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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
J Neurosci. Author manuscript; available in PMC 2010 December 9.
Published in final edited form as:
PMCID: PMC2935280
NIHMSID: NIHMS212070

Rab11 Supports Amphetamine-Stimulated Norepinephrine Transporter Trafficking

Abstract

The norepinephrine transporter (NET) is a presynaptic plasma membrane protein that mediates reuptake of synaptically released norepinephrine (NE). NET is also a major target for medications used for the treatment of depression, attention-deficit hyperactivity disorder, narcolepsy, and obesity. NET is regulated by numerous mechanisms, including catalytic activation and membrane trafficking. Amphetamine (AMPH), a psychostimulant and NET substrate, has also been shown to induce NET trafficking. However, neither the molecular basis nor the nature of the relevant membrane compartments of AMPH-modulated NET trafficking has been defined. Indeed, direct visualization of drug-modulated NET trafficking in neurons has yet to be demonstrated. In this study, we utilized a recently developed NET antibody and the presence of large presynaptic boutons in sympathetic neurons to examine basal and AMPH-modulated NET trafficking. Specifically, we establish a role for Rab11 in AMPH-induced NET trafficking. First, we found that in cortical slices, AMPH induces a reduction in surface NET. Next, we observed AMPH-induced accumulation and colocalization of NET with Rab11a and Rab4 in presynaptic boutons of cultured neurons. Using tagged proteins, we demonstrated that NET and a truncated Rab11 effector (FIP2ΔC2) do not redistribute in synchrony whereas NET and wild type Rab11a do. Analysis of various Rab11a/b mutants further demonstrates that Rab11 regulates NET trafficking. Expression of the truncated Rab11a effector (FIP2ΔC2) attenuates endogenous Rab11 function and prevented AMPH-induced NET internalization as does GDP-locked Rab4 S22N. Our data demonstrate that AMPH leads to an increase of NET in endosomes of single boutons and varicosities in a Rab11-dependent manner.

Keywords: Norepinephrine, transporter, amphetamine, Rab11, endocytosis, clathrin

Introduction

The norepinephrine transporter (NET) is a presynaptic plasma membrane protein with a critical role in the reuptake of released norepinephrine (NE) (Iversen, 1971; Pacholczyk et al., 1991; Bonisch and Bruss, 2006). The importance of NET for NE-mediated physiology and behavior is demonstrated both by the phenotypes of mice lacking NET (Bohn et al., 2000; Xu et al., 2000; Haller et al., 2002; Keller et al., 2004; Kaminski et al., 2005; Keller and Robertson, 2006) and by the consequences of polymorphisms in the human NET gene (Klimek et al., 1997; Rumantir et al., 2000; Shannon et al., 2000; Hahn and Blakely, 2002; Hahn et al., 2003; Hahn et al., 2005; Esler et al., 2006; Kim et al., 2006; Haenisch et al., 2008; Hahn et al., 2008). Several of these polymorphisms result in an altered level of surface NET and reduced NE transport (Hahn et al., 2003; Hahn et al., 2005; Kim et al., 2006; Haenisch et al., 2008).

Studies over the past two decades have revealed that NET is highly regulated (Bonisch and Bruss, 2006; Mandela and Ordway, 2006). NET regulatory pathways can impact both transporter turnover rate and the level of surface NET (Apparsundaram et al., 1998b; Apparsundaram et al., 1998a; Uchida et al., 1998; Bauman et al., 2000; Torres et al., 2001; Sung et al., 2003; Sung et al., 2005; Wersinger et al., 2006) providing mechanisms to fine-tune NE homeostasis (Bonisch and Bruss, 2006). AMPH, an abused substance, also alters NE signaling, in part through actions on NET. This leads to NET mediated NE efflux and, possibly, NET trafficking. Importantly, AMPH effects on NET may underlie vasculitis, neuropsychiatric abnormalities, and cardiomyopathy observed in abuse of the psychostimulant (Furst et al., 1990; Varner et al., 2002; Pozzi et al., 2008). Therefore, an understanding of how AMPH regulates neuronal NET including elucidation of the mechanisms and membrane compartments underlying AMPH-modulated NET trafficking may identify useful therapeutic strategies to combat the occurrence and sequelae of AMPH addiction.

A substantial portion of NET resides in intracellular vesicles of unknown nature (Leitner et al., 1999; Schroeter et al., 2000; Miner et al., 2003; Miner et al., 2006). Despite a previous hypothesis (Kippenberger et al., 1999; Schroeter et al., 2000; Savchenko et al., 2003), neuronal NET does not appear to sort to dense core vesicles (Leitner et al., 1999; Matthies et al., 2009) nor to several other presynaptic membrane compartments (Matthies et al., 2009). Thus, endocytic compartments are a reasonable candidate. Potential endosomal pathways include early endosomes (“rapid” rab5-positive pathway), or recycling endosomes (“slow” rab11-positive pathway), and NET could be returned to the plasma membrane from either the “rapid”, or “slow” pathway (Mellman, 1996; Robinson et al., 1996; Mukherjee et al., 1997; Novick and Zerial, 1997; Somsel Rodman and Wandinger-Ness, 2000; Zerial and McBride, 2001; Maxfield and McGraw, 2004).

Based on a preliminary observation of AMPH-induced internalization of heterologously expressed NET (Dipace et al., 2007), we hypothesized that AMPH could trigger NET internalization in neurons. In this report, we confirm this idea and demonstrate that this effect is due to mobilization of plasma membrane NET, in a Rab11-and Rab4-dependent manner.

Materials and Methods

Brain slice preparation and biotinylation

Brain slices were prepared from 6- to 12-week-old rats that were anesthetized with isoflurane and rapidly decapitated. Following, brain removal the brain was chilled in oxygenated ≈4°C modified ACSF (sucrose 210mM; NaCl 20mM; KCl 2.5mM; MgCl2 1mM; NaH2PO4•H2O 1.2mM). The brain is then placed in a pre-chilled rodent brain matrix (rat, coronal slices, ASI Instruments, Warren, MI, item # RBM-4000C). Cortical slices were then collected in oxygenated artificial cerebral spinal fluid (ACSF) (NaCl 125mM, KCl 2.5mM, NaH2PO4•H2O 1.2mM, MgCl2 1mM, CaCl2•2H2O 2mM). The slices were then transferred to a 28°C bath, allowed to equilibrate for 1 hour, after which amphetamine (10 μM final) was added for 30 minutes. The drug reaction was terminated by removal from the bath and washing twice with oxygenated ACSF, and incubated with 4°C ACSF solution containing 1mg/mL of EZ-Link Sulfo-NHS-SS-Biotin (Pierce Chemical; Rockford, IL) for 45 minutes (4°C). After biotin incubation, the slices were rinsed twice quickly and for two 10 minutes washes in oxygenated ACSF (4°C). The reaction was quenched by washing twice for 20 minute each with oxygenated ACSF containing glycine (4°C). Following quenching, slices were frozen on dry ice and the cortex dissected and frozen at −80°C until used. Slices were lysed in 200 μl 1% Triton buffer (25mM Hepes, 150mM NaCl, 2mM Sodium orthovanadate, 2mM NaF, plus a cocktail of protease inhibitors) and centrifuged at 17,000g for 30 minutes at 4°C. After isolation of supernatant, 400 μl of 0.1% Triton pulldown buffer (25mM HEPES, 150mM NaCl, 2mM Sodium orthovanadate, 2mM NaF, plus a cocktail of protease inhibitors) was added. The protein concentration was determined using Bio-Rad’s protein concentration kit. Equivalent protein amounts of biotinylated proteins (supernatant) were then isolated using 50 μl of ImmunoPure immobilized streptavidin beads (Pierce) overnight at 4°C with agitation. Beads were washed three times with 0.1% Triton pulldown buffer and biotinylated proteins were then eluted in 50 μL of 2X SDS-PAGE sample loading buffer at 95°C and then cooled to room temperature. Total slice lysates and the biotinylated (slice cell surface) fractions underwent immunodetection for NET (MabTechnoligies Inc. Stone Mountain, GA) (Matthies et al., 2009) tyrosine hydroxylase, and Na/K ATPase.

Cell culture and transfection

Mouse superior cervical ganglion neurons (SCGNs) were cultured as previously described (Savchenko et al., 2003; Matthies et al., 2009). Briefly, superior cervical ganglions were dissected from 1-3 day old C57BL/6J mice and dissociated for 30 minutes in 3 mg/mL collagenase/0.5 mg/mL trypsin, followed by 10% FBS in DMEM. Cells were plated and incubated with 3% fetal bovine serum in UltraCulture medium containing NGF for 2 hours at 37° C to allow fibroblasts to adhere. Supernatant medium (containing SCG cells) was centrifuged, resuspended in medium supplemented with FBS, and cultured on poly-D-lysine- and collagen-coated glass-bottom plates for 14 days before experiments (treating with 1 μM 5-fluoro-5-deoxyuridine after 24 hours).

CAD cells are derived from transformed noradrenergic precursors that synthesize catecholamines but do not endogenously express NET (Qi et al., 1997). Human NET (hNET) was cloned into pcDNA3, stably transfected into CAD cells, and selected and maintained with 100 μg/mL G418, creating ‘NET cells’ (Sung et al., 2003). CAD or NET cells were maintained in serum free media to induce neuronal differentiation (Qi et al., 1997).

mCherry-Rab11-FIP2ΔC2, RFP-Rab11a, are based on previous constructs (Hales et al., 2002; Lapierre et al., 2003) but are fusion proteins of mCherry or mRFP. mRFP and mCherry are gifts of Roger Tsien. mCherry-Rab11-FIP2ΔC2 was generated by Jennifer Schafer and RFP-Rab11a by Joseph Roland. GFP-NET was constructed by inserting NET into pEGFP-C2 from Clontech. Human Rab11b cDNA was isolated and cloned into mCherry vectors by standard methods. Rab11 mutants were also generated by standard methods. Transient transfections were performed using Fugene 6 reagent (Roche).

Antibodies and other reagents

Monoclonal anti-mNET (NET-05 MAb Technologies, Stone Mountain, GA (Matthies et al., 2009), monoclonal anti-hNET 17-1 (MAb Technologies, Atlanta, GA), monoclonal anti- Na+/K+ ATPase α subunit (Developmental Studies Hybridoma Bank), polyclonal Tyrosine Hydoxylase (Cell Signalling), polyclonal anti-Rab11a (Zymed), and polyclonal anti-Rab11a (Lapierre et al., 2001) were used at dilutions of 1:300, 1:1000, 1:100, 1:1000, 1:500, and 1:250-1:1000, respectively, for immunoblotting, with detection by enhanced chemiluminescence reaction. Monoclonal anti-mNET (NET-05 MAb Technologies, Stone Mountain, GA (Matthies et al., 2009), FIP1 (Hales et al., 2001), pp75/FIP5 (Hales et al., 2001), and polyclonal anti-Rab11a (as described above) were used at dilutions of 1:500, 1:110, 1:200, and 1:1000 for immunocytochemistry. Fluorescent secondary antibodies included highly cross-absorbed goat anti-mouse or anti-rabbit IgG antibodies (Molecular Probes). AMPH was obtained from Sigma (St. Louis, MO).

Cell surface protein biotinylation

Biotinylation experiments were performed on intact cells as described previously (Sung et al., 2003; Garcia et al., 2005; Dipace et al., 2007). Briefly, CAD NET cells were plated at a density of 1 × 106 per well in a six-well poly-(D-lysine) coated plate. Cells were serum starved for 1 hour prior to treatment, and washed with cold PBS containing Ca2+/Mg2+. Then, cells were incubated with 1.0 mg/mL sulfosuccinimidyl-2-(biotinamido)ethyl-1,3-dithiopropionate [NHS-SS-biotin] (Thermo Scientific, Rockford, IL) for 30 minutes, washed, quenched with 100 mM glycine, and extracted in lysis buffer (PBS Ca2+/Mg2+, 1% Triton 100-X, and 0.5 mM PMSF at 4C). Lysates were centrifuged, total fractions reserved, and supernatants incubated with immobilized streptavidin beads (ThermoScientific) for 1 hour at room temperature. Beads were washed three times in lysis buffer, and bound proteins eluted with 2X sample buffer containing 2-mercaptoethanol. Proteins were separated by SDS-PAGE and immunoblotted. For estimation of relative amounts of proteins, the exposed films of the immunoblots were scanned, and band intensities were measured with Scion Image (Scion Corporation, Frederick, MD).

Immunostaining

SCG neurons were serum starved for one hour in DMEM:F12 and treated with vehicle or AMPH for ten or thirty minutes. Neurons were subsequently fixed with PBS Ca2+/Mg2+/4% paraformaldehyde, washed three times with PBS Ca2+/Mg2+, permeabilized and blocked with PBS/4% bovine serum albumin (BSA)/0.15% Tween-20, and immunostained with the appropriate antibody dissolved in PBS plus 4% BSA and 0.05% Tween-20. Primary antibodies were visualized with the appropriate covalently Alexa-labeled secondary antibody from Molecular Probes. Immunofluorescence was imaged by capturing Z-series using a Perkin Elmer UltraView confocal with a Nikon Eclipse 2000-U microscope equipped with a 60X lens with N.A.=1.49, or an Olympus FV 1000 using a 60X lens of N.A.=1.45 (VUMC Cell Imaging Shared Resource). All images shown are always from single confocal sections and image processing was performed using Image J and Adobe Photoshop.

Analysis of NET internalization

The quantitation of NET intracellular accumulation was achieved using pixel intensity plots of a single confocal section along a line through the center of each bouton using ImageJ. The line intersects the brightest spot on one side of the widest portion of the bouton perimeter. The line was extended beyond the limits of the bouton to generate a background value, and then divided into 20 bins (bin # 10 is approximately at the center of the bouton) for the pixels spanning the bouton. NET fluorescence intensity of each bin was normalized to the fluorescence intensity of the brightest spot on the bouton perimeter (100%) and the mean ± SEM pixel intensity of each bin plotted.

Colocalization analysis

Colocalization analysis was performed as previously described (Matthies et al., 2009) following protocols established by Stanley and coworkers (Li et al., 2004). Only images in which there was no pixel saturation were analyzed. Background fluorescence was first subtracted in ImageJ by selecting an unstained area of each image and running the background subtraction plugin available at http://www.uhnresearch.ca/facilities/wcif/fdownload.html. The intensity correlation quotient (ICQ) was then determined by running the intensity correlation analysis (ICA) plugin for ImageJ developed by Tony Collins and Elise Stanley (Toronto Western Research Institute), also available at the above link. The ICQ indicates whether the intensity of staining for two proteins varies in synchrony over space. An ICQ value of +0.5 means that in any pixel with a certain intensity of staining for one protein, the intensity of staining for the other protein studied will be exactly the same, while an ICQ value of 0 signifies no relation between the two staining patterns. An ICQ value of −0.5 indicates an inverse relationship for colocalization.

To determine and visualize sites of colocalization, software at the above link was used to generate images with psudocolored pixels and points where values for both antigens have pixel values above the mean (Li et al., 2004). In the software, these images are called +ves, and are images of pixels that have positive PDM values resulting from both pixels above the mean (i.e. red intensity-mean red intensity and green intensity-mean green intensity are both positive) http://www.uhnresearch.ca/facilities/wcif/fdownload.html.

Density gradient centrifugation

NET cells were homogenized in PBS (cleared of intact cells and nuclei by centrifugation at 1,000 × g for 10 minutes), and then mixed with OptiPrep (iodixanol) (Ford et al., 1994; Graham et al., 1994; Billington et al., 1998) to a final concentration of 13% and loaded into Sorvall Ultracrimp® tubes. Centrifuging at 60,000 rpm in a vertical 70V6 rotor in a Sorvall Ultra 80 produced a density gradient. 400 μL fractions were collected by displacement, and their density calculated from manually measured refractive indices (Ford et al., 1994; Graham et al., 1994; Billington et al., 1998). Proteins in all fractions were separated by SDS-PAGE and immunoblotted for NET, Rab11a, Rab4, Rab5 and/or Na/K ATPase, and an estimation of the relative intensity of the relevant protein per fraction were determined as described for biotinylation experiments. Relative amounts of a protein in each fraction were plotted as fractions relative to the total (sum of all fractions).

Results

In rat cortical slices, AMPH decreases surface NET

In initial studies using a heterologous expression system, we observed that AMPH caused trafficking of NET away from the plasma membrane (Dipace et al., 2007). Here, we used a brain slice preparation that contains noradrenergic terminals to test the hypothesis that AMPH reduces surface NET. Rat cortical brain slices were incubated either with vehicle or 10 μM AMPH for 30 minutes (Fig. 1A). We found that AMPH decreased the surface expression of NET. Shown are representative immunoblots of biotinylated and total fractions treated with either vehicle (CTR) or 10 μM AMPH (AMPH) (Fig. 1A). Quantitation of AMPH-induced NET surface expression demonstrates a significant decrease as compared to control (72.6 +/− 14.8). The biotinylated band intensities are normalized to total NET and expressed as percent of control conditions (Student’s t test, p≤0.05, n=13). Tyrosine hydroxylase (TH), a cytosolic protein, is detected primarily in the total fraction and comprises less than 1% of the surface fraction. In addition, representative Western blots of surface and total Na+/K+ ATPase levels are shown to serve as a loading control. Therefore, these data demonstrate that the biotinylated fraction represents cell surface proteins and speaks to the health of noradrenergic TH positive neurons in the assay. These studies suggest that AMPH treatment leads to a reduction of surface NET in noradrenergic projections in the context of a native environment.

Figure 1
Amphetamine leads to a decrease in surface NET expression in rat cortical slices and NET in boutons and colocalization of NET with Rab11a and Rab4

In boutons of SCGNs, AMPH increases cytoplasmic NET as well as NET and Rab11a colocalization

Since NE is predominantly released at presynaptic sites, we asked whether AMPH application modifies NET surface expression within single boutons (a presynaptic site) of differentiated SCG neurons. We recently developed a new rodent specific NET antibody allowing us to adopt mouse superior cervical ganglion neurons (SCGNs) as a model system to study NET distribution in boutons (Matthies et al., 2009). Some of the boutons of SCGNs are ideal for optical studies due to their large diameter (Matthies et al., 2009). These boutons (2-4 micron diameter) are active sites of membrane recycling likely including NE release, since high K+-induced depolarization leads to FM1-43 labeling (data not shown). Under basal conditions, NET is enriched at the perimeter of SCGN boutons (Fig. 1B, top two images). Application of AMPH (10 μM; 30 minutes) leads to increased NET immunoreactivity in the interior of the boutons (Fig. 1B, lower two images). These results indicate that AMPH causes internalization of NET in neurons.

We quantified these observations using pixel intensity plots of a single confocal section. For the image analysis, a straight line was drawn across the widest portion of each roughly spherical bouton, intersecting the brightest spot of fluorescence on one side of the bouton, and the fluorescence on the opposite side. The resulting line spanned the full diameter of the bouton, and was extended beyond the limits of the bouton to generate a background value (see methods). The line spanning the bouton was then divided into 20 bins (with bin #10 at approximately the center of the bouton), thereby binning the pixels spanning the bouton. The NET fluorescence intensity of each bin was normalized to the fluorescence intensity of the brightest spot (100%) and the mean ± SEM pixel intensity of each bin plotted. Figure 1C shows that in AMPH-treated neurons, intra-bouton fluorescence signal was significantly elevated over that found in untreated boutons, validating intracellular accumulation of NET with AMPH treatment (Two-way Anova, followed by Bonferroni post tests, *=P < 0.05, **=P<0.01, ***=P<0.001).

Our hypothesis is that AMPH leads to a reduction in NET plasma membrane expression via enhanced NET trafficking through endosomes. Our previous results (Dipace et al., 2007) suggested that the kinetics of NET internalization might be slow, implying the role of the slow recycling Rab11-positive pathway. Therefore, we analyzed NET colocalization with Rab11 as well as with Rab4, which is a marker for the late steps of the early endosome, after 30 minutes of AMPH exposure. One accepted function for both of these Rab proteins is their role for the recycling back to the plasma membrane. Here, we determined in SCGN boutons whether Rab4 and Rab11 colocalization with NET is altered by AMPH exposure (Fig. 1D-F). NET and Rab4 do not colocalize under basal (CTR) conditions (Fig. 1D, CTR, top row). However, AMPH increased colocalization between a subset of Rab4 organelles and NET (Fig. 1D, AMPH, bottom row). Next, we compared the distribution of NET and Rab11a in control and AMPH treated SCGN boutons (Fig. 1E). Although there is uncertainty as to whether Rab11a is present in presynaptic terminals (Sheehan et al., 1996), we detected the protein in SCGN boutons. Moreover, under control conditions, we observed colocalization between Rab11a and NET at the juxtamembrane region. Generally, Rab11a was found just cytoplasmic to the most abundant NET staining at the plasma membrane (see arrows, Fig. 1E, top row). The intensity of NET in the juxtamembrane region was much lower than on the perimeter of the bouton. In contrast, in AMPH treated neurons, we detected a significant increase in colocalization between NET and Rab11a in regions both in the center, just below, and on the perimeter of the bouton (see arrows, Fig. 1E, bottom row). These apparent AMPH-induced increases in colocalization between NET, Rab4 and Rab11a were quantitated using the ICA/ICQ method (Li et al., 2004) as described in Material and Methods (Fig. 1F). In the basal state, we determined that the ICQ value for NET and Rab4 is close to zero (−0.013 ± 0.03) indicating a random distribution. Upon AMPH treatment, the ICQ value is significantly increased (0.103 ± 0. 0.02; **=p ≤ 0.001; Student’s t test; n =12) indicating dependent staining of Rab4 and NET (Fig. 1F). Rab11a and NET displayed some colocalization under the basal state which was significantly enhanced (from 0.104 ± 0.02 to 0.205 ± 0.02) upon AMPH exposure (*p ≤ 05; Student’s t test; n=13) (Fig. 1F). To further support the presence of Rab11a at presynaptic terminals, we determined whether identified Rab11a effectors and binding proteins Rab11-FIP1, Rab-FIP2 and Rab11-FIP5 (Prekeris et al., 2000; Hales et al., 2001) are expressed at SCGN boutons. Rabs are GTPases that lead to specificity of membrane trafficking via the nature of the bound guanosine nucleotide. Rabs bind to effectors depending on the hydrolysis state of the bound nucleotide and the Rab/effector interaction leads to docking/tethering at fusion sites or vectorial transport using various cytoskeletal elements (Mellman, 1996; Robinson et al., 1996; Zerial and McBride, 2001; Maxfield and McGraw, 2004; Jordens et al., 2005; Grosshans et al., 2006). Here, we show (Fig. 1G-H) that several Rab11 effectors including Rab-FIP1, Rab11-FIP2 (data not shown), and Rab11-FIP5 are found in SCGN boutons, further supporting the functional presence of Rab11a and its signaling partners in presynaptic neuronal compartments. Rab11-FIP1, Rab11-FIP2 (data not shown), and Rab11-FIP5 were also detected just below the NET demarcated perimeter and in the interior of the bouton (Fig. 1G-H). Our results demonstrate that Rab11a and its effectors are present in these synaptic terminals, consistent with previous work which identified Rab11-FIP2 and Rab11-FIP5 as phosphoproteins in synaptosomes (Collins et al., 2005; Collins et al., 2008). In addition, these data provide support to our hypothesis that Rab11a coordinates AMPH-induced NET trafficking.

AMPH induced NET redistribution assessed by subcellular fractionation

We next sought biochemical confirmation of AMPH-induced NET trafficking through Rab11 positive compartments and returned to the NET-transfected CAD model (NET cells) to explore the distribution and density of NET membranes in the presence and absence of AMPH. AMPH-induced changes in NET cell surface expression were evaluated by biotinylation assays, and density gradient fractionation was used to ascertain both NET redistribution and colocalization with Rab11a. In NET cells, 10 μM AMPH induces a significant reduction in surface NET in 60 minutes (Dipace et al., 2007) and we confirmed this result at 30 minutes. Figure 2A shows representative immunoblots of biotinylated and total fractions from NET cells treated with either vehicle (CTR) or 10 μM AMPH (AMPH) for 10 and 30 minutes at 37° C. At 30 minutes, AMPH decreases plasma membrane expression of NET without changes in total NET. Figure 2B displays the quantitation of AMPH-induced decrease in NET surface expression. The biotinylated band intensities are normalized to total NET and expressed as percent of control conditions (Student’s t test, *=p≤0.05, n=7).

Figure 2
AMPH-induced loss of NET from the cell surface and redistribution to intracellular organelles characterized by cell fractionation

Next, we assessed the density of NET-containing organelles in relation to proteins for various subcellular oganelles using density gradient centrifugation (Optiprep medium). Figure 2C (top) shows representative immunoblots displaying the distribution of NET across the Optiprep gradient from NET cells treated either vehicle (CTR) or with 10 μM AMPH for 30 minutes (AMPH). In addition, an immunoblot of the plasma membrane marker Na+/K+ ATPase α subunit (Na+/K+ ATPase α subunit) is displayed. Figure 2D displays the quantitation of the intensity of NET immunoreactivity in each fraction. The density of the fractions is identified by diamond symbols and corresponds to the values on the right y axis. Fractions at lighter densities (1.02-1.06 g/mL) contained a substantial proportion of NET under control conditions (solid circles). Based on previous work, some of these lighter fractions likely contain plasma membranes (Graham, 2001). This was confirmed by the presence in these fractions of the Na+/K+ ATPase α subunit (open triangles). Upon treatment with AMPH, the distribution of NET displayed a shift to higher density fractions (1.07-1.13 g/mL) lacking the Na+/K+ ATPase α subunit and substantially decreased in a light plasma membrane fraction (open circles). We divided the gradient into 22 bins based on fraction density, allowing for the averaging of fractions from multiple experiments. Figure 2E represents the average of three experiments. Consistent with Figure 2D, AMPH (open circles) decreased NET intensity in the light plasma membrane fractions with respect to vehicle treated control (solid circles). Thus, NET in a light plasma membrane fraction (fraction #3) was decreased by ~60%, based on peak height, (Student’s t test, P≤0.01) from 7.6 ± 0.9101% in control conditions to 2.3 ± 0.6450% upon AMPH treatment.

AMPH increases colocalization of NET with Rab11a and Rab4

Figure Figure11 and and22 shows that AMPH causes cell surface redistribution of NET and increased colocalization of NET with Rab11a and Rab4. We have also demonstrated that under basal conditions, NET resides in Rab11a-positive compartments (Fig. 1) and that AMPH induces increased colocalization with both Rab4 and 11a positive structures (Fig. 1). We next sought to determine whether Rab11a is in the same density fractions as NET in vehicle or AMPH treated NET cells (Fig. 3). Therefore, immunoblots from density gradients from either vehicle or AMPH-treated NET cells were probed for Rab11a.

Figure 3
Rab11a-containing fractions have similar densities to NET-containing fractions in both control and AMPH-treated cells

Figure 3A shows immunoblots of Rab11a (top) for vehicle and AMPH treated cells, and quantitation of the intensity of the immunoreactivity of the different density fractions (bottom). Figure 3B represents an average distribution of Rab11a from three independent experiments. Rab11a exists in both low density and high density fractions (Fig. 3A). Upon AMPH treatment, Rab11a levels tend to increase in low density fractions (Fig. 3A, B), which have been shown by others to correspond to the densities of both early and recycling endosomes in Optiprep gradients (Herman et al., 1994; Sheff et al., 1999; Hashiramoto and James, 2000; Proikas-Cezanne et al., 2006). These data suggest that in addition to promoting colocalization of Rab11a and NET, AMPH also leads to the redistribution of both NET and Rab11a to fractions with similar density.

AMPH treatment also led to increased colocalization of NET with Rab4. Therefore, we probed gradient fractions for Rab4. Under control or basal conditions, a substantial portion of Rab4 was observed in high density fractions (Fig. 3c). Figure 3C shows a representative immunoblot of Rab4 from vehicle treated cells, and quantitation of the intensity of the immunoreactivity of the different density fractions (bottom). Roughly 60% of Rab4 is in fractions with high densities. In other cell types, the level of Rab4 at these high densities is below the threshold of detection (Proikas-Cezanne et al., 2006) whereas in cells expressing regulated secretory vesicles such as Glut4 storage granules and NET cells as shown here, two peaks of Rab4 are detected with a substantial peak at high densities (Herman et al., 1994; Hashiramoto and James, 2000; Lim et al., 2001).

AMPH treatment caused some redistribution of Rab11a (Fig. 3A, B) in density gradients but major effects on the distribution of Rab4 were not noted (data not shown). Rab4 is enriched in three peaks and NET is found in these fractions, particularly in the densest peak. Upon AMPH exposure, more NET tends to be in these dense fractions (Fig. 2D, E and Fig. 3C).

To better characterize the distribution of NET relative to markers of other well characterized trafficking compartments, we probed immunoblots of Optiprep gradients with antibodies against early endosome markers (Rab5) and late endosomes/lysosomes (LAMP1). The majority of Rab5 was present in possibly three peaks; one at a light density and two overlapping peaks at high density. The lighter peak does not coincide with a fraction with major changes in NET levels upon AMPH treatment. Indeed, AMPH-induced increases in NET are found in denser fractions with high levels of Rab5. In contrast, LAMP1 immunoreactivity was found in many fractions. However, the fractions where AMPH leads to enhanced levels of NET do not coincide with a LAMP1 peak fraction.

At early time points of AMPH treatment of SCGNs, NET is found adjacent to sites where clathrin and Rab11a colocalize

We have previously noted that concentrated NET puncta are preferentially found in sites with intense syntaxin1 immunoreactivity, and in domains marked by cholera toxin, which are most likely lipid rafts (Matthies et al., 2009). Interestingly, syntaxin 1 is also known to be partially found in lipid rafts (Chamberlain et al., 2001; Lang et al., 2001). Because syntaxin 1 is a protein with a major role in exocytosis, this data suggests that a fraction of NET is in exocytotic domains that are likely lipid rafts. We also note that, occasionally, Rab11a and Rab11-binding proteins are observed just below surface NET puncta at boutons (Fig. 1). Rab11, which we observe adjacent to NET puncta (Fig. 1), has been suggested to play a role in exocytosis at the stage of vesicle tethering (Zhang et al., 2004a; Zhang et al., 2004b; Jafar-Nejad et al., 2005; Langevin et al., 2005; Lim et al., 2005; Savina et al., 2005; Wu et al., 2005; Oztan et al., 2007). In addition to a possible function in exocytosis, Rab11 could also function in endocytosis. Importantly, distinct endocytic sites not overlapping with exocytotic domains have been described in presynaptic areas (Roos and Kelly, 1999). Therefore, we compared the distribution of NET with a marker of endocytic sites (clathrin) and with Rab11a in SCGN boutons (Fig. 4).

Figure 4
At early time points of AMPH treatment of SCGNs, Rab11a colocalizes with clathrin at juxtaplasma membrane and internal sites

We exposed SCGNs cultures to 10 μM AMPH for 10 minutes to observe NET early trafficking events, thereby allowing us to determine the distribution of “exocytotic and endocytic sites” in the same bouton. Figure 4A shows triple stained confocal sections for NET, clathrin, Rab11a, and triple merged, spanning an entire half of several boutons starting from the center (left) and scanning to the bottom (right). These images demonstrate that AMPH-treated boutons show both NET puncta at the plasma membrane (Fig. 4A, top row) and clathrin clustering. This is suggestive of clathrin assembly at presynaptic sites (Fig. 4A, second row). In order to determine if plasma membrane NET resides in the clathrin endocytic domains, we merged NET and clathrin images in Figure 4B (top row). We found low but significant colocalization of NET and clathrin (*=p≤0.05, n=17) and clathrin tended to surround surface NET puncta. These sites of colocalization between NET and clathrin were visualized computationally as outlined in Material and Methods by displaying an image in which pixels for both antigens have pixel values above the mean (Fig. 4C, bottom row left column; +ve). Most intense sites of colocalization approach white whereas sites with low values are black. Colocalization at one site is marked by an arrowhead in the NET, clathrin merge, and +ve images, respectively.

In Figure 1 we show that 30 minutes of AMPH increased colocalization between Rab11a and NET, and that this increase occurs in part at the plasma membrane in juxtamembrane regions. To determine whether this colocalization occurs at the early time points of AMPH-induced NET trafficking, we merged NET and Rab11a images after 10 minute of AMPH exposure. Figure 4B second row shows modest colocalization between these two proteins at the membrane, in contrast to what we observed after 30 minutes of AMPH. The question remained whether clathrin and Rab11a colocalized in endocytic compartments. Figure 4B (bottom row) shows high colocalization between clathrin and Rab11a. Furthermore, we detected overlap of Rab11a and clathrin on internal sites which may suggest clathrin assembly on endosomes (van Dam and Stoorvogel, 2002; van Dam et al., 2002; Pagano et al., 2004). All sites of colocalization of Rab11a and clathrin were further visualized by displaying an image with pseudocolored pixels at points where values for both antigens (Rab11a and clathrin) have pixel values above the mean (Li et al., 2004) (Fig. 4C, bottom row of right column; +ve) as above for NET and clathrin. Most intense sites of colocalization approach white whereas sites with low values are black. Colocalization at one site is marked by an arrowhead in the Rab11a, clathrin merge, and +ve images, respectively. In addition, there was also diffuse Rab11a staining that did not overlap with clathrin (Fig. 4B note blue in bottom row). At this early time point, intra-bouton NET immunoreactivity appeared in a diffuse pattern consistent with endocytic vesicles, which typically have diameters in the 40-70 nm range. We determined overlap in these two images in the bottom row of panel C of Figure 4 as outlined in Materials and Methods (data not shown), indicating some sites exist where both NET/clathrin or Rab11a/clathrin colocalization overlap (Figure 4C, arrowhead indicates a site). These sites are where NET colocalizes with Rab11a and appear to be endocytic sites as indicated by the presence of clathrin.

A fraction of NET and Rab11a sort to the same organelles

To test the hypothesis that Rab11a is required for the delivery of NET to the recycling endosome, we overexpressed a Rab11a fusion protein in order to perform live cell imaging. It has been shown that the overexpression of Rab11a can lead to the co-accumulation of Rab11a and some endocytic cargo in recycling endosomes, which are typically found in a perinuclear position (Ullrich et al., 1996; Ren et al., 1998). Therefore, if NET traffics via the recycling endosome, overexpression of Rab11a in CAD cells should lead not only to the colocalization of NET and Rab11a, but also their synchronous movement in living cells. We expressed GFP-NET together with RFP-Rab11a (see Material and Methods) and found that the overexpression of Rab11a leads to GFP-NET and RFP-Rab11a accumulation in a perinuclear position (Fig. 5A, top row, see arrows). Importantly, in axonal swellings both GFP-NET and RFP-Rab11a were observed in organelles with similar morphologies (Fig. 5A, bottom row, see arrows). In addition, we noticed diffuse NET and Rab11a both in the cell bodies and at axonal swellings (Fig. 5A, B). In time series it was evident that doubly labeled organelles move in synchrony, establishing that both NET and Rab11a can be sorted to the same organelle in living cells (Fig. 5B, arrows).

Figure 5
NET and Rab11a colocalize in living CAD cells

Previous studies have shown that Rab11a is required at multiple levels during the trafficking of cargo, including the delivery of endocytic cargo to recycling endosomes, and from the recycling endosome to the plasma membrane (Ullrich et al., 1996; Ren et al., 1998; Wang et al., 2000; Hales et al., 2002; Schonteich et al., 2008). This was demonstrated mainly by disrupting Rab11a function (Ullrich et al., 1996; Ren et al., 1998; Wang et al., 2000; Hales et al., 2002; Schwenk et al., 2007; Welsh et al., 2007; Schonteich et al., 2008; Eggers et al., 2009). To discriminate between the different roles of Rab11a in the trafficking of NET, we used a truncated version of the Rab11 effector protein Rab11-FIP2 lacking the C2 domain (FIP2ΔC2) (Hales et al., 2002). The C2 domain of FIP2 may have lipid binding activity (Lindsay and McCaffrey, 2004). Expression of FIP2ΔC2 severely disrupts Rab 11 distribution and plasma membrane recycling (Hales et al., 2002; Lindsay and McCaffrey, 2002; Fan et al., 2004; Lindsay and McCaffrey, 2004). It was previously shown that FIP2ΔC2 accumulates in the recycling endosome (Hales et al., 2002). Therefore, the prediction is that if the earliest disrupted step requiring Rab11 is the delivery of NET to the plasma membrane, then NET and FIP2ΔC2 will colocalize in the recycling endosome. Importantly, if the earliest Rab11 requirement is delivery of NET to the recycling endosome, then NET and FIP2ΔC2 should not colocalize. Therefore, we examined live CAD cells expressing GFP-NET and either mCherry-FIP2ΔC2 or mCherry alone. In control cells, GFP-NET was found in the cell body (Fig. (Fig.5,5, ,6),6), axonal swellings (Fig. 6) and neurites (Fig. 4). In axonal swellings, we detected NET on the surface and in the interior (Fig. 6A). A fraction of NET had a diffuse appearance (Fig. 6A). We also saw regions of concentrated NET-bearing organelles which moved in partial synchrony over time, particularly in the center of axonal swellings (Fig. 6A t=0 to t=3). In these highly concentrated regions of GFP-NET, we noted lower densities of mCherry strongly suggesting that mCherry does not randomly colocalize with GFP-NET.

Figure 6
GFP-NET and FIP2ΔC2 do not colocalize in living CAD cells

In order to determine whether Rab11a has a role in delivering NET to the recycling endosome, we asked if GFP-NET and mCherry-FIP2ΔC2 colocalize (Fig. 6B). We double transfected CAD cells with vectors to induce the expression of both proteins. We found that in swellings, the organelles containing either GFP-NET or mCherry-FIP2ΔC2 appear to have different morphologies and motile properties (Fig. 6B). We also observed a low level of diffuse staining for both proteins, which we interpret as the presence GFP-NET and mCherry-FIP2ΔC2 on small organelles (Fig. 6B). In addition, we noted larger motile fluorescent organelles containing either NET or FIP2ΔC2 which moved independently, suggesting that the majority of NET and FIP2ΔC2 are in distinct organelles (Fig. 6B). Occasionally, organelles with FIP2ΔC2 appeared to track the inner surface of the plasma membrane (Fig. 6B). Additionally, the expression of FIP2ΔC2 appeared to increase GFP-NET found at the surface of the axonal swellings (Fig. 6B) as compared to control (Fig. 6A, top row).

In the cell bodies, occasionally a minor fraction of GFP-NET was observed in perinuclear, FIP2ΔC2-rich domains (Fig. 6C, arrow), but as in the axonal swellings, the distributions were distinct as were the motile properties (Fig. 6C). Altogether, the results from both cell bodies and boutons suggest that Rab11 is required for delivery of NET to the recycling endosome.

Coexpression of Rab11a/b mutants and NET modifies the subcellular distribution of NET

AMPH treatment leads to colocalization of NET and Rab11a-positive organelles (Figs. (Figs.1,1, ,2,2, ,3).3). Furthermore, NET is sorted to RFP-Rab11a enriched organelles (Fig. 6) and expression of FIP2ΔC2 leads to increased cell surface NET levels and possibly prevents NET entry into recycling endosomes. FIP2 binds and therefore can sequester all Rab11 family members. This includes Rab11a, Rab11b and the endothelial expressed Rab25 (Hales et al., 2001). However, Rab25 is not expressed in neuronal cells (Goldenring et al., 1993). Therefore, to determine whether the other two neuronal Rabs members (Rab11a/b) contribute to NET trafficking, we expressed GFP-NET with various mutants of Rab11a and Rab11b. Expression of mutants of Rab11 in a neuroendocrine cell line, inhibits regulated exocytosis (Khvotchev et al., 2003). Curiously, it has been shown that Rab11 isoforms can differentially regulate the trafficking of a particular cargos despite 90 % identity between Rab11a and b and can have different subcellular distributions (Khvotchev et al., 2003; Lapierre et al., 2003; Delisle et al., 2009; Silvis et al., 2009).

Therefore, we coexpressed GFP-NET with three different mutants of Rab11 in CAD cells to determine if they colocalize and/or alter the distribution of NET in varicosities (Fig. 7). We used Rab11s with either GTP bound (S20V), or GDP bound (S25N). Also, we took advantage of another mutant that may only interact with a subset of Rab11 effectors (S29F). These proteins have previously been used to demonstrate the requirement of Rab11 in the transferrin receptor trafficking (TfnR) (Ullrich et al., 1996; Ren et al., 1998; Pasqualato et al., 2004). All mutant forms for both Rab11a and Rab11b tended to modify NET cellular distribution. Wild type, S20C, and S29F colocalize with NET in juxtamembraneous organelles as well as in internal organelles (Fig. 7A). These results confirm our observation obtained with Rab11 SCGNs (Fig. 1). Importantly, S25N was noted to colocalize with NET predominantly in internal organelles (Fig. 7A). Although, all mutant versions of Rab11a tended to increase colocalization with NET with respect to wild type only Rab11a S20V reached significance (Student’s t test, *=p≤0.05) (Fig. 7A,C). Coexpression of wild type and Rab11b mutants also led to colocalization with NET at juxtamembrane and intracellular domains. However, in contrast to Rab11a S29F, the Rab11b S29F version demonstrated significantly less colocalization with NET with respect to wild type (Student’s t test, *=p≤0.05) (Fig. 7B,C). Rab11b S25N did not have a tendency for higher colocalization with NET compared to wild type Rab11b but as for Rab11a, this colocalization was predominantly on intracellular organelles. Rab11b tended to be highly enriched in varicosities but with a diffuse distribution consistent with the notion that it has a role in synaptic vesicle metabolism (Khvotchev et al., 2003). Expression of Rab11b generated less colocalization with NET than Rab11a. In addition, identical mutations in Rab11a/b showed different responses, suggesting that they might utilize different effectors to modify NET distribution. Nonetheless, these data suggest that Rab11a/b regulate NET trafficking, as mutations in these Rabs change NET/Rab11 colocalization.

Figure 7
Mutants of Rab11a/b modify GFP-NET intracellular distribution

In nonpolarized and polarized cells, NET responds to Rab11 mutants in a different manner than the TfnR. The TfnR exhibits a peripheral distribution and does not colocalize with Rab11a S25N whereas it does colocalize with Rab11a S29F (Ullrich et al., 1996; Ren et al., 1998; Pasqualato et al., 2004). Furthermore, the TfnR colocalizes with FIP2ΔC2 and the sum of these experiments has led to the most accepted hypothesis that Rab11 plays a role in trafficking from the recycling endosome to the plasma membrane. Therefore we decided to compare the distribution of NET and the TfnR in our CAD cells. We coexpressed tagged versions of NET and FIP2ΔC2, Rab11a S25N and Rab11a S29F and allowed fluorescent transferring (Tfn) to equilibrate with internal TfnR. Then, we determined colocalization of NET and the relevant Rab interfering protein, NET and Tfn, and finally Rab-interferring protein and Tfn. For these studies we only used cell bodies since we only detected extremely low levels of Tfn in varicosities as expected (Cameron et al., 1991). As we determined in the varicosities, NET localized with Rab11a S25N and Rab11a S29F but not with FIP2ΔC2 (Fig. 7D, E). Surprisingly, Tfn did colocalize with all three Rab11 interferring proteins and most strongly with FIP2ΔC2 (Fig. 7D, E). These studies suggest that Rab11 function differentially delivers cargo (e.g. NET vs Tfn) to recycling endosomes and apparently TfnR trafficking in neurons diverges somewhat from other cell types.

NET trafficking involves Rab11a function

AMPH causes NET trafficking away from the plasma membrane leading to the enrichment of NET in Rab11a compartments (Fig. (Fig.22--4).4). Rab11a plays a role in the trafficking of cargo from sorting endosomes to recycling endosomes and from recycling endosomes to the plasma membrane (Ullrich et al., 1996; Ren et al., 1998; Wang et al., 2000). We hypothesized that AMPH-regulated NET trafficking is supported by Rab11a. To test this hypothesis, we disrupted Rab11a function by expressing FIP2ΔC2 and then measured the consequence of this on AMPH-induced NET trafficking. First, we transfected NET cells with FIP2ΔC2 tagged with mCherry and, as expected, the majority of FIP2ΔC2 was found in a perinuclear distribution (Fig. 8A, top panels) (Hales et al., 2002). Next, we determined whether Rab11a is involved in AMPH-induced NET trafficking by transfecting NET cells with FIP2ΔC2 and exposing them to AMPH for up to 30 minutes. Figure 8B shows that the proportion of NET on the cell surface (biotinylated fraction) of vector transfected NET cells noticeably decreased upon 30 minute AMPH treatment compared to vehicle-treated controls. AMPH did not affect the level of total NET. In contrast, expression of FIP2ΔC2 strongly reduced the ability of AMPH to cause NET internalization after 30 minutes (Fig. 8B). Quantitative analysis demonstrated that significant AMPH-induced internalization occurred in control vector-transfected cells (filled bars), while we observed no effect of AMPH on surface levels of NET in FIP2ΔC2 transfected cells (open bars) at 10 minute or 30 minute of AMPH exposure (Fig. 8C). Data were normalized to total NET protein and expressed as percent of the respective vehicle treated controls (Student’s t test; *=p≤0.05). These results suggest that AMPH-regulated NET trafficking requires functional Rab11a.

Figure 8
Expression of FIP2ΔC2 in NET cells prevents AMPH-induced internalization of NET

Rab11 and Rab4 can facilitate the delivery of vesicles to certain compartments (Zerial and McBride, 2001). Rab4 typically controls traffic from the early endosomes to the plasma membrane (van der Sluijs P, 1992). Because NET accumulates in Rab4-positive sites with AMPH treatment (Fig. 4A-D and 5A-B), we suspected that NET may be returned to the cell surface through this Rab4-regulated pathway. To address this question, we disrupted Rab4 function by transiently transfecting either the GDP-locked version of the protein, Rab4 S22N (DN Rab4), or empty vector into NET cells and compared AMPH-induced internalization by cell-surface biotinylation. Consistent with our previous results, both 10 and 30 minute treatments with 10 μM AMPH decrease surface expression of NET in empty vector-transfected cells, while NET surface expression did not significantly change in cells transfected with DN Rab4 (data not shown). This result implies that AMPH-regulated NET trafficking does indeed involve both Rab11 and Rab4.

Discussion

NET is responsible for the reuptake of synaptically released NE, and therefore plays an important role in regulating the fidelity of NE signaling and the maintenance of NE levels (Xu et al., 2000; Bonisch and Bruss, 2006). NE homeostasis is regulated both by modifying the NET turnover rate and the number of active NETs at the plasma membrane. Thus, it is possible that the level of functional NET is fine-tuned to the appropriate degree depending on physiological requirements. Importantly, NET trafficking may be one critical mechanism for the dynamic control of NE homeostasis. An example of altered NE homeostasis as a result of misregulation of NET surface levels is demonstrated by a polymorphism in human NET (F528C NET). This polymorphism was originally found in a screen for candidate genes associated with blood-pressure homeostasis (Halushka et al., 1999) and recently linked to major depression (Haenisch et al., 2008). The expression of F528C NET in a heterologous system displays elevated plasma membrane levels (Hahn et al., 2005). F528C NET shows reduced trafficking away from the plasma membrane in response to phorbol esters and activation of PKC (Hahn et al., 2005). In this context, it is interesting to note that PKC has been suggested to be essential for the entry of endocytosed cargo into recycling endosomes (Becker and Hannun, 2003), and that F528C NET has been linked to brain disorders (Haenisch et al., 2008). Another important aspect of NET trafficking is that NET has been linked to the actions of psychostimulants such as AMPH. AMPH decreases NET plasma membrane expression and affects NE homeostasis, possibly leading to vasculitis, neuropsychiatric abnormalities, and cardiomyopathy (Furst et al., 1990; Varner et al., 2002; Pozzi et al., 2008). One possibility is that AMPH reduces NET surface expression by shifting a portion of NETs into a recycling endosomal compartment. Therefore, it is imperative to understand the molecular underpinnings of the regulation of NET trafficking, and whether the recycling endosome is involved in this process. Here, our intent is to further clarify the mechanisms and membrane compartments utilized by AMPH to alter NE homeostasis.

The main goals of this study were to determine whether AMPH treatment leads to NET internalization in neurons and single boutons, whether this internalization is via Rab11 or Rab4 compartments, and whether this internalization requires Rab11 function. We chose to perform these experiments using a) brain slices allowing us to determine if AMPH modifies NET surface levels in the context of CNS circuitry; b) SCGNs permitting single bouton analysis; and c) a catecholaminergic neuronal cell line useful for genetic manipulation and biochemistry. We found that AMPH does promote a reduction in surface NET in cortical brain slices indicating that AMPH can reduce neuronal NET in a “native” environment (Fig. 1). AMPH treatment also led to an intracellular accumulation of NET and sorting of NET to Rab11- and Rab4-positive compartments (Figs.(Figs.1,1, ,2,2, ,3).3). Blocking Rab11 function with a truncated Rab11 binding protein (FIP2ΔC2) led to apparent elevation of NET on the plasma membrane, prevented entry of NET to recycling endosomes, and prevented AMPH-induced internalization (Figs. (Figs.66,,8).8). Trapping Rab11a/b in either the GDP or GTP state by using the Rab11 S20V and S25N mutants respectively, altered NET trafficking as does a mutation (S29F) which modifies Rab11’s ability to traffic cargo in the usual manner (Pasqualato et al., 2004). This mutant alters the distribution of an early endosome marker, as does FIP2ΔC2 (Hales et al., 2002; Pasqualato et al., 2004). Rab11 and Rab4 also colocalize on a subset of endosomes (Sonnichsen et al., 2000), and recently a protein (D-AKAP/AKAP10) binding both Rab4 and Rab11 has been identified (Eggers et al., 2009). In this context, we suggest that Rab11 facilitates NET entry into recycling endosomes.

Under basal conditions, at the level of synaptic boutons, we observed Rab11a and interacting proteins just below the plasma membrane and on internal organelles with modest colocalization of NET and Rab11a (Fig. 1). At early time points after AMPH treatment (10 minutes), we noticed potential sites of clathrin assembly in domains adjacent to the NET enriched domains (Fig. 4). Based on our previous work, these domains maybe be lipid rafts (Matthies et al., 2009). Curiously, juxtaplasma membrane Rab11a tended to be detected at sites of clathrin enrichment (Fig. 4). In the interior of the bouton, clathrin also appeared to accumulate on internal organelles, and again Rab11a was detected at these sites (Fig. 4). Importantly, at early time points NET appeared to be in a diffuse intra-bouton pattern (Fig. 4), and after sustained activation of endocytosis by AMPH, we detected NET in larger Rab11a positive organelles (Fig. 1). Our gradient analysis of NET redistribution uncovered that NET internalization occurs predominantly from a subset plasma membrane fractions with a rather low density. Based on our previous study, we suggest NET is initially found in lipid rafts (Matthies et al., 2009). Therefore, it is possible that that the low density plasma membrane fraction that showed preferential AMPH-induced NET loss is enriched for lipid rafts. During the earliest time points of this process, Rab11a may intersect with the NET endocytic cycle at the clathrin coated pit stage. This stage occurs in non-lipid raft domains as opposed to clathrin independent endocytosis as discussed by Donaldson et al. (Donaldson et al., 2009). In support of this hypothesis, purified clathrin coated vesicles contain Rab11a (Blondeau et al., 2004; Borner et al., 2006), suggesting that Rab11a might remain on endocytic vesicles containing NET.

In these studies, we demonstrated that AMPH-induced (30 minute) NET delivery to recycling endosomes requires Rab11 function (Fig. 8). The main consequence of suppressing Rab11 function by FIP2ΔC2 is that NET entry into recycling endosomes is modified. Expression of FIP2ΔC2 leads to its accumulation with Rab11 and myosin at the recycling endosome (Hales et al., 2002; Lindsay and McCaffrey, 2002; Fan et al., 2004; Lindsay and McCaffrey, 2004). We speculate that the level of available, non-mislocalized Rab11 is below a threshold, and therefore, NET fails to be delivered to the recycling endosome. Thus, the expression of FIP2ΔC2 might increase NET plasma membrane expression, as under these conditions, the fraction of NET normally entering the endosome recycling system now localizes to the plasma membrane. This might explain why the expression of FIP2ΔC2 inhibits AMPH-induced trafficking. This role for Rab11 has been previously suggested for other endocytosed membrane proteins (Ullrich et al., 1996; Ren et al., 1998). Consistent with this hypothesis, knockdown of several Rab11 effectors leads to observations that strengthen this novel role of Rab11 (Schwenk et al., 2007; Welsh et al., 2007; Schonteich et al., 2008; Eggers et al., 2009). Indeed, knockdown of Rab11FIP5 leads to faster recycling and higher cell surface expression of membrane proteins (Schonteich et al., 2008). Similar results were obtained by knocking down a recently identified effector (D-AKAP2/AKAP10) of both Rab4 and Rab11 protein (Eggers et al., 2009). Finally, knockdown of Rab11FIP2 and FIP5 leads to increased cell surface levels of the glucose transporter (GLUT4) (Schwenk et al., 2007; Welsh et al., 2007; Schonteich et al., 2008; Eggers et al., 2009).

To further probe the role of Rab11 in NET trafficking by an alternative approach, we tested the effects of several mutants of Rab11a/b on NET trafficking. These mutants alter the preferred bound nucleotide acting as constitutively active (S20V) or dominant negative mutant (S25N). Analysis of NET distribution in cells expressing these mutants also point to the hypothesis that Rab11a/b has a role in delivering NET under basal conditions to recycling endosomes (Fig. 7). We also tested a Rab11 mutant (S29F) that alters the distribution of early endosome markers as does FIP2ΔC2 (Hales et al., 2002; Pasqualato et al., 2004). These mutants affect the basal distribution of NET (Fig. 7). These data also point out a differential regulation of NET basal trafficking by Rab11a and Rab11b (Fig. 7). Curiously, knockdown of Rab11FIP and D-AKAP2/AKAP10 also alters the distribution of early endosome markers, as well as increase cell surface expression of membrane proteins (Schwenk et al., 2007; Welsh et al., 2007; Schonteich et al., 2008; Eggers et al., 2009).

Unlike NET, TfnR trafficking has been shown not to be affected by AMPH (Boudanova et al., 2008). Consistent with these observations, expression of FIP2ΔC2, Rab1 S25N and Rab11a S29Fdifferentially affects TfnR and NET trafficking. This implies that NET utilizes a pathway that is either not used by TfnR or TfnR trafficking can use alternative pathways to get to the endosomal recycling compartment particularly in the presence of FIP2ΔC2.

NET and Rab4 colocalize on a subset of endosomes upon AMPH treatment. However, expression of a GDP-locked version of Rab4 inhibits AMPH-induced intracellular accumulation of NET. Although this is surprising, Zadeh and colleagues have shown that the Kv1.5 channel increases plasma membrane levels when a dominant negative Rab4 mutant is expressed (Zadeh et al., 2008). Consistent with our new role of Rab4 in NET trafficking, it has been shown that GLUT4 and the serotonin transporter also do not respond as predicted by the standard Rab4 model to Rab4 mutant expression (Cormont et al., 1996; Ahmed et al., 2008).

Rab11 and Rab4 colocalize on a subset of endosomes (Sonnichsen et al., 2000). In this context, another possibility is that Rab11 can facilitate NET entry into the recycling endosomes, possibly in concert with Rab4 (Eggers et al., 2009). This would be consistent with observations that perturbations of Rab11 function can lead to changes in the distribution of the early endosome Rab5 effector EEA1 (Hales et al., 2001; Pasqualato et al., 2004; Schwenk et al., 2007; Welsh et al., 2007; Schonteich et al., 2008; Eggers et al., 2009).

These studies demonstrate for the first time AMPH induced trafficking of a neurotransmitter transporter within a native brain environment and in a single bouton and show that both Rab11a/b and Rab4 regulates NET trafficking. These results suggest that these Rabs may thus be involved in modulating NE homeostasis.

Acknowledgements

We would like to thank Nicole Bibus-Christianson for excellent lab oversight, and members of the Galli, Blakely and Goldenring labs for helpful discussions. We would also like to acknowledge the use of the Olympus FV1000 of the VUMC Cell Imaging Shared Resource.

Sponsorship: Work supported by NIH Grants DA13975 (A.G.).

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