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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
Nature. Author manuscript; available in PMC 2010 September 7.
Published in final edited form as:
PMCID: PMC2935199
NIHMSID: NIHMS231110

Generation of functional multipotent adult stem cells from GPR125+ germline progenitors

Abstract

Adult mammalian testis is a source of pluripotent stem cells1. However, the lack of specific surface markers has hampered identification and tracking of the unrecognized subset of germ cells that gives rise to multipotent cells2. Although embryonic-like cells can be derived from adult testis cultures after only several weeks in vitro1, it is not known whether adult self-renewing spermatogonia in long-term culture can generate such stem cells as well. Here, we show that highly proliferative adult spermatogonial progenitor cells (SPCs) can be efficiently obtained by cultivation on mitotically inactivated testicular feeders containing CD34+ stromal cells. SPCs exhibit testicular repopulating activity in vivo and maintain the ability in long-term culture to give rise to multi-potent adult spermatogonial-derived stem cells (MASCs). Furthermore, both SPCs and MASCs express GPR125, an orphan adhesion-type G-protein-coupled receptor. In knock-in mice bearing a GPR125–β-galactosidase (LacZ) fusion protein under control of the native Gpr125 promoter (GPR125–LacZ), expression in the testis was detected exclusively in spermatogonia and not in differentiated germ cells. Primary GPR125–LacZ SPC lines retained GPR125 expression, underwent clonal expansion, maintained the phenotype of germline stem cells, and reconstituted spermatogenesis in busulphan-treated mice. Long-term cultures of GPR125+ SPCs (GSPCs) also converted into GPR125+ MASC colonies. GPR125+MASCs generated derivatives of the three germ layers and contributed to chimaeric embryos, with concomitant downregulation of GPR125 during differentiation into GPR125 cells. MASCs also differentiated into contractile cardiac tissue in vitro and formed functional blood vessels in vivo. Molecular book marking by GPR125 in the adult mouse and, ultimately, in the human testis could enrich for a population of SPCs for derivation of GPR125+ MASCs, which may be employed for genetic manipulation, tissue regeneration and revascularization of ischaemic organs.

The genetic and phenotypic profile of the specific subset of spermatogonial cells that converts into multipotent adult cells is poorly defined. We discovered a potential stem and progenitor cell surface marker (GPR125) expressed on the adult testis, while evaluating a large series of mouse knockouts3. The endogenous Gpr125 locus was altered by joining the amino-terminal putative extracellular and first transmembrane domains to β-galactosidase (Supplementary Fig. 2). Homozygous mice were grossly normal and fertile. Histochemical examination of the post-natal testis with the β-galactosidase substrate X-gal (5-bromo-4-chloro-3-indolyl-β-D-galactoside) revealed that GPR125 expression was restricted to the seminiferous tubules and was confined within the first layer of cells adjacent to the basement membrane of the peritubular cells (Fig. 1a–c). Immunohistochemistry revealed GPR125 expression only in spermatogonia (Fig. 1e).

Figure 1
Restricted GPR125 expression in adult mouse testis and derivation of multipotent cells from spermatogonial progenitor cells (SPCs)

As spermatogenesis proceeds along the length of the seminiferous tubule, characteristic sets of differentiating cell types are seen together in a given cross-section, allowing such cross-sections to be categorized into twelve stages4. Expression of GPR125 was greatest at later stages (that is, VII–VIII) with a nadir in earlier stages (that is, IV–V), as analysed either by promoter activity (X-gal) or by immunostaining (in wild-type mice; Fig. 1c–e). To quantify expression of GPR125–LacZ in the Gpr125lacZ/lacZ spermatogonia, staining was performed with fluorescein di-D-galactopyranoside (FDG), followed by flow cytometry. Freshly dissociated adult Gpr125lacZ/lacZ seminiferous tubules yielded ~35% LacZ+ cells (Fig. 1f). The high yield of LacZ+GPR125+ cells may be a result of our preparation of testicular tissue—in which contaminating interstitial somatic cells and spermatids are lost during washing steps—combined with the high sensitivity of the FDG assay5.

To determine whether LacZ+GPR125+ cells represent self-renewing spermatogonial cells with the capacity to generate MASCs, we sought to recapitulate in vitro the native niche that supports efficient self-renewal of these cells. We discovered that the LacZ+GPR125+ cells reside in close proximity to the CD34+ peritubular cells6, indicating that interaction of these two cell types may be essential for expansion of the GPR125+ SPCs (Fig. 1g and Supplementary Fig. 3a, b). To culture GPR125+ cells, we established primary mitotically inactivated adult mouse testicular stromal cells containing CD34+ putative peritubular cells (CD34+MTS), because our initial attempts using mouse embryo fibroblasts (MEFs) were unsuccessful. Amongst the CD34+ stromal cells were also α-smooth-muscle-actin-positive (ACTA2+) and vimentin-positive (VIM+) cells that together supported derivation and long-term proliferation of adult SPCs from mouse testes of various ages (up to 1 yr) and genetic backgrounds in >90% of attempts (Fig. 1g, inset and Supplementary Fig. 3c, d). The adult spermatogonial cultures displayed heterogeneous colony size, with frequent formation of massive proliferating colonies, exponential overall growth, and ~30% of cells in S phase (Fig. 1h, j, and Supplementary Fig. 4a–c). Adult SPC lines were also derived from mice displaying green fluorescence in all tissues (see ref. 7) and were serially passaged six times in typical fashion on CD34+MTS. This revealed expansion of SPCs and near total (>99%) depletion of any somatic green-fluorescent-protein-positive (GFP+) cells outside of the characteristic spermatogonial stem cell-like colonies, suggesting loss of the non-germline contaminants (Supplementary Fig. 4c). The SPC lines expressed typical mouse germ-lineage markers, including germ-cell nuclear antigen (GCNA1), DAZL and MVH (also known as DDX4) (Supplementary Fig. 4d–f)810. Notably, the colonies expressed the well-characterized marker PLZF (also known as ZBTB16), which identifies undifferentiated spermatogonia (Fig. 1i)11,12. Evidence of bona fide stem cell activity within the SPC pool (cultured for more than one year) was revealed by the ability of cells to participate in reconstitution of spermatogenesis in busulphan-treated host mice (see Fig. 2)13.

Figure 2
Characterization and multipotent derivatives of Gpr125lacZ/lacZ SPC lines

Prior studies have found that embryonic stem cell (ESC)-like cells arose in culture either from neonatal testicular cells by spontaneous conversion in the presence of glial-cell-line-derived neurotrophic factor (GDNF) and leukaemia inhibitory factor (LIF) on MEFs14, or in adult spermatogonial stem cells (SSCs) maintained in the absence of GDNF within four weeks after the initiation of spermatogonial colonies1. We found that long-term culture of adult SPCs generated distinct colonies of MASCs from cells that were originally cultured on the CD34+MTS feeder layers for more than three months (Fig. 1k, l). The emergence of MASC colonies was heralded by a distinct morphologic change in a subset of SPC colonies (Supplementary Fig. 4g). Putative MASC colonies, resembling ESCs, were mechanically transferred from CD34+MTS to MEFs for MASC expansion in the undifferentiated state (Fig. 1k, and Supplementary Fig. 1)15. Whereas the pluripotency marker OCT4 (also known as POU5F1) protein was undetectable in SPCs (data not shown), we found unequivocal OCT4 expression in the nuclei of MASCs that were expanded (15 passages before cryopreservation) on MEFs (Fig. 1l) and that were capable of differentiation into multiple lineages in vitro, including rhythmically contractile cardiogenic tissue (Supplementary Fig. 5a–d and Supplementary Movie 1). MASCs gave rise to teratomas (in 9/9 attempts) when injected subcutaneously in NOD-SCID mice (Supplementary Fig. 5e–h). The expression of LacZ in both Gt(ROSA26)Sor-lacZ16 MASCs, and the resultant teratomas, excluded the possibility of a multipotent mesenchymal cell originating from the wild-type, mitomycin-C-inactivated feeders. Furthermore, MASCs cloned from single cells were similarly competent to form tri-lineage teratomas and to contribute to chimaeric embryos on blastocyst injection (see Fig. 3).

Figure 3
GPR125–LacZ MASCs exhibit multipotency and can form functional vessels

To determine whether GPR125 is expressed on SPCs, testes from Gpr125+/lacZ and Gpr125lacZ/lacZ mice were used to derive SPC lines that were propagated on CD34+MTS. Refractile, cobblestone colonies reminiscent of SSCs appeared within one week, and large proliferative colonies were seen within 3–4 weeks that exhibited exponential clonal growth, and culture wells could be de-populated with complete re-growth of colonies (Fig. 2a, b). Maintenance of the germ-cell phenotype was confirmed by immunohistochemistry for GCNA and DAZL (Fig. 2c)17, but c-KIT was absent by flow cytometry (Supplementary Fig. 6a). Strikingly, GPR125–LacZ SPCs maintained GPR125 expression after multiple passages in vitro (Fig. 2a, inset) and are hereafter referred to as GPR125+ SPCs (GSPCs). To determine the frequency of repopulating cells, limiting dilution analysis was performed using GFP-labelled GPR125–LacZ GSPCs (from Gpr125lacZ/lacZ mice) on cells that were cultured beyond 9 months, revealing 0.23 (95% confidence interval, 0.19–0.27) colony forming units (c.f.u.) per cell or 1 c.f.u. for every 4–5 GSPCs (Fig. 2d). All emerging colonies derived from the testes of Gpr125lacZ/lacZ mice expressed lacZ, suggesting that the GSPCs are clonagenic (Fig. 2d).

The molecular identity of Gpr125lacZ/lacZ GSPCs in long-term culture was confirmed by quantitative PCR (Fig. 2e, and Supplementary Fig. 7). Among the transcripts expressed in Gpr125lacZ/lacZ GSPC cultures were germ-cell-specific genes, including Dazl and Mvh10,17. To rule out spontaneous spermatogenic differentiation of the cultured GSPCs18, we surveyed transcripts characteristic of differentiated germ cells and noted diminished or absent levels for transcripts such as Sox17, Tnp1, Adam2, Prm1 and Pgk2 (ref. 19). These data suggested that repopulating GSPCs were of germ-cell origin but remained undifferentiated. Even after in vitro propagation for over one year, GSPCs revealed a transcriptional profile highly reminiscent of spermatogonial stem cells (Fig. 2e, and Supplementary Fig. 7). Various cell surface markers used for isolation of SSCs were increased at the mRNA level in GSPCs: Itga6 (~18-fold), Tacstd1 (~5-fold), Cd9 (~15-fold) and Gfra1 (~128-fold)2022. Similarly, genes with preferential promoter activity in undifferentiated cells, including Stra8 and Oct4, were detectable albeit at lower levels in the GPR125+ cells. Therefore, our culture technique yields undifferentiated spermatogonia, which like spermatogonia in vivo, express GPR125.

To interrogate the repopulating potential of GSPCs in vivo, we then evaluated the capacity of GFP-labelled Gpr125lacZ/lacZ GSPCs to restore spermatogenesis within busulphan-treated C57Bl6 host mouse testes13. Within 2–3 months after transplantation, robust GFP+ Gpr125lacZ/lacZ germ-cell colonies were detectable within the host seminiferous tubules (Fig. 2f, and Supplementary Fig. 8a). These colonies contained populations of GFP+ cells along the basement membrane, exhibiting typical spermatogonial morphology, and smaller round GFP+ cells located more centrally to tubular lumen (Fig. 2f, and Supplementary Fig. 8b–g). X-gal staining confirmed co-expression of GPR125 (LacZ+) in a small subset of the GFP-labelled, transplanted cells along the basement membrane (Fig. 2g, and Supplementary Fig. 9a–e), recapitulating the spatial expression pattern in the GPR125–LacZ testes (see Fig. 1). Importantly, GFP+ spermatids were seen in donor-colonized tubules but not in adjacent tubules containing residual, host-derived spermatogenesis, confirming the presence of true stem cell activity within the long-term Gpr125lacZ/lacZ GSPC cultures (Fig. 2h, and Supplementary Fig. 8h). PCR for GFP detected donor-derived sperm in the epididymis draining the transplanted testis but not in negative controls (data not shown).

The origin of multipotent stem cells in the adult testis is not clear23. Therefore, we sought to formally prove that GSPCs could indeed generate multipotent cells, even after long-term expansion in vitro. The spontaneous emergence of MASCs was observed in the Gpr125lacZ/lacZ cultures that were initially propagated for more than 3 months. These Gpr125lacZ/lacZ MASCs had a high nuclear-to-cytoplasmic ratio, formed refractile colonies, and could be split in a ratio of ~1:8 in mouse embryonic stem cell medium every 2–3 days (Fig. 2i; passaged >30 times before cryopreservation). The majority of cells had a normal karyotype, and no evidence of clonal cytogenetic abnormalities was found for either Gpr125lacZ/lacZ MASCs or Gt(Rosa26)Sor-lacZ MASCs (data not shown). Notably, the majority of cells within the colonies were highly positive for GPR125 expression and also uniformly immuno-positive for OCT4 within the nucleus (Fig. 2j). FDG-labelling revealed more than 99% of both Gpr125lacZ/lacZ GSPCs and MASCs to be GPR125+by β-galactosidase activity (Fig. 2k), suggesting that GPR125 is associated more universally with the stem- and progenitor-cell phenotype.

The multipotency of these Gpr125lacZ/lacZ MASCs was first assessed by formation and differentiation of embryoid bodies in vitro24. Within seven days after re-plating, embryoid bodies exhibited a distinct pattern of GPR125 expression, with distinct borders between GPR125+ and GPR125 areas (Supplementary Fig. 10a, b). The resultant colonies contained HNF3β+ (FOXA2+) cells derived from endoderm or ectoderm, cytokeratin-positive (KRT+) or GFAP+ cells derived from ectoderm, and brachyury-positive or skeletal-muscle-myosin-positive (MYH2+) cells derived from mesoderm (Fig. 3a, b).

When GPR125lacZ/lacZ MASCs were implanted subcutaneously in NOD-SCID mice, the resultant teratomas (14/14 attempts) similarly exhibited GPR125 expression in a lineage-specific manner, implying loss of GPR125 in certain differentiated cell types (Fig. 3c and Supplementary Fig. 11). In fact, these teratomas were reminiscent of GPR125–LacZ embryos, in which GPR125 expression is present in most but not all tissues and subsequently lost over time (see Fig. 3h, Supplementary Fig. 12). Lineage analysis of MASC teratomas demonstrated morphologic and immunologic evidence for tissue derivatives of all three germ layers, including mucin-positive (Muc5ac+) endoderm, GFAP+ neuroectoderm, and mesodermal chondrocytic, myoid, and vascular cells (Fig. 3d–f).

The ability to form chimaeric animals has been used to demonstrate multipotency of germ-cell derivatives2. We therefore performed blastocyst injections with cloned Gpr125lacZ/lacZ MASCs and found 8 chimaeric embryos out of 37 evaluated (22%). Importantly, the expression pattern of GPR125 in the C57Bl6 (host)/Gpr125lacZ/lacZ (donor) chimaeric embryos partially recapitulated what was seen in heterozygous knock-in Gpr125+/lacZ embryos, with prominent signal in developing ossification centres (Fig. 3g, h, and Supplementary Fig. 12e, f). In addition, LacZ+ cells were also detected in the chimaeric gut and other tissues that are known to harbour GPR125+ cells in non-chimaeric embryos (Supplementary Table 2 and Supplementary Fig. 12). These data indicate that generation of GPR125+ MASCs from GSPCs results in the maintenance of the expected global expression pattern of the Gpr125 gene. As such, lineage-specific derivatives of MASCs may have the essential set of genetic and epigenetic instructions that are critical for autologous organ regeneration.

To this end, we examined the ability of MASCs to differentiate into endothelial cells. An extensive network of vessel-like, lumen-containing VE-cadherin+ (CDH5+) structures were formed in vitro from MASC embryoid bodies after 22 days of differentiation (Fig. 3i, and data not shown). To determine whether GPR125+MASCs could differentiate into functional vessels in vivo, GPR125+ MASCs were transduced with a lentiviral vector expressing GFP under control of the promoter for the endothelial-specific marker VE-cadherin (ref. 25). Teratomas formed in NOD-SCID mice from such transduced MASCs contained donor-derived GFP+ blood vessels, continuous with the host circulation, as shown by perfusion-based staining and the presence of red blood cells within the vessels (Fig. 3j–l).

We asked next whether MASCs use the same molecular machinery for multipotency as ESCs. Expression analysis of Gpr125lacZ/lacZ MASCs compared with mouse ESCs, GSPCs or MEFs revealed high levels of Oct4, Nanog and Sox2 in both MASCs and ESCs (Fig. 4a). Minimal expression of typical SSC markers, including Plzf, Ret and Stra8, was seen in MASCs, which, as expected, were high in Gpr125lacZ/lacZ GSPCs. Unexpectedly, certain key germ-lineage transcripts (for example, Dazl) were nearly absent in MASCs, as were some canonical mouse ESC transcripts (for example, Gdf3, Esg1 (also known as Dppa5) and Rex1; Fig. 4b). The differences in expression of these genes and others (for example, Nog and brachyury) suggest that MASCs constitute a distinct stem cell type from that reported in ref. 1.

Figure 4
Gpr125lacZ/lacZ MASCs have an expression profile different from mouse embryonic stem cells

Here, we have identified GPR125 as a surface marker for self-renewing, clonagenic, cKITPLZF+ spermatogonial progenitor cells (GSPCs), with the capacity for both repopulating the testis and generating GPR125+ MASCs. Recent evidence indicates that spermatogonial progenitor cells can manifest stem cell activity26. This indicates that GPR125+cKITPLZF+DAZL+ GSPCs may not only be endowed with spermatogonial stem cell activity but may also perform as undifferentiated spermatogonial cells that can convert into GPR125+cKIT+PLZFDAZLOCT4+MASCs. These data pinpoint GPR125+ spermatogonial cells as the cellular ancestors of MASCs (see Supplementary Fig. 1). Differentiation of GPR125+ MASCs into GPR125 tissues qualifies GPR125 expression as a useful marker for tracking differentiation and lineage-specification of stem and progenitor cells.

The precise molecular and cellular pathways governing the emergence of MASC colonies remain unclear. Although MASCs and ESCs have identical morphological characteristics and are both multi-potent, capable of giving rise to teratomas and chimaeric animals, there are major differences at the transcriptional level that distinguish these two cell types (Fig. 4c). Notably, unlike the ESC-like cells derived from STRA8+ SSCs1, GPR125+ MASCs lack the molecular signature of ESCs but mimic other multipotent adult stem cells, such as multipotent adult progenitor cells (MAPCs)27. Our data, therefore, imply that multipotency may be driven by multiple unique sets of signals, even in the absence of gene products typically associated with stem cells (for example, Gdf3, Esg1 and Rex1). Also, in contrast to a prior report1, the maintenance of long-term cultures of GSPCs was dependent on GDNF and was therefore necessary for the subsequent emergence of MASCs. Therefore, culture conditions may influence the ultimate multipotent phenotype.

GPR125 expression in undifferentiated cells and early progenitors and its subsequent downregulation on terminal differentiation raises the intriguing possibility of exploiting surface expression of GPR125 to isolate human SSCs and GSPCs. Recent data demonstrated the in vitro differentiation of endothelium from multipotent cells derived from the neonatal testis28. We extend these observations by showing that GPR125+ MASCs can generate functional vasculature in vivo. Taken together, these data indicate that GPR125+ MASCs could be used therapeutically for the generation of functional autologous vessels for revascularization. However, as germline stem and progenitor cells may have alterations in genomic imprinting in certain genes compared with adult somatic cells14, the use of these cells for therapeutic purposes should proceed with caution and extensive pre-clinical experimentation.

METHODS SUMMARY

SSC, MASC and feeder-cell culture

C57Bl6 mice aged 4–12 wks served as donors for mixed primary testicular feeder cells, which were expanded following enzymatic digestion of the seminiferous tubules. Feeder cells were treated with mitomycin-C before use for stem cell culture. Mouse GSPCs were obtained from enzymatically dissociated seminiferous tubules from mice aged 3 weeks to 8 months and were plated in StemPro-34 (Invitrogen) with the modifications of ref. 29. GSPCs were serially passaged onto fresh mitomycin-C-treated feeders every 2–8 wks. Morphologically atypical transitional colonies of GSPCs were mechanically removed from the plate after >2 wks in culture and re-plated in the same medium or embryonic stem cell (ESC) medium on mitomycin-C-inactivated MEF to obtain MASC lines.

GPR125–LacZ mice

VelociGene technology was employed for production of Gpr125lacZ/lacZ mice as previously described3. Briefly, targeting vectors were generated using a bacterial artificial chromosome (BAC) and contained Gpr125 in which exons 16–19 were deleted and replaced in-frame with lacZ, as a reporter gene, and neomycin, as a selectable marker. Targeting vectors were electroporated into ESCs. Clones that were properly targeted were confirmed by real-time PCR-based loss-of-native-allele assay3, using primers listed in Supplementary Information. Chimaeric mice were generated by blastocyst injection of ESCs and backcrossed to C57Bl6/J to produce heterozygote breeding pairs.

Additional methods

Additional methods, including histochemistry, immunostaining, flow cytometry following FDG-labelling, quantitative (q)PCR, lentivirus preparation and spermatogonial transplantation are presented in the Methods.

Full Methods and any associated references are available in the online version of the paper at www.nature.com/nature.

Supplementary Material

Supplemental

Acknowledgments

This work was supported by the Howard Hughes Medical Institute, Ansary Stem Cell Center for Regenerative Medicine and Memorial Sloan Kettering Cancer Center T32 grant (M.S.), an AACR–Genentech BioOncology Fellowship for Cancer Research on Angiogenesis (M.S.), the Heed Foundation (S.C.), the International Retinal Research Foundation (S.C.) and National Heart, Lung and Blood Institute grants (S.R.). We thank M. Hardy, P. Schlegel, Marc Goldstein, A. Brivanlou and S. Noggle for critical input. We are grateful to G. Enders for providing anti-GCNA antibody. We thank D. S. Johnston, G. Linkov and G. Zlotchenko for technical assistance.

Footnotes

Supplementary Information is linked to the online version of the paper at www.nature.com/nature.

Author Information Reprints and permissions information is available at www.nature.com/reprints. The authors declare competing financial interests: details accompany the full-text HTML version of the paper at www.nature.com/nature.

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