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Effects of AVT upon the nocturnal increase of EOD rate in breeding males of Brachyhypopomus gauderio in different social contexts. Nocturnal EOD rate increase (NDI) of breeding males from male–female (reproductive context) and male–male (agonistic context) dyads injected with saline 65min before the artificial sunset (Protocol II, day 2), and the same breeding males injected with AVT (Protocol II, day 3, 10 μg/gbw, 65min before the artificial sunset). Bars represent medians (IQR) of night–day indexes (NDI, see Materials and Methods). * p <0.05, Mann–Whitney U test. ns: non-significant.