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Targeting the (mChβGI)2 to the H19/Igf2 ICR.
The ICR was replaced by the (mChβGI)2. The CTCF sites were in the same orientation as the endogenous CTCF sites in the ICR. Novel restriction sites, such as StuI were generated at the sites of the binding site deletions. One control +/+ and four +/− ES cell clones are shown out of 24 that underwent conservative recombination. The loxP sites-flanked neo selection cassette was removed by Cre-mediated excision.