|Home | About | Journals | Submit | Contact Us | Français|
The live-attenuated yellow fever vaccine (YF17D) is one of the safest and most effective vaccines available today. Here, YF17D was genetically altered to express the circumsporozoite protein (CSP) from the murine malarial parasite Plasmodium yoelii. Reconstituted recombinant virus was viable and exhibited robust CSP expression. Immunization of naïve mice resulted in extensive proliferation of adoptively transferred CSP-specific transgenic CD8+ T cells. A single immunization of naïve mice with recombinant YF17D resulted robust production of IFN-γ by CD8+ T cells and IFN-γ and IL-2 by CD4+ T cells. A prime-boost regimen consisting of recombinant virus followed by a low dose of irradiated sporozoites conferred protection against challenge with P. yoelii. Taken together, these results show that recombinant YF17D can efficiently express CSP in culture, and prime a protective immune response in vivo.
Malarial parasites infect an estimated 250 million people each year, resulting in up to 1 million deaths annually, mostly children less than 5 years of age. The morbidity, mortality, and socioeconomic burden of endemic malaria make it one of the most devastating infectious diseases and an urgent public health problem. Additionally, malaria is a significant threat to individuals traveling to endemic countries. No vaccine is available, and emerging resistance to antimicrobial drugs and insecticides is cause for increasing concern.
Current vaccine efforts aim to intervene at various stages of the parasite life cycle. Pre-erythrocytic vaccines, such as irradiated sporozoites, endeavor to prevent initial infection. Since the late 1960s, irradiated sporozoites have been known to confer sterile immunity to mice and remain the gold standard for immunogenicity studies . In the 1970s, analogous studies of irradiated P. falciparum and P. vivax sporozoite vaccination in humans yielded promising results . Unfortunately, effective immunization requires administration of large numbers of the inactivated parasites delivered intravenously or by mosquito bites. Since a practical route of delivery has yet to be developed, irradiated sporozoite immunization is still experimental.
The main protective antigen of irradiated sporozoites is the circumsporozoite protein (CSP) , which coats the surface of the sporozoite and it is thought to be involved in parasite development and binding to hepatocytes [4, 5]. Several attempts have been made to induce immunity against Plasmodium spp. by delivery of CSP or CSP-derived peptides. The most promising candidate is a recombinant protein vaccine, named RTS,S/AS02A (GlaxoSmithKline Biologicals [GSK]) . RTS,S/AS02A consists of a portion of CSP fused to the hepatitis B virus surface antigen and is formulated with the AS02A adjuvant (GSK), an oil- in- water emulsion containing the immunostimulants QS21 (a triterpene glycoside purified from the bark of Quillaja saponaria) and 3D- MPL (3- deacylated monophosphoryl lipid A [MPL]) [6, 7]. RTS,S/AS02A recently completed Phase 2b clinical trials with a vaccine efficacy against new infection of 65.9% [8, 9]. The RTS,S/AS02A vaccine is still under evaluation, and the results from Phase III trials remain to be determined.
Unlike malaria, a highly safe and effective vaccine exists to protect against yellow fever virus (YFV). The YFV vaccine, a live attenuated virus termed YF17D, has been used to successfully immunize more than 400 million people since 1937 . The vaccine has minimal side effects and appears to provide life-long immunity in the majority of recipients after a single subcutaneous dose; neutralizing antibodies can be detected in some individuals more than 30 years after vaccination . The success of YF17D as a vaccine and vaccine vector is thought to be due to the ability of the attenuated virus to infect and activate professional antigen presenting cells (APCs) at the site of inoculation. YF17D productively infects human dendritic cells (DCs), the most specialized APCs, and these DCs can process and present foreign antigens to CD8+ T-cells [12-14]. YF17D is also known to activate DCs by signaling through multiple tolllike receptors (TLRs), specifically TLR 2, 7, 8, and 9 [12, 13]. Activated DCs then travel to the nearest lymph nodes, where they stimulate T-lymphocytes to initiate adaptive immune responses.
Previous studies have shown that YF17D can be engineered to express heterologous antigens and can induce protective immune responses against the inserted epitopes. Clinical studies of YF17D-based chimeric vaccine candidates targeting dengue type 2 virus, Japanese encephalitis virus (JEV), and West Nile virus (WNV) are currently underway [15-19]. Recombinant YF17D has also been used to express malarial sequences. YF17D encoding a single short Plasmodium T-cell epitope is a potent inducer of cytotoxic T lymphocyte (CTL) responses and can significantly reduce parasite burden in the mouse liver when followed by a large dose (104) of irradiated sporozoites .
Here, we have attempted to enhance CTL and humoral responses against Plasmodium by further exploring the potential of the YFV vaccine vector. A new recombinant YF17D virus expressing the P. yoelii CSP, including several B- and T-cell epitopes, was constructed and characterized in vitro and in vivo. Here, CSP was inserted in a previously unexplored site within the YF17D genome. Immunization of adult mice with the recombinant YFV resulted in robust CTL responses in the liver and spleen of immunized animals and, when followed by a low-dose boost with irradiated sporozoites, conferred protection to homologous challenge.
Plasmids were constructed using conventional molecular cloning techniques. Constructs were verified by restriction enzyme digestion and sequencing of PCR-amplified segments. Primers and vector sequences are available upon request. To create the YF17D vaccine vector, the full-length viral genome cDNA  was cloned into the copy control pCC1 (blunt cloning-ready) vector (Epicentre Biotechnologies) according to the manufacturer's recommendations. The resulting pCC1-YF17D plasmid is maintained as a single copy in Escherichia coli, strain EPI300, and upon addition of an induction agent, the clone is amplified to high copy numbers (Epicentre Biotechnologies). pCC1-YF17D was then modified for insertion of foreign sequences in frame with the YF17D open reading frame (ORF). First, a PCR product containing the restriction sites SrfI, SacI, KpnI, HpaI and NsiI was generated and cloned into pNEB193 (New England Biolabs) via SfoI and PmeI to construct a shuttle plasmid, pNEB193/link. An 887 base pair (bp) sequence including the 5′NTR and the first 75 bp of the capsid gene was amplified from pCC1-YF17D and cloned into pNEB193/link via the SrfI and SacI restriction sites to generate pNEB-C25, this plasmid contains a conserved region of YF17D that is important for genome cyclization during virus RNA replication . Second, a 273 bp segment encoding an AgeI site, followed by foot and mouth disease virus (FMDV) 2A and mammalian ubiquitin (Ubi) sequence, was assembled with a 1548 bp region of YF17D, encompassing the capsid, prM, and part of the envelope gene to the NsiI site. The first 75 bp of capsid in this context was recoded using assembly PCR to prevent duplication of the cyclization sequence. The cassette was cloned into pNEB-C25 using AgeI and NsiI to create pNEB-C25/2A-Ubi/mdC, a final shuttle plasmid harboring SacI and AgeI cloning sites for insertion of foreign sequences in frame between the truncated capsid sequence and FMDV 2A-Ubi cassette. A mammalian codon-optimized version of the circumsporozoite (CS) gene from Plasmodium yoelii, encompassing nucleotides (nt) 172-1034, was amplified from pDEC-PyCS  with the addition of SacI and AgeI sites, and inserted into pNEB-C25/2A-ub/mdC. From pNEB-C25/2A-Ubi/mdC, the YF17D/PyCS cassette was removed and cloned into pCC1-YF17D via SrfI and NsiI to generate pCC1-YF17D/PyCS.
Five to six week old adult female BALB/c mice were obtained from Jackson Laboratories. Transgenic H2Kb/d -mice expressing CD8+ T cells restricted for the P. yoelii CSP epitope SYVPSAEQI were kindly provided by Dr. Fidel Zavala . All animal experimental procedures were reviewed and approved by the Institutional Animal Care and Use Committee at The Rockefeller University.
Baby hamster kidney (BHK J) cells were maintained at 37°C and 5% CO2 in minimum essential medium (MEM) containing 7.5% fetal bovine serum (FBS), 0.1 mM non-essential amino acids, and 100U penicillin-100 μg streptomycin (GIBCO). Bone marrow-derived dendritic cells (BmDC) were generated as described previously . Briefly, tibias and femurs were cleared of connective tissue, and bone marrow was flushed into complete medium (RPMI 1640 containing 5% FCS, 50 μM 2-ME, 2 mM glutamine, 0.1 mM nonessential amino acids, and 100U penicillin-100 μg streptomycin) (GIBCO) using a 27G needle and a 10 ml syringe. Cells were centrifuged for 5 min at 1500 rpm and resuspended in 1 ml (per mouse) of red blood cells lysis buffer (GIBCO). After incubation for 2 min at room temperature, 25 ml of complete medium were added and the cells were centrifuged for an additional 5 min at 1500 rpm. Cells were resuspended in 3 ml of complete medium, filtered through a 40 μm filter and counted. The cells were cultured in 24-well plates (2×106 cells per ml) in the presence of murine GM-CSF (20 ng/ml) (Peprotech) and IL-4 (3 ng/ml) (R&D System). Fresh cytokines and media were added on day 2 and on day 4. On day 7, most of the cells had acquired typical dendritic morphology, and these cells were used as the source of DC. Splenocytes and liver lymphocytes were maintained in RPMI 1640 containing 5% FCS, 50 μM 2-ME, 2 mM glutamine, 0.1 mM nonessential amino acids, 10 mM HEPES, and 0.1 mM sodium pyruvate.
Parental pCC1-YF17D and recombinant pCC1-YF17D/PyCS plasmid DNA were purified by alkaline lysis and CsCl banding. Linearized template was created by XhoI digestion, and was used for in vitro transcription by SP6 RNA polymerase to generate 5′ capped viral genomic RNA (Ambion). Five micrograms of the resulting RNA were electroporated into BHK J cells as previously described . The specific infectivity of the viral RNA was measured by seeding serial dilutions of electroporated cells on monolayers of untransfected BHK J cells, overlaying with 0.6% agarose in 2× medium, and counting viral plaques after 96 hours. Virus stocks were harvested at 48 hours post-electroporation (hpe) (YF17D) or 72 hpe (YF17D/PyCS). Single use aliquots were stored frozen at -80°C until use.
Expression of CSP and YF antigens was assessed by immunofluorescence with anti-PyCS (3F11 monoclonal) and anti-YF E (5E3 monoclonal ), and by Western blot analysis with anti-YF NS4 (C-12 polyclonal ) in addition to the above mentioned antibodies.
P. yoelii (17× NL strain) was maintained by alternate cyclic parasite passage in Anopheles stephensi and infected blood transfer to mice. Sporozoites were obtained by dissection of salivary glands of A stephensi and collected in medium RPMI 1640 at 4°C.
BHK J cells were infected at a multiplicity of infection (MOI) of 1. One hour post-infection (hpi), media was replaced. Supernatants were collected at 24, 48, 72, and 96 hpi and stored frozen at -80°C. Virus-containing supernatants were analyzed by plaque assay as previously described . Briefly, serial 10-fold dilutions were used to infect monolayers of BHK J cells. After 1 hour, cells were overlayed with 0.6% agarose in 2× medium. Plaques were fixed with 7.4% formaldehyde and stained with 0.3% crystal violet at 72 hpi.
For RT PCR, virus was passaged consecutively in BHK J cells with a MOI of 1. After each passage, 150 μl of virus-containing supernatant was harvested and RNA was purified using QIAmp Viral RNA mini kit following the manufacturer's recommendations (Qiagen). RNA was analyzed using the SuperScript III One-Step RT-PCR System (Invitrogen). Passages 1 and 6 were analyzed by sequencing (Geneart).
Mice were primed subcutaneously (s.c.) in 200 μl volume with 2×105 PFU YF17D/PyCS, 2×105 PFU YF17D, or 1× phosphate buffered saline (PBS) solution, or intravenously (i.v.) with 50,000 irradiated sporozoites. Boost consisted of 5,000 irradiated sporozoites given i.v. four weeks following primary immunization or 2×105 PFU YF17D/PyCS given s.c. Challenge was performed by i.v. injection of 250 viable sporozoites at three weeks following boosting. Blood smears were prepared from the tail of infected animals on days 3-10 following the challenge injection to measure parasitemia. Groups that became parasitemic by day 6 were not analyzed further and were considered non-protected. The number of infected red blood cells (iRBC) in a total count of 1000 RBCs was determined by staining with Giemsa (SIGMA). Animals with 0 iRBCs were considered protected.
Mice were euthanized and perfused in situ at room temperature using PBS. The livers were then harvested and placed on cold PBS+2%FBS (Invitrogen). After the removal of the gall bladders, livers were cut into small pieces and pressed gently through a 70 μm cell-strainer (BD Biosciences). The cell suspension was washed twice with PBS+2% FBS and centrifuged at 500×g for 5 min at 4 °C. A maximum of two livers were resuspended in 25 ml of a room temperature 37.5% Percoll solution (Amersham-Pharmacia) and then centrifuged at 700×g for 15 min at room temperature. The cell pellet was washed once with PBS+2%FBS and then lysed in 1-2 ml of red blood cell lysis buffer (GIBCO) for 5 min at room temperature. The remaining cells were washed twice with PBS+2%FBS and concentrations were adjusted to 4×106 cells/ml.
A 15-mer overlapping peptide library for the P. yoelii CS protein (CSP) was synthesized by the Proteomics Resource Center, The Rockefeller University .
Intracellular IFN-γIL2andTNF-α production by CD4+ and CD8+ T cells was evaluated using bulk splenocytes and lymphocytes derived from the livers of immunized animals. Splenocytes and liver lymphocytes were incubated with 2 μM of each CSP peptide pool , the CD8 immunodominant peptide (SYVPSAEQI) or medium alone in the presence of 2 μg/ml of costimulatory αCD28 antibody (clone 37.51). Cells were cultured for 12 hours in the presence of brefeldin A (BD Biosciences), incubated withantiCD16/CD32 antibody to block Fc-γ receptors, and stained with anti–mouse CD4 (clone GK 1.5), CD8 (clone 53-6.7), and CD3 (clone 145-2C11) antibodies for 15 min at 4°C. After fixation with Cytofix/Cytoperm Plus (BD Biosciences), cells were stained for intracellular IFN-γ (XMG1.2) for 15 min at room temperature.
Surface expression of mouse CD11c, CD86 and intracellular expression of P. yoelii CSP were quantified using Pacific Blue-conjugated anti-CD11c (clone N418), PE-conjugated anti-CD86 (clone PO3.1), and monoclonal 488-conjugated anti-PyCS (clone 3F11 conjugated with Alexa Fluor 488, Molecular Probes) antibodies, respectively. For flow cytometry, BmDCs were stained with the antibodies for 15 min at 4°C and after fixation with Cytofix/Cytoperm Plus, cells were stained for intracellular PyCS for 15 min on ice.
All conjugated antibodies were purchased from BD Biosciences or eBioscience, and diluted according to manufacturers recommendations. Data were collected using LSRII or FACS Calibur and analyzed using FlowJo software (Tree Star).
The P. yoelii CS sequence (nt 172-1034) was subcloned into pET21a (Novagen) HindIII and XhoI sites to create a C-terminally His-tagged PyCS expression construct, termed pET21a+PyCS. Tuner competent bacteria (Novagen) were transformed with pET21a+PyCS, and a single colony was picked and grown overnight at 37°C in LB-ampicillin (100 μg/ml), followed by induction (when D.O.600nm reached 0,5) with 2 mM IPTG for 4 hours at 30°C. Cells were collected by centrifugation and resuspended in 5 ml of lysis buffer (50 mM Tris, 200 mM NaCl, 10% glycerol, pH 8.0) per 125 ml culture pellet. PMSF to a final concentration of 1 mM was added and the bacterial suspension was sonicated 5 times for 1 min with 1 min intervals. The lysate was clarified by centrifugation at 28,000 ×g for 45 min and the supernatant was incubated under rotation with 5 ml Ni-NTA resin (Qiagen), which had been previously equilibrated in lysis buffer, for 2 hours at 4°C. After 4 washes with 10 ml wash buffer (50 mM Tris, 500 mM NaCl, 10% glycerol, 30 mM imidazole, pH 8.0), protein was eluted from the Ni-NTA matrix using 5 ml of elution buffer (50 mM Tris, 1M NaCl, 10% glycerol, 500 mM imidazole, pH 8.0) for 1 hour at 4°C. Purified CSP was then dialyzed into PBS, filtered in 0.2 μM filter, and stored at -80.
For the detection of CSP-specific and YFV-specific antibodies, high-binding ELISA plates (Costar) were coated overnight with 5 μg/ml recombinant CSP in PBS or with 50μl of virus-containing supernatant derived from infected BHK J cells, respectively. Plates were then washed three times with PBS containing 0.02% Tween-20 and blocked with PBS-BSA 1% for 1 hour at room temperature. Serial dilutions of sera in PBS-BSA 0.25% were incubated for 2 hours at room temperature before addition of goat anti–mouse IgG Fc-specific antibody conjugated to horseradish peroxidase (1:2,000; Jackson ImmunoResearch Laboratories). ELISA were developed using horseradish peroxidase substrate (Biorad), and OD405nm was measured using a VERSAmax microplate reader (Molecular Devices). Titers represent the highest dilution of serum showing an OD405 >0.1 over background. The results are presented as the log2 antibody titer of each group of mice ± standard deviation.
CD8+ T cells from Thy 1.1+ SYVPSAEQI-specific TCR transgenic mice were purified and carboxyfluorescein succinimidyl ester (CFSE) labeled as described previously . 3-5×106 cells were transferred i.v. to naive BALB/c mice, followed by s.c. infection with YF17D/PyCS or YF17D at 24 hours-post transfer. Cell division was measured in the spleen and liver by CFSE dilution 2 days later. Transgenic T cells were gated using anti-Thy 1.1 (clone OX-7) and anti-CD8 (clone 53-6.7) antibodies (BD Biosciences).
BmDCs were prepared as described above and infected with YF17D/PyCS at a MOI of 5 or exposed to LPS (500 ng per 2×106 cells) in a 24 well culture plate (Costar). Forty-eight hours following infection, most of the media was removed and cells were harvested with cold PBS for FACS analysis as described above.
A recombinant YF17D cDNA was modified to encode the Plasmodium yoelii circumsporozoite (CS) sequence, encompassing nucleotides 172-1034. This sequence was chosen deliberately to include the immunodominant repeated peptides QGPGAP and QQPP, and the well-described class I CTL epitope SYVPSAEQI [30-33]. The CS sequence was inserted in frame into the YF17D cDNA following nucleotides 1-75 of the capsid gene, which contain the genome cyclization sequence required for replication. The foot-mouth disease virus (FMDV) 2A peptide and the mammalian ubiquitin (Ubi) coding sequences were inserted directly following CSP to ensure efficient cleavage from the downstream YF polyprotein (Figure 1A). The CSP-FMDV2A-Ubi cassette was followed by the full-length capsid gene and the remainder of the YFV genome. The resulting recombinant cDNA, pCC1-YF17D/PyCS, was in vitro transcribed to generate infectious viral RNA.
To determine if recombinant YF17D/PyCS was viable and expressed the inserted transgene, infectious RNA was transfected into BHK J cells. Immunofluorescent staining and Western blot analysis of electroporated cells revealed that the recombinant virus expressed accurately processed YF envelope (E) protein and non-structural protein 4B (NS4B), as well as the P. yoelii CSP (Figure 1B-C). To investigate the ability of the recombinant genome to produce infectious virus, the specific infectivity of the transfected RNA was measured and single-step growth curves were generated. YF17D/PyCS remained cytopathic but yielded plaques that were distinctly smaller than those of the parental YF17D virus at 72 hours post infection (hpi) (Figure 2A). Although the recombinant virus had slower kinetics following initial infection, it reached titers approaching those of the parental virus by 96 hpi (Figure 2B). The specific infectivity of RNA derived from the YF17D/PyCS construct was approximately 2-fold lower than the parental YF17D RNA (Figure 2C). To determine if the recombinant genome was genetically stable, YF17D/PyCS was passaged consecutively in BHK J cells at MOI of 1. The presence of the insert was verified by RT PCR and sequencing, and could be detected intact up to 6 passages; PyCS expression became undetectable by passage 7, when we were also unable to amplify the PyCS insert from infected cells (Figure 2D and data not shown). These results demonstrate that a monocistronic YF17D genome encoding a large heterologous gene insertion in the capsid region is viable in cell culture but is genetically stable for only a limited number of passages.
The ability of the recombinant YFV to stably express CSP in culture encouraged us to examine immunogenicity in vivo. To determine if YF17D/PyCS could effectively prime an immune response, we developed a prime-boost immunization regimen using YF17D/PyCS alone or in combination with irradiated sporozoites. Groups of five mice received 2×105 plaque forming units (PFU) of YF17D/PyCS alone or followed by a boost with 5000 irradiated sporozoites or with 2×105 PFU of YF17D/PyCS four weeks later. Control groups were immunized with PBS, or with one (5000) or two doses of irradiated sporozoites (50000 followed by 5000 parasites). Nine days post-boosting, intracellular levels of IFN-γIL2andTNF-α production by peptide-stimulated T cells were measured in three mice from each group. Synergistic induction of IFN-γ was observed in liver-derived CD8+ T cells following the YF17D/PyCS-prime and sporozoite-boost regimen, resulting in cytokine production that was approximately 2-3 fold greater than after two doses of irradiated sporozoites. Furthermore, immunization with single dose of YF17D/PyCS resulted in higher levels of IFN-γ-production than with a single dose of irradiated sporozoites. A second dose of YF17D/PyCS did not significantly increase the production of IFN-γ by CD8+ T cells. IFN-γ-production in CD4+ T cells derived from the liver was also greater in YF17D/PyCS-immunized mice than in irradiated sporozoite-immunized animals. Similar results were observed for T cells isolated from the spleen (data not shown). In addition, a small percentage of CD4+ T cells produced IFN-γ, IL-2 or TNF-α following immunization with YF17D/PyCS (Figure 3). These data indicate that the YF17D/PyCS-based regimen can activate T cell effector functions at levels comparable or superior to high-dose immunization with sporozoites.
T cells are essential for the development of immunity against malaria, and CD8+ T cells targeting P. yoelii CSP are known to eliminate liver stage parasites in an antigen-specific manner . YF17D can infect antigen-presenting cells leading to activation of T cells [12-14], suggesting that the recombinant YF17D virus may be a potent inducer of anti-parasite immunity. We therefore investigated the in vivo proliferation of CSP-specific CD8+ T cells following immunization with YF17D/PyCS. T cells specific for CSP epitope SYVPSAEQI were isolated from a previously described T cell receptor (TCR) transgenic mouse model . Purified T cells were labeled before adoptive transfer into naïve BALB/c mice. Following immunization of the recipient mice with YF17D/PyCS, SYVPSAEQI-specific T cells proliferated extensively in both the liver and the spleen, as determined by decreased CSFE fluorescence intensity in daughter cells. This proliferation was not observed in mice immunized with the parental YF17D (Figure 4). These results suggest that delivery of the CSP by YF17D/PyCS can lead to expansion of T cells specific for malarial epitopes.
Humoral responses in the immunized animals were measured by ELISA for CSP- and YFV-reactive antibodies at fourteen days following initial immunization (pre-boost) and at nine days following the boost (post-boost) (Tables 1 and and2).2). To determine if the recombinant virus was immunogenic in mice, groups of three mice were immunized with 2×105 PFU of YF17D/PyCS- or YF17D- prime followed by a boost with 5,000 irradiated sporozoites. Control mice were immunized with PBS. Specific anti-YFV antibodies were found in mice that received either the recombinant YF17D/PyCS or YF17D alone but not in control mice (Table 1).
To determine if the recombinant virus induced anti-CSP antibodies, we immunized groups of three mice with 2×105 PFU YF17D/PyCS alone or followed by a boost with 5,000 irradiated sporozoites. Control groups were immunized with PBS, or with one (5000) or two doses of irradiated sporozoites (50000 followed by 5000 parasites). Low levels of anti-CSP antibodies were detected following primary immunization with YF17D/PyCS, and an increase in antibody titers was observed following the boost with irradiated sporozoites (>2.5 fold). Antibody levels induced following the YF17D/PyCS-prime, irradiated sporozoite-boost regimen, however, were not significantly different when compared to titers induced after a single immunization with 5000 irradiated sporozoites alone (p>0.01, One-way ANOVA). Immunization with two doses of irradiated sporozoites yielded antibody titers that were significantly higher than those observed after one immunization with YF17D/PyCS followed by irradiated sporozoites or irradiated sporozoites alone (p<0.01, One-way ANOVA) (Table 2). These results indicate that YF17D/PyCS induces only low levels of anti-CSP antibodies and that a heterologous boost is critical to augment the antibody response in mice.
To examine the potential of recombinant YFV to confer protection against challenge, we repeated the immunization schedules described above with groups of 10 mice and challenged the mice with 250 viable sporozoites three weeks following the final boost. Blood smear analysis of the immunized mice at 10 days post-challenge showed that all ten animals receiving 2×105 PFU of YF17D/PyCS followed by a boost with 5,000 irradiated sporozoites were completely protected against homologous challenge. Four out of ten animals administered a low dose (5000) of irradiated sporozoites alone were protected and all ten animals receiving two doses of irradiated sporozoites (50000 followed by 5000 sporozoites) were protected. None of the animals inoculated with a single dose of YF17D/PyCS or PBS alone were protected (Table 3). These results show that the YF17D/PyCS-prime, irradiated sporozoite-boost regimen is as effective as multiple doses of irradiated sporozoites at conferring immunity to P. yoelii challenge in mice.
Previous studies have shown that YF17D is capable to productively infect human DCs ex vivo, and infected DCs can process and present foreign antigens to CD8+ T-cells [12-14]. To determine if mouse DCs were susceptible to YF17D/PyCS infection or activation, we prepared BmDCs and inoculated them with a MOI of five. DCs were also exposed to LPS as a positive control. Thirty-six hours after infection, BmDC were analyzed by flow cytometry with anti-CD11c DC marker, anti-CD86 DC activation marker, and anti-CSP (3F11) antibodies. Even though noticeable infection was not observed, upregulation of CD86 was apparent in cells that had been exposed to YF17D/PyCS or LPS (Figure 5). These results suggest the recombinant YF17D/PyCS is an effective activator of antigen presenting cells.
Here we describe the construction and characterization of a recombinant YF17D virus expressing CSP (amino acids 57-344) from Plasmodium yoelii. A novel insertion site within the YF17D genome was explored, allowing creation of a stable recombinant virus. Previous studies have shown that manipulation of the YF17D capsid is well tolerated as long as the 5′ cyclization sequence, which forms an essential interaction with a region at the 3′ end of viral genome, is unperturbed [22, 35, 36]. We maintained the cyclization sequence upstream of the inserted P. yoelii gene, and recoded the duplicated N-terminal portion of the capsid to ablate the second cyclization site. Processing of PyCS from the YF17D polyprotein was achieved by insertion of the FMDV 2A peptide followed by ubiquitin, as has previously been described for hepatitis C virus (HCV) reporter genomes . We determined that the YF17D/PyCS genome exhibited slightly delayed replication but was genetically stable throughout 6 passages in cell culture. Since were we able to harvest YF17D/PyCS virus with titers comparable to that of YF17D, we hypothesize that the altered kinetics may result from the increased length of the recombinant genome, or from unpredicted factors such aberrant RNA secondary structures. Although, these recombinant viruses were genetically stable for only a limited number of passages in vitro, and in vivo stability can be unpredictable, virus stocks suitable for vaccination can be generated directly from electroporated cells without passaging to minimize loss of the CSP sequences.
We investigated the ability of the recombinant virus to elicit anti-CSP immune responses and protect against sporozoite challenge in adult mice. Inoculation of mice with 2×105 PFU of YF17D/PyCS, followed by boosting with 5000 irradiated sporozoites conferred protection against infection with live P. yoelii, while neither of the regimen components alone were completely protective. Previously, YF17D expressing a single CTL H2Kd restricted epitope derived from P. yoelii CSP was shown to induce long-lived IFN-γ-producing CD8+ T cells, and achieved sterile immunity in 9 out of 10 mice immunized with 5×105 PFU of virus followed by 10000 irradiated sporozoites . In the present study, half the dose of recombinant YFV and half the number of irradiated sporozoites resulted in increased efficacy against challenge with three times the number of viable sporozoites. The higher protective efficacy of the YF17D/PyCS genome observed in our study may be a result of the broader repertoire of epitopes represented by the larger CSP insert. In addition to CD8+ T cell epitopes, such as immunodominant aa 280-288 [31, 34] and subdominant aa 58-67, we included defined CD4+ T cell epitopes (aa 57-70 and aa 59-79), all of which have been associated with reduced parasitemia [39-41]. Given that adult mice are generally resistant to YF17D replication , the low level of immunity induced by the recombinant alone is not surprising. We speculate that experimental immunization of rhesus macaques, a broadly accepted model for yellow fever studies, would yield an even more pronounced anti-CSP response.
We observed induction of IFN-γ–producing CD4+ and CD8T cells after YF17D/PyCS immunization, which was further enhanced by a low dose of irradiated sporozoites. Extensive proliferation of CSP epitope-specific T cells from TCR-transgenic mice suggested the expansion included cell subsets targeting CS sequences. Notably, the IFN-γ response to the YF17D/PyCS-based regimen was remarkably superior to that induced by two doses of irradiated sporozoites in our study. High doses of irradiated or attenuated sporozoites, which elicit sterile immunity in experimentally infected rodents, primates, and human volunteers, mediate protection primarily through CD8+ T cell activation and the production of IFN-γ [43-45]. The presence of CD8+ T cells is similarly essential for immunity against liver-stage parasites in murine models of malaria [43, 46]. A critical role for IFN-γ producing CD4+ T cells has also been highlighted in murine and in human malaria. For instance, adoptive transfer of a CD4+ T-cell clone that recognizes an epitope of P. yoelii CSP protects mice against sporozoite challenge [40, 47]. Likewise, elevated levels of IFN-γ producing CD4+ T cells have been associated with protection from placental malaria . IFN-γ is thought to inhibit parasite development thereby reducing re-infection, and high levels of IFN-γ have been associated with protection from parasitemia, clinical malaria, and anemia [49-51]. Finally, in contrast to the irradiated sporozoite immunizations, we observed a multifunctional response to YF17D/PyCS vaccination, in which IL-2 and IFN-γ-producing CD4+ T-cells were detected. IL-2 has also been associated with reduced parasitemia in infected individuals . The strong role of the T cell response in anti-Plasmodium immunity was emphasized by the observation that the YF17D/PyCS-based regimen conferred protection despite significantly low-levels anti-CSP antibodies as compared to inoculation with irradiated sporozoites.
The efficient priming ability of the recombinant YF vaccine may reflect the activity of DCs. Although YF17D is known to productively infect human DCs [12, 20], productive infection of mouse DCs or macrophages has not been reported. Mouse BmDCs, however, appeared to become activated upon exposure to the recombinant YFV, as measured by the upregulation of CD86. Previous studies have shown that exposure to YFV induces upregulation of CD86 in infected human DCs and in DCs that are not productively infected . Activation of exposed but uninfected mouse BmDCs might be the result of abortive viral entry or binding to an unknown viral receptor.
In conclusion, we have developed a recombinant YF17D capable of inducing a strong T-cell mediated immune response targeting P. yoelii in adult mice. Effective priming by YF17D/PyCS substantially reduces the number of irradiated sporozoites required to confer immunity to mice. Fewer doses of a vaccine could result in a more cost-effective schedule, could reduce the number of vaccines in already crowded childhood vaccine schedules and may be especially beneficial for developing countries that have difficulty delivering a complete multiple-dose schedule. This reduction and potential elimination of the highly immunogenic sporozoite boost may hasten development of a much-needed malaria vaccine.
We thank Catherine Murray, John Schoggins, Svetlana Marukian, Maryline Panis, Anesta Webson, David Bowman, and Megan Holz for technical and editorial assistance.
This research was supported by funds from Foundation for the National Institutes of Health through the Grand Challenges in Global Health initiative, Marie-Josee and Henry Kravis Fellowship at The Rockefeller University, Greenberg Medical Research Institute, and The State of São Paulo Research Foundation (FAPESP).
Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.