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By dual labeling a targeting moiety with both nuclear and optical probes, the ability for noninvasive imaging and intraoperative guidance may be possible. Herein, the ability to detect metastasis in an immunocompetent animal model of human epidermal growth factor receptor 2 (HER-2)-positive cancer metastases using positron emission tomography (PET) and near-infrared (NIR) fluorescence imaging is demonstrated. METHODS: (64Cu-DOTA)n-trastuzumab-(IRDye800)m was synthesized, characterized, and administered to female Balb/c mice subcutaneously inoculated with highly metastatic 4T1.2neu/R breast cancer cells. (64Cu-DOTA)n-trastuzumab-(IRDye800)m (150 µg, 150 µCi, m = 2, n = 2) was administered through the tail vein at weeks 2 and 6 after implantation, and PET/computed tomography and NIR fluorescence imaging were performed 24 hours later. Results were compared with the detection capabilities of F-18 fluorodeoxyglucose (18FDG-PET). RESULTS: Primary tumors were visualized with 18FDG and (64Cu-DOTA)n-trastuzumab-(IRDye800)m, but resulting metastases were identified only with the dual-labeled imaging agent. 64Cu-PET imaging detected lung metastases, whereas ex vivo NIR fluorescence showed uptake in regions of lung, skin, skeletal muscle, and lymph nodes, which corresponded with the presence of cancer cells as confirmed by histologic hematoxylin and eosin stains. In addition to detecting the agent in lymph nodes, the high signal-to-noise ratio from NIR fluorescence imaging enabled visualization of channels between the primary tumor and the axillary lymph nodes, suggesting a lymphatic route for trafficking cancer cells. Because antibody clearance occurs through the liver, we could not distinguish between nonspecific uptake and liver metastases. CONCLUSION: (64Cu-DOTA)n-trastuzumab-(IRDye800)m may be an effective diagnostic imaging agent for staging HER-2-positive breast cancer patients and intraoperative resection.
Molecular imaging with target-specific moieties conjugated to optical and nuclear reporters enables visualization of disease markers using noninvasive techniques, whereas optical reporters can additionally provide information for image-guided surgical procedures. Previously, we and others have synthesized and characterized dual-labeled peptide and antibody-based imaging agents in subcutaneous xenograft animal models [1–13]. In two of these studies [3,14], optical and nuclear imaging showed comparable tumor-to-muscle ratios (TMRs) after intravenous administration of a dual-labeled agent, whereas near-infrared (NIR) fluorescence optical imaging provided a significantly higher signal-to-noise ratio than gamma imaging. In this study, we designed a positron emission tomography (PET)/NIR imaging agent—(64Cu-DOTA)n-trastuzumab-(IRDye800)m—which consists of an antibody (trastuzumab) dual labeled with a beta emitter (Cu-64) for PET imaging and a NIR dye (IRDye 800CW) to enable fluorescence imaging of metastatic lesions overexpressing the human epidermal growth factor receptor 2 (HER-2). We used a syngeneic mouse model of an orthotopic mammary fat pad implantation, which exhibits metastases to organs similar to those affected in late-stage breast cancer. We used this model to test the ability of the dual-labeled imaging agent to detect metastases in sites distant from the primary tumor.
HER-2 is overexpressed because of gene amplification in approximately 20% to 30% of breast cancer patients, often leading to poor prognosis . HER-2 is predominantly conserved during metastases [16–19], making it a potential diagnostic biomarker. Trastuzumab (Herceptin; Genentech, Inc, San Francisco, CA) is a humanized anti-HER-2 antibody approved as a clinical therapeutic . Trastuzumab has been labeled by numerous investigators to generate imaging agents with applications including but not limited to nuclear, optical, and multimodal imaging (see Table W1). Yet to date, preclinical in vivo characterization of its use as an imaging agent has predominantly focused on subcutaneous tumor models using athymic mice.
Although the xenograft animal model is well established in cancer research to provide information regarding the interaction between the exogenously administered agent and the cancer cells in vivo, subcutaneous implantation of cancer cells neither realistically reproduces the microenvironment around the tumor region nor enables determination of whether the imaging agent can detect metastases in deep tissue organs. Xenograft models can approximate primary tumor growth in mice, but murine tumor models with normal immune function may be better suited to demonstrate detection of metastases. In this study, we use a syngeneic mouse model in which HER-2 expressing mammary cancer cells are introduced orthotopically into the mammary fat pad to mimic late-stage human breast cancer metastases. We have used this animal model to test the ability of the dual-labeled imaging agent to detect metastases in sites distant from the primary tumor and to compare to the clinical criterion standard of 18FDG-PET.
We purchased trastuzumab for research purposes at the hospital pharmacy and used it before the date of expiration. Trastuzumab was stored at 4°C. IRDye 800CW was purchased from LI-COR Biosciences (Lincoln, NB) and p-SCN-Bn-DOTA was purchased from Macrocyclics (Dallas, TX).
Trastuzumab was conjugated with p-SCN-Bn-DOTA using a previously reported protocol with slight modifications . In brief, trastuzumab was dissolved in HEPES buffer (0.1 M, pH 8.5) at a concentration of 10 mg/ml and mixed with 20-molar excess of p-SCN-Bn-DOTA (25 mg/ml) dissolved in ethanol. The reaction mixture was incubated at 4°C overnight. Purification of (DOTA)n-trastuzumab from excess chelator was achieved by using Zeba desalting columns (Pierce Biotechnology, Rockford, IL) with PBS in the mobile phase. Final concentration was measured based on UV absorbance at 280 nm using spectrophotometric analysis (model DU-800 instrument; Beckman Coulter, Brea, CA). (DOTA)n-trastuzumab (5 mg/ml) was then reacted with IRDye 800CW (30 µg/mg protein) dissolved in dimethyl sulfoxide (50 µl/mg dye) at 4°C for 2 to 3 hours. (DOTA)n-trastuzumab-(IRDye800)m was purified from free dye using Zeba desalting columns.
64Cu was obtained from Washington University Medical School (St Louis, MO) and supplied at high specific activity as 64CuCl2 in 0.1 M HCl. For radiolabeling, 64CuCl2 was diluted in ammonium acetate buffer (0.2 M, pH 5.5) at 50 mCi/ml and added to (DOTA)n-trastuzumab-(IRDye800)m. The reaction mixture was incubated at 50°C for 1 hour. (64Cu-DOTA)n-trastuzumab-(IRDye800)m was purified with PBS as the mobile phase using Zeba desalting columns, and radiolabeling yield was calculated using ITLC.
The average number of DOTA molecules per trastuzumab was estimated using a protocol previously described . In brief, 64CuCl2 was mixed with a defined amount of nonradioactive CuCl2 carrier (80-fold excess of (DOTA)n-trastuzumab-(IRDye800)m) and added to 50 µg of (DOTA)n-trastuzumab-(IRDye800)m in a total volume of 100 µl of 0.2 M sodium acetate buffer. The reaction mixture was incubated at 50°C for 1 hour. (64Cu-DOTA)n-trastuzumab-(IRDye800)m was purified using the Zeba desalting column, and radiolabeling yield was calculated. The number of DOTA molecules per trastuzumab (n) was calculated as follows:
The IRDye800 to trastuzumab ratio (m) is estimated using the absorbance measurements of the dye and protein at 778 and 280 nm, respectively, as described by the labeling protocol from LI-COR Biosciences. A correction factor of 3% was subtracted from the measurement at 280 nm because of dye contribution.
Cell lines used in this study include human SKBr3 and MDA-MB-231 and murine 4T1.2, 4T1.2neu, 4T1.2R, and 4T1.2neu/R breast cancer cells. SKBr3 cells express high levels of HER-2, whereas MDA-MB-231 cells express negligible amounts of HER-2. SKBr3 and MDA-MB-231 cell lines were obtained from American Type Culture Collection (Manassas, VA). 4T1.2 is a subline of breast cancer cells derived from spontaneous carcinoma in a Balb/cfC3H mouse . Because HER-2 is highly conserved across species, we used 4T1.2neu cells, which were established by transducing the rat homolog of HER-2, neu, into 4T1.2 breast cancer cells with a retroviral vector, pFB-Neu-Neo . Both 4T1.2 and 4T1.2neu were generously provided by Zhaoyang You (University of Pittsburg School of Medicine, Pittsburgh, PA). We transfected both these cell lines with p-DsRedExpress-N1 (Clontech, Mountain View, CA) to generate 4T1.2R and 4T1.2neu/R which were selected by flow cytometry using a Becton-Dickinson FACS Aria flow cytometer (BD Biosciences, San Jose, CA). The DsRed-expressing cells were cultured in Dulbecco's minimal essential medium F-12 with 10% fetal bovine serum, 1% antibiotic-antimycotic solution, and 1 mg/ml of G418 in a humidified incubator maintained at 37°C with 5% CO2.
Cells were grown on coverslips in a 24-well dish. When cells reached 90% confluence, cells were washed and blocked with Odyssey Blocking Buffer (LI-COR Biosciences) for 1 hour. Cells were then incubated with primary mouse anti-HER-2 antibodies or (Cu-DOTA)n-trastuzumab-(IRDye800)m diluted in Odyssey Blocking Buffer for 1 hour at 4°C. Cells were washed and stained with secondary antibodies, goat antimouse AlexaFluor 488 or mouse antihuman fluorescein isothiocyanate, respectively, for 30 minutes at 4°C. After this incubation period, cells were washed again, and the cover slips were placed on a glass slide with mounting medium containing 4′-6-Diamidino-2-phenylindole nuclear stain (Vectashield; Vector Laboratories, Burlingame, CA).
All images were acquired using a Leica DFC350FX microscope (Leica Microsystems, Inc, Bannockburn, IL) connected to a computer. Images were processed using Leica Application Suite software or Image J (National Institutes of Health, Bethesda, MD).
4T1.2 and 4T1.2neu cells were seeded in a 96-well plate and grown overnight. Cells were fixed and blocked using Odyssey Blocking Buffer (LI-COR Biosciences) for 1 hour at room temperature. Mouse antirat HER-2/neu primary antibody (Calbiochem, La Jolla, CA) was diluted in blocking buffer at a concentration of 5 µg/ml and added to the cells to incubate for 2.5 hours at room temperature. Cells were washed with Tris-buffered saline and Tween 20 and IRDye 800CW-labeled goat antimouse IgG secondary antibody (LI-COR Biosciences) was added to cells at a dilution of 1:1000. For normalization of cell number, DRAQ5 (LI-COR Biosciences) diluted at 1:5000 was also added to the cells and allowed to incubate for 1 hour. After repeated washing, the wells were allowed to dry and fluorescence imaging was performed on the Odyssey infrared imaging system (LI-COR Biosciences) using the 800-nm channel to measure HER-2 expression and the 700-nm channel to normalize for cell count using fluorescence from DRAQ5.
Female Balb/c mice (8 weeks old; Charles River, Wilmington, MA) were housed and maintained in a specific pathogen-free colony at the Institute of Molecular Medicine, UT-Health Science Center, and were fed standard chow diet. The facility is accredited by the American Association for Laboratory Animal Care, and all experiments were performed in accordance with the guidelines of UT-Health Science Center Institutional Animal Care and Use Committee. Animals were subcutaneously injected with 4T1.2neu/R (1-2 x 106 in 20 µl) into the fourth mammary fat pad. Initial tumor growth was monitored with DsRed fluorescence imaging. Before imaging, mice were shaved, and residual fur was removed using a depilatory agent. 18FDG (200–250 µCi) or (64Cu-DOTA)n-trastuzumab-(IRDye800)m (150 µg, 150 µCi, n = 2, m = 2) were injected intravenously at week 2 in 4T1.2neu/R (N = 5) or at week 6 in 4T1.2R (N = 6), 4T1.2neu/R (N = 6) and non-tumor-bearing (N = 3) mice, for subsequent microPET/computed tomography (CT) and NIR fluorescence imaging. 18FDG was injected first, and PET/CT imaging was performed. (64Cu-DOTA)n-trastuzumab-(IRDye800)m- was injected after 10 half-lives of F-18 radioactive decay, followed by NIR fluorescence imaging and PET/CT approximately 24 hours later. The imaging time point was determined based on the radioactive half-life of 64Cu (t1/2 = 12.7 hours), although no significant increase in tumor uptake has been shown with NIR fluorescence imaging at time points greater than 24 hours . After in vivo imaging techniques, mice were euthanized, and select organs were harvested for ex vivo NIR fluorescence imaging. For further histopathologic analysis, tissue samples were placed in 10% formalin overnight and sent to the Center for Comparative Medicine Pathology Core (Baylor College of Medicine, TX) before undergoing routine histologic processing, sectioning, and hematoxylin and eosin (H&E) staining.
NIR and DsRed fluorescence images were acquired using custom-built fluorescence imaging systems . Briefly, a field of view was illuminated with 785 or 568 nm of light from a laser diode (500 mW; Intense Ltd, North Brunswick, NJ) or air-cooled argon-krypton laser (Model 643R-AP-A01, 200 mW; Melles Griot, Carlsbad, CA), respectively, outfitted with a convex lens and diffuser to create a uniform excitation field. The fluorescence was collected through holographic (model HNPF-785.0-2.0 for NIR and model 568.2–6 for DsRed; Kaiser Optical Systems, Inc, Ann Arbor, MI) and interference (model 830.0-2.0 for NIR, Image Quality [Andover Corp, Salem, NH] and model HQ610/60M for DsRed [Chroma Technology Corp, Bellows Falls, VT]) filters placed before a Nikon camera lens (Nikkor 28 mm; Nikon, Tokyo, Japan). The images were finally captured by an electron-multiplying charge-coupled device camera (PhotonMax 512; Princeton Instruments, Princeton, NJ) with 200 to 400 milliseconds of integration time. For acquisition of white-light images, the optical filters were removed, and a low-power lamp illuminated the subject. Image acquisition was accomplished by V++ software (Aukland, New Zealand).
Whole-body fluorescence images were acquired 24 hours after intravenous administration of (64Cu-DOTA)n-trastuzumab-(IRDye800)m (150 µg, 150 µCi). On sacrifice, ex vivo fluorescence imaging of harvested organs was also performed.
In vivo small animal PET/CT imaging studies were performed using an INVEON dedicated PET docked with an INVEON multimodality CT (Siemens Medical Solutions, Knoxville, TN). The CT imaging parameters were an x-ray voltage of 80 kV with an anode current of 500 µA and an exposure time of 260 milliseconds of each of the 120 rotation steps over the total rotation of 220° at low system magnification. After CT imaging, PET emission scans were performed on separate days with acquisition times of 5 (18F) and 10 minutes (64Cu). PET and CT images were reconstructed using two-dimensional filtered back-projection and a Feldkamp cone-beam algorithm with a ramp filter cutoff at the Nyquist frequency, respectively. PET and CT image fusion and image analysis were performed using ASIPro, Inveon Research Workplace (Siemens Preclinical Solutions) and AMIRA (version 3.1; Konrad-Zuse-Zentrum fur Informationstechnik, Berlin, Germany).
All data were analyzed using one-way analysis of variance, the significance level was set at 0.05, and all quantitative data are represented as mean ± SD. To determine TMR or lung-to-muscle ratio (LMR) from NIR fluorescence images, a region-of-interest was drawn around the tumor, lung, or muscle region, and the total fluorescence signal intensity associated with the region was computed. TMR or LMR from NIR fluorescence images was calculated as follows:
To calculate TMR from PET images, tumor standardized uptake value (SUV) and the contralateral muscle SUV were computed using the Inveon Research Workplace (Siemens Preclinical Solutions). TMR calculations were performed as follows:
Trastuzumab was conjugated to p-SCN-Bn-DOTA and IRDye 800 CW, resulting in approximately 2.4 ± 0.2 DOTA molecules per antibody (n). The IRDye800 to trastuzumab ratio was 2.2 ± 0.3 (m). The yield of (DOTA)n-trastuzumab-(IRDye800)m after purification was greater than 85%. Radiolabeling yield with Cu-64 was greater than 90%. Immunoreactivity as determined by the cell-binding assay described by Lindmo et al.  was determined to be 76.4% ± 3.6%.
Figure 1 illustrates binding of (Cu-DOTA)n-trastuzumab-(IRDye800)m in SKBr3 (HER-2-overexpressing) and MDA-MB-231 (low expressors of HER-2) cells as visualized through fluorescence microscopy. The imaging agent binds with high affinity to SKBr3 cells, whereas there is negligible binding in MDA-MB-231 cells. Also, the binding seems to be extracellular, which is consistent with the location of the transmembrane HER-2 receptors. The dual-labeled imaging agent bound to greater than 90% of SKBr3 cells, as determined by NIR FACS.
Figure 2A represents an in-cell Western blot to quantify the difference in HER-2 levels between 4T1.2 and 4T1.2neu cells. The top panel in Figure 2A shows the fluorescence signal from HER-2/neu staining in cells, the middle panel shows the DRAQ5 nuclear staining for normalization with cell number, whereas the bottom panel shows the combined fluorescence signal. The quantitative results are depicted graphically in Figure 2B. Figure 2C compares the binding of (Cu-DOTA)n-trastuzumab-(IRDye800)m in DsRed-transfected 4T1.2R and 4T1.2neu/R cells. Both cell lines express DsRed as illustrated, but 4T1.2neu/R has a significantly higher fluorescence because of binding of the dual-labeled imaging agent compared with 4T1.2R.
Because DsRed expression was lost with tumor progression, DsRed fluorescence imaging was conducted to assess inoculation and initial growth. DsRed fluorescence imaging of a shaved Balb/c mouse with a subcutaneous 4T1.2neu/R tumor confirms the location of the tumor (Figure 3A). The corresponding NIR fluorescence image acquired 24 hours after (64Cu-DOTA)n-trastuzumab-(IRDye800)m administration is shown in Figure 3B. PET signal fused with CT after (64Cu-DOTA)n-trastuzumab-(IRDye800)m and 18FDG administration revealed significant uptake within the tumor compared with the muscle region. Representative coronal sections are represented in Figure 3, C and D, with crosshairs indicating tumor location. In addition to tumor uptake, the dual-labeled imaging agent accumulates within the liver, as seen in both PET and NIR fluorescence images. The nonspecific liver uptake may be attributed to the interaction of the Fc portion of the antibody with hepatocytes and is consistent with the site of antibody degradation and clearance.
Tumor uptake after 18FDG and (64Cu-DOTA)n-trastuzumab-(IRDye800)m administration shows comparable results as observed by PET imaging, but differences were observed in the in vivo distribution between the two imaging agents. In vivo TMRs between 18FDG-PET, 64Cu-PET, and NIR imaging ranged from 2.09 ± 0.37, 2.21 ± 0.40, and 2.81 ± 0.70, respectively (Figure 3E). No statistically significant differences (P > .1) between the TMR were observed between the modalities, indicating that the diagnostic capabilities of the molecularly specific dual-labeled imaging agent—(64Cu-DOTA)n-trastuzumab-(IRDye800)m—are similar to those of clinically approved but nonspecific 18FDG for detecting primary tumors.
Metastases in the 4T1.2 animal model are known to occur by 5 to 6 weeks . To assess the imaging capabilities of (64Cu-DOTA)n-trastuzumab-(IRDye800)m in detecting HER-2-overexpressing metastatic lesions arising from the primary tumor, we acquired PET/CT and NIR fluorescence images after administration of the dual-labeled imaging agent in 4T1.2neu/R tumor-bearing Balb/c mice and compared the results with 18FDG. We also compared uptake between nontumor control, 4T1.2R, and 4T1.2neu/R tumor-bearing mice injected with (64Cu-DOTA)n-trastuzumab-(IRDye800)m and 18FDG. Figure 4 shows NIR fluorescence, 64Cu-PET, and 18FDG-PET images from a representative mouse from each group. All mice showed nonspecific uptake of the dual-labeled imaging agent in the liver. The primary tumor region of the 4T1.2R tumor-bearing mice showed some uptake of the imaging agent, but there was significantly higher binding within the 4T1.2neu/R tumors as confirmed ex vivo. Nevertheless, because 18FDG is molecularly nonspecific, it did not differentiate between the two tumor types, and we were able to detect both 4T1.2R and 4T1.2neu/R tumors. Although we were able to visualize the primary tumor with all modalities, 18FDG-PET was not effective in detecting metastases. In contrast, we were able to detect multiple sites of 4T1.2neu/R metastases with the molecularly specific dual-labeled (64Cu-DOTA)n-trastuzumab-(IRDye800)m. Owing to intrinsic differences between PET and NIR imaging capabilities, the metastatic lesions identified through both modalities complemented each other. Lung metastases were clearly visualized noninvasively in vivo with 64Cu-PET (Figure 4, B and D) in 4T1.2neu/R tumor-bearing mice, whereas limitations associated with scatter in deep tissue prevented detection with planar in-vivo NIR fluorescence imaging. Figure 4, D and E, I and J, and N and O, shows PET/CT sections acquired after (64Cu-DOTA)n-trastuzumab-(IRDye800)m and 18FDG administration in representative 4T1.2neu/R, 4T1.2/R, and non-tumor-bearing mice, respectively. The crosshairs indicate lung location. 18FDG-PET from both the tumor- and non-tumor-bearing mouse shows no accumulation within the lung. In contrast, 64Cu-PET shows significant accumulation of the dual-labeled imaging agent within the lung region of a 4T1.2neu/R tumor-bearing mouse compared with the 4T1.2R and nontumor control.
The high spatial resolution achieved using NIR fluorescence imaging enabled us to detect metastatic lesions (~2 mm diameter) close to the skin (Figure 5, A and B), whereas PET imaging could not identify them. Optical imaging enabled us to visualize trafficking of the imaging agent from the primary tumor to proximal and distant lymph nodes when we removed the skin to reveal the mammary pad (Figure 5, C through E). This correlates with previous reports of lymphatic involvement in tumor growth and metastatic process of this animal model . When we performed whole skin dissection of the tumor-bearing mouse, in addition to visualizing the primary tumor, we observed accumulation of the imaging agent in lymph nodes and sections of the mammary pad. A representative photograph and NIR fluorescence image of a whole skin dissection is represented in Figure 5, F and G, respectively. In addition to the primary tumor, the tumor-draining lymph node (inguinal) is fluorescent and confirmed to be cancer-positive from H&E histologic staining. The skin metastatic lesions are also indicated.
We identified a total of 15 fluorescent lymph nodes from six tumor-bearing mice used in this study and performed further investigation using histologic staining. Only seven nodes were infiltrated by cancer cells. However, extensive inflammation and presence of granulopoieisis was observed in all the lymph nodes in addition to spleen and liver, consistent with previous reports [26–29]. Although in vivo whole-body NIR fluorescence imaging of (64Cu-DOTA)n-trastuzumab-(IRDye800)m did not show accumulation within the lung, we were able to confirm the presence of the imaging agent ex vivo on harvesting the organs after sacrifice (Figure 6A). Representative histologic H&E staining of the 4T1.2neu/R primary tumor and lung, lymph nodes, muscle, and skin metastases are shown in Figure 6, B to F.
We removed the skin of mice to reveal the mammary pad and compared whole skin dissections (Figure 7, A–F) within the three groups of mice to assess the specificity of 64Cu-DOTA)n-trastuzumab-(IRDye800)m binding in lymph nodes. The nontumor control mice and the mice inoculated with 4T1.2/R did not show accumulation of the imaging agent within the mammary pad or lymph nodes. In contrast, mice with 4T1.2neu/R tumors showed an increased background fluorescence signal within the entire skin in addition to sections of the mammary pad. Figure 7F shows another representative image where trafficking of the imaging agent from the primary tumor and its associated lymph node to the axillary region can be visualized.
We hypothesize that the NIR fluorescence signal from within lymph nodes and the mammary pad may be due to the interaction of the antibody-based imaging agent with elevated levels of HER-2/neu antigen expression as a result of the 4T1.2neu/R tumor.
The ex vivo NIR fluorescence LMR in 4T1.2neu/R tumor-bearing mice (2.85 ± 0.67) was found to be statistically higher in comparison with non-tumor-bearing mice (1.23 ± 0.15) with a P < .05 (Figure 7H). The large error bar found in the LMR in tumor-bearing mice can be due to the variability in the extent of lung metastases within each mouse.
Ex vivo NIR fluorescence TMR estimated from 4T1.2neu/R tumors (4.03 ± 0.57) was also found to be statistically higher than 4T1.2R tumors (1.83 ± 0.33) (P < .05) as shown in Figure 7G. LMR of 4T1.2neu/R tumor-bearing mice (2.85 ± 0.67) was found to be statistically higher in comparison with 4T1.2/R (1.45 ± 0.19) and non-tumor-bearing mice (1.23 ± 0.15) with a P < .05 (Figure 7H).
According to the National Cancer Institute, approximately 191,000 new cases of breast cancer and 41,000 deaths were reported in 2009. Patient mortality and morbidity is often due to late-stage complications that arise from progression of the disease to distant metastatic sites. In breast cancer, the most common sites of metastases include lung, liver, bone, and brain. Metastasis is a complex and multistep process where the tumor cells have to detach from the primary tumor, enter the lymphatic or blood circulation, reach a distant site, attach, and proliferate. Molecular imaging techniques have immense potential in enhancing early stage diagnostics, monitoring treatment efficacy, and affecting drug discovery.
The 4T1 animal model was originally isolated by Fred Miller et al.  at the Karmanos Cancer Institute. By 6 weeks, 4T1 cells and subclones have been shown tometastasize to various organs including lungs, liver, and bone [23,26,31,32]. Although a number of investigators have used cancer cell lines expressing reporters to visualize cancer migration during metastases [33–39], very few studies have been performed where an exogenous diagnostic agent is administered to detect metastases within an animal model. In a recent report, Cao et al.  synthesized 64Cu-DOTA-IL-18bp-Fc to assess the tumor-targeting efficiency and pharmacokinetics in interleukin-18 binding protein-sensitive cells using PET imaging in a lung metastases model. In the present study, we have demonstrated the targeting capabilities of (64Cu-DOTA)n-trastuzumab-(IRDye800)m in vitro using fluorescence microscopy and with an in vivo cancer model to assess uptake in primary tumors and subsequent metastases after intravenous administration of the imaging agent. Although there have been several studies using trastuzumab labeled for imaging with nuclear, magnetic resonance imaging, and optical techniques (see Table W1), this study is one of the first reports to use it in a dual-labeled diagnostic imaging agent to identify distant organ metastases. Because molecular-specific optical and nuclear probes have unique sets of advantages, combining modalities offers a wider range of opportunities for early detection of disease. Deep tissue penetration by radioactive isotopes enabled detection of lung metastases, whereas NIR fluorescence imaging provided information regarding lymphatic involvement. In addition to nonspecific radiocolloids used in the clinic, molecular imaging approaches such as these can potentially enhance the accuracy of nodal staging, although human trials will have to be conducted to confirm benefit.
Dual-labeled (64Cu-DOTA)n-trastuzumab-(IRDye800)m was tested for molecular specificity in HER-2-overexpressing breast cancer cells as well as in a relevant metastatic animal model to assess and confirm targeting in vitro and in vivo. Furthermore, we have compared the detection capabilities of the imaging agent with 18FDG and have showed that (64Cu-DOTA)n-trastuzumab-(IRDye800)m has greater sensitivity in detection of metastases overexpressing HER-2.
1This work is supported in parts by R01 CA112679-15 (E.M.S.-M.), U54 136404-01 (E.M.S.-M.), the Texas STAR Award program, and Department of Defense predoctoral fellowship award BC073377 (L.S.). Cu-64 provided by Washington University, St. Louis, MO, was partially funded by National Cancer Institute grant R24 CA86307.