C-18, Santa Cruz Biotechnology). Immunoprecipitated SHP-2 was treated serially with DTT and then pervanadate and analyzed with the affinity-purified oxPTP antibody (R&D Systems) as described previously (39).

Measurement of ROS in Jurkat cells

Kinetic assays of the TCR-stimulated generation of ROS in Jurkat cells measured the oxidation of DCFDA or dihydroethidium (DHE) within limited intervals after the stimulation of cells, essentially as described previously (25). Single endpoint measurements of early-phase generation of ROS in Jurkat cells and human CD4+ T cell blasts were determined by adding DCFDA to the cells before the stimulation of the TCR and measuring the stimulated oxidation of DCFDA over 10- or 15-min time courses, as described previously (26). The stimulated increase in dye oxidation was calculated as the percentage increase in mean channel fluorescence of cells stimulated with antibody against CD3 relative to that of unstimulated cells for each time point with the following equation: [(MCFstim − MCFunstim)/MCFunstim] × 100. The oxidation of DCFDA in transiently transfected cells was measured in cells that contained red fluorescent protein (RFP) encoded by a cotransfected plasmid, as previously described (26).

Measurement of Ca2+ flux by flow cytometry

Cells (1 × 106/ml) in phosphate-buffered saline were preloaded with Fluo-3 (2 μM) and Fura Red (2 μM) for 30 min in the dark at room temperature in the presence of pluronic and probenecid, as suggested by the supplier (Invitrogen). After washing, cells were resuspended at a density of 2 × 106 cells/ml in phenol red–free complete medium (with extracellular Ca2+) or in Hank's balanced salt solution containing 1% bovine serum albumin (without extracellular Ca2+) and warmed to 37°C. Baseline fluorescence was measured with a FACSCalibur flow cytometer for 30 s before the addition of a soluble stimulus: ionomycin (final concentration, 1 μg/ml) or an IgM against TCR (C305; 1:100 dilution of ascites). Fluorescence signals were collected for an additional 5 min and the data were analyzed with FlowJo software.

RT-PCR analysis

We purified mRNA from cell lysates with poly(dT) magnetic beads (Dynal Biotech) and reverse-transcribed the mRNA with SuperScript II (Invitrogen). PCR products were cloned into the pCRII-TOPO vector (Invitrogen) and sequenced with the M13 F primer. Primer sequences for the genes were as follows: Duox1, 5′-GCAGGACATCAACCCTGCACTCTC-3′ (forward) and 5′-CTGCCATCTACCACACGGATCTGC-3′ (reverse); Duox2, 5′-GCCCTCAACCTAAGCAGCTCACAAC-3′ (forward) and 5′-GAGCACAGTGAGATGCCTGTTCAG-3′ (reverse). The Duox1 PCR product was 532 base pairs (bp) and the Duox2 PCR product was 343 bp.

Measurement of cytokines

TCR-stimulated secretion of cytokines by purified CD4+ T cell blasts was measured by multiplex cytokine bead array kits (Bio-Rad), essentially as previously described (21). The lower limit of detection of all the cytokines was at 3.2 pg/ml. In this analysis, specimens with individual cytokine amounts that were below the lower limit of detection were arbitrarily assigned to be half of this value (1.6 pg/ml) for that cytokine.

Statistical analysis

Statistical significance was tested with the Wilcoxon signed rank test or the paired Student's t test with GraphPad Prism software. Data were considered significantly different when P < 0.05.

Supplementary Material

Supplementary Materials


We thank S. G. Rhee and A. Weiss for providing reagents for this study. We acknowledge J. Winkles and D. Leitenberg for critical review and comments on this manuscript. Funding: This work was supported by U.S. Public Health Service grant AI070823 (to M.S.W.); U.S. NIH Intramural Research Program, National Institute of Allergy and Infectious Diseases (to T.L.L. and J.K.); Fonds de la Recherche Scientific and Actions Concertées de la Communauté Française de Belgique (to X.d.D. and F.M.); and Fonds de la Recherche Scientifique Médicale (to F.M.).



Fig. S1. TCR-induced generation of ROS in Jurkat cells.

Fig. S2. Expression and function of Duox1 in human T cells.

Citation: J. Kwon, K. E. Shatynski, H. Chen, S. Morand, X. de Deken, F. Miot, T. L. Leto, M. S. Williams, The nonphagocytic NADPH oxidase Duox1 mediates a positive feedback loop during T cell receptor signaling. Sci. Signal. 3, ra59 (2010).

Competing interests: The authors declare that they have no competing interests.


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