Smad3-Deficient Male Mice Have Lower Levels of LHβ and FSHβ mRNA
Total RNA from individual pituitary was reverse transcribed and LHβ, FSHβ, and GAPDH cDNA were quantified using real-time PCR. For each animal, the amount of LHβ or FSHβ mRNA was calculated from a standard curve performed simultaneously. The amount was compared with the amount of GAPDH also calculated from its standard curve. n = 9 animals per group; the level of LHβ/GAPDH ratio in +/+ animals was set to 1, to allow comparison of the change from wild type. #, Significant difference from the wild-type littermates by Student’s t test: P = 0.0007 for LHβ and P = 0.008 for FSHβ.