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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
Mol Endocrinol. Author manuscript; available in PMC 2010 September 2.
Published in final edited form as:
Mol Endocrinol. 2005 October; 19(10): 2610–2623.
Published online 2005 June 16. doi: 10.1210/me.2005-0047

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Smad3-Deficient Male Mice Have Lower Levels of LHβ and FSHβ mRNA

Total RNA from individual pituitary was reverse transcribed and LHβ, FSHβ, and GAPDH cDNA were quantified using real-time PCR. For each animal, the amount of LHβ or FSHβ mRNA was calculated from a standard curve performed simultaneously. The amount was compared with the amount of GAPDH also calculated from its standard curve. n = 9 animals per group; the level of LHβ/GAPDH ratio in +/+ animals was set to 1, to allow comparison of the change from wild type. #, Significant difference from the wild-type littermates by Student’s t test: P = 0.0007 for LHβ and P = 0.008 for FSHβ.

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